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  • 86
    Hamamatsu r3292 pspmt
    R3292 Pspmt, supplied by Hamamatsu, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/r3292 pspmt/product/Hamamatsu
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    r3292 pspmt - by Bioz Stars, 2024-02
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    86
    Hamamatsu r3292 02 pspmt
    R3292 02 Pspmt, supplied by Hamamatsu, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/r3292 02 pspmt/product/Hamamatsu
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    r3292 02 pspmt - by Bioz Stars, 2024-02
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    86
    Hamamatsu large position sensitive photomultiplier tubes
    Large Position Sensitive Photomultiplier Tubes, supplied by Hamamatsu, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/large position sensitive photomultiplier tubes/product/Hamamatsu
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    large position sensitive photomultiplier tubes - by Bioz Stars, 2024-02
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    86
    Millipore slc26a3
    Human colon epithelium is composed by a heterogeneous population of cells, which differ in expression of multiple markers, based on their lineage (A, goblet cells express MUC2), differentiation stage (B, “top-of-the-crypt” cells express high levels of KRT20) and proliferative status (C, proliferating cells express Ki67). In the normal colon epithelium, “top-of-the-crypt” and “bottom-of-the-crypt” epithelial cells can be differentially enriched by flow cytometry based of the expression of EpCAM, CD44 and CD66a/CEACAM1 (D–F). “Bottom of the crypt” epithelial cells were defined as EpCAM + /CD44 + (F, P12 blue sort gate) and “top-of-the-crypt” epithelial cells as EpCAM + /CD44 neg /CD66a high (F, P11 orange sort gate). SINCE-PCR analysis of “top-of-the-crypt” and “bottom-of-the-crypt” normal colon epithelial cells led to the discovery of novel lineage and/or differentiation markers and the establishment of a core set of 57 TaqMan assays that allow the visualization of distinct cell populations, including enterocyte-like cells (CA1 + <t>/SLC26A3</t> + and GUCA2B + ), goblet-like cells (MUC2 + /TFF3 high ) and two compartments defined by gene-expression profiles reminiscent of more immature progenitors (OLFM4 + /CA2 high and LGR5 + /ASCL2 + ) (I). CA1 + /SLC26A3 + and GUCA2B + cells were preferentially observed in the EpCAM + /CD44 neg /CD66a high population (P11 orange sort gate), while MUC2 + /TFF3 high , OLFM4 + /CA2 high and LGR5 + /ASCL2 + cells were preferentially observed in the EpCAM + /CD44 + population (P12 blue sort gate). Principal component analysis (PCA) of SINCE-PCR data confirmed hierarchical clustering results, visualizing distinct cell populations characterized by the coordinated expression of independent sets of genes (G–H; PC1: principal component #1, PC2: principal component #2). Both cell populations and gene families identified by PCA closely mirrored those identified by hierarchical clustering.
    Slc26a3, supplied by Millipore, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/slc26a3/product/Millipore
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    slc26a3 - by Bioz Stars, 2024-02
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    86
    Hamamatsu t ns jun 98 1138 1143 r3292 hamamatsu pspmt
    Human colon epithelium is composed by a heterogeneous population of cells, which differ in expression of multiple markers, based on their lineage (A, goblet cells express MUC2), differentiation stage (B, “top-of-the-crypt” cells express high levels of KRT20) and proliferative status (C, proliferating cells express Ki67). In the normal colon epithelium, “top-of-the-crypt” and “bottom-of-the-crypt” epithelial cells can be differentially enriched by flow cytometry based of the expression of EpCAM, CD44 and CD66a/CEACAM1 (D–F). “Bottom of the crypt” epithelial cells were defined as EpCAM + /CD44 + (F, P12 blue sort gate) and “top-of-the-crypt” epithelial cells as EpCAM + /CD44 neg /CD66a high (F, P11 orange sort gate). SINCE-PCR analysis of “top-of-the-crypt” and “bottom-of-the-crypt” normal colon epithelial cells led to the discovery of novel lineage and/or differentiation markers and the establishment of a core set of 57 TaqMan assays that allow the visualization of distinct cell populations, including enterocyte-like cells (CA1 + <t>/SLC26A3</t> + and GUCA2B + ), goblet-like cells (MUC2 + /TFF3 high ) and two compartments defined by gene-expression profiles reminiscent of more immature progenitors (OLFM4 + /CA2 high and LGR5 + /ASCL2 + ) (I). CA1 + /SLC26A3 + and GUCA2B + cells were preferentially observed in the EpCAM + /CD44 neg /CD66a high population (P11 orange sort gate), while MUC2 + /TFF3 high , OLFM4 + /CA2 high and LGR5 + /ASCL2 + cells were preferentially observed in the EpCAM + /CD44 + population (P12 blue sort gate). Principal component analysis (PCA) of SINCE-PCR data confirmed hierarchical clustering results, visualizing distinct cell populations characterized by the coordinated expression of independent sets of genes (G–H; PC1: principal component #1, PC2: principal component #2). Both cell populations and gene families identified by PCA closely mirrored those identified by hierarchical clustering.
    T Ns Jun 98 1138 1143 R3292 Hamamatsu Pspmt, supplied by Hamamatsu, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/t ns jun 98 1138 1143 r3292 hamamatsu pspmt/product/Hamamatsu
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    t ns jun 98 1138 1143 r3292 hamamatsu pspmt - by Bioz Stars, 2024-02
    86/100 stars
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    Image Search Results


