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    Cell Signaling Technology Inc py erk
    Py Erk, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/py erk/product/Cell Signaling Technology Inc
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    py erk - by Bioz Stars, 2021-05
    86/100 stars
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    acea  (Tocris)
    92
    Tocris acea
    <t>CB1</t> activation promoted bone marrow-derived monocyte/macrophage (BMM) polarization toward M1 through G(α) i/o , RhoA, and ERK1/2. BMMs were pre-treated with pertussis toxin (20 ng/mL), Y27632 (10 µmol/L), SB203580 (10 µmol/L), PD98059 (10 µmol/L), compound C (10 µmol/L) for 1 h, and followed by 1 µmol/L <t>ACEA</t> treatment for another 6 h. CD86 mRNA expression (A) and M1 other gene signatures (B–D) were evaluated by RT-qPCR. * P
    Acea, supplied by Tocris, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/acea/product/Tocris
    Average 92 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    acea - by Bioz Stars, 2021-05
    92/100 stars
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    95
    Cell Signaling Technology Inc erk1 2
    Determination of factors critical to lysosome purification. (A) Effect of bead sizes using beads from Creative Diagnostics. The same amount of PNS was incubated with streptavidin beads of different diameters as indicated for 5 min. The volume of the beads for each size was adjusted to have the same binding capacity. Lysosome abundance in the purified products was determined by western blotting for LAMP2, showing that 1 µm diameter beads are the most efficient. (B) Comparison of the effect of bead sizes on lysosomal purification efficiency using beads from Thermo Fisher Scientific. Western blot analyses of LAMP2 from PNS and products of purification of Lyso-2Strep using 25 µm and 1 µm streptavidin beads. (C) Purity of the lysosome preparation. Western blot analyses of PNS and products after purification with Lyso-2Strep using 1 µm streptavidin beads. Intracellular organelle markers used were: LAMP1 and LAMP2 (lysosomes, Lyso), SDHA and TOM20 (mitochondria, Mito), SLC27A2 and catalase (peroxisomes, Pex), PDI and SERCA (endoplasmic reticulum, ER), Golgin-97 and GOLM1 (Golgi), ATP1A1 and PMCA2 (plasma membrane, PM), S6K and <t>ERK1/2</t> (cytosol, Cyt). The purified lysosomes are free of contamination from other organelles. (D) Intactness of the lysosome preparation. Fluorescence images of lysosomes isolated from HeLa cells expressing Lyso-2Strep (green), eluted from streptavidin beads with 20 mM biotin and then labeled with LysoTracker (red). Scale bar: 20 μm. (E) Effect of incubation time and bead abundance. The same amount of PNS (100 µl) was incubated with 30 µl or 60 µl of 1 µm diameter streptavidin beads (Thermo Fisher Scientific) for different time periods as indicated. The relative recovery efficiency of lysosomes was determined by comparing the density of LAMP2 to that of PNS on the same western blots.
    Erk1 2, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/erk1 2/product/Cell Signaling Technology Inc
    Average 95 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    erk1 2 - by Bioz Stars, 2021-05
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    90
    Sino Biological kallikrein 13 klk13
    Determination of factors critical to lysosome purification. (A) Effect of bead sizes using beads from Creative Diagnostics. The same amount of PNS was incubated with streptavidin beads of different diameters as indicated for 5 min. The volume of the beads for each size was adjusted to have the same binding capacity. Lysosome abundance in the purified products was determined by western blotting for LAMP2, showing that 1 µm diameter beads are the most efficient. (B) Comparison of the effect of bead sizes on lysosomal purification efficiency using beads from Thermo Fisher Scientific. Western blot analyses of LAMP2 from PNS and products of purification of Lyso-2Strep using 25 µm and 1 µm streptavidin beads. (C) Purity of the lysosome preparation. Western blot analyses of PNS and products after purification with Lyso-2Strep using 1 µm streptavidin beads. Intracellular organelle markers used were: LAMP1 and LAMP2 (lysosomes, Lyso), SDHA and TOM20 (mitochondria, Mito), SLC27A2 and catalase (peroxisomes, Pex), PDI and SERCA (endoplasmic reticulum, ER), Golgin-97 and GOLM1 (Golgi), ATP1A1 and PMCA2 (plasma membrane, PM), S6K and <t>ERK1/2</t> (cytosol, Cyt). The purified lysosomes are free of contamination from other organelles. (D) Intactness of the lysosome preparation. Fluorescence images of lysosomes isolated from HeLa cells expressing Lyso-2Strep (green), eluted from streptavidin beads with 20 mM biotin and then labeled with LysoTracker (red). Scale bar: 20 μm. (E) Effect of incubation time and bead abundance. The same amount of PNS (100 µl) was incubated with 30 µl or 60 µl of 1 µm diameter streptavidin beads (Thermo Fisher Scientific) for different time periods as indicated. The relative recovery efficiency of lysosomes was determined by comparing the density of LAMP2 to that of PNS on the same western blots.
    Kallikrein 13 Klk13, supplied by Sino Biological, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/kallikrein 13 klk13/product/Sino Biological
    Average 90 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    kallikrein 13 klk13 - by Bioz Stars, 2021-05
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    Image Search Results


