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  • 93
    Santa Cruz Biotechnology anti hdac6
    Anti Hdac6, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/anti hdac6/product/Santa Cruz Biotechnology
    Average 93 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    anti hdac6 - by Bioz Stars, 2021-05
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    91
    Sino Biological egfr matched elisa antibody pair set human
    <t>EGFR</t> levels confirmed by western blot analysis and <t>ELISA</t> in CAD patients and control subjects. (A) Western blotting images of plasma EGFR and loading control GAPDH were assayed in CAD patients and controls. (B) Quantification of relative intensity levels of EGFR in CAD patients and controls adjusted for loading control. ImageJ software was used. (C) ELISA-based comparison of mean EGFR levels between the CAD patients and control groups. A significantly higher level of EGFR was observed in the MI and SA groups in comparison with controls. (D) EGFR levels were only significantly different between male MI groups in comparison with male control group. The EGFR levels were significantly different between female MI and SA groups when compared with controls. *P≤0.05, **P≤0.001. EGFR, epidermal growth factor receptor; CAD, coronary artery disease; MI, myocardial infarction; SA, stable angina.
    Egfr Matched Elisa Antibody Pair Set Human, supplied by Sino Biological, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/egfr matched elisa antibody pair set human/product/Sino Biological
    Average 91 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    egfr matched elisa antibody pair set human - by Bioz Stars, 2021-05
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    93
    Santa Cruz Biotechnology anti alix
    <t>EGFR</t> levels confirmed by western blot analysis and <t>ELISA</t> in CAD patients and control subjects. (A) Western blotting images of plasma EGFR and loading control GAPDH were assayed in CAD patients and controls. (B) Quantification of relative intensity levels of EGFR in CAD patients and controls adjusted for loading control. ImageJ software was used. (C) ELISA-based comparison of mean EGFR levels between the CAD patients and control groups. A significantly higher level of EGFR was observed in the MI and SA groups in comparison with controls. (D) EGFR levels were only significantly different between male MI groups in comparison with male control group. The EGFR levels were significantly different between female MI and SA groups when compared with controls. *P≤0.05, **P≤0.001. EGFR, epidermal growth factor receptor; CAD, coronary artery disease; MI, myocardial infarction; SA, stable angina.
    Anti Alix, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/anti alix/product/Santa Cruz Biotechnology
    Average 93 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    anti alix - by Bioz Stars, 2021-05
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    90
    Sino Biological recombinant his tagged hc1inh
    Shake-flask-like cell growth profile and volumetric productivity in a 96-HDW-microplate. ( a – c ) Comparison of cell growth in shake flasks (three flasks) and HDW-microplate (96 wells). CHO-S cells were seeded at 3 × 10 5 cells/mL in shake flasks (25 mL in 125 mL flasks) or in HDWs (250 μL) and VCD and viability were measured daily (day 0–4). ( a ) VCD (solid lines) and viability (dashed lines) versus culture time. Red and black lines show data from shake flasks and HDWs, respectively. VCD values are plotted on a logarithmic scale. ( b ) Coefficient of variation (CV) of VCD measurements in HDWs during the four days of culture. ( c ) Doubling time from day 0–3 in 96 wells calculated by exponential growth equation (least squares fit) in GraphPad Prism (GraphPad Software). Median and interquartile range are depicted and CV is shown above the data points. ( d – g ) Comparison of volumetric productivity in shake flasks and HDWs of hα1AT, hEPO, Rituximab and <t>hC1INH.</t> CHO-S cells were transiently transfected with plasmids encoding the aforementioned proteins and supernatants two days post-transfection were obtained and titers were determined by ELISA (hα1AT, hEPO and hC1INH) and bio-layer interferometry (Rituximab). 16 and 4 separate transfections were performed in HDWs and flasks, respectively. Mean, standard deviation, and percentage difference are shown.
    Recombinant His Tagged Hc1inh, supplied by Sino Biological, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/recombinant his tagged hc1inh/product/Sino Biological
    Average 90 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    recombinant his tagged hc1inh - by Bioz Stars, 2021-05
    90/100 stars
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    Image Search Results


