Journal: Molecular Medicine Reports
Article Title: Integrative gene ontology and network analysis of coronary artery disease associated genes suggests potential role of ErbB pathway gene EGFR
Figure Lengend Snippet: An overview of the biomarker prioritization method. The method of biomarker prioritization involves the following: Step 1, selection of CAD related genes from CADgene database; Step 2, gene filtration based on GO and DO; Step 3, the gene set obtained was used for network construction, analysis, identification of hub and pathway enrichment analysis; and, Step 4, biological validation using WB, ELISA and statistical assessment of the biomarker. GO, gene ontology; DO, disease ontology; WB, western blot analysis; CAD, coronary artery disease; EGFR, epidermal growth factor receptor.
Article Snippet: The plasma level of EGFR was quantified in 342 CAD patients and 342 control samples using an ELISA kit (cat. no. SEK10001; Sino Biological Inc., Beijing, China).
Techniques: Biomarker Assay, Selection, Filtration, Western Blot, Enzyme-linked Immunosorbent Assay