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  • 86
    Arch Pharmalabs arch pharm res 2014
    Arch Pharm Res 2014, supplied by Arch Pharmalabs, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/arch pharm res 2014/product/Arch Pharmalabs
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    arch pharm res 2014 - by Bioz Stars, 2021-05
    86/100 stars
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    91
    Sino Biological recombinant gp120
    Activation of <t>gp120-primed</t> DC reduced the expression of Bcl2 and activated Akt, and the induction of cell apoptosis is ASK1-dependent. ( A ) moDC treated with immune-complex gp120 ADA were exposed to CL40L Tf or mock Tf, or LPS, TNFα, IL-1β for 3 d, and cellular proteins of moDC (recovered from coculture) were extracted for Western blotting analysis. MoDC treated by anti-His Ab (used to cross-link recombinant gp120 ADA ) were used as a control (control DC). *p-ASK1 represent results from untreated moDC (lane 1) and DC treated as indicated but no gp120 pulsing (lanes 2–6), whereas #p-ASK-1 represents results from pre-treatment of anti-DC-SIGN mAbs. Data are representative of 3 experiments. ( B ) moDC were transfected with siRNA against human ASK1 or control siRNA (scrambled) before pulse with immune-complex gp120 and subsequent exposure to CD40L Tf, LPS, TNFα or IL-1β. Data are expressed as mean ± SD from 3 experiments. **p
    Recombinant Gp120, supplied by Sino Biological, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/recombinant gp120/product/Sino Biological
    Average 91 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    recombinant gp120 - by Bioz Stars, 2021-05
    91/100 stars
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    88
    Sino Biological enzyme linked immunosorbent assay
    Activation of <t>gp120-primed</t> DC reduced the expression of Bcl2 and activated Akt, and the induction of cell apoptosis is ASK1-dependent. ( A ) moDC treated with immune-complex gp120 ADA were exposed to CL40L Tf or mock Tf, or LPS, TNFα, IL-1β for 3 d, and cellular proteins of moDC (recovered from coculture) were extracted for Western blotting analysis. MoDC treated by anti-His Ab (used to cross-link recombinant gp120 ADA ) were used as a control (control DC). *p-ASK1 represent results from untreated moDC (lane 1) and DC treated as indicated but no gp120 pulsing (lanes 2–6), whereas #p-ASK-1 represents results from pre-treatment of anti-DC-SIGN mAbs. Data are representative of 3 experiments. ( B ) moDC were transfected with siRNA against human ASK1 or control siRNA (scrambled) before pulse with immune-complex gp120 and subsequent exposure to CD40L Tf, LPS, TNFα or IL-1β. Data are expressed as mean ± SD from 3 experiments. **p
    Enzyme Linked Immunosorbent Assay, supplied by Sino Biological, used in various techniques. Bioz Stars score: 88/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/enzyme linked immunosorbent assay/product/Sino Biological
    Average 88 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    enzyme linked immunosorbent assay - by Bioz Stars, 2021-05
    88/100 stars
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    99
    Cell Signaling Technology Inc rabbit anti erk1 2
    Activation of <t>gp120-primed</t> DC reduced the expression of Bcl2 and activated Akt, and the induction of cell apoptosis is ASK1-dependent. ( A ) moDC treated with immune-complex gp120 ADA were exposed to CL40L Tf or mock Tf, or LPS, TNFα, IL-1β for 3 d, and cellular proteins of moDC (recovered from coculture) were extracted for Western blotting analysis. MoDC treated by anti-His Ab (used to cross-link recombinant gp120 ADA ) were used as a control (control DC). *p-ASK1 represent results from untreated moDC (lane 1) and DC treated as indicated but no gp120 pulsing (lanes 2–6), whereas #p-ASK-1 represents results from pre-treatment of anti-DC-SIGN mAbs. Data are representative of 3 experiments. ( B ) moDC were transfected with siRNA against human ASK1 or control siRNA (scrambled) before pulse with immune-complex gp120 and subsequent exposure to CD40L Tf, LPS, TNFα or IL-1β. Data are expressed as mean ± SD from 3 experiments. **p
    Rabbit Anti Erk1 2, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/rabbit anti erk1 2/product/Cell Signaling Technology Inc
    Average 99 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    rabbit anti erk1 2 - by Bioz Stars, 2021-05
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    Image Search Results


    Activation of gp120-primed DC reduced the expression of Bcl2 and activated Akt, and the induction of cell apoptosis is ASK1-dependent. ( A ) moDC treated with immune-complex gp120 ADA were exposed to CL40L Tf or mock Tf, or LPS, TNFα, IL-1β for 3 d, and cellular proteins of moDC (recovered from coculture) were extracted for Western blotting analysis. MoDC treated by anti-His Ab (used to cross-link recombinant gp120 ADA ) were used as a control (control DC). *p-ASK1 represent results from untreated moDC (lane 1) and DC treated as indicated but no gp120 pulsing (lanes 2–6), whereas #p-ASK-1 represents results from pre-treatment of anti-DC-SIGN mAbs. Data are representative of 3 experiments. ( B ) moDC were transfected with siRNA against human ASK1 or control siRNA (scrambled) before pulse with immune-complex gp120 and subsequent exposure to CD40L Tf, LPS, TNFα or IL-1β. Data are expressed as mean ± SD from 3 experiments. **p

    Journal: PLoS Pathogens

    Article Title: Binding of HIV-1 gp120 to DC-SIGN Promotes ASK-1-Dependent Activation-Induced Apoptosis of Human Dendritic Cells

    doi: 10.1371/journal.ppat.1003100

    Figure Lengend Snippet: Activation of gp120-primed DC reduced the expression of Bcl2 and activated Akt, and the induction of cell apoptosis is ASK1-dependent. ( A ) moDC treated with immune-complex gp120 ADA were exposed to CL40L Tf or mock Tf, or LPS, TNFα, IL-1β for 3 d, and cellular proteins of moDC (recovered from coculture) were extracted for Western blotting analysis. MoDC treated by anti-His Ab (used to cross-link recombinant gp120 ADA ) were used as a control (control DC). *p-ASK1 represent results from untreated moDC (lane 1) and DC treated as indicated but no gp120 pulsing (lanes 2–6), whereas #p-ASK-1 represents results from pre-treatment of anti-DC-SIGN mAbs. Data are representative of 3 experiments. ( B ) moDC were transfected with siRNA against human ASK1 or control siRNA (scrambled) before pulse with immune-complex gp120 and subsequent exposure to CD40L Tf, LPS, TNFα or IL-1β. Data are expressed as mean ± SD from 3 experiments. **p

    Article Snippet: Figure S2 EndoH treatment of recombinant gp120 abolished its binding to moDC. (A ) Recombinant gp120ADA supernatant was treated overnight with 25 KU of EndoH/ml as described (Hong PWP et al, J Virol 2002;76:12855–12865), and the treated and the untreated gp120ADA supernatant were subjected to Western blot assay by polyclonal rabbit anti-gp120 Ab (Sino Biological Inc).

    Techniques: Activation Assay, Expressing, Western Blot, Recombinant, Transfection

    Cross-linked gp120 sensitizes DC through DC-SIGN and MCLRs for CD40L-mediated apoptosis. ( A , B ) moDC were pretreated with anti-DC-SIGN mAbs or isotype control Ab before pulse with cross-linked gp120 ADA and co-culture for 3 d with autologous activated CD4 T cells, and subsequently subjected to cell viability assay. Data are representative of 3 experiments and are expressed as mean ± SD from 3 experiments in B . ( C, D ) moDC were treated with cross-linked recombinant gp120 ADA with or without pre-treatment by soluble ICAM-3-Fc chimeric protein, anti-CD4 plus anti-CCR5 mAbs, or anti-DC-SIGN mAbs. Cells were subsequently co-cultured with mock- or CD40L-transfected (CD40L Tf) cells for 3 d. Data are representative of 7 experiments in panel C and are expressed as mean ± SD (n = 7) in D ; ***p

    Journal: PLoS Pathogens

    Article Title: Binding of HIV-1 gp120 to DC-SIGN Promotes ASK-1-Dependent Activation-Induced Apoptosis of Human Dendritic Cells

    doi: 10.1371/journal.ppat.1003100

    Figure Lengend Snippet: Cross-linked gp120 sensitizes DC through DC-SIGN and MCLRs for CD40L-mediated apoptosis. ( A , B ) moDC were pretreated with anti-DC-SIGN mAbs or isotype control Ab before pulse with cross-linked gp120 ADA and co-culture for 3 d with autologous activated CD4 T cells, and subsequently subjected to cell viability assay. Data are representative of 3 experiments and are expressed as mean ± SD from 3 experiments in B . ( C, D ) moDC were treated with cross-linked recombinant gp120 ADA with or without pre-treatment by soluble ICAM-3-Fc chimeric protein, anti-CD4 plus anti-CCR5 mAbs, or anti-DC-SIGN mAbs. Cells were subsequently co-cultured with mock- or CD40L-transfected (CD40L Tf) cells for 3 d. Data are representative of 7 experiments in panel C and are expressed as mean ± SD (n = 7) in D ; ***p

    Article Snippet: Figure S2 EndoH treatment of recombinant gp120 abolished its binding to moDC. (A ) Recombinant gp120ADA supernatant was treated overnight with 25 KU of EndoH/ml as described (Hong PWP et al, J Virol 2002;76:12855–12865), and the treated and the untreated gp120ADA supernatant were subjected to Western blot assay by polyclonal rabbit anti-gp120 Ab (Sino Biological Inc).

    Techniques: Co-Culture Assay, Viability Assay, Recombinant, Cell Culture, Transfection

    Sera from HIV-1(+) individuals can sensitize moDC for DC-SIGN dependent CD40L-mediated apoptosis. ( A ) moDC were treated with HIV(+) serum before or after immunoprecipitation (IP) with anti-gp120 mAbs, or with or without anti-DC-SIGN or isotype control mAbs, and subsequently co-cultured with autologous activated CD4 T cells. After 3 d, cells were harvested and subjected to TUNEL assays. Cell death was assessed as the percentage of cells expressing terminal deoxynucleotidyl transferase (TdT). DC pulsed with HIV(+) serum without coculture with activated CD4 T cells (top panel) were also used as a control. Data are representative of 4 experiments. ( B ) MoDCs were treated with anti-DC-SIGN mAbs, isotype control Ab, or anti-CD40L mAb before pulse with HIV serum (before or after immunoprecipitation of gp120) and cocultured with activated CD4 T cells. Data are expressed as mean ± SD (n = 4); *p

    Journal: PLoS Pathogens

    Article Title: Binding of HIV-1 gp120 to DC-SIGN Promotes ASK-1-Dependent Activation-Induced Apoptosis of Human Dendritic Cells

    doi: 10.1371/journal.ppat.1003100

    Figure Lengend Snippet: Sera from HIV-1(+) individuals can sensitize moDC for DC-SIGN dependent CD40L-mediated apoptosis. ( A ) moDC were treated with HIV(+) serum before or after immunoprecipitation (IP) with anti-gp120 mAbs, or with or without anti-DC-SIGN or isotype control mAbs, and subsequently co-cultured with autologous activated CD4 T cells. After 3 d, cells were harvested and subjected to TUNEL assays. Cell death was assessed as the percentage of cells expressing terminal deoxynucleotidyl transferase (TdT). DC pulsed with HIV(+) serum without coculture with activated CD4 T cells (top panel) were also used as a control. Data are representative of 4 experiments. ( B ) MoDCs were treated with anti-DC-SIGN mAbs, isotype control Ab, or anti-CD40L mAb before pulse with HIV serum (before or after immunoprecipitation of gp120) and cocultured with activated CD4 T cells. Data are expressed as mean ± SD (n = 4); *p

    Article Snippet: Figure S2 EndoH treatment of recombinant gp120 abolished its binding to moDC. (A ) Recombinant gp120ADA supernatant was treated overnight with 25 KU of EndoH/ml as described (Hong PWP et al, J Virol 2002;76:12855–12865), and the treated and the untreated gp120ADA supernatant were subjected to Western blot assay by polyclonal rabbit anti-gp120 Ab (Sino Biological Inc).

    Techniques: Immunoprecipitation, Cell Culture, TUNEL Assay, Expressing

    Cross-linked recombinant gp120 sensitizes moDC for CD40L-mediated apoptosis after co-culture with activated CD4 T cells. ( A ) moDC were treated for 24 h with anti-His mAb alone (control DC, left panels) or with 25 nM gp120 ADA cross-linked with anti-His mAb (gp120-DC, right panels), and co-cultured with autologous activated (upper panels) or naïve (lower panels) CD4 T cells for 3 d. The moDC ( Fig. S1 ) were analyzed for Annexin V (AV) and propidium iodide (PI) expression to assess the extent of apoptosis, as manifested by the percentage of the AV-positive [AV(+)] cells. Data are representative of 5 experiments. ( B ) Apoptosis of moDC was analyzed after treatment with different concentrations of cross-linked recombinant gp120 ADA or gp120 HXBc2 and co-culture with activated CD4 T cells for 3 d. DC treated with monomeric gp120 (not cross-linked with anti-His or anti-FLAG mAb) were used as a control. Data represent mean ± SD from 5 experiments; **p

    Journal: PLoS Pathogens

    Article Title: Binding of HIV-1 gp120 to DC-SIGN Promotes ASK-1-Dependent Activation-Induced Apoptosis of Human Dendritic Cells

    doi: 10.1371/journal.ppat.1003100

    Figure Lengend Snippet: Cross-linked recombinant gp120 sensitizes moDC for CD40L-mediated apoptosis after co-culture with activated CD4 T cells. ( A ) moDC were treated for 24 h with anti-His mAb alone (control DC, left panels) or with 25 nM gp120 ADA cross-linked with anti-His mAb (gp120-DC, right panels), and co-cultured with autologous activated (upper panels) or naïve (lower panels) CD4 T cells for 3 d. The moDC ( Fig. S1 ) were analyzed for Annexin V (AV) and propidium iodide (PI) expression to assess the extent of apoptosis, as manifested by the percentage of the AV-positive [AV(+)] cells. Data are representative of 5 experiments. ( B ) Apoptosis of moDC was analyzed after treatment with different concentrations of cross-linked recombinant gp120 ADA or gp120 HXBc2 and co-culture with activated CD4 T cells for 3 d. DC treated with monomeric gp120 (not cross-linked with anti-His or anti-FLAG mAb) were used as a control. Data represent mean ± SD from 5 experiments; **p

    Article Snippet: Figure S2 EndoH treatment of recombinant gp120 abolished its binding to moDC. (A ) Recombinant gp120ADA supernatant was treated overnight with 25 KU of EndoH/ml as described (Hong PWP et al, J Virol 2002;76:12855–12865), and the treated and the untreated gp120ADA supernatant were subjected to Western blot assay by polyclonal rabbit anti-gp120 Ab (Sino Biological Inc).

    Techniques: Recombinant, Co-Culture Assay, Cell Culture, Expressing

    Cross-linked recombinant gp120 or HIV(+) serum sensitizes moDC for apoptosis after activation by LPS, TNF-α or IL-1β, and DC-SIGN(+) cells in HIV(+) blood are pre-sensitized for LPS/TNFα/IL-1β-induced apoptosis. ( A,B ) moDC were treated with gp120 ADA in the presence of isotype control or anti-DC-SIGN Abs and subsequently cultured in the absence or presence of 100 ng/ml LPS for 3 d. Data are representative of 5 experiments in A and are expressed as mean ± SD in B ; **p

    Journal: PLoS Pathogens

    Article Title: Binding of HIV-1 gp120 to DC-SIGN Promotes ASK-1-Dependent Activation-Induced Apoptosis of Human Dendritic Cells

    doi: 10.1371/journal.ppat.1003100

    Figure Lengend Snippet: Cross-linked recombinant gp120 or HIV(+) serum sensitizes moDC for apoptosis after activation by LPS, TNF-α or IL-1β, and DC-SIGN(+) cells in HIV(+) blood are pre-sensitized for LPS/TNFα/IL-1β-induced apoptosis. ( A,B ) moDC were treated with gp120 ADA in the presence of isotype control or anti-DC-SIGN Abs and subsequently cultured in the absence or presence of 100 ng/ml LPS for 3 d. Data are representative of 5 experiments in A and are expressed as mean ± SD in B ; **p

    Article Snippet: Figure S2 EndoH treatment of recombinant gp120 abolished its binding to moDC. (A ) Recombinant gp120ADA supernatant was treated overnight with 25 KU of EndoH/ml as described (Hong PWP et al, J Virol 2002;76:12855–12865), and the treated and the untreated gp120ADA supernatant were subjected to Western blot assay by polyclonal rabbit anti-gp120 Ab (Sino Biological Inc).

    Techniques: Recombinant, Activation Assay, Cell Culture

    Freshly-isolated DC-SIGN(+) blood DC underwent DC-SIGN-dependent CD40L-mediated apoptosis and DC-SIGN(+) cells from HIV-1-infected individuals are pre-sensitized for CD40L-mediated apoptosis. ( A ) PBMCs from normal HIV(−) individuals were labelled with anti-CD14 plus either isotype control (left panel) or anti-DC-SIGN (right panel) mAbs and analysed by flow cytometry for cell isolation. Data are representative of 4 experiments. ( B ) Purified CD14(+)DC-SIGN(+) cells were treated with anti-His mAb alone (Control) or anti-His cross-linked recombinant gp120 ADA , in the absence or presence of anti-DC-SIGN mAbs, and subsequently co-cultured with CD40L Tf for 3 d. The non-adherent DC were then harvested and subjected to cell viability assay. Data are representative of 4 experiments. ( C, D ) freshly isolated DC-SIGN(+) cells from HIV(+) and HIV(−) blood were cocultured with mock Tf or CD40L Tf for 3 days and subjected to cell viability assay. Data are representative of 4 experiments in C and expressed as mean ± SD in D . ***p

    Journal: PLoS Pathogens

    Article Title: Binding of HIV-1 gp120 to DC-SIGN Promotes ASK-1-Dependent Activation-Induced Apoptosis of Human Dendritic Cells

    doi: 10.1371/journal.ppat.1003100

    Figure Lengend Snippet: Freshly-isolated DC-SIGN(+) blood DC underwent DC-SIGN-dependent CD40L-mediated apoptosis and DC-SIGN(+) cells from HIV-1-infected individuals are pre-sensitized for CD40L-mediated apoptosis. ( A ) PBMCs from normal HIV(−) individuals were labelled with anti-CD14 plus either isotype control (left panel) or anti-DC-SIGN (right panel) mAbs and analysed by flow cytometry for cell isolation. Data are representative of 4 experiments. ( B ) Purified CD14(+)DC-SIGN(+) cells were treated with anti-His mAb alone (Control) or anti-His cross-linked recombinant gp120 ADA , in the absence or presence of anti-DC-SIGN mAbs, and subsequently co-cultured with CD40L Tf for 3 d. The non-adherent DC were then harvested and subjected to cell viability assay. Data are representative of 4 experiments. ( C, D ) freshly isolated DC-SIGN(+) cells from HIV(+) and HIV(−) blood were cocultured with mock Tf or CD40L Tf for 3 days and subjected to cell viability assay. Data are representative of 4 experiments in C and expressed as mean ± SD in D . ***p

    Article Snippet: Figure S2 EndoH treatment of recombinant gp120 abolished its binding to moDC. (A ) Recombinant gp120ADA supernatant was treated overnight with 25 KU of EndoH/ml as described (Hong PWP et al, J Virol 2002;76:12855–12865), and the treated and the untreated gp120ADA supernatant were subjected to Western blot assay by polyclonal rabbit anti-gp120 Ab (Sino Biological Inc).

    Techniques: Isolation, Infection, Flow Cytometry, Cell Isolation, Purification, Recombinant, Cell Culture, Viability Assay