    EGFR levels confirmed by western blot analysis and ELISA in CAD patients and control subjects. (A) Western blotting images of plasma EGFR and loading control GAPDH were assayed in CAD patients and controls. (B) Quantification of relative intensity levels of EGFR in CAD patients and controls adjusted for loading control. ImageJ software was used. (C) ELISA-based comparison of mean EGFR levels between the CAD patients and control groups. A significantly higher level of EGFR was observed in the MI and SA groups in comparison with controls. (D) EGFR levels were only significantly different between male MI groups in comparison with male control group. The EGFR levels were significantly different between female MI and SA groups when compared with controls. *P≤0.05, **P≤0.001. EGFR, epidermal growth factor receptor; CAD, coronary artery disease; MI, myocardial infarction; SA, stable angina.

    Journal: Molecular Medicine Reports

    Article Title: Integrative gene ontology and network analysis of coronary artery disease associated genes suggests potential role of ErbB pathway gene EGFR

    doi: 10.3892/mmr.2018.8393

    Figure Lengend Snippet: EGFR levels confirmed by western blot analysis and ELISA in CAD patients and control subjects. (A) Western blotting images of plasma EGFR and loading control GAPDH were assayed in CAD patients and controls. (B) Quantification of relative intensity levels of EGFR in CAD patients and controls adjusted for loading control. ImageJ software was used. (C) ELISA-based comparison of mean EGFR levels between the CAD patients and control groups. A significantly higher level of EGFR was observed in the MI and SA groups in comparison with controls. (D) EGFR levels were only significantly different between male MI groups in comparison with male control group. The EGFR levels were significantly different between female MI and SA groups when compared with controls. *P≤0.05, **P≤0.001. EGFR, epidermal growth factor receptor; CAD, coronary artery disease; MI, myocardial infarction; SA, stable angina.

    Article Snippet: The plasma level of EGFR was quantified in 342 CAD patients and 342 control samples using an ELISA kit (cat. no. SEK10001; Sino Biological Inc., Beijing, China).

    Techniques: Western Blot, Enzyme-linked Immunosorbent Assay, Software

    An overview of the biomarker prioritization method. The method of biomarker prioritization involves the following: Step 1, selection of CAD related genes from CADgene database; Step 2, gene filtration based on GO and DO; Step 3, the gene set obtained was used for network construction, analysis, identification of hub and pathway enrichment analysis; and, Step 4, biological validation using WB, ELISA and statistical assessment of the biomarker. GO, gene ontology; DO, disease ontology; WB, western blot analysis; CAD, coronary artery disease; EGFR, epidermal growth factor receptor.

    Journal: Molecular Medicine Reports

    Article Title: Integrative gene ontology and network analysis of coronary artery disease associated genes suggests potential role of ErbB pathway gene EGFR

    doi: 10.3892/mmr.2018.8393

    Figure Lengend Snippet: An overview of the biomarker prioritization method. The method of biomarker prioritization involves the following: Step 1, selection of CAD related genes from CADgene database; Step 2, gene filtration based on GO and DO; Step 3, the gene set obtained was used for network construction, analysis, identification of hub and pathway enrichment analysis; and, Step 4, biological validation using WB, ELISA and statistical assessment of the biomarker. GO, gene ontology; DO, disease ontology; WB, western blot analysis; CAD, coronary artery disease; EGFR, epidermal growth factor receptor.

    Article Snippet: The plasma level of EGFR was quantified in 342 CAD patients and 342 control samples using an ELISA kit (cat. no. SEK10001; Sino Biological Inc., Beijing, China).

    Techniques: Biomarker Assay, Selection, Filtration, Western Blot, Enzyme-linked Immunosorbent Assay

    Shake-flask-like cell growth profile and volumetric productivity in a 96-HDW-microplate. ( a – c ) Comparison of cell growth in shake flasks (three flasks) and HDW-microplate (96 wells). CHO-S cells were seeded at 3 × 10 5 cells/mL in shake flasks (25 mL in 125 mL flasks) or in HDWs (250 μL) and VCD and viability were measured daily (day 0–4). ( a ) VCD (solid lines) and viability (dashed lines) versus culture time. Red and black lines show data from shake flasks and HDWs, respectively. VCD values are plotted on a logarithmic scale. ( b ) Coefficient of variation (CV) of VCD measurements in HDWs during the four days of culture. ( c ) Doubling time from day 0–3 in 96 wells calculated by exponential growth equation (least squares fit) in GraphPad Prism (GraphPad Software). Median and interquartile range are depicted and CV is shown above the data points. ( d – g ) Comparison of volumetric productivity in shake flasks and HDWs of hα1AT, hEPO, Rituximab and hC1INH. CHO-S cells were transiently transfected with plasmids encoding the aforementioned proteins and supernatants two days post-transfection were obtained and titers were determined by ELISA (hα1AT, hEPO and hC1INH) and bio-layer interferometry (Rituximab). 16 and 4 separate transfections were performed in HDWs and flasks, respectively. Mean, standard deviation, and percentage difference are shown.

    Journal: Scientific Reports

    Article Title: Versatile microscale screening platform for improving recombinant protein productivity in Chinese hamster ovary cells

    doi: 10.1038/srep18016

    Figure Lengend Snippet: Shake-flask-like cell growth profile and volumetric productivity in a 96-HDW-microplate. ( a – c ) Comparison of cell growth in shake flasks (three flasks) and HDW-microplate (96 wells). CHO-S cells were seeded at 3 × 10 5 cells/mL in shake flasks (25 mL in 125 mL flasks) or in HDWs (250 μL) and VCD and viability were measured daily (day 0–4). ( a ) VCD (solid lines) and viability (dashed lines) versus culture time. Red and black lines show data from shake flasks and HDWs, respectively. VCD values are plotted on a logarithmic scale. ( b ) Coefficient of variation (CV) of VCD measurements in HDWs during the four days of culture. ( c ) Doubling time from day 0–3 in 96 wells calculated by exponential growth equation (least squares fit) in GraphPad Prism (GraphPad Software). Median and interquartile range are depicted and CV is shown above the data points. ( d – g ) Comparison of volumetric productivity in shake flasks and HDWs of hα1AT, hEPO, Rituximab and hC1INH. CHO-S cells were transiently transfected with plasmids encoding the aforementioned proteins and supernatants two days post-transfection were obtained and titers were determined by ELISA (hα1AT, hEPO and hC1INH) and bio-layer interferometry (Rituximab). 16 and 4 separate transfections were performed in HDWs and flasks, respectively. Mean, standard deviation, and percentage difference are shown.

    Article Snippet: Purified recombinant his-tagged hC1INH (#10995-H08H, Sino Biological) was used as standard.

    Techniques: Software, Transfection, Enzyme-linked Immunosorbent Assay, Standard Deviation

    Co-expression of target genes affects split-GFP-based specific and volumetric productivity of hα1AT-S11 and hC1INH-S11. ( a , b ) Plasmid encoding hα1AT N-terminally tagged with S11_M3 was co-transfected with mock plasmid or plasmids encoding target (mouse) genes into CHO-S cells in 96-HDW-microplates. Three days after transfection, supernatants were obtained and subjected to split-GFP product titer assay for determination of relative titer. Split-GFP-based specific productivity ( q p ) and titer are shown. Eight separate transfections were performed in each experiment and experiments were performed twice (n = 2; 16 wells). Mean and standard deviation are depicted. Only statistically significant (p ≤ 0.05) increases in productivity are shown. ( c , d ) As described for panels ( a,b ), with the only difference being use of plasmid encoding hC1INH C-terminally tagged with S11_M3 instead of plasmid encoding hα1AT. ( e , f ) Comparison of split-GFP-based product titer assay and ELISA. hα1AT (mock and Atf4 ) and hC1INH (Mock and Xbp1s ) supernatants described in panels a-d were subjected to ELISA for determination of hα1AT and hC1INH titers. Mean, standard deviation and percentage difference are shown.

    Journal: Scientific Reports

    Article Title: Versatile microscale screening platform for improving recombinant protein productivity in Chinese hamster ovary cells

    doi: 10.1038/srep18016

    Figure Lengend Snippet: Co-expression of target genes affects split-GFP-based specific and volumetric productivity of hα1AT-S11 and hC1INH-S11. ( a , b ) Plasmid encoding hα1AT N-terminally tagged with S11_M3 was co-transfected with mock plasmid or plasmids encoding target (mouse) genes into CHO-S cells in 96-HDW-microplates. Three days after transfection, supernatants were obtained and subjected to split-GFP product titer assay for determination of relative titer. Split-GFP-based specific productivity ( q p ) and titer are shown. Eight separate transfections were performed in each experiment and experiments were performed twice (n = 2; 16 wells). Mean and standard deviation are depicted. Only statistically significant (p ≤ 0.05) increases in productivity are shown. ( c , d ) As described for panels ( a,b ), with the only difference being use of plasmid encoding hC1INH C-terminally tagged with S11_M3 instead of plasmid encoding hα1AT. ( e , f ) Comparison of split-GFP-based product titer assay and ELISA. hα1AT (mock and Atf4 ) and hC1INH (Mock and Xbp1s ) supernatants described in panels a-d were subjected to ELISA for determination of hα1AT and hC1INH titers. Mean, standard deviation and percentage difference are shown.

    Article Snippet: Purified recombinant his-tagged hC1INH (#10995-H08H, Sino Biological) was used as standard.

    Techniques: Expressing, Plasmid Preparation, Transfection, Titer Assay, Standard Deviation, Enzyme-linked Immunosorbent Assay

    Analysis of split-GFP S11-tag variants. ( a – f ) CHO-S cells in 6-well plates were transfected with plasmids encoding hα1AT or hC1INH either S11_M3 or S11_H7-tagged at the N- or C-terminus or untagged. Supernatants two days post-transfection were analyzed. ( a ) α-hα1AT and ( b – d ) α-hC1INH Western blots under reducing conditions. The Western blots shown are representative of three experiments. ( c , d ) Proteins in supernatants were subjected to PNGase F and O -glycosidase (Protein Deglycosylation Mix) treatment under denaturing conditions where indicated. ( e , f ) Supernatants were analyzed by split-GFP product titer assay. Signal-to-noise (S/N) ratio was determined by defining noise as split-GFP complementation signal from mock transfected cells. Mean and standard deviation are depicted (n = 3). ( g ) A dilution series of a synthetic S11_M3 peptide was analyzed by split-GFP product titer assay. The trend line from linear regression and correlation coefficient (R 2 ) are shown. The encircled data point was found to be an outlier in the linear regression analysis. Mean and standard deviation from eight technical replicates are depicted. ( h ) Intra-assay variation of the split-GFP product titer assay was analyzed from the data shown in panel ( e ). The coefficient of variation (CV) at different concentrations of the S11_M3 peptide are shown. The two-headed arrow denotes the defined dynamic linear range with low intra-assay variation (CV

    Journal: Scientific Reports

    Article Title: Versatile microscale screening platform for improving recombinant protein productivity in Chinese hamster ovary cells

    doi: 10.1038/srep18016

    Figure Lengend Snippet: Analysis of split-GFP S11-tag variants. ( a – f ) CHO-S cells in 6-well plates were transfected with plasmids encoding hα1AT or hC1INH either S11_M3 or S11_H7-tagged at the N- or C-terminus or untagged. Supernatants two days post-transfection were analyzed. ( a ) α-hα1AT and ( b – d ) α-hC1INH Western blots under reducing conditions. The Western blots shown are representative of three experiments. ( c , d ) Proteins in supernatants were subjected to PNGase F and O -glycosidase (Protein Deglycosylation Mix) treatment under denaturing conditions where indicated. ( e , f ) Supernatants were analyzed by split-GFP product titer assay. Signal-to-noise (S/N) ratio was determined by defining noise as split-GFP complementation signal from mock transfected cells. Mean and standard deviation are depicted (n = 3). ( g ) A dilution series of a synthetic S11_M3 peptide was analyzed by split-GFP product titer assay. The trend line from linear regression and correlation coefficient (R 2 ) are shown. The encircled data point was found to be an outlier in the linear regression analysis. Mean and standard deviation from eight technical replicates are depicted. ( h ) Intra-assay variation of the split-GFP product titer assay was analyzed from the data shown in panel ( e ). The coefficient of variation (CV) at different concentrations of the S11_M3 peptide are shown. The two-headed arrow denotes the defined dynamic linear range with low intra-assay variation (CV

    Article Snippet: Purified recombinant his-tagged hC1INH (#10995-H08H, Sino Biological) was used as standard.

    Techniques: Transfection, Western Blot, Titer Assay, Standard Deviation, Intra Assay

    Positive effects of target genes on productivity validated on untagged hα1AT and hC1INH. ( a ) Plasmid encoding untagged hα1AT was co-transfected with a mock plasmid or plasmid encoding Atf4 into CHO-S cells in a 96-HDW-microplate. Three days after transfection, supernatants were obtained and hα1AT titer determined by ELISA. Normalized titer and specific productivity ( q p ) are shown. Eight separate transfections were performed in each experiment and experiments were performed twice (n = 2; 16 wells). Mean, standard deviation, and statistically significant (p ≤ 0.05) fold-changes are depicted. ( b ) Plasmid encoding untagged hC1INH was co-transfected with mock plasmid or plasmid encoding Xbp1s into CHO-S cells in 96-HDW-microplate. Three days after transfection, supernatants were obtained and hC1INH titer determined by ELISA. Normalized titer and specific productivity ( q p ) are shown. Eight separate transfections were performed in each experiment and experiments were performed twice (n = 2; 16 wells). Mean, standard deviation, and statistically significant (p ≤ 0.05) fold-changes are depicted.

    Journal: Scientific Reports

    Article Title: Versatile microscale screening platform for improving recombinant protein productivity in Chinese hamster ovary cells

    doi: 10.1038/srep18016

    Figure Lengend Snippet: Positive effects of target genes on productivity validated on untagged hα1AT and hC1INH. ( a ) Plasmid encoding untagged hα1AT was co-transfected with a mock plasmid or plasmid encoding Atf4 into CHO-S cells in a 96-HDW-microplate. Three days after transfection, supernatants were obtained and hα1AT titer determined by ELISA. Normalized titer and specific productivity ( q p ) are shown. Eight separate transfections were performed in each experiment and experiments were performed twice (n = 2; 16 wells). Mean, standard deviation, and statistically significant (p ≤ 0.05) fold-changes are depicted. ( b ) Plasmid encoding untagged hC1INH was co-transfected with mock plasmid or plasmid encoding Xbp1s into CHO-S cells in 96-HDW-microplate. Three days after transfection, supernatants were obtained and hC1INH titer determined by ELISA. Normalized titer and specific productivity ( q p ) are shown. Eight separate transfections were performed in each experiment and experiments were performed twice (n = 2; 16 wells). Mean, standard deviation, and statistically significant (p ≤ 0.05) fold-changes are depicted.

    Article Snippet: Purified recombinant his-tagged hC1INH (#10995-H08H, Sino Biological) was used as standard.

    Techniques: Plasmid Preparation, Transfection, Enzyme-linked Immunosorbent Assay, Standard Deviation