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  • 95
    New England Biolabs 12 tube magnetic separation rack
    12 Tube Magnetic Separation Rack, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 95/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/12 tube magnetic separation rack/product/New England Biolabs
    Average 95 stars, based on 4 article reviews
    Price from $9.99 to $1999.99
    12 tube magnetic separation rack - by Bioz Stars, 2020-08
    95/100 stars
      Buy from Supplier

    99
    Millipore ag490
    Changes of P-Tyr-STAT1, STAT1 and IL-7 expression after STAT1 blockade following KGF treatment, by western blot in LoVo cells (A). Tubulin was used as internal control. Suppressions of P-Tyr-STAT1 and IL-7 expression, but not STAT1, were observed with STAT1 inhibitors including <t>AG490</t> (50 µmol/l) and RPM (50 ng/ml) following KGF (150 ng/ml) treatment. Changes of IL-7 mRNA expression after STAT1 blockade following KGF treatment were detected by quantitative real-time PCR (B), * indicates significant difference between RPM (or AG490) group and control, ** indicates significant difference between RPM (or AG490)+KGF group and control+KGF group, P
    Ag490, supplied by Millipore, used in various techniques. Bioz Stars score: 99/100, based on 1831 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/ag490/product/Millipore
    Average 99 stars, based on 1831 article reviews
    Price from $9.99 to $1999.99
    ag490 - by Bioz Stars, 2020-08
    99/100 stars
      Buy from Supplier

    92
    Covaris 12 tube magnetic separation rack
    Changes of P-Tyr-STAT1, STAT1 and IL-7 expression after STAT1 blockade following KGF treatment, by western blot in LoVo cells (A). Tubulin was used as internal control. Suppressions of P-Tyr-STAT1 and IL-7 expression, but not STAT1, were observed with STAT1 inhibitors including <t>AG490</t> (50 µmol/l) and RPM (50 ng/ml) following KGF (150 ng/ml) treatment. Changes of IL-7 mRNA expression after STAT1 blockade following KGF treatment were detected by quantitative real-time PCR (B), * indicates significant difference between RPM (or AG490) group and control, ** indicates significant difference between RPM (or AG490)+KGF group and control+KGF group, P
    12 Tube Magnetic Separation Rack, supplied by Covaris, used in various techniques. Bioz Stars score: 92/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/12 tube magnetic separation rack/product/Covaris
    Average 92 stars, based on 3 article reviews
    Price from $9.99 to $1999.99
    12 tube magnetic separation rack - by Bioz Stars, 2020-08
    92/100 stars
      Buy from Supplier

    92
    USA Scientific Inc 12 tube magnetic separation rack
    Changes of P-Tyr-STAT1, STAT1 and IL-7 expression after STAT1 blockade following KGF treatment, by western blot in LoVo cells (A). Tubulin was used as internal control. Suppressions of P-Tyr-STAT1 and IL-7 expression, but not STAT1, were observed with STAT1 inhibitors including <t>AG490</t> (50 µmol/l) and RPM (50 ng/ml) following KGF (150 ng/ml) treatment. Changes of IL-7 mRNA expression after STAT1 blockade following KGF treatment were detected by quantitative real-time PCR (B), * indicates significant difference between RPM (or AG490) group and control, ** indicates significant difference between RPM (or AG490)+KGF group and control+KGF group, P
    12 Tube Magnetic Separation Rack, supplied by USA Scientific Inc, used in various techniques. Bioz Stars score: 92/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/12 tube magnetic separation rack/product/USA Scientific Inc
    Average 92 stars, based on 3 article reviews
    Price from $9.99 to $1999.99
    12 tube magnetic separation rack - by Bioz Stars, 2020-08
    92/100 stars
      Buy from Supplier

    Image Search Results


    Changes of P-Tyr-STAT1, STAT1 and IL-7 expression after STAT1 blockade following KGF treatment, by western blot in LoVo cells (A). Tubulin was used as internal control. Suppressions of P-Tyr-STAT1 and IL-7 expression, but not STAT1, were observed with STAT1 inhibitors including AG490 (50 µmol/l) and RPM (50 ng/ml) following KGF (150 ng/ml) treatment. Changes of IL-7 mRNA expression after STAT1 blockade following KGF treatment were detected by quantitative real-time PCR (B), * indicates significant difference between RPM (or AG490) group and control, ** indicates significant difference between RPM (or AG490)+KGF group and control+KGF group, P

