Journal: International Journal of Molecular Sciences

Article Title: Choosing the Probe for Single-Molecule Fluorescence Microscopy

doi: 10.3390/ijms232314949

Figure Lengend Snippet: Published comparisons of dyes aspecific interactions. AL: Alexa, AT: Atto, Abb.: Abberior. SE: succinimidyl esters; HY: hydrazide; M: maleimide.

Article Snippet: SNAP-tag technology can be used for both extra- and intracellular labeling: in the first case, using non-cell-permeable substrates such as SNAP-Surface ® (New England Biolabs), for studying, e.g., membrane receptors [ , ]; in the second case, using cell-permeable substrates such as SNAP-Cell ® (New England Biolabs) or Janelia Fluor dyes, a quite recent family of cell-permeable fluorophores for single-molecule imaging [ ] (protocols for the synthesis of SNAP-tag ligands of Janelia Fluor and subsequent labeling can be found in [ ]).

Techniques: Imaging, Labeling, Diffusion-based Assay, Single Particle, Fluorescence, Spectroscopy, Incubation, Transfection