    Human colon epithelium is composed by a heterogeneous population of cells, which differ in expression of multiple markers, based on their lineage (A, goblet cells express MUC2), differentiation stage (B, “top-of-the-crypt” cells express high levels of KRT20) and proliferative status (C, proliferating cells express Ki67). In the normal colon epithelium, “top-of-the-crypt” and “bottom-of-the-crypt” epithelial cells can be differentially enriched by flow cytometry based of the expression of EpCAM, CD44 and CD66a/CEACAM1 (D–F). “Bottom of the crypt” epithelial cells were defined as EpCAM + /CD44 + (F, P12 blue sort gate) and “top-of-the-crypt” epithelial cells as EpCAM + /CD44 neg /CD66a high (F, P11 orange sort gate). SINCE-PCR analysis of “top-of-the-crypt” and “bottom-of-the-crypt” normal colon epithelial cells led to the discovery of novel lineage and/or differentiation markers and the establishment of a core set of 57 TaqMan assays that allow the visualization of distinct cell populations, including enterocyte-like cells (CA1 + /SLC26A3 + and GUCA2B + ), goblet-like cells (MUC2 + /TFF3 high ) and two compartments defined by gene-expression profiles reminiscent of more immature progenitors (OLFM4 + /CA2 high and LGR5 + /ASCL2 + ) (I). CA1 + /SLC26A3 + and GUCA2B + cells were preferentially observed in the EpCAM + /CD44 neg /CD66a high population (P11 orange sort gate), while MUC2 + /TFF3 high , OLFM4 + /CA2 high and LGR5 + /ASCL2 + cells were preferentially observed in the EpCAM + /CD44 + population (P12 blue sort gate). Principal component analysis (PCA) of SINCE-PCR data confirmed hierarchical clustering results, visualizing distinct cell populations characterized by the coordinated expression of independent sets of genes (G–H; PC1: principal component #1, PC2: principal component #2). Both cell populations and gene families identified by PCA closely mirrored those identified by hierarchical clustering.

    Journal: Nature biotechnology

    Article Title: Single-cell dissection of transcriptional heterogeneity in human colon tumors

    doi: 10.1038/nbt.2038

    Figure Lengend Snippet: Human colon epithelium is composed by a heterogeneous population of cells, which differ in expression of multiple markers, based on their lineage (A, goblet cells express MUC2), differentiation stage (B, “top-of-the-crypt” cells express high levels of KRT20) and proliferative status (C, proliferating cells express Ki67). In the normal colon epithelium, “top-of-the-crypt” and “bottom-of-the-crypt” epithelial cells can be differentially enriched by flow cytometry based of the expression of EpCAM, CD44 and CD66a/CEACAM1 (D–F). “Bottom of the crypt” epithelial cells were defined as EpCAM + /CD44 + (F, P12 blue sort gate) and “top-of-the-crypt” epithelial cells as EpCAM + /CD44 neg /CD66a high (F, P11 orange sort gate). SINCE-PCR analysis of “top-of-the-crypt” and “bottom-of-the-crypt” normal colon epithelial cells led to the discovery of novel lineage and/or differentiation markers and the establishment of a core set of 57 TaqMan assays that allow the visualization of distinct cell populations, including enterocyte-like cells (CA1 + /SLC26A3 + and GUCA2B + ), goblet-like cells (MUC2 + /TFF3 high ) and two compartments defined by gene-expression profiles reminiscent of more immature progenitors (OLFM4 + /CA2 high and LGR5 + /ASCL2 + ) (I). CA1 + /SLC26A3 + and GUCA2B + cells were preferentially observed in the EpCAM + /CD44 neg /CD66a high population (P11 orange sort gate), while MUC2 + /TFF3 high , OLFM4 + /CA2 high and LGR5 + /ASCL2 + cells were preferentially observed in the EpCAM + /CD44 + population (P12 blue sort gate). Principal component analysis (PCA) of SINCE-PCR data confirmed hierarchical clustering results, visualizing distinct cell populations characterized by the coordinated expression of independent sets of genes (G–H; PC1: principal component #1, PC2: principal component #2). Both cell populations and gene families identified by PCA closely mirrored those identified by hierarchical clustering.

    Article Snippet: Paraffin-embedded tissue sections were stained with anti-human CK20 (clone Ks20.8, DakoCytomation), MUC2 (clone Ccp58, Fitzgerald Industries), Ki67 (clone MIB-1, DakoCytomation), CEACAM1/CD66a (clone 283340; R&D Systems) and SLC26A3 (lot #R32905, Sigma Life Science) antibodies, according to manufacturers’ instructions.

    Techniques: Expressing, Flow Cytometry

    To evaluate whether genes identified by SINCE-PCR as differentially expressed during normal colon differentiation can be used as prognostic markers for colon cancer patients, we analyzed a pooled database of 299 primary colon cancer gene-expression arrays annotated with disease-free survival (DFS) data (Jorissen and Smith, ). First, we used the Hegemon software to graph individual arrays according to the expression levels of KRT20 and one of four genes characteristic of “top-of-the-crypt” CA1 + /SLC26A3 + enterocyte-like cells (A, CA1; D, MS4A12; G, CD177; J, SLC26A3) and we exploited the StepMiner algorithm to define gene-expression thresholds. In all four instances, three distinct gene-expression groups could be visualized: Group 1 (green), defined as KRT20 + /CA1 high , KRT20 + /MS4A12 high , KRT20 + /CD177 + or KRT20 + /SLC26A3 + , respectively; Group 2 (blue), defined as KRT20 + /CA1 neg/low , KRT20 + /MS4A12 neg/low , KRT20 + /CD177 neg or KRT20 + /SLC26A3 neg , respectively; Group 3 (red), defined as KRT20 neg /CA1 neg/low , KRT20 neg /MS4A12 neg/low , KRT20 neg /CD177 neg or KRT20 neg /SLC26A3 neg , respectively. In all instances, an increasingly immature gene-expression profile corresponded to a progressively worse prognosis (B, F, H, K). Multivariate analysis of survival data indicated that the prognostic effect of these “gene-expression groups” is not confounded by clinical stage, age or sex (C, F, I, L; * p < 0.05, ** p < 0.001).

    Journal: Nature biotechnology

    Article Title: Single-cell dissection of transcriptional heterogeneity in human colon tumors

    doi: 10.1038/nbt.2038

    Figure Lengend Snippet: To evaluate whether genes identified by SINCE-PCR as differentially expressed during normal colon differentiation can be used as prognostic markers for colon cancer patients, we analyzed a pooled database of 299 primary colon cancer gene-expression arrays annotated with disease-free survival (DFS) data (Jorissen and Smith, ). First, we used the Hegemon software to graph individual arrays according to the expression levels of KRT20 and one of four genes characteristic of “top-of-the-crypt” CA1 + /SLC26A3 + enterocyte-like cells (A, CA1; D, MS4A12; G, CD177; J, SLC26A3) and we exploited the StepMiner algorithm to define gene-expression thresholds. In all four instances, three distinct gene-expression groups could be visualized: Group 1 (green), defined as KRT20 + /CA1 high , KRT20 + /MS4A12 high , KRT20 + /CD177 + or KRT20 + /SLC26A3 + , respectively; Group 2 (blue), defined as KRT20 + /CA1 neg/low , KRT20 + /MS4A12 neg/low , KRT20 + /CD177 neg or KRT20 + /SLC26A3 neg , respectively; Group 3 (red), defined as KRT20 neg /CA1 neg/low , KRT20 neg /MS4A12 neg/low , KRT20 neg /CD177 neg or KRT20 neg /SLC26A3 neg , respectively. In all instances, an increasingly immature gene-expression profile corresponded to a progressively worse prognosis (B, F, H, K). Multivariate analysis of survival data indicated that the prognostic effect of these “gene-expression groups” is not confounded by clinical stage, age or sex (C, F, I, L; * p < 0.05, ** p < 0.001).

    Article Snippet: Paraffin-embedded tissue sections were stained with anti-human CK20 (clone Ks20.8, DakoCytomation), MUC2 (clone Ccp58, Fitzgerald Industries), Ki67 (clone MIB-1, DakoCytomation), CEACAM1/CD66a (clone 283340; R&D Systems) and SLC26A3 (lot #R32905, Sigma Life Science) antibodies, according to manufacturers’ instructions.

    Techniques: Expressing, Software

    A direct comparison of the prognostic effect of gene-expression groups identified based on the “KRT20/top-crypt” two-gene scoring system with that of traditional pathological grading, using multivariate analysis based on the Cox proportional hazards model, was performed on a subset database of 181 microarrays annotated with grading information (Smith database, n=181, see ). The analysis indicated that the prognostic effect of “KRT20/top-crypt” gene-expression groups is not confounded by and is associated to higher hazard-ratios (HR) as compared to traditional pathological grade, independently of the gene chosen as marker of the “top-of-the-crypt” CA1 + /SLC26A3 + enterocyte-type cell population, with the only exception of CD177 (A, CA1; B, MS4A12; C, CD177; D, SLC26A3; * p-value < 0.05, ** p-value < 0.001).

    Journal: Nature biotechnology

    Article Title: Single-cell dissection of transcriptional heterogeneity in human colon tumors

    doi: 10.1038/nbt.2038

    Figure Lengend Snippet: A direct comparison of the prognostic effect of gene-expression groups identified based on the “KRT20/top-crypt” two-gene scoring system with that of traditional pathological grading, using multivariate analysis based on the Cox proportional hazards model, was performed on a subset database of 181 microarrays annotated with grading information (Smith database, n=181, see ). The analysis indicated that the prognostic effect of “KRT20/top-crypt” gene-expression groups is not confounded by and is associated to higher hazard-ratios (HR) as compared to traditional pathological grade, independently of the gene chosen as marker of the “top-of-the-crypt” CA1 + /SLC26A3 + enterocyte-type cell population, with the only exception of CD177 (A, CA1; B, MS4A12; C, CD177; D, SLC26A3; * p-value < 0.05, ** p-value < 0.001).

    Article Snippet: Paraffin-embedded tissue sections were stained with anti-human CK20 (clone Ks20.8, DakoCytomation), MUC2 (clone Ccp58, Fitzgerald Industries), Ki67 (clone MIB-1, DakoCytomation), CEACAM1/CD66a (clone 283340; R&D Systems) and SLC26A3 (lot #R32905, Sigma Life Science) antibodies, according to manufacturers’ instructions.

    Techniques: Expressing, Marker