Journal: Journal of Neuroinflammation

Article Title: Estrogens regulate neuroinflammatory genes via estrogen receptors α and β in the frontal cortex of middle-aged female rats

doi: 10.1186/1742-2094-8-82

Figure Lengend Snippet: Microarray analysis revealed 87 ERα agonist-regulated genes which were categorized based on function . ERα agonist-regulated genes included twenty-one immunity/inflammation genes. The cluster contained S100 calcium-binding (S100a9, S100a8) and defense proteins (RatNP-3b, Np4, Defa, Camp), complement C3 and Serping1, Ig chains (Igha, IgG-2a, Igj), mast cell proteases (Mcpt8, Mcpt9) and MHC antigens (RT1-Aw2, RT1-N1).

Article Snippet: RT1-Aw2 , RT1 class Ib , 1388203_x_at , 0.536 , Rn03034964_u1 , 0.658.

Techniques: Microarray, Binding Assay

Journal: Journal of Neuroinflammation

Article Title: Estrogens regulate neuroinflammatory genes via estrogen receptors α and β in the frontal cortex of middle-aged female rats

doi: 10.1186/1742-2094-8-82

Figure Lengend Snippet: Confirmation of the ERα agonist-regulated immunity/inflammation genes by quantitative real-time PCR.

Article Snippet: RT1-Aw2 , RT1 class Ib , 1388203_x_at , 0.536 , Rn03034964_u1 , 0.658.

Techniques: Microarray, Real-time Polymerase Chain Reaction, Binding Assay

Journal: Journal of Neuroinflammation

Article Title: Estrogens regulate neuroinflammatory genes via estrogen receptors α and β in the frontal cortex of middle-aged female rats

doi: 10.1186/1742-2094-8-82

Figure Lengend Snippet: Real-time PCR revealed that E2 regulated the transcription of neuroinflammatory genes in the frontal cortex of middle-aged female rats.

Article Snippet: RT1-Aw2 , RT1 class Ib , 1388203_x_at , 0.536 , Rn03034964_u1 , 0.658.

Techniques: Real-time Polymerase Chain Reaction

Journal: Journal of Neuroinflammation

Article Title: Estrogens regulate neuroinflammatory genes via estrogen receptors α and β in the frontal cortex of middle-aged female rats

doi: 10.1186/1742-2094-8-82

Figure Lengend Snippet: The effects of ERα agonist 16α-LE2 and ERβ agonist DPN on the transcription of E2-regulated neuroinflammatory genes.

Article Snippet: RT1-Aw2 , RT1 class Ib , 1388203_x_at , 0.536 , Rn03034964_u1 , 0.658.

Techniques:

Journal: Journal of Neuroinflammation

Article Title: Estrogens regulate neuroinflammatory genes via estrogen receptors α and β in the frontal cortex of middle-aged female rats

doi: 10.1186/1742-2094-8-82

Figure Lengend Snippet: Summary of estrogenic regulation of neuroinflammatory genes.

Article Snippet: RT1-Aw2 , RT1 class Ib , 1388203_x_at , 0.536 , Rn03034964_u1 , 0.658.

Techniques:

Journal: Cancer Microenvironment

Article Title: Participation of Tumor-Associated Myeloid Cells in Progression of Amelanotic Melanoma (RMM Tumor Line) in F344 Rats, with Particular Reference to MHC Class II- and CD163-Expressing Cells

doi: 10.1007/s12307-017-0193-x

Figure Lengend Snippet: Probes used in the real-time RT-PCR

Article Snippet: The data were analyzed using the comparative C t method ( ΔΔ C t method). table ft1 table-wrap mode="anchored" t5 Table 1 caption a7 Symbol Name Assay ID RT1-Ba RT1 class II, locus Ba Rn01428452_m1 IFN-γ Interferon gamma Rn00594078_m1 M-CSF Colony stimulating factor-1 Rn00572010_m1 GM-CSF Colony stimulating factor-2 Rn01456850_m1 TNF-α Tumor necrosis factor-alpha Rn01525859_g1 TGF-β1 Transforming growth factor-beta 1 Rn00572010_m1 Il-1β Interleukin 1 beta Rn00580432_m1 IL-6 Interleukin-6 Rn01410330_m1 IL-10 Interleukin-10 Rn00563409_m1 IL-12a Interleukin-12a Rn00584538_m1 CCL2 Chemokine (C-C motif) ligand 2 Rn00580555_m1 CCL5 Chemokine (C-C motif) ligand 5 Rn00579590_m1 CXCL1 C-X-C motif chemokine ligand 1 Rn00578225_m1 ΗΙΦ−1α Hypoxia inducible factor-1 alpha Rn01472831_m1 VEGFa Vascular endothelial growth factor A Rn01511602_m1 Flt-1 FMS-related tyrosine kinase 1 Rn01409533_m1 18 s rRNA Eukaryotic 18S rRNA Hs99999901_s1 Open in a separate window Probes used in the real-time RT-PCR table ft1 table-wrap mode="anchored" t5 Table 2 caption a7 Gene name Sequence Galectin-3 (forward) 5′-ATCCTGCTACTGGCCCTTT-3′ (reverse) 5′-GCGATGTCGTTCCCTTTCTT-3′ MMP-2 (forward) 5’GGGTCTATTCTGCCAGCACTTT-3′ (reverse) 5’CGGGGTCCATTTTCTTCTTTACTT-3′ MMP-9 (forward) 5’TGCCCTGGAACTCACACAAC-3′ (reverse) 5’GGAAACTCACACGCCAGAAG-3’ TIMP-1 (forward) 5’CCTCTGGCATCCTCTTGTTG-3′ (reverse) 5’GGTGGTCTCGATGATTTCTGG-3’ TIMP-2 (forward) 5’CGTTTTGCAATGCAGACGTA-3′ (reverse) 5’GGCCGTGTAGATAAATTCGATG-3’ PDGFb (forward) 5’TCTGGCCTGCAAGTGTGAG-3′ (reverse) 5’CCCGAGTTTGAGGTGTCTTG-3’ 18 s rRNA (forward) 5’-GTAACCCGTTGAACCCCATT-3′ (reverse) 5′-CCATCCAATCGGTAGTAGCG-3’ Open in a separate window Primers used in the real-time RT-PCR

Techniques:

Journal: Cancer Microenvironment

Article Title: Participation of Tumor-Associated Myeloid Cells in Progression of Amelanotic Melanoma (RMM Tumor Line) in F344 Rats, with Particular Reference to MHC Class II- and CD163-Expressing Cells

doi: 10.1007/s12307-017-0193-x

Figure Lengend Snippet: Laser microdissection (LMD) and real-time RT-PCR for MHC II-HIGH and MHC II-LOW regions in RMM nodules (0.5 cm and 3 cm). (a) example of MHC II-HIGH and MHC-LOW areas detected by immunofluorescence staining with Alexa 488-conjugated anti-MHC class II, before microdissection. Real-time RT-PCR for RT1-Ba (b), IFN-γ (c), GM-CSF (d), IL-12a (e), M-CSF (f), TGF-β1 (g), IL-10 (h) and HIF-1α (i). Tukey’s test *P < 0.05, **P < 0.001, significantly different from MHC II-LOW within each size point; †P < 0.05, ††P < 0.001, significantly different from MHC II-HIGH in 0.5 cm tumors. Bar, 50 μm

Article Snippet: The data were analyzed using the comparative C t method ( ΔΔ C t method). table ft1 table-wrap mode="anchored" t5 Table 1 caption a7 Symbol Name Assay ID RT1-Ba RT1 class II, locus Ba Rn01428452_m1 IFN-γ Interferon gamma Rn00594078_m1 M-CSF Colony stimulating factor-1 Rn00572010_m1 GM-CSF Colony stimulating factor-2 Rn01456850_m1 TNF-α Tumor necrosis factor-alpha Rn01525859_g1 TGF-β1 Transforming growth factor-beta 1 Rn00572010_m1 Il-1β Interleukin 1 beta Rn00580432_m1 IL-6 Interleukin-6 Rn01410330_m1 IL-10 Interleukin-10 Rn00563409_m1 IL-12a Interleukin-12a Rn00584538_m1 CCL2 Chemokine (C-C motif) ligand 2 Rn00580555_m1 CCL5 Chemokine (C-C motif) ligand 5 Rn00579590_m1 CXCL1 C-X-C motif chemokine ligand 1 Rn00578225_m1 ΗΙΦ−1α Hypoxia inducible factor-1 alpha Rn01472831_m1 VEGFa Vascular endothelial growth factor A Rn01511602_m1 Flt-1 FMS-related tyrosine kinase 1 Rn01409533_m1 18 s rRNA Eukaryotic 18S rRNA Hs99999901_s1 Open in a separate window Probes used in the real-time RT-PCR table ft1 table-wrap mode="anchored" t5 Table 2 caption a7 Gene name Sequence Galectin-3 (forward) 5′-ATCCTGCTACTGGCCCTTT-3′ (reverse) 5′-GCGATGTCGTTCCCTTTCTT-3′ MMP-2 (forward) 5’GGGTCTATTCTGCCAGCACTTT-3′ (reverse) 5’CGGGGTCCATTTTCTTCTTTACTT-3′ MMP-9 (forward) 5’TGCCCTGGAACTCACACAAC-3′ (reverse) 5’GGAAACTCACACGCCAGAAG-3’ TIMP-1 (forward) 5’CCTCTGGCATCCTCTTGTTG-3′ (reverse) 5’GGTGGTCTCGATGATTTCTGG-3’ TIMP-2 (forward) 5’CGTTTTGCAATGCAGACGTA-3′ (reverse) 5’GGCCGTGTAGATAAATTCGATG-3’ PDGFb (forward) 5’TCTGGCCTGCAAGTGTGAG-3′ (reverse) 5’CCCGAGTTTGAGGTGTCTTG-3’ 18 s rRNA (forward) 5’-GTAACCCGTTGAACCCCATT-3′ (reverse) 5′-CCATCCAATCGGTAGTAGCG-3’ Open in a separate window Primers used in the real-time RT-PCR

Techniques: Laser Capture Microdissection, Quantitative RT-PCR, Immunofluorescence, Staining

Journal: Journal of Neuroinflammation

Article Title: Estrogens regulate neuroinflammatory genes via estrogen receptors α and β in the frontal cortex of middle-aged female rats

doi: 10.1186/1742-2094-8-82

Figure Lengend Snippet: Microarray analysis revealed 87 ERα agonist-regulated genes which were categorized based on function . ERα agonist-regulated genes included twenty-one immunity/inflammation genes. The cluster contained S100 calcium-binding (S100a9, S100a8) and defense proteins (RatNP-3b, Np4, Defa, Camp), complement C3 and Serping1, Ig chains (Igha, IgG-2a, Igj), mast cell proteases (Mcpt8, Mcpt9) and MHC antigens (RT1-Aw2, RT1-N1).

Article Snippet: RT1-N1 , RT1 class Ib, locus N1 , 1387839_at , 0.570 , Rn00561858_m1 , 0.654.

Techniques: Microarray, Binding Assay

Journal: Journal of Neuroinflammation

Article Title: Estrogens regulate neuroinflammatory genes via estrogen receptors α and β in the frontal cortex of middle-aged female rats

doi: 10.1186/1742-2094-8-82

Figure Lengend Snippet: Confirmation of the ERα agonist-regulated immunity/inflammation genes by quantitative real-time PCR.

Article Snippet: RT1-N1 , RT1 class Ib, locus N1 , 1387839_at , 0.570 , Rn00561858_m1 , 0.654.

Techniques: Microarray, Real-time Polymerase Chain Reaction, Binding Assay

Journal: Journal of Neuroinflammation

Article Title: Estrogens regulate neuroinflammatory genes via estrogen receptors α and β in the frontal cortex of middle-aged female rats

doi: 10.1186/1742-2094-8-82

Figure Lengend Snippet: Real-time PCR revealed that E2 regulated the transcription of neuroinflammatory genes in the frontal cortex of middle-aged female rats.

Article Snippet: RT1-N1 , RT1 class Ib, locus N1 , 1387839_at , 0.570 , Rn00561858_m1 , 0.654.

Techniques: Real-time Polymerase Chain Reaction

Journal: Journal of Neuroinflammation

Article Title: Estrogens regulate neuroinflammatory genes via estrogen receptors α and β in the frontal cortex of middle-aged female rats

doi: 10.1186/1742-2094-8-82

Figure Lengend Snippet: The effects of ERα agonist 16α-LE2 and ERβ agonist DPN on the transcription of E2-regulated neuroinflammatory genes.

Article Snippet: RT1-N1 , RT1 class Ib, locus N1 , 1387839_at , 0.570 , Rn00561858_m1 , 0.654.

Techniques:

Journal: Journal of Neuroinflammation

Article Title: Estrogens regulate neuroinflammatory genes via estrogen receptors α and β in the frontal cortex of middle-aged female rats

doi: 10.1186/1742-2094-8-82

Figure Lengend Snippet: Summary of estrogenic regulation of neuroinflammatory genes.

Article Snippet: RT1-N1 , RT1 class Ib, locus N1 , 1387839_at , 0.570 , Rn00561858_m1 , 0.654.

Techniques: