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  • 86
    Cell Signaling Technology Inc p erk1 2
    Protein level transformation is analyzed by Western blot. Notes: The phosphorylation level of JNK and <t>ERK1/2</t> had increased and the non-phosphorylation was invariable in PPP5C downregulation group. The P38 protein level is always consistent with phosphorylation or non-phosphorylation after PPP5C knockdown in the 3 groups, using GAPDH as the loading control. Con: no lentivirus-mediated shRNA; Lv-shCon: lentivirus-mediated non-silencing shRNA; Lv-shPPP5C: lentivirus-mediated non-silencing shPPP5C. Abbreviations: ERK, extracellular signal-regulated protein kinase; GAPDH, glyceraldehyde-3-phosphate dehydrogenase; JNK, c-Jun NH2-terminal kinase; PPP5C, serine/threonine protein phosphatase 5.
    P Erk1 2, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/p erk1 2/product/Cell Signaling Technology Inc
    Average 86 stars, based on 1 article reviews
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    97
    Cell Signaling Technology Inc erk1 2
    NSG3 inhibited PD-L1 expression by suppressing the phosphorylation of <t>Erk1/2.</t> A. PANC-1, BxPC-3 and SW1990 cells were infected with lentivirus vectors expressing control or NSG3-specific shRNAs. After 72 hours postinfection, the cells were harvested for Western blotting analysis. B. PANC-1, BxPC-3 and SW1990 cells were infected with pcDNA3.1 or Flag-NSG3 plasmid. After 72 hours postinfection, the cells were harvested for Western blotting analysis. C. PANC-1, BxPC-3 and SW1990 cells were infected with lentivirus vectors expressing control or NSG3-specific shRNAs. After 48 hours infection, the cells were treated with AG126 (25 uM) for another 24 hours. Then, cells were harvested for Western blotting analysis. D. NSG3 inhibited the phosphorylation level of ERK1/2 to regulate the expression of PD-L1.
    Erk1 2, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/erk1 2/product/Cell Signaling Technology Inc
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    98
    Proteintech rabbit erk1 2 polyclonal
    NSG3 inhibited PD-L1 expression by suppressing the phosphorylation of <t>Erk1/2.</t> A. PANC-1, BxPC-3 and SW1990 cells were infected with lentivirus vectors expressing control or NSG3-specific shRNAs. After 72 hours postinfection, the cells were harvested for Western blotting analysis. B. PANC-1, BxPC-3 and SW1990 cells were infected with pcDNA3.1 or Flag-NSG3 plasmid. After 72 hours postinfection, the cells were harvested for Western blotting analysis. C. PANC-1, BxPC-3 and SW1990 cells were infected with lentivirus vectors expressing control or NSG3-specific shRNAs. After 48 hours infection, the cells were treated with AG126 (25 uM) for another 24 hours. Then, cells were harvested for Western blotting analysis. D. NSG3 inhibited the phosphorylation level of ERK1/2 to regulate the expression of PD-L1.
    Rabbit Erk1 2 Polyclonal, supplied by Proteintech, used in various techniques. Bioz Stars score: 98/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/rabbit erk1 2 polyclonal/product/Proteintech
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    99
    Cell Signaling Technology Inc akt
    Effect of ASC-MVs on the activation level of <t>AKT</t> and <t>ERK</t> signaling pathways. Western blot analysis of the phosphorylation level of AKT and ERK1/2 in cells treated with 20 μg/ml ASC-MVs for the indicated lengths of time. a The ratio of p-AKT/AKT in HUVECs was examined and included on the blots at different time points. b The ratio of p-ERK/ERK in HUVECs was determined. d The ratio of p-ERK/ERK and p-AKT/AKT in HaCAT was determined. e The ratio of p-ERK/ERK and p-AKT/AKT in fibroblasts was determined. Qualification of the ratio of p-ERK/ERK and p-AKT/AKT at different time points. c , f Qualified data shown in a and b . g , h Qualified data shown in d . i , j Qualified data shown in e . N = 3. * p
    Akt, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/akt/product/Cell Signaling Technology Inc
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    Image Search Results


    Protein level transformation is analyzed by Western blot. Notes: The phosphorylation level of JNK and ERK1/2 had increased and the non-phosphorylation was invariable in PPP5C downregulation group. The P38 protein level is always consistent with phosphorylation or non-phosphorylation after PPP5C knockdown in the 3 groups, using GAPDH as the loading control. Con: no lentivirus-mediated shRNA; Lv-shCon: lentivirus-mediated non-silencing shRNA; Lv-shPPP5C: lentivirus-mediated non-silencing shPPP5C. Abbreviations: ERK, extracellular signal-regulated protein kinase; GAPDH, glyceraldehyde-3-phosphate dehydrogenase; JNK, c-Jun NH2-terminal kinase; PPP5C, serine/threonine protein phosphatase 5.

    Journal: OncoTargets and therapy

    Article Title: PPP5C promotes cell proliferation and survival in human prostate cancer by regulating of the JNK and ERK1/2 phosphorylation

    doi: 10.2147/OTT.S161280

    Figure Lengend Snippet: Protein level transformation is analyzed by Western blot. Notes: The phosphorylation level of JNK and ERK1/2 had increased and the non-phosphorylation was invariable in PPP5C downregulation group. The P38 protein level is always consistent with phosphorylation or non-phosphorylation after PPP5C knockdown in the 3 groups, using GAPDH as the loading control. Con: no lentivirus-mediated shRNA; Lv-shCon: lentivirus-mediated non-silencing shRNA; Lv-shPPP5C: lentivirus-mediated non-silencing shPPP5C. Abbreviations: ERK, extracellular signal-regulated protein kinase; GAPDH, glyceraldehyde-3-phosphate dehydrogenase; JNK, c-Jun NH2-terminal kinase; PPP5C, serine/threonine protein phosphatase 5.

    Article Snippet: Following, the PVDF membranes were blocked in the 40–50 mL tris-buffered saline with Tween 20 (TBST) with 5% milk for 1 hour and incubated with the following primary antibodies overnight at 4°C: glyceraldehyde-3-phosphate dehydrogenase (#:10494-1-AP, Proteintech); PPP5C (#:11715-1-AP, Proteintech), JNK (#:9252, Cell Signaling, Beverly, MA, USA), P-JNK (#:4668, Cell Sig-naling), P38 (#:14064-1-AP, Proteintech), P-P38 (#:11581, Cell Signaling), ERK1/2 (#:11257-1-AP, Proteintech), and P-ERK1/2 (#:4370, Cell Signaling).

    Techniques: Transformation Assay, Western Blot, shRNA

    NSG3 inhibited PD-L1 expression by suppressing the phosphorylation of Erk1/2. A. PANC-1, BxPC-3 and SW1990 cells were infected with lentivirus vectors expressing control or NSG3-specific shRNAs. After 72 hours postinfection, the cells were harvested for Western blotting analysis. B. PANC-1, BxPC-3 and SW1990 cells were infected with pcDNA3.1 or Flag-NSG3 plasmid. After 72 hours postinfection, the cells were harvested for Western blotting analysis. C. PANC-1, BxPC-3 and SW1990 cells were infected with lentivirus vectors expressing control or NSG3-specific shRNAs. After 48 hours infection, the cells were treated with AG126 (25 uM) for another 24 hours. Then, cells were harvested for Western blotting analysis. D. NSG3 inhibited the phosphorylation level of ERK1/2 to regulate the expression of PD-L1.

