Journal: Frontiers in Physiology

Article Title: miR-146a is a critical target associated with multiple biological pathways of skin aging

doi: 10.3389/fphys.2024.1291344

Figure Lengend Snippet: Expression of miR-146a in NHDFs (normal human dermal fibroblasts) decreases with age. (A) Detection of miR-146a (white) with SmartFlare™ RNA Detection Probe in 19y, 27y, 40y, and 62y old NHDFs. (B) miR-146a expression in 62y old NHDF relative to 19y old NHDF assayed by qRT-PCR. ** p = 0.0001; Error bars ± SEM; qRT-PCR, quantitative Real-Time Polymerase Chain Reaction. Data are representative of at least two independent experiments.

Article Snippet: The next day, cells were incubated with SmartFlareTM RNA Detection Probe miR-146a (Merck Millipore, SmartFlareTM probe miR-146a, Cat# SF-500).

Techniques: Expressing, RNA Detection, Quantitative RT-PCR, Real-time Polymerase Chain Reaction

Journal: Frontiers in Physiology

Article Title: miR-146a is a critical target associated with multiple biological pathways of skin aging

doi: 10.3389/fphys.2024.1291344

Figure Lengend Snippet: miR-146a inhibition impacts the clock gene machinery in NHDFs. miR-146a inhibition in 19y old NHDF leads to a decrease of Bmal-1 and in 62y old NHDF to a decrease of Bmal-1, Per-1 and Clock assayed by qRT-PCR. ** p = 0.001, *** p = 0.0001. Dh#3, Dharmafect #3; Error bars ± SEM; Data are representative of at least two independent experiments.

Article Snippet: The next day, cells were incubated with SmartFlareTM RNA Detection Probe miR-146a (Merck Millipore, SmartFlareTM probe miR-146a, Cat# SF-500).

Techniques: Inhibition, Quantitative RT-PCR

Journal: Frontiers in Physiology

Article Title: miR-146a is a critical target associated with multiple biological pathways of skin aging

doi: 10.3389/fphys.2024.1291344

Figure Lengend Snippet: (A) miR-146a inhibition impacts cellular proliferation in NHDFs. Cell count of 19y old and 62y old NHDFs subjected to 5 days of miR-146a inhibition. Cell count was performed each day post treatment on both Dh#3 (Vehicle Ctrl) and miR-146a inhibitor-treated samples. (B) miR-146a inhibition impacts cytokines production in NHDFs. Cytokines analysis of the spent media from both 19y old and 62y old NHDFs subjected to 5 days of miR-146a inhibition. Error bars ± SEM are plotted for both cell lines. ** p = 0.01, *** p < 0.001, **** p < 0.0001. Dh#3, Dharmafect #3; Data are representative of at least two independent experiments.

Article Snippet: The next day, cells were incubated with SmartFlareTM RNA Detection Probe miR-146a (Merck Millipore, SmartFlareTM probe miR-146a, Cat# SF-500).

Techniques: Inhibition, Cell Counting

Journal: Frontiers in Physiology

Article Title: miR-146a is a critical target associated with multiple biological pathways of skin aging

doi: 10.3389/fphys.2024.1291344

Figure Lengend Snippet: miR-146a inhibition impacts the release of pro-collagen type I and MMPs in NHDFs. (A) miR-146a inhibition leads to a decrease of pro-collagen type I in 19y old NHDF assayed by pro-collagen type I ELISA kit. (B) miR-146a inhibition in 62y old NHDF leads to a significant increase of MMP-1, MMP-9 and MMP-10 assayed by Multiplex ELISA measurement. **** p < 0.0001. Error bars ± SEM; Dh#3, Dharmafect #3; MMP, Matrix Metalloproteinase; Data are representative of at least two independent experiments.

Article Snippet: The next day, cells were incubated with SmartFlareTM RNA Detection Probe miR-146a (Merck Millipore, SmartFlareTM probe miR-146a, Cat# SF-500).

Techniques: Inhibition, Enzyme-linked Immunosorbent Assay, Multiplex Assay

Journal: Frontiers in Physiology

Article Title: miR-146a is a critical target associated with multiple biological pathways of skin aging

doi: 10.3389/fphys.2024.1291344

Figure Lengend Snippet: miR-146a inhibition increases DNA damage in NHDFs. miR-146a inhibition in 19y old and 62y old NHDFs leads to increased DNA damage (depicted in the graph as tail moment) measured by the comet assay and shown by the associated pictures beneath. **** p < 0.0001. Error bars ± SEM. Data are representative of at least two independent experiments.

Article Snippet: The next day, cells were incubated with SmartFlareTM RNA Detection Probe miR-146a (Merck Millipore, SmartFlareTM probe miR-146a, Cat# SF-500).

Techniques: Inhibition, Single Cell Gel Electrophoresis

Journal: Frontiers in Physiology

Article Title: miR-146a is a critical target associated with multiple biological pathways of skin aging

doi: 10.3389/fphys.2024.1291344

Figure Lengend Snippet: Adansonia digitata treatment increases the expression of miR-146a in NHDFs. (A) miR-146a expression was assayed by ViewRNA ISH assay before and after 2% Adansonia digitata treatment as shown by the associated confocal images. (B) miR-146a gene expression levels before and after treatments with Adansonia digitata were measured by qRT-PCR in 19y old and 62y old NHDFs. *** p = 0.001, **** p < 0.0001. ISH, In situ hybridization; Error bars ± SEM. Data are representative of at least three independent experiments.

Article Snippet: The next day, cells were incubated with SmartFlareTM RNA Detection Probe miR-146a (Merck Millipore, SmartFlareTM probe miR-146a, Cat# SF-500).

Techniques: Expressing, In Situ Hybridization, Gene Expression, Quantitative RT-PCR

Journal: Frontiers in Physiology

Article Title: miR-146a is a critical target associated with multiple biological pathways of skin aging

doi: 10.3389/fphys.2024.1291344

Figure Lengend Snippet: miR-146a is transported through exosomes in NHDFs. Naïve 62y old NHDF were incubated with the media of 19y old NHDF previously treated with 2% Adansonia digitata. Gene expression levels of non-exosomal (cellular fraction) and exosomal (supernatant fraction) miR-146a were assayed by qRT-PCR. * p < 0.05, **** p < 0.0001. Error bars ± SEM. Data are representative of at least three independent experiments.

Article Snippet: The next day, cells were incubated with SmartFlareTM RNA Detection Probe miR-146a (Merck Millipore, SmartFlareTM probe miR-146a, Cat# SF-500).

Techniques: Incubation, Gene Expression, Quantitative RT-PCR

Journal: PLoS Neglected Tropical Diseases

Article Title: Susceptibility of Armigeres subalbatus Coquillett (Diptera: Culicidae) to Zika virus through oral and urine infection

doi: 10.1371/journal.pntd.0008450

Figure Lengend Snippet: ZIKV infection rate and quantification of Zika viral RNA copies, by real-time RT-PCR, detected in the salivary gland, midgut, ovary and collected saliva of Ar . subalbatus females at 4, 7, and 10 dpe (blood meal).

Article Snippet: ZIKV in these organs was detected using a One-Step Detection Kit for Zika Virus RNA (Daan Gene, Guangzhou, China), and PCR was conducted as previously described Li et al., 2017 [ ].

Techniques: Infection, Quantitative RT-PCR