Journal: BMC Veterinary Research
Article Title: Stimulation of bovine monocyte-derived macrophages with lipopolysaccharide, interferon-ɣ, Interleukin-4 or Interleukin-13 does not induce detectable changes in nitric oxide or arginase activity
Figure Lengend Snippet: Levels of nitric oxide (1a 1b) and chitinase (1e 1f) in monocyte-derived and splenic macrophage cell supernatants and arginase (1c 1d) in cell lysates following stimulation with LPS and/or cytokines (LPS 1000 ng/ml, IFNɣ 20 ng/ml, IL-4 20 ng/ml and IL-13 20 ng/ml) for 40 h. Levels of enzyme product were determined by measuring OD values and comparison with standard curves. Means and standard errors of data from triplicate cultures from representative individual animals are shown, monocyte-derived macrophages all being obtained from one animal and splenic macrophages all from another individual. Similar findings were recorded from all individuals tested ( n = 5 MDM, n = 7 SM) for stimulation times of 12–72 h
Article Snippet: MDM or SM were stimulated by addition of 50–2000 ng/ml LPS from E. coli 0111:B4 (Insight Biotechnology Ltd., Middlesex) and/or 20-100 ng/ml bovine IFNγ (ThermoFisher Scientific, UK) or with 10-40 ng/ml recombinant bovine IL-4 (Kingfisher Biotech Inc., St Paul) and/or 10-20 ng/ml recombinant bovine IL-13 (Kingfisher Biotech Inc).
Techniques: Derivative Assay