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    Additional name(s) for this target protein: BTB/POZ domain-containing protein 7
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    86
    Abiocode Inc r3399 1
    R3399 1, supplied by Abiocode Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/r3399 1/product/Abiocode Inc
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    r3399 1 - by Bioz Stars, 2024-02
    86/100 stars
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    86
    Millipore polyclonal anti vps8
    ( A ) Schematic representation of VPS41 and <t>VPS8.</t> ( B ) Schematic representation of the RING domain homology between VPS41 and VPS8. ( C ) GST pulldown (PD) experiments show that purified VPS18 RING domain is efficiently captured by the VPS41 RING domain, but not by the VPS8 RING domain (Coomassie). ( D ) Full-length VPS41 produced by in vitro transcription/translation is pulled down by the VPS18 RING domain, but full-length VPS8 is not. ( E ) In HEK293T cells, the VPS8 RING long domain co-immunoprecipitates endogenous VPS18 — albeit less efficiently than the VPS41 RING domain — but the VPS8 RING short construct does not.
    Polyclonal Anti Vps8, supplied by Millipore, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/polyclonal anti vps8/product/Millipore
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    polyclonal anti vps8 - by Bioz Stars, 2024-02
    86/100 stars
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    86
    Abiocode Inc anti fca
    ( A ) Schematic representation of VPS41 and <t>VPS8.</t> ( B ) Schematic representation of the RING domain homology between VPS41 and VPS8. ( C ) GST pulldown (PD) experiments show that purified VPS18 RING domain is efficiently captured by the VPS41 RING domain, but not by the VPS8 RING domain (Coomassie). ( D ) Full-length VPS41 produced by in vitro transcription/translation is pulled down by the VPS18 RING domain, but full-length VPS8 is not. ( E ) In HEK293T cells, the VPS8 RING long domain co-immunoprecipitates endogenous VPS18 — albeit less efficiently than the VPS41 RING domain — but the VPS8 RING short construct does not.
    Anti Fca, supplied by Abiocode Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/anti fca/product/Abiocode Inc
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    anti fca - by Bioz Stars, 2024-02
    86/100 stars
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    86
    Abiocode Inc immunoblotting with anti fca fca n antibody
    a , b The degradation of SSF414D was slower than that of SSF414N in the cell-free protein degradation assay. Recombinant purified His-SSF414N/414D was incubated in equal amounts of total proteins extracted from 14-day-old wild-type Col-0 seedlings in the presence of ATP. His-SSF414N/414D was detected with an anti-His antibody. Representative images are shown in ( a ), and relative protein levels are shown in ( b ). In ( b ), data are presented as mean ± SEM, and n = 10 independent replicates. c , d Stability of SSF414N/414D-GFP in vivo. Fourteen-day-old SSF414N-GFP and SSF414D-GFP transgenic seedlings were treated with 50 μM MG132. In ( c , d ), SSF-GFP- and DAPI-stained nuclear signals in roots of 18 independent lines were collected by confocal microscopy and quantitatively analyzed under the same set of fluorescence intensities and at the same scale. From each line, more than 10 seedlings were assayed. Representative pictures are shown in ( c ), and the relative GFP signal level is normalized to DAPI in ( d ). In ( d ), box plot: lower vertical bar = sample minimum, lower box = lower quartile, middle line = median, upper box = upper quartile, upper vertical bar = sample maximum, single points = outliers. e , f Detection of SSF-GFP in vivo from Col-0, SSF414N-GFP, and SSF414D-GFP plants. Total protein was extracted from whole seedlings of 14-day-old Col-0, SSF414N-GFP, and SSF414D-GFP plants, incubated with GFP-trap to enrich GFP-fused protein, and analyzed by <t>immunoblotting</t> using an anti-GFP antibody. Total protein was used as the input loading control. In ( f ), data are presented as mean ± SEM, and n = 10 independent replicates. These experiments were repeated at least three times, and consistent results were obtained. In ( b , d , f ), asterisks indicate significant differences (* p < 0.05; ** p < 0.01; *** p < 0.001, ns, not significant; two-tailed unpaired t test). Source data are provided as a Source data file.
    Immunoblotting With Anti Fca Fca N Antibody, supplied by Abiocode Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/immunoblotting with anti fca fca n antibody/product/Abiocode Inc
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    immunoblotting with anti fca fca n antibody - by Bioz Stars, 2024-02
    86/100 stars
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    Image Search Results


    ( A ) Schematic representation of VPS41 and VPS8. ( B ) Schematic representation of the RING domain homology between VPS41 and VPS8. ( C ) GST pulldown (PD) experiments show that purified VPS18 RING domain is efficiently captured by the VPS41 RING domain, but not by the VPS8 RING domain (Coomassie). ( D ) Full-length VPS41 produced by in vitro transcription/translation is pulled down by the VPS18 RING domain, but full-length VPS8 is not. ( E ) In HEK293T cells, the VPS8 RING long domain co-immunoprecipitates endogenous VPS18 — albeit less efficiently than the VPS41 RING domain — but the VPS8 RING short construct does not.

    Journal: Biochemical Journal

    Article Title: VPS18 recruits VPS41 to the human HOPS complex via a RING–RING interaction

    doi: 10.1042/BCJ20170588

    Figure Lengend Snippet: ( A ) Schematic representation of VPS41 and VPS8. ( B ) Schematic representation of the RING domain homology between VPS41 and VPS8. ( C ) GST pulldown (PD) experiments show that purified VPS18 RING domain is efficiently captured by the VPS41 RING domain, but not by the VPS8 RING domain (Coomassie). ( D ) Full-length VPS41 produced by in vitro transcription/translation is pulled down by the VPS18 RING domain, but full-length VPS8 is not. ( E ) In HEK293T cells, the VPS8 RING long domain co-immunoprecipitates endogenous VPS18 — albeit less efficiently than the VPS41 RING domain — but the VPS8 RING short construct does not.

    Article Snippet: Three antibodies against VPS8 were tested, but failed our validation assays for immunoblotting: polyclonal anti-VPS8 (Proteintech, cat. 15079-1-AP), polyclonal anti-VPS8 (Sigma, cat. HPA036871, lot R33997), and monoclonal anti-VPS8 (Sigma, cat. WH0023355M1-100UG).

    Techniques: Purification, Produced, In Vitro, Construct

    a , b The degradation of SSF414D was slower than that of SSF414N in the cell-free protein degradation assay. Recombinant purified His-SSF414N/414D was incubated in equal amounts of total proteins extracted from 14-day-old wild-type Col-0 seedlings in the presence of ATP. His-SSF414N/414D was detected with an anti-His antibody. Representative images are shown in ( a ), and relative protein levels are shown in ( b ). In ( b ), data are presented as mean ± SEM, and n = 10 independent replicates. c , d Stability of SSF414N/414D-GFP in vivo. Fourteen-day-old SSF414N-GFP and SSF414D-GFP transgenic seedlings were treated with 50 μM MG132. In ( c , d ), SSF-GFP- and DAPI-stained nuclear signals in roots of 18 independent lines were collected by confocal microscopy and quantitatively analyzed under the same set of fluorescence intensities and at the same scale. From each line, more than 10 seedlings were assayed. Representative pictures are shown in ( c ), and the relative GFP signal level is normalized to DAPI in ( d ). In ( d ), box plot: lower vertical bar = sample minimum, lower box = lower quartile, middle line = median, upper box = upper quartile, upper vertical bar = sample maximum, single points = outliers. e , f Detection of SSF-GFP in vivo from Col-0, SSF414N-GFP, and SSF414D-GFP plants. Total protein was extracted from whole seedlings of 14-day-old Col-0, SSF414N-GFP, and SSF414D-GFP plants, incubated with GFP-trap to enrich GFP-fused protein, and analyzed by immunoblotting using an anti-GFP antibody. Total protein was used as the input loading control. In ( f ), data are presented as mean ± SEM, and n = 10 independent replicates. These experiments were repeated at least three times, and consistent results were obtained. In ( b , d , f ), asterisks indicate significant differences (* p < 0.05; ** p < 0.01; *** p < 0.001, ns, not significant; two-tailed unpaired t test). Source data are provided as a Source data file.

    Journal: Nature Communications

    Article Title: Molecular variation in a functionally divergent homolog of FCA regulates flowering time in Arabidopsis thaliana

    doi: 10.1038/s41467-020-19666-0

    Figure Lengend Snippet: a , b The degradation of SSF414D was slower than that of SSF414N in the cell-free protein degradation assay. Recombinant purified His-SSF414N/414D was incubated in equal amounts of total proteins extracted from 14-day-old wild-type Col-0 seedlings in the presence of ATP. His-SSF414N/414D was detected with an anti-His antibody. Representative images are shown in ( a ), and relative protein levels are shown in ( b ). In ( b ), data are presented as mean ± SEM, and n = 10 independent replicates. c , d Stability of SSF414N/414D-GFP in vivo. Fourteen-day-old SSF414N-GFP and SSF414D-GFP transgenic seedlings were treated with 50 μM MG132. In ( c , d ), SSF-GFP- and DAPI-stained nuclear signals in roots of 18 independent lines were collected by confocal microscopy and quantitatively analyzed under the same set of fluorescence intensities and at the same scale. From each line, more than 10 seedlings were assayed. Representative pictures are shown in ( c ), and the relative GFP signal level is normalized to DAPI in ( d ). In ( d ), box plot: lower vertical bar = sample minimum, lower box = lower quartile, middle line = median, upper box = upper quartile, upper vertical bar = sample maximum, single points = outliers. e , f Detection of SSF-GFP in vivo from Col-0, SSF414N-GFP, and SSF414D-GFP plants. Total protein was extracted from whole seedlings of 14-day-old Col-0, SSF414N-GFP, and SSF414D-GFP plants, incubated with GFP-trap to enrich GFP-fused protein, and analyzed by immunoblotting using an anti-GFP antibody. Total protein was used as the input loading control. In ( f ), data are presented as mean ± SEM, and n = 10 independent replicates. These experiments were repeated at least three times, and consistent results were obtained. In ( b , d , f ), asterisks indicate significant differences (* p < 0.05; ** p < 0.01; *** p < 0.001, ns, not significant; two-tailed unpaired t test). Source data are provided as a Source data file.

    Article Snippet: SDS-PAGE was used to separate the proteins, and the target proteins were detected by immunoblotting with Anti-FCA (FCA(N) antibody) (Abiocode, R3399-1, dilution: 1:2000), or Anti-Ubiquitin antibody [Ubi-1] (Abcam, ab7254, dilution: 1:2000) antibodies.

    Techniques: Degradation Assay, Recombinant, Purification, Incubation, In Vivo, Transgenic Assay, Staining, Confocal Microscopy, Fluorescence, Western Blot, Two Tailed Test