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Image Search Results

Journal: Nature Communications
Article Title: c-Met activation leads to the establishment of a TGFβ-receptor regulatory network in bladder cancer progression
doi: 10.1038/s41467-019-12241-2
Figure Lengend Snippet: c-Met and TGFβ signalling in HGF-mediated EMT of NBT-II carcinoma cells. a Bar graph displays ratio of phosphorylated proteins at 2 h post HGF compared with control. b Western blot analysis of NBT-II cells treated with escalating doses of HGF at various concentrations for 2 h. Lysates are probed with indicated antibodies. c Western blot analysis of NBT-II cells treated with 5 ng/ml of HGF. Lysates were collected at denoted time points and probed with indicated antibodies. d Western blot analysis of NBT-II cells treated with 5 ng/ml of HGF in the absence or presence of c-MET inhibitor JNJ38877605. Lysates were collected at denoted time points and probed with indicated antibodies. e Western blot analysis of nuclear (n) and cytoplasmic (c) fractions isolated from NBT-II cells treated with HGF for 2 h with or without TβRI inhibitor (A83-01, 8 μM). Lysates were collected at denoted time points and probed with indicated antibodies. f Bar chart displays ratio of pSMAD2 band intensity in nuclear (n) fractions compared with total SMAD2 (nuclear + cytoplasmic) fractions isolated from NBT-II cells treated with HGF for 2 h with or without TβRI inhibitor (A83-01, 8 μM). Bars represent mean ± SD of three independent experiments. A two-tailed Student’s t test compares the treated cell populations, ** P
Article Snippet: The following antibodies were used; anti-E-cadherin (BD), anti-FLAG and anti-α-tubulin (Sigma), anti-HA (Y11, Santa Cruz biotech), anti-Myc (9E10 or A14, Santa Cruz Biotech), anti-phospho-SMAD2 (S465/467, Cell Signaling), anti-Laminin-b1 (Abcam), anti-SMAD2 (L16D3, Cell Signaling), anti-β-actin (Sigma), anti-TGF-β receptor I (V-22, Santa Cruz Biotech), anti-phospho-tyrosine (clone 4G10, Millipore, 05–321), anti-Phospho-Src (Tyr 416, 2101 S, Cell Signaling), anti-c-Src (B-12, Santa Cruz Biotech), anti-phospho-ERK (P-p44/42 MAPK T202/Y204, 9101, Cell Signaling), anti-Total ERK (p44/42 MAPK, 9102, Cell Signaling), anti-p38 (Cell Signaling), p-c-MET Tyr1234/1235 (Cell Signaling) and
Techniques: Western Blot, Isolation, Two Tailed Test