    CB1 activation promoted bone marrow-derived monocyte/macrophage (BMM) polarization toward M1 through G(α) i/o , RhoA, and ERK1/2. BMMs were pre-treated with pertussis toxin (20 ng/mL), Y27632 (10 µmol/L), SB203580 (10 µmol/L), PD98059 (10 µmol/L), compound C (10 µmol/L) for 1 h, and followed by 1 µmol/L ACEA treatment for another 6 h. CD86 mRNA expression (A) and M1 other gene signatures (B–D) were evaluated by RT-qPCR. * P

    Journal: Frontiers in Immunology

    Article Title: Cannabinoid Receptor 1 Participates in Liver Inflammation by Promoting M1 Macrophage Polarization via RhoA/NF-κB p65 and ERK1/2 Pathways, Respectively, in Mouse Liver Fibrogenesis

    doi: 10.3389/fimmu.2017.01214

    Figure Lengend Snippet: CB1 activation promoted bone marrow-derived monocyte/macrophage (BMM) polarization toward M1 through G(α) i/o , RhoA, and ERK1/2. BMMs were pre-treated with pertussis toxin (20 ng/mL), Y27632 (10 µmol/L), SB203580 (10 µmol/L), PD98059 (10 µmol/L), compound C (10 µmol/L) for 1 h, and followed by 1 µmol/L ACEA treatment for another 6 h. CD86 mRNA expression (A) and M1 other gene signatures (B–D) were evaluated by RT-qPCR. * P

    Article Snippet: ACEA (special CB1 agonists), AM281 (CB1 antagonist), AM630 (CB2 antagonist), SB203580 (p38 inhibitor), PD98059 [extracellular signal-regulated kinase (ERK) inhibitor], and compound C [adenosine monophosphate-activated protein kinase (AMPK) inhibitor] were from TOCRIS/R & D (Minneapolis, MN, USA).

    Techniques: Activation Assay, Derivative Assay, Expressing, Quantitative RT-PCR

    ACEA activated G(α) i/o -coupled CB1 and then enlarged GTP-bound Rho and extracellular signal-regulated kinase (ERK) signal, finally promoting bone marrow-derived monocyte/macrophage (BMM) polarization toward M1. In vitro total and active Rho proteins (A) , total ERK1/2 and phosphor-ERK1/2 (B) expression and NF-kB p65 nuclear translocation (C) was measured by western blot. * P

    Journal: Frontiers in Immunology

    Article Title: Cannabinoid Receptor 1 Participates in Liver Inflammation by Promoting M1 Macrophage Polarization via RhoA/NF-κB p65 and ERK1/2 Pathways, Respectively, in Mouse Liver Fibrogenesis

    doi: 10.3389/fimmu.2017.01214

    Figure Lengend Snippet: ACEA activated G(α) i/o -coupled CB1 and then enlarged GTP-bound Rho and extracellular signal-regulated kinase (ERK) signal, finally promoting bone marrow-derived monocyte/macrophage (BMM) polarization toward M1. In vitro total and active Rho proteins (A) , total ERK1/2 and phosphor-ERK1/2 (B) expression and NF-kB p65 nuclear translocation (C) was measured by western blot. * P

    Article Snippet: ACEA (special CB1 agonists), AM281 (CB1 antagonist), AM630 (CB2 antagonist), SB203580 (p38 inhibitor), PD98059 [extracellular signal-regulated kinase (ERK) inhibitor], and compound C [adenosine monophosphate-activated protein kinase (AMPK) inhibitor] were from TOCRIS/R & D (Minneapolis, MN, USA).

    Techniques: Derivative Assay, In Vitro, Expressing, Translocation Assay, Western Blot

    Activation of CB1 promotes M1 marker expressions in bone marrow-derived monocytes/macrophages (BMMs). BMMs were exposed to ACEA for different times. The mRNA levels of M1 markers were measured (A) . BMMs pretreated with 10 µmol/L AM281 or CB1 RNA interference was exposed to ACEA for 5 h. M1 markers were measured by RT-qPCR (B,C) , western blot (E,F) , and CBA (D) . * P

    Journal: Frontiers in Immunology

    Article Title: Cannabinoid Receptor 1 Participates in Liver Inflammation by Promoting M1 Macrophage Polarization via RhoA/NF-κB p65 and ERK1/2 Pathways, Respectively, in Mouse Liver Fibrogenesis

    doi: 10.3389/fimmu.2017.01214

    Figure Lengend Snippet: Activation of CB1 promotes M1 marker expressions in bone marrow-derived monocytes/macrophages (BMMs). BMMs were exposed to ACEA for different times. The mRNA levels of M1 markers were measured (A) . BMMs pretreated with 10 µmol/L AM281 or CB1 RNA interference was exposed to ACEA for 5 h. M1 markers were measured by RT-qPCR (B,C) , western blot (E,F) , and CBA (D) . * P

    Article Snippet: ACEA (special CB1 agonists), AM281 (CB1 antagonist), AM630 (CB2 antagonist), SB203580 (p38 inhibitor), PD98059 [extracellular signal-regulated kinase (ERK) inhibitor], and compound C [adenosine monophosphate-activated protein kinase (AMPK) inhibitor] were from TOCRIS/R & D (Minneapolis, MN, USA).

    Techniques: Activation Assay, Marker, Derivative Assay, Quantitative RT-PCR, Western Blot, Crocin Bleaching Assay

    Determination of factors critical to lysosome purification. (A) Effect of bead sizes using beads from Creative Diagnostics. The same amount of PNS was incubated with streptavidin beads of different diameters as indicated for 5 min. The volume of the beads for each size was adjusted to have the same binding capacity. Lysosome abundance in the purified products was determined by western blotting for LAMP2, showing that 1 µm diameter beads are the most efficient. (B) Comparison of the effect of bead sizes on lysosomal purification efficiency using beads from Thermo Fisher Scientific. Western blot analyses of LAMP2 from PNS and products of purification of Lyso-2Strep using 25 µm and 1 µm streptavidin beads. (C) Purity of the lysosome preparation. Western blot analyses of PNS and products after purification with Lyso-2Strep using 1 µm streptavidin beads. Intracellular organelle markers used were: LAMP1 and LAMP2 (lysosomes, Lyso), SDHA and TOM20 (mitochondria, Mito), SLC27A2 and catalase (peroxisomes, Pex), PDI and SERCA (endoplasmic reticulum, ER), Golgin-97 and GOLM1 (Golgi), ATP1A1 and PMCA2 (plasma membrane, PM), S6K and ERK1/2 (cytosol, Cyt). The purified lysosomes are free of contamination from other organelles. (D) Intactness of the lysosome preparation. Fluorescence images of lysosomes isolated from HeLa cells expressing Lyso-2Strep (green), eluted from streptavidin beads with 20 mM biotin and then labeled with LysoTracker (red). Scale bar: 20 μm. (E) Effect of incubation time and bead abundance. The same amount of PNS (100 µl) was incubated with 30 µl or 60 µl of 1 µm diameter streptavidin beads (Thermo Fisher Scientific) for different time periods as indicated. The relative recovery efficiency of lysosomes was determined by comparing the density of LAMP2 to that of PNS on the same western blots.

    Journal: Journal of Cell Science

    Article Title: Rapid affinity purification of intracellular organelles using a twin strep tag

    doi: 10.1242/jcs.235390

    Figure Lengend Snippet: Determination of factors critical to lysosome purification. (A) Effect of bead sizes using beads from Creative Diagnostics. The same amount of PNS was incubated with streptavidin beads of different diameters as indicated for 5 min. The volume of the beads for each size was adjusted to have the same binding capacity. Lysosome abundance in the purified products was determined by western blotting for LAMP2, showing that 1 µm diameter beads are the most efficient. (B) Comparison of the effect of bead sizes on lysosomal purification efficiency using beads from Thermo Fisher Scientific. Western blot analyses of LAMP2 from PNS and products of purification of Lyso-2Strep using 25 µm and 1 µm streptavidin beads. (C) Purity of the lysosome preparation. Western blot analyses of PNS and products after purification with Lyso-2Strep using 1 µm streptavidin beads. Intracellular organelle markers used were: LAMP1 and LAMP2 (lysosomes, Lyso), SDHA and TOM20 (mitochondria, Mito), SLC27A2 and catalase (peroxisomes, Pex), PDI and SERCA (endoplasmic reticulum, ER), Golgin-97 and GOLM1 (Golgi), ATP1A1 and PMCA2 (plasma membrane, PM), S6K and ERK1/2 (cytosol, Cyt). The purified lysosomes are free of contamination from other organelles. (D) Intactness of the lysosome preparation. Fluorescence images of lysosomes isolated from HeLa cells expressing Lyso-2Strep (green), eluted from streptavidin beads with 20 mM biotin and then labeled with LysoTracker (red). Scale bar: 20 μm. (E) Effect of incubation time and bead abundance. The same amount of PNS (100 µl) was incubated with 30 µl or 60 µl of 1 µm diameter streptavidin beads (Thermo Fisher Scientific) for different time periods as indicated. The relative recovery efficiency of lysosomes was determined by comparing the density of LAMP2 to that of PNS on the same western blots.

    Article Snippet: Antibodies for S6K (cat. #9202, 1:1000), SDHA (cat. #5839, 1:1000), catalase (cat. #12980, 1:1000), PDI (cat. #3501, 1:1000), Golgin-97 (cat. #13192, 1:1000) and ERK1/2 (cat. #4696, 1:1000) were from Cell Signaling Technology (Danvers, MA); antibody for SERCA (cat. #SC-271669, 1:1000) was from Santa Cruz Biotechnology (Dallas, TX); antibody for LAMP1 (cat. #L1418, 1:2500) was from Sigma-Aldrich (St Louis, MO); antibodies for PMCA2 (cat. #19678-1-AP, 1:1000), ATP1A1 (cat. #14418-1-AP, 1:1000), GOLM1 (15126-1-AP, 1:1000), SLC27A2 (14048-1-AP, 1:1000) and TOM20 (cat. #11802-1-AP, 1:10,000) were from ProteinTech (Rosemont, IL); and antibody for LAMP2 (cat. #9840-01, 1:2500) was from the Developmental Studies Hybridoma Bank (Iowa City, IA).

    Techniques: Purification, Incubation, Binding Assay, Western Blot, Fluorescence, Isolation, Expressing, Labeling