R31890 Search Results


  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 96
    New England Biolabs noti-hf
    Noti Hf, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/noti-hf/product/New England Biolabs
    Average 96 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    noti-hf - by Bioz Stars, 2024-05
    96/100 stars
      Buy from Supplier

    86
    Thermo Fisher r3189s xbai invitrogen
    KEY RESOURCES TABLE
    R3189s Xbai Invitrogen, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/r3189s xbai invitrogen/product/Thermo Fisher
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    r3189s xbai invitrogen - by Bioz Stars, 2024-05
    86/100 stars
      Buy from Supplier

    86
    Millipore r3189s np40 sigma aldrich
    KEY RESOURCES TABLE
    R3189s Np40 Sigma Aldrich, supplied by Millipore, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/r3189s np40 sigma aldrich/product/Millipore
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    r3189s np40 sigma aldrich - by Bioz Stars, 2024-05
    86/100 stars
      Buy from Supplier

    86
    JSR Corporation material
    KEY RESOURCES TABLE
    Material, supplied by JSR Corporation, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/material/product/JSR Corporation
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    material - by Bioz Stars, 2024-05
    86/100 stars
      Buy from Supplier

    86
    Cell Signaling Technology Inc noti
    KEY RESOURCES TABLE
    Noti, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/noti/product/Cell Signaling Technology Inc
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    noti - by Bioz Stars, 2024-05
    86/100 stars
      Buy from Supplier

    Image Search Results


    KEY RESOURCES TABLE

    Journal: Molecular cell

    Article Title: POLE mutation spectra are shaped by the mutant allele identity, its abundance and mismatch repair status

    doi: 10.1016/j.molcel.2020.05.012

    Figure Lengend Snippet: KEY RESOURCES TABLE

    Article Snippet: ​ REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Goat anti-mouse IgG Sigma-Aldrich Cat# F0257; RRID:AB_259378 Purified anti-BrdU Biolegend Cat# 317902; RRID:AB_604040 Phospho-p53 (Ser15) mouse Cell Signaling Technology Cat# 9286; RRID:AB_331741 Anti-p53 (DO-1) Santa Cruz Biotechnology Cat# SC-126; RRID:AB_628082 Anti-p21 Abcam Cat# CP74; RRID:AB_1603138 Anti-MDM2 (SMP14) Santa Cruz Biotechnology Cat# SC-965; RRID:AB_627920 Anti-PUMAα/β (H-136) Santa Cruz Biotechnology Cat# SC-28226; RRID:AB_2064827 Bacterial and Virus Strains Subcloning Efficiency DH5α Competent Cells Invitrogen Cat# 18265017 Ad-Cre-GFP Adenovirus, Cre Recombinase Vector Biolabs Cat# 1700 Biological Samples N/A Chemicals, Peptides, and Recombinant Proteins UltraPure Phenol:Chloroform:Isoamyl Alcohol (25:24:1, v/v) Invitrogen Cat# 15593031 KpnI-HF New England Biolabs Cat# R3142S SacI-HF New England Biolabs Cat# R3138S EcoRI-HF New England Biolabs Cat# R3101S NotI-HF New England Biolabs Cat# R3189S XbaI Invitrogen Cat# IVGN0126 T7 Endonuclease I New England Biolabs Cat# M0302S Lipofectamine 2000 Invitrogen Cat# 11668030 Geneticin Life Technologies Cat# 10131027 HAT Supplement (50X) Life Technologies Cat# 21060017 6-Thioguanine Sigma Cat# A4882-250MG Crystal Violet ThermoFisher Scientific Cat# C581-100 MEