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    Kingfisher Biotech recombinant bovine il 4
    IL-6 concentration in supernatants from splenic macrophages stimulated with LPS (1000 ng/ml) and IFNγ (20 ng/ml) or <t>IL-4</t> (20 ng/ml) for 20 h. OD values were determined from samples comprising combined supernatants from triplicate wells, run in duplicate. Results for two individual animals ( a and b ) are shown
    Recombinant Bovine Il 4, supplied by Kingfisher Biotech, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/recombinant bovine il 4/product/Kingfisher Biotech
    Average 94 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    recombinant bovine il 4 - by Bioz Stars, 2022-07
    94/100 stars
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    IL-6 concentration in supernatants from splenic macrophages stimulated with LPS (1000 ng/ml) and IFNγ (20 ng/ml) or IL-4 (20 ng/ml) for 20 h. OD values were determined from samples comprising combined supernatants from triplicate wells, run in duplicate. Results for two individual animals ( a and b ) are shown

    Journal: BMC Veterinary Research

    Article Title: Stimulation of bovine monocyte-derived macrophages with lipopolysaccharide, interferon-ɣ, Interleukin-4 or Interleukin-13 does not induce detectable changes in nitric oxide or arginase activity

    doi: 10.1186/s12917-019-1785-0

    Figure Lengend Snippet: IL-6 concentration in supernatants from splenic macrophages stimulated with LPS (1000 ng/ml) and IFNγ (20 ng/ml) or IL-4 (20 ng/ml) for 20 h. OD values were determined from samples comprising combined supernatants from triplicate wells, run in duplicate. Results for two individual animals ( a and b ) are shown

    Article Snippet: MDM or SM were stimulated by addition of 50–2000 ng/ml LPS from E. coli 0111:B4 (Insight Biotechnology Ltd., Middlesex) and/or 20-100 ng/ml bovine IFNγ (ThermoFisher Scientific, UK) or with 10-40 ng/ml recombinant bovine IL-4 (Kingfisher Biotech Inc., St Paul) and/or 10-20 ng/ml recombinant bovine IL-13 (Kingfisher Biotech Inc).

    Techniques: Concentration Assay

    Fold changes in MDM production of nos2 ( a ) and arg1 ( b ) following stimulation with LPS (1000 ng/ml) IFNγ (20 ng/ml) or IL-4 (20 ng/ml) IL-13 (20 ng/ml) compared with changes in control cells, normalised with reference to transcription levels of GAPDH at 12 24 h. Fold changes in levels of nitric oxide in MDM cell supernatants from stimulated cells in comparison to unstimulated controls are shown in c . Supernatants and mRNA were harvested contemporaneously from the same cell populations and were performed in triplicate. Data from the same representative individual are shown in a , b and c

    Journal: BMC Veterinary Research

    Article Title: Stimulation of bovine monocyte-derived macrophages with lipopolysaccharide, interferon-ɣ, Interleukin-4 or Interleukin-13 does not induce detectable changes in nitric oxide or arginase activity

    doi: 10.1186/s12917-019-1785-0

    Figure Lengend Snippet: Fold changes in MDM production of nos2 ( a ) and arg1 ( b ) following stimulation with LPS (1000 ng/ml) IFNγ (20 ng/ml) or IL-4 (20 ng/ml) IL-13 (20 ng/ml) compared with changes in control cells, normalised with reference to transcription levels of GAPDH at 12 24 h. Fold changes in levels of nitric oxide in MDM cell supernatants from stimulated cells in comparison to unstimulated controls are shown in c . Supernatants and mRNA were harvested contemporaneously from the same cell populations and were performed in triplicate. Data from the same representative individual are shown in a , b and c

    Article Snippet: MDM or SM were stimulated by addition of 50–2000 ng/ml LPS from E. coli 0111:B4 (Insight Biotechnology Ltd., Middlesex) and/or 20-100 ng/ml bovine IFNγ (ThermoFisher Scientific, UK) or with 10-40 ng/ml recombinant bovine IL-4 (Kingfisher Biotech Inc., St Paul) and/or 10-20 ng/ml recombinant bovine IL-13 (Kingfisher Biotech Inc).

    Techniques:

    Levels of nitric oxide (1a 1b) and chitinase (1e 1f) in monocyte-derived and splenic macrophage cell supernatants and arginase (1c 1d) in cell lysates following stimulation with LPS and/or cytokines (LPS 1000 ng/ml, IFNɣ 20 ng/ml, IL-4 20 ng/ml and IL-13 20 ng/ml) for 40 h. Levels of enzyme product were determined by measuring OD values and comparison with standard curves. Means and standard errors of data from triplicate cultures from representative individual animals are shown, monocyte-derived macrophages all being obtained from one animal and splenic macrophages all from another individual. Similar findings were recorded from all individuals tested ( n = 5 MDM, n = 7 SM) for stimulation times of 12–72 h

    Journal: BMC Veterinary Research

    Article Title: Stimulation of bovine monocyte-derived macrophages with lipopolysaccharide, interferon-ɣ, Interleukin-4 or Interleukin-13 does not induce detectable changes in nitric oxide or arginase activity

    doi: 10.1186/s12917-019-1785-0

    Figure Lengend Snippet: Levels of nitric oxide (1a 1b) and chitinase (1e 1f) in monocyte-derived and splenic macrophage cell supernatants and arginase (1c 1d) in cell lysates following stimulation with LPS and/or cytokines (LPS 1000 ng/ml, IFNɣ 20 ng/ml, IL-4 20 ng/ml and IL-13 20 ng/ml) for 40 h. Levels of enzyme product were determined by measuring OD values and comparison with standard curves. Means and standard errors of data from triplicate cultures from representative individual animals are shown, monocyte-derived macrophages all being obtained from one animal and splenic macrophages all from another individual. Similar findings were recorded from all individuals tested ( n = 5 MDM, n = 7 SM) for stimulation times of 12–72 h

    Article Snippet: MDM or SM were stimulated by addition of 50–2000 ng/ml LPS from E. coli 0111:B4 (Insight Biotechnology Ltd., Middlesex) and/or 20-100 ng/ml bovine IFNγ (ThermoFisher Scientific, UK) or with 10-40 ng/ml recombinant bovine IL-4 (Kingfisher Biotech Inc., St Paul) and/or 10-20 ng/ml recombinant bovine IL-13 (Kingfisher Biotech Inc).

    Techniques: Derivative Assay