Journal: Oncology Reports
Article Title: Ad-p53 enhances the sensitivity of triple-negative breast cancer MDA-MB-468 cells to the EGFR inhibitor gefitinib
Figure Lengend Snippet: Induction of apoptosis following treatments with 3 μM of gefitinib alone, MOI of 100 of Ad-p53 alone or in combination for 48 h. The apoptosis of MDA-MB-468 cells was detected via Annexin V/FITC using flow cytometry. (A) Percentage of apoptotic cells was obtained from UR and LR panels in each scatter plot for all the treatment groups. (B) Data represent means ± SEM from three independent experiments. Statistical significance was assessed by ANOVA. Tukey’s multiple comparison was applied to compare two subsequent samples. ** P
Article Snippet: Ad-p53 (Gendicine, Shenzhen, China), 1×1012 virus particles (VP), was stored at −20°C; Annexin V-FITC apoptosis kit was purchased from BD Biosciences Pharmingen (San Diego, CA, USA). p53, caspase-9, Akt, phosphorylation of protein kinase B (p-Akt) (S473), extracellular signal-regulated kinase (ERK) and phosphorylated ERK (p-ERK) (Y204) were purchased from ImmunoWay Biotechnology (Grand Island, NY, USA).
Techniques: Multiple Displacement Amplification, Flow Cytometry, Cytometry