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  • 93
    Proteintech antibody against rab5a
    Clinical significance of <t>Rab5a</t> in human OSCC
    Antibody Against Rab5a, supplied by Proteintech, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/antibody against rab5a/product/Proteintech
    Average 93 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    antibody against rab5a - by Bioz Stars, 2021-05
    93/100 stars
      Buy from Supplier

    86
    Cell Signaling Technology Inc anti fate1 antibody
    <t>FATE1</t> supports Ewing sarcoma cell viability. (A) Relative viability levels of the indicated cell lines measured 120 h after transfection with the indicated siRNAs. Bars represent means ( n  = 3) of viability levels relative to siCTRL ± SD. (B) Bright-field images of cells assayed as described in the panel A legend and acquired with a 5× lens objective. (C) At 72 to 96 h after siRNA transfection, WCLs were collected from the indicated cell lines and immunoblotted with the indicated antibodies. (D) (Left) At 48 h after infection with the indicated lentiviral constructs, RNA was collected and used in qPCR for analysis of FATE1 mRNA expression. Bars represent means ( n  = 2) of expression levels of FATE1 relative to shCTRL ± range. (Right) At 48 h after infection with the indicated lentiviral constructs, cells were seeded in soft agar. After 3 weeks, colonies were stained and imaged.
    Anti Fate1 Antibody, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/anti fate1 antibody/product/Cell Signaling Technology Inc
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    anti fate1 antibody - by Bioz Stars, 2021-05
    86/100 stars
      Buy from Supplier

    99
    Santa Cruz Biotechnology vimentin sc 73259
    <t>FATE1</t> supports Ewing sarcoma cell viability. (A) Relative viability levels of the indicated cell lines measured 120 h after transfection with the indicated siRNAs. Bars represent means ( n  = 3) of viability levels relative to siCTRL ± SD. (B) Bright-field images of cells assayed as described in the panel A legend and acquired with a 5× lens objective. (C) At 72 to 96 h after siRNA transfection, WCLs were collected from the indicated cell lines and immunoblotted with the indicated antibodies. (D) (Left) At 48 h after infection with the indicated lentiviral constructs, RNA was collected and used in qPCR for analysis of FATE1 mRNA expression. Bars represent means ( n  = 2) of expression levels of FATE1 relative to shCTRL ± range. (Right) At 48 h after infection with the indicated lentiviral constructs, cells were seeded in soft agar. After 3 weeks, colonies were stained and imaged.
    Vimentin Sc 73259, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/vimentin sc 73259/product/Santa Cruz Biotechnology
    Average 99 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    vimentin sc 73259 - by Bioz Stars, 2021-05
    99/100 stars
      Buy from Supplier

    86
    Cell Signaling Technology Inc p egfr
    <t>FATE1</t> supports Ewing sarcoma cell viability. (A) Relative viability levels of the indicated cell lines measured 120 h after transfection with the indicated siRNAs. Bars represent means ( n  = 3) of viability levels relative to siCTRL ± SD. (B) Bright-field images of cells assayed as described in the panel A legend and acquired with a 5× lens objective. (C) At 72 to 96 h after siRNA transfection, WCLs were collected from the indicated cell lines and immunoblotted with the indicated antibodies. (D) (Left) At 48 h after infection with the indicated lentiviral constructs, RNA was collected and used in qPCR for analysis of FATE1 mRNA expression. Bars represent means ( n  = 2) of expression levels of FATE1 relative to shCTRL ± range. (Right) At 48 h after infection with the indicated lentiviral constructs, cells were seeded in soft agar. After 3 weeks, colonies were stained and imaged.
    P Egfr, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/p egfr/product/Cell Signaling Technology Inc
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    p egfr - by Bioz Stars, 2021-05
    86/100 stars
      Buy from Supplier

    Image Search Results


    Clinical significance of Rab5a in human OSCC

    Journal: Molecular Medicine Reports

    Article Title: Rab5a is overexpressed in oral cancer and promotes invasion through ERK/MMP signaling

    doi: 10.3892/mmr.2017.7214

    Figure Lengend Snippet: Clinical significance of Rab5a in human OSCC

    Article Snippet: Protein samples on SDS-PAGE were transferred to PVDF membranes (Millipore Corp., Billerica, MA, USA) and incubated overnight at 4°C with primary antibody against Rab5a (11947–1-AP) (1:1,000; ProteinTech Group, Inc., Chicago, IL, USA), p-extracellular signal-regulated kinase (ERK) (no. 4370), ERK (no. 9102), MMP-2 (no. 87809), cyclin D1 (no. 2978), cyclin E (no. 4129), cyclin A (no. 4656), cyclin B (no. 4135), E-cadherin (no. 14472), p-FAK (no. 8556), p-EGFR (no. 3777), p-MEK (no. 3958), MEK (no. 4694) (1:1,000; Cell Signaling Technology, Inc., Boston, MA, USA), Snail (sc-10432), Vimentin (sc-73259) (1:500 dilution; Santa Cruz Biotechnology, Inc., Santa Cruz, CA, USA) and GAPDH (sc-47724) (1:1,000; Santa Cruz Biotechnology, Inc.).

    Techniques:

    Rab5a promotes proliferation and invasion in OSCC cell lines. (A) MTT assay showed that Rab5a overexpression upregulated cell proliferation rate in FaDu cell line while Rab5a siRNA downregulated cell proliferation rate in Detroit 562 cell line. (B) Colony

    Journal: Molecular Medicine Reports

    Article Title: Rab5a is overexpressed in oral cancer and promotes invasion through ERK/MMP signaling

    doi: 10.3892/mmr.2017.7214

    Figure Lengend Snippet: Rab5a promotes proliferation and invasion in OSCC cell lines. (A) MTT assay showed that Rab5a overexpression upregulated cell proliferation rate in FaDu cell line while Rab5a siRNA downregulated cell proliferation rate in Detroit 562 cell line. (B) Colony

    Article Snippet: Protein samples on SDS-PAGE were transferred to PVDF membranes (Millipore Corp., Billerica, MA, USA) and incubated overnight at 4°C with primary antibody against Rab5a (11947–1-AP) (1:1,000; ProteinTech Group, Inc., Chicago, IL, USA), p-extracellular signal-regulated kinase (ERK) (no. 4370), ERK (no. 9102), MMP-2 (no. 87809), cyclin D1 (no. 2978), cyclin E (no. 4129), cyclin A (no. 4656), cyclin B (no. 4135), E-cadherin (no. 14472), p-FAK (no. 8556), p-EGFR (no. 3777), p-MEK (no. 3958), MEK (no. 4694) (1:1,000; Cell Signaling Technology, Inc., Boston, MA, USA), Snail (sc-10432), Vimentin (sc-73259) (1:500 dilution; Santa Cruz Biotechnology, Inc., Santa Cruz, CA, USA) and GAPDH (sc-47724) (1:1,000; Santa Cruz Biotechnology, Inc.).

    Techniques: MTT Assay, Over Expression

    Rab5a regulates ERK signaling and epithelial-mesenchymal transition. (A) Western blot showed that Rab5a transfection upregulated cyclin D1, cyclin E, cyclin A, MMP-2, p-ERK, Snail, Vimentin expression and downregulated E-cadherin. Rab5a depletion downregulated

    Journal: Molecular Medicine Reports

    Article Title: Rab5a is overexpressed in oral cancer and promotes invasion through ERK/MMP signaling

    doi: 10.3892/mmr.2017.7214

    Figure Lengend Snippet: Rab5a regulates ERK signaling and epithelial-mesenchymal transition. (A) Western blot showed that Rab5a transfection upregulated cyclin D1, cyclin E, cyclin A, MMP-2, p-ERK, Snail, Vimentin expression and downregulated E-cadherin. Rab5a depletion downregulated

    Article Snippet: Protein samples on SDS-PAGE were transferred to PVDF membranes (Millipore Corp., Billerica, MA, USA) and incubated overnight at 4°C with primary antibody against Rab5a (11947–1-AP) (1:1,000; ProteinTech Group, Inc., Chicago, IL, USA), p-extracellular signal-regulated kinase (ERK) (no. 4370), ERK (no. 9102), MMP-2 (no. 87809), cyclin D1 (no. 2978), cyclin E (no. 4129), cyclin A (no. 4656), cyclin B (no. 4135), E-cadherin (no. 14472), p-FAK (no. 8556), p-EGFR (no. 3777), p-MEK (no. 3958), MEK (no. 4694) (1:1,000; Cell Signaling Technology, Inc., Boston, MA, USA), Snail (sc-10432), Vimentin (sc-73259) (1:500 dilution; Santa Cruz Biotechnology, Inc., Santa Cruz, CA, USA) and GAPDH (sc-47724) (1:1,000; Santa Cruz Biotechnology, Inc.).

    Techniques: Western Blot, Transfection, Expressing

    Rab5a regulates cell cycle and migration. (A) Rab5a facilitated cell cycle transition by upregulating S phase percentage while Rab5a depletion downregulated S phase percentage. (B) Wound healing assay showed that Rab5a overexpression upregulated cell

    Journal: Molecular Medicine Reports

    Article Title: Rab5a is overexpressed in oral cancer and promotes invasion through ERK/MMP signaling

    doi: 10.3892/mmr.2017.7214

    Figure Lengend Snippet: Rab5a regulates cell cycle and migration. (A) Rab5a facilitated cell cycle transition by upregulating S phase percentage while Rab5a depletion downregulated S phase percentage. (B) Wound healing assay showed that Rab5a overexpression upregulated cell

    Article Snippet: Protein samples on SDS-PAGE were transferred to PVDF membranes (Millipore Corp., Billerica, MA, USA) and incubated overnight at 4°C with primary antibody against Rab5a (11947–1-AP) (1:1,000; ProteinTech Group, Inc., Chicago, IL, USA), p-extracellular signal-regulated kinase (ERK) (no. 4370), ERK (no. 9102), MMP-2 (no. 87809), cyclin D1 (no. 2978), cyclin E (no. 4129), cyclin A (no. 4656), cyclin B (no. 4135), E-cadherin (no. 14472), p-FAK (no. 8556), p-EGFR (no. 3777), p-MEK (no. 3958), MEK (no. 4694) (1:1,000; Cell Signaling Technology, Inc., Boston, MA, USA), Snail (sc-10432), Vimentin (sc-73259) (1:500 dilution; Santa Cruz Biotechnology, Inc., Santa Cruz, CA, USA) and GAPDH (sc-47724) (1:1,000; Santa Cruz Biotechnology, Inc.).

    Techniques: Migration, Wound Healing Assay, Over Expression

    Transfection efficiency of Rab5a plasmid and siRNA. (A) Western blot showed that high Rab5a expression in Detroit 562 and low in FaDu cells. (B) Western blot and polymerase chain reaction analysis showed that Rab5a transfection in FaDu cells increased

    Journal: Molecular Medicine Reports

    Article Title: Rab5a is overexpressed in oral cancer and promotes invasion through ERK/MMP signaling

    doi: 10.3892/mmr.2017.7214

    Figure Lengend Snippet: Transfection efficiency of Rab5a plasmid and siRNA. (A) Western blot showed that high Rab5a expression in Detroit 562 and low in FaDu cells. (B) Western blot and polymerase chain reaction analysis showed that Rab5a transfection in FaDu cells increased

    Article Snippet: Protein samples on SDS-PAGE were transferred to PVDF membranes (Millipore Corp., Billerica, MA, USA) and incubated overnight at 4°C with primary antibody against Rab5a (11947–1-AP) (1:1,000; ProteinTech Group, Inc., Chicago, IL, USA), p-extracellular signal-regulated kinase (ERK) (no. 4370), ERK (no. 9102), MMP-2 (no. 87809), cyclin D1 (no. 2978), cyclin E (no. 4129), cyclin A (no. 4656), cyclin B (no. 4135), E-cadherin (no. 14472), p-FAK (no. 8556), p-EGFR (no. 3777), p-MEK (no. 3958), MEK (no. 4694) (1:1,000; Cell Signaling Technology, Inc., Boston, MA, USA), Snail (sc-10432), Vimentin (sc-73259) (1:500 dilution; Santa Cruz Biotechnology, Inc., Santa Cruz, CA, USA) and GAPDH (sc-47724) (1:1,000; Santa Cruz Biotechnology, Inc.).

    Techniques: Transfection, Plasmid Preparation, Western Blot, Expressing, Polymerase Chain Reaction

    Expression of Rab5a protein in OSCC tissues. (A) Negative Rab5a expression in normal oral squamous mucosa. Scale bar: 50 µm. (B) Weak Rab5a expression in a case of stage 1, grade 1 OSCC. (C) Positive cytoplamic Rab5a expression in a case of stage

    Journal: Molecular Medicine Reports

    Article Title: Rab5a is overexpressed in oral cancer and promotes invasion through ERK/MMP signaling

    doi: 10.3892/mmr.2017.7214

    Figure Lengend Snippet: Expression of Rab5a protein in OSCC tissues. (A) Negative Rab5a expression in normal oral squamous mucosa. Scale bar: 50 µm. (B) Weak Rab5a expression in a case of stage 1, grade 1 OSCC. (C) Positive cytoplamic Rab5a expression in a case of stage

    Article Snippet: Protein samples on SDS-PAGE were transferred to PVDF membranes (Millipore Corp., Billerica, MA, USA) and incubated overnight at 4°C with primary antibody against Rab5a (11947–1-AP) (1:1,000; ProteinTech Group, Inc., Chicago, IL, USA), p-extracellular signal-regulated kinase (ERK) (no. 4370), ERK (no. 9102), MMP-2 (no. 87809), cyclin D1 (no. 2978), cyclin E (no. 4129), cyclin A (no. 4656), cyclin B (no. 4135), E-cadherin (no. 14472), p-FAK (no. 8556), p-EGFR (no. 3777), p-MEK (no. 3958), MEK (no. 4694) (1:1,000; Cell Signaling Technology, Inc., Boston, MA, USA), Snail (sc-10432), Vimentin (sc-73259) (1:500 dilution; Santa Cruz Biotechnology, Inc., Santa Cruz, CA, USA) and GAPDH (sc-47724) (1:1,000; Santa Cruz Biotechnology, Inc.).

    Techniques: Expressing

    FATE1 supports Ewing sarcoma cell viability. (A) Relative viability levels of the indicated cell lines measured 120 h after transfection with the indicated siRNAs. Bars represent means ( n  = 3) of viability levels relative to siCTRL ± SD. (B) Bright-field images of cells assayed as described in the panel A legend and acquired with a 5× lens objective. (C) At 72 to 96 h after siRNA transfection, WCLs were collected from the indicated cell lines and immunoblotted with the indicated antibodies. (D) (Left) At 48 h after infection with the indicated lentiviral constructs, RNA was collected and used in qPCR for analysis of FATE1 mRNA expression. Bars represent means ( n  = 2) of expression levels of FATE1 relative to shCTRL ± range. (Right) At 48 h after infection with the indicated lentiviral constructs, cells were seeded in soft agar. After 3 weeks, colonies were stained and imaged.

    Journal: Molecular and Cellular Biology

    Article Title: EWSR1-FLI1 Activation of the Cancer/Testis Antigen FATE1 Promotes Ewing Sarcoma Survival

    doi: 10.1128/MCB.00138-19

    Figure Lengend Snippet: FATE1 supports Ewing sarcoma cell viability. (A) Relative viability levels of the indicated cell lines measured 120 h after transfection with the indicated siRNAs. Bars represent means ( n  = 3) of viability levels relative to siCTRL ± SD. (B) Bright-field images of cells assayed as described in the panel A legend and acquired with a 5× lens objective. (C) At 72 to 96 h after siRNA transfection, WCLs were collected from the indicated cell lines and immunoblotted with the indicated antibodies. (D) (Left) At 48 h after infection with the indicated lentiviral constructs, RNA was collected and used in qPCR for analysis of FATE1 mRNA expression. Bars represent means ( n  = 2) of expression levels of FATE1 relative to shCTRL ± range. (Right) At 48 h after infection with the indicated lentiviral constructs, cells were seeded in soft agar. After 3 weeks, colonies were stained and imaged.

    Article Snippet: Antibodies used for immunoblotting were as follows: from Santa Cruz Biotechnology, GAPDH (glyceraldehyde-3-phosphate dehydrogenase) (sc-51907; 1:1,000), hemagglutinin (HA) (sc-805; 1:500), actin (sc-8432 [1:2,500] and sc-47778 [1:5,000]), c-Myc (9E10 [sc-40; 1:2,500] and A-14 [sc-789; 1:1,000]), and extracellular signal-regulated kinase 1 and 2 (ERK1/2) (sc-93; 1:1,000); from Sigma-Aldrich, FATE1 (HPA034604; 1:1,000), RNF183 (SAB2106627; 1:1,000), and HA (clone 3F10; 1:1,000); from Cell Signaling Technology, cleaved caspase-3 (9661; 1:500), PARP (9532; 1:1,000), BNIP3L (12396; 1:1,000), and V5 (13202; 1:2,500); from Abcam, FATE1 (ab111486; 1:1,000); from BD Biosciences, FLI1 (554266; 1:500) and BNIP3 (10433; 1:1,000).

    Techniques: Transfection, Infection, Construct, Real-time Polymerase Chain Reaction, Expressing, Staining

    FATE1 destabilizes BNIP3L. (A and B) At 72 h after siRNA transfection, WCLs were collected from the indicated cell lines and immunoblotted with the indicated antibodies. Bars represent means ( n  = 3) of protein abundance levels relative to siCTRL ± SEM. (C) At 48 h after transfection with the indicated cDNA constructs, WCLs were collected from EWS502 cells and immunoblotted with the indicated antibodies. (D) At 48 h after transfection with the indicated cDNA constructs, WCLs were collected from HeLa cells and immunoblotted with the indicated antibodies. (E) At 72 h after siRNA transfection, WCLs were collected from EWS502 and immunoblotted with the indicated antibodies.

    Journal: Molecular and Cellular Biology

    Article Title: EWSR1-FLI1 Activation of the Cancer/Testis Antigen FATE1 Promotes Ewing Sarcoma Survival

    doi: 10.1128/MCB.00138-19

    Figure Lengend Snippet: FATE1 destabilizes BNIP3L. (A and B) At 72 h after siRNA transfection, WCLs were collected from the indicated cell lines and immunoblotted with the indicated antibodies. Bars represent means ( n  = 3) of protein abundance levels relative to siCTRL ± SEM. (C) At 48 h after transfection with the indicated cDNA constructs, WCLs were collected from EWS502 cells and immunoblotted with the indicated antibodies. (D) At 48 h after transfection with the indicated cDNA constructs, WCLs were collected from HeLa cells and immunoblotted with the indicated antibodies. (E) At 72 h after siRNA transfection, WCLs were collected from EWS502 and immunoblotted with the indicated antibodies.

    Article Snippet: Antibodies used for immunoblotting were as follows: from Santa Cruz Biotechnology, GAPDH (glyceraldehyde-3-phosphate dehydrogenase) (sc-51907; 1:1,000), hemagglutinin (HA) (sc-805; 1:500), actin (sc-8432 [1:2,500] and sc-47778 [1:5,000]), c-Myc (9E10 [sc-40; 1:2,500] and A-14 [sc-789; 1:1,000]), and extracellular signal-regulated kinase 1 and 2 (ERK1/2) (sc-93; 1:1,000); from Sigma-Aldrich, FATE1 (HPA034604; 1:1,000), RNF183 (SAB2106627; 1:1,000), and HA (clone 3F10; 1:1,000); from Cell Signaling Technology, cleaved caspase-3 (9661; 1:500), PARP (9532; 1:1,000), BNIP3L (12396; 1:1,000), and V5 (13202; 1:2,500); from Abcam, FATE1 (ab111486; 1:1,000); from BD Biosciences, FLI1 (554266; 1:500) and BNIP3 (10433; 1:1,000).

    Techniques: Transfection, Construct

    FATE1 and RNF183 collaborate to promote BNIP3L turnover. (A) At 48 h after transfection in HEK293T, cell lysates were immunoprecipitated with c-Myc or V5 antibodies and immunoblotted with the indicated antibodies. (B) At 72 h after transfection with the indicated siRNAs, WCLs were collected from TC-32 cells and immunoblotted with the indicated antibodies. Arrows indicate RNF183 bands. (C) WCLs were collected from the indicated cell lines and immunoblotted for the indicated antibodies. (D) At 48 h after transfection with the indicated cDNA constructs, lysates were collected from HeLa cells and immunoblotted with the indicated antibodies. (E) At 48 h after transfection with the indicated cDNA constructs, lysates were collected from HeLa cells and immunoblotted with the indicated antibodies. (F) At 48 h after transfection in HEK293T cells, protein lysates were incubated with V5 antibodies and immunoblotted with the indicated antibodies. All samples contained pcDNA3-FLAG-Ub. (G) Proposed model for FATE1 contributing to Ewing sarcoma tumorigenesis.

    Journal: Molecular and Cellular Biology

    Article Title: EWSR1-FLI1 Activation of the Cancer/Testis Antigen FATE1 Promotes Ewing Sarcoma Survival

    doi: 10.1128/MCB.00138-19

    Figure Lengend Snippet: FATE1 and RNF183 collaborate to promote BNIP3L turnover. (A) At 48 h after transfection in HEK293T, cell lysates were immunoprecipitated with c-Myc or V5 antibodies and immunoblotted with the indicated antibodies. (B) At 72 h after transfection with the indicated siRNAs, WCLs were collected from TC-32 cells and immunoblotted with the indicated antibodies. Arrows indicate RNF183 bands. (C) WCLs were collected from the indicated cell lines and immunoblotted for the indicated antibodies. (D) At 48 h after transfection with the indicated cDNA constructs, lysates were collected from HeLa cells and immunoblotted with the indicated antibodies. (E) At 48 h after transfection with the indicated cDNA constructs, lysates were collected from HeLa cells and immunoblotted with the indicated antibodies. (F) At 48 h after transfection in HEK293T cells, protein lysates were incubated with V5 antibodies and immunoblotted with the indicated antibodies. All samples contained pcDNA3-FLAG-Ub. (G) Proposed model for FATE1 contributing to Ewing sarcoma tumorigenesis.

    Article Snippet: Antibodies used for immunoblotting were as follows: from Santa Cruz Biotechnology, GAPDH (glyceraldehyde-3-phosphate dehydrogenase) (sc-51907; 1:1,000), hemagglutinin (HA) (sc-805; 1:500), actin (sc-8432 [1:2,500] and sc-47778 [1:5,000]), c-Myc (9E10 [sc-40; 1:2,500] and A-14 [sc-789; 1:1,000]), and extracellular signal-regulated kinase 1 and 2 (ERK1/2) (sc-93; 1:1,000); from Sigma-Aldrich, FATE1 (HPA034604; 1:1,000), RNF183 (SAB2106627; 1:1,000), and HA (clone 3F10; 1:1,000); from Cell Signaling Technology, cleaved caspase-3 (9661; 1:500), PARP (9532; 1:1,000), BNIP3L (12396; 1:1,000), and V5 (13202; 1:2,500); from Abcam, FATE1 (ab111486; 1:1,000); from BD Biosciences, FLI1 (554266; 1:500) and BNIP3 (10433; 1:1,000).

    Techniques: Transfection, Immunoprecipitation, Construct, Incubation