S-165 Search Results


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  • 99
    Thermo Fisher 1x dulbecco s165 phosphate buffered saline
    1x Dulbecco S165 Phosphate Buffered Saline, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Rockland Immunochemicals alkaline phosphatase conjugated anti mouse ige
    Alkaline Phosphatase Conjugated Anti Mouse Ige, supplied by Rockland Immunochemicals, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Abnova anti connective tissue growth factor
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    Abcam anti phospho ser165 nfat4
    Cytoplasmic Rephosphorylation of NFAT Isoforms (A) The various steps regulating NFAT dynamics are shown. (B) Treatment with acetate (5 mM) inhibits movement of NFAT1-GFP or <t>NFAT4-GFP</t> to the nucleus in response to thapsigargin stimulation. (C) Aggregate data are compared. Each bar represents mean data from three independent experiments. Acetate was applied 10 min before stimulation and then maintained throughout. (D) NFAT isoform rephosphorylation was measured in cytoplasmic extract at different times after termination of calcineurin activity. (E) Graphs summarize averaged data from two independent experiments, as in (D). (F) Blots compare the kinetics of reposhosphorylation of <t>Ser165</t> on NFAT4 and Ser177 on NFAT1 in cytoplasmic extract. (G) The graph summarizes data from two independent gels, as in (F). Zero intensity at time 0 corresponds to full dephosphorylation of NFAT after thapsigargin stimulation (F). (H) Images compare NFAT1-GFP and NFAT4-GFP nuclear accumulation in experiments using a two-pulse protocol. In the single-pulse protocol, LTC 4 was applied for 2 min in Ca 2+ -containing external solution (pulse 1) and then washed for 38 min in Ca 2+ -free solution (when the image was taken). In the paired-pulse protocol, after the first pulse, cells were washed for 8 min in Ca 2+ -free solution and then a second identical LTC 4 pulse was applied for 2 min in external Ca 2+ . Cells were then exposed to Ca 2+ -free solution for a further 28 min before images were taken. Aggregate data from several independent experiments are shown in the histogram. Each bar is the mean of between 7 and 15 cells. In (C), (E), (G), and (H), data are represented as mean ± SEM. See also Figure S6 .
    Anti Phospho Ser165 Nfat4, supplied by Abcam, used in various techniques. Bioz Stars score: 88/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Image Search Results


    Cytoplasmic Rephosphorylation of NFAT Isoforms (A) The various steps regulating NFAT dynamics are shown. (B) Treatment with acetate (5 mM) inhibits movement of NFAT1-GFP or NFAT4-GFP to the nucleus in response to thapsigargin stimulation. (C) Aggregate data are compared. Each bar represents mean data from three independent experiments. Acetate was applied 10 min before stimulation and then maintained throughout. (D) NFAT isoform rephosphorylation was measured in cytoplasmic extract at different times after termination of calcineurin activity. (E) Graphs summarize averaged data from two independent experiments, as in (D). (F) Blots compare the kinetics of reposhosphorylation of Ser165 on NFAT4 and Ser177 on NFAT1 in cytoplasmic extract. (G) The graph summarizes data from two independent gels, as in (F). Zero intensity at time 0 corresponds to full dephosphorylation of NFAT after thapsigargin stimulation (F). (H) Images compare NFAT1-GFP and NFAT4-GFP nuclear accumulation in experiments using a two-pulse protocol. In the single-pulse protocol, LTC 4 was applied for 2 min in Ca 2+ -containing external solution (pulse 1) and then washed for 38 min in Ca 2+ -free solution (when the image was taken). In the paired-pulse protocol, after the first pulse, cells were washed for 8 min in Ca 2+ -free solution and then a second identical LTC 4 pulse was applied for 2 min in external Ca 2+ . Cells were then exposed to Ca 2+ -free solution for a further 28 min before images were taken. Aggregate data from several independent experiments are shown in the histogram. Each bar is the mean of between 7 and 15 cells. In (C), (E), (G), and (H), data are represented as mean ± SEM. See also Figure S6 .

    Journal: Molecular Cell

    Article Title: Control of NFAT Isoform Activation and NFAT-Dependent Gene Expression through Two Coincident and Spatially Segregated Intracellular Ca2+ Signals

    doi: 10.1016/j.molcel.2016.11.011

    Figure Lengend Snippet: Cytoplasmic Rephosphorylation of NFAT Isoforms (A) The various steps regulating NFAT dynamics are shown. (B) Treatment with acetate (5 mM) inhibits movement of NFAT1-GFP or NFAT4-GFP to the nucleus in response to thapsigargin stimulation. (C) Aggregate data are compared. Each bar represents mean data from three independent experiments. Acetate was applied 10 min before stimulation and then maintained throughout. (D) NFAT isoform rephosphorylation was measured in cytoplasmic extract at different times after termination of calcineurin activity. (E) Graphs summarize averaged data from two independent experiments, as in (D). (F) Blots compare the kinetics of reposhosphorylation of Ser165 on NFAT4 and Ser177 on NFAT1 in cytoplasmic extract. (G) The graph summarizes data from two independent gels, as in (F). Zero intensity at time 0 corresponds to full dephosphorylation of NFAT after thapsigargin stimulation (F). (H) Images compare NFAT1-GFP and NFAT4-GFP nuclear accumulation in experiments using a two-pulse protocol. In the single-pulse protocol, LTC 4 was applied for 2 min in Ca 2+ -containing external solution (pulse 1) and then washed for 38 min in Ca 2+ -free solution (when the image was taken). In the paired-pulse protocol, after the first pulse, cells were washed for 8 min in Ca 2+ -free solution and then a second identical LTC 4 pulse was applied for 2 min in external Ca 2+ . Cells were then exposed to Ca 2+ -free solution for a further 28 min before images were taken. Aggregate data from several independent experiments are shown in the histogram. Each bar is the mean of between 7 and 15 cells. In (C), (E), (G), and (H), data are represented as mean ± SEM. See also Figure S6 .

    Article Snippet: Antibodies against ERK (1:5,000, rabbit polyclonal; Santa Cruz Biotechnology), GFP (1:2,000, rabbit polyclonal; Cell Signaling), HH3 (Histone H3; 1:5,000, rabbit polyclonal; Abcam), InsP3 type I receptor (1:1,000, mouse monoclonal; Abcam), anti-phospho Ser177 NFAT1 (Santa Cruz; 1:2,000 dilution), anti-phospho Ser165 NFAT4 (Abcam; 1:2,000), and IL-5 (Santa Cruz; 1:500) were used.

    Techniques: Activity Assay, De-Phosphorylation Assay