    Journal: PLoS ONE

    Article Title: Up-Regulation of Intestinal Epithelial Cell Derived IL-7 Expression by Keratinocyte Growth Factor through STAT1/IRF-1, IRF-2 Pathway

    doi: 10.1371/journal.pone.0058647

    Figure Lengend Snippet: Changes of P-Tyr-STAT1, STAT1 and IL-7 expression after STAT1 blockade following KGF treatment, by western blot in LoVo cells (A). Tubulin was used as internal control. Suppressions of P-Tyr-STAT1 and IL-7 expression, but not STAT1, were observed with STAT1 inhibitors including AG490 (50 µmol/l) and RPM (50 ng/ml) following KGF (150 ng/ml) treatment. Changes of IL-7 mRNA expression after STAT1 blockade following KGF treatment were detected by quantitative real-time PCR (B), * indicates significant difference between RPM (or AG490) group and control, ** indicates significant difference between RPM (or AG490)+KGF group and control+KGF group, P

    Article Snippet: Two of the groups were treated with either RPM (50 ng/ml, catalogue no. 37094; Sigma), or AG490 (50 µmol/l, catalogue no. S1509; Sigma) and one group of cells was left untreated as a control.

    Techniques: Expressing, Western Blot, Real-time Polymerase Chain Reaction

    TLC analysis of the TPL of Irish organic farmed salmon fillets and the biological activity of each PL subclass towards PAF-induced platelet aggregation in hPRP. ( A ): Typical profile of the PL separation of salmon fillet on preparative TLC (Silica G). The elution system used for the separation of the TPL was chloroform:methanol:water 65:35:6 ( v / v / v ). PL: Polar lipids; E: mixture of standard PL from egg; Lyso-PC: lyso-phosphatidylcholine; SM: sphingomyelin; PC: phosphatidylcholine; Lyso-PE: lyso-phosphatidylethanolamine; PE: phosphatidylethanolamine; CL: cardiolipin; TLC; thin-layer chromatography. ( B ): Biological activities of the PL subclasses of the TLC bands towards PAF-induced platelet aggregation in hPRP. The results reflect the inhibitory strength of each lipid sample and are expressed as mean values of IC 50 (μg of lipids in the aggregometer cuvette that causes 50% inhibition of PAF-induced aggregation of hPRP); The low IC 50 value indicates strong inhibition of PAF-induced aggregation of hPRP; Lipid fractions of TLC bands 1 and 4 (corresponding to Lyso-PC and Lyso-PE) did not exhibit these bioactivities. hPRP: human platelet-rich plasma; PAF: platelet-activating factor; PL: polar lipids; TPL: total polar lipids.

    Journal: Marine Drugs

    Article Title: Structural Elucidation of Irish Organic Farmed Salmon (Salmo salar) Polar Lipids with Antithrombotic Activities

    doi: 10.3390/md16060176

    Figure Lengend Snippet: TLC analysis of the TPL of Irish organic farmed salmon fillets and the biological activity of each PL subclass towards PAF-induced platelet aggregation in hPRP. ( A ): Typical profile of the PL separation of salmon fillet on preparative TLC (Silica G). The elution system used for the separation of the TPL was chloroform:methanol:water 65:35:6 ( v / v / v ). PL: Polar lipids; E: mixture of standard PL from egg; Lyso-PC: lyso-phosphatidylcholine; SM: sphingomyelin; PC: phosphatidylcholine; Lyso-PE: lyso-phosphatidylethanolamine; PE: phosphatidylethanolamine; CL: cardiolipin; TLC; thin-layer chromatography. ( B ): Biological activities of the PL subclasses of the TLC bands towards PAF-induced platelet aggregation in hPRP. The results reflect the inhibitory strength of each lipid sample and are expressed as mean values of IC 50 (μg of lipids in the aggregometer cuvette that causes 50% inhibition of PAF-induced aggregation of hPRP); The low IC 50 value indicates strong inhibition of PAF-induced aggregation of hPRP; Lipid fractions of TLC bands 1 and 4 (corresponding to Lyso-PC and Lyso-PE) did not exhibit these bioactivities. hPRP: human platelet-rich plasma; PAF: platelet-activating factor; PL: polar lipids; TPL: total polar lipids.

    Article Snippet: The TLC analysis of the salmon TPL was performed as previously described for other fish species using an egg phospholipid standard (Sigma Aldrich, Wicklow, Ireland) [ ].

    Techniques: Thin Layer Chromatography, Activity Assay, Inhibition

    Lipid Analyses of the Parental Line dw15-1 and pgd1 Mutant under N-Replete and N-Deficient Conditions. (A) Relative abundance of major polar lipids in chloroplasts of the PL dw15-1 and the pgd1 mutant under N-replete and N-deficient conditions. Results are the average of five biological replicates (independent cultures) with error bars indicating standard deviations ( n = 5). Asterisks indicate significant differences between the pgd1 mutant and the PL dw15-1 by paired-sample Student’s t test (*P ≤ 0.05 and **P ≤ 0.01). CP, chloroplast; +N, N replete; N48, N deprivation for 48 h. (B) Fatty acid composition of MGDG in the PL dw15-1 and pgd1 chloroplast before and after N deprivation. Fatty acids are shown as the number of carbons: number of double bonds. Positions of double bonds are indicated from the carboxyl end (Δ). *P ≤ 0.05 and **P ≤ 0.01; n = 5. (C) TLC to separate digestion product of MGDG by RaLIP, a lipase that acts specifically on the sn -1 position of glycerolipids from R. arrhizus . MGDG was isolated from total chloroplast lipids by TLC and then treated with RaLIP at room temperature for 2 h. The hydrolysates free fatty acid and lysoMGDG were purified by TLC for GC-FID analysis. Con, uncut MGDG control; FFA, free fatty acid. (D) and (E) Combination analyses of the acyl chains of MGDG at the sn -1 (D) and sn -2 positions (E) . *P ≤ 0.05 and **P ≤ 0.01; n = 3.

    Journal: The Plant Cell

    Article Title: Galactoglycerolipid Lipase PGD1 Is Involved in Thylakoid Membrane Remodeling in Response to Adverse Environmental Conditions in Chlamydomonas

    doi: 10.1105/tpc.17.00446

    Figure Lengend Snippet: Lipid Analyses of the Parental Line dw15-1 and pgd1 Mutant under N-Replete and N-Deficient Conditions. (A) Relative abundance of major polar lipids in chloroplasts of the PL dw15-1 and the pgd1 mutant under N-replete and N-deficient conditions. Results are the average of five biological replicates (independent cultures) with error bars indicating standard deviations ( n = 5). Asterisks indicate significant differences between the pgd1 mutant and the PL dw15-1 by paired-sample Student’s t test (*P ≤ 0.05 and **P ≤ 0.01). CP, chloroplast; +N, N replete; N48, N deprivation for 48 h. (B) Fatty acid composition of MGDG in the PL dw15-1 and pgd1 chloroplast before and after N deprivation. Fatty acids are shown as the number of carbons: number of double bonds. Positions of double bonds are indicated from the carboxyl end (Δ). *P ≤ 0.05 and **P ≤ 0.01; n = 5. (C) TLC to separate digestion product of MGDG by RaLIP, a lipase that acts specifically on the sn -1 position of glycerolipids from R. arrhizus . MGDG was isolated from total chloroplast lipids by TLC and then treated with RaLIP at room temperature for 2 h. The hydrolysates free fatty acid and lysoMGDG were purified by TLC for GC-FID analysis. Con, uncut MGDG control; FFA, free fatty acid. (D) and (E) Combination analyses of the acyl chains of MGDG at the sn -1 (D) and sn -2 positions (E) . *P ≤ 0.05 and **P ≤ 0.01; n = 3.

    Article Snippet: MGDG was extracted using the TLC method (Silica G60; EMD Millipore).

    Techniques: Mutagenesis, Thin Layer Chromatography, Isolation, Purification