Journal: PLoS ONE
Article Title: Up-Regulation of Intestinal Epithelial Cell Derived IL-7 Expression by Keratinocyte Growth Factor through STAT1/IRF-1, IRF-2 Pathway
Figure Lengend Snippet: Changes of P-Tyr-STAT1, STAT1 and IL-7 expression after STAT1 blockade following KGF treatment, by western blot in LoVo cells (A). Tubulin was used as internal control. Suppressions of P-Tyr-STAT1 and IL-7 expression, but not STAT1, were observed with STAT1 inhibitors including AG490 (50 µmol/l) and RPM (50 ng/ml) following KGF (150 ng/ml) treatment. Changes of IL-7 mRNA expression after STAT1 blockade following KGF treatment were detected by quantitative real-time PCR (B), * indicates significant difference between RPM (or AG490) group and control, ** indicates significant difference between RPM (or AG490)+KGF group and control+KGF group, P
Article Snippet: Two of the groups were treated with either RPM (50 ng/ml, catalogue no. 37094; Sigma), or AG490 (50 µmol/l, catalogue no. S1509; Sigma) and one group of cells was left untreated as a control.
Techniques: Expressing, Western Blot, Real-time Polymerase Chain Reaction