    Journal: American Journal of Cancer Research

    Article Title: Decreased NSG3 enhances PD-L1 expression by Erk1/2 pathway to promote pancreatic cancer progress

    doi:

    Figure Lengend Snippet: NSG3 inhibited PD-L1 expression by suppressing the phosphorylation of Erk1/2. A. PANC-1, BxPC-3 and SW1990 cells were infected with lentivirus vectors expressing control or NSG3-specific shRNAs. After 72 hours postinfection, the cells were harvested for Western blotting analysis. B. PANC-1, BxPC-3 and SW1990 cells were infected with pcDNA3.1 or Flag-NSG3 plasmid. After 72 hours postinfection, the cells were harvested for Western blotting analysis. C. PANC-1, BxPC-3 and SW1990 cells were infected with lentivirus vectors expressing control or NSG3-specific shRNAs. After 48 hours infection, the cells were treated with AG126 (25 uM) for another 24 hours. Then, cells were harvested for Western blotting analysis. D. NSG3 inhibited the phosphorylation level of ERK1/2 to regulate the expression of PD-L1.

    Article Snippet: Antibodies and reagentsThe antibodies used included NSG3 (absin, abs138459, working concentration 1:1000), PD-L1 (Proteitech, 17952-1-AP, working concentration 1:1000), Erk1/2 (Cell Signaling Technology, 4695, working concentration 1:1000), pErk1/2-Thr202/Tyr204 (Cell Signaling Technology, 9101, working concentration 1:1000), and GAPDH (Proteitech, 10494-1-AP, working concentration 1:2000).

    Techniques: Expressing, Infection, Western Blot, Plasmid Preparation

    Effect of ASC-MVs on the activation level of AKT and ERK signaling pathways. Western blot analysis of the phosphorylation level of AKT and ERK1/2 in cells treated with 20 μg/ml ASC-MVs for the indicated lengths of time. a The ratio of p-AKT/AKT in HUVECs was examined and included on the blots at different time points. b The ratio of p-ERK/ERK in HUVECs was determined. d The ratio of p-ERK/ERK and p-AKT/AKT in HaCAT was determined. e The ratio of p-ERK/ERK and p-AKT/AKT in fibroblasts was determined. Qualification of the ratio of p-ERK/ERK and p-AKT/AKT at different time points. c , f Qualified data shown in a and b . g , h Qualified data shown in d . i , j Qualified data shown in e . N = 3. * p

    Journal: Stem Cell Research & Therapy

    Article Title: Microvesicles from human adipose stem cells promote wound healing by optimizing cellular functions via AKT and ERK signaling pathways

    doi: 10.1186/s13287-019-1152-x

    Figure Lengend Snippet: Effect of ASC-MVs on the activation level of AKT and ERK signaling pathways. Western blot analysis of the phosphorylation level of AKT and ERK1/2 in cells treated with 20 μg/ml ASC-MVs for the indicated lengths of time. a The ratio of p-AKT/AKT in HUVECs was examined and included on the blots at different time points. b The ratio of p-ERK/ERK in HUVECs was determined. d The ratio of p-ERK/ERK and p-AKT/AKT in HaCAT was determined. e The ratio of p-ERK/ERK and p-AKT/AKT in fibroblasts was determined. Qualification of the ratio of p-ERK/ERK and p-AKT/AKT at different time points. c , f Qualified data shown in a and b . g , h Qualified data shown in d . i , j Qualified data shown in e . N = 3. * p

    Article Snippet: Briefly, equal amount of total protein (20–40 μg) was separated by SDS-PAGE (Beyotime Biotechnology, Shanghai, China), transferred into the PVDF membrane (Millipore, USA), and then incubated overnight with primary antibodies specific for cyclin D1 (#A11022, ABclonal, China), VEGFR2 (#A5609, ABclonal, China), fibronectin (#A12932, ABclonal, China), α-SMA (#A7248, ABclonal, China), VEGFA (#ab52917, Abcam), cyclin A2 (#ab181591, Abcam), PDGFR (#ab69506, Abcam), collagen I (#ab138492, Abcam), collagen III (#ab184993, Abcam), elastin (#ab213720, Abcam), GAPDH (#10494-1-AP, Proteintech, China), ERK (#4695, Cell Signaling Technology), p-ERK (#4370, Cell Signaling Technology), AKT (#4691, Cell Signaling Technology), and p-AKT (#4060, Cell Signaling Technology).

    Techniques: Activation Assay, Western Blot