Journal: International Journal of Molecular Sciences
Article Title: Asperuloside and Asperulosidic Acid Exert an Anti-Inflammatory Effect via Suppression of the NF-κB and MAPK Signaling Pathways in LPS-Induced RAW 264.7 Macrophages
Figure Lengend Snippet: Effects of ASP and ASPA on nuclear factor-kappaB alpaha (IκBα), p38, extracellular signal-regulated protein kinases 1/2 (Erk1/2), and stress-activated protein kinase (SAPK)/c-Jun N-terminal kinase (JNK) phosphorylation. RAW 264.7 cells were treated with ASP and ASPA (40, 80, and 160 μg/mL) for 1 h and then induced with LPS (50 ng/mL) for 24 h. The protein was analyzed by Western blot. The quantitative evaluation of protein bands by densitometry is shown. ( A ) The phosphorylation levels of IκBα, p38, Erk1/2, and SAPK/JNK in ASP treatment groups. ( B ) The phosphorylation levels of IκBα, p38, Erk1/2, and SAPK/JNK in ASPA treatment groups. * p
Article Snippet: The antibodies against iNOS, COX-2, IκB-α, p38, and Erk1/2 were obtained from Proteintech Group, Inc. (Chicago, IL, USA).
Techniques: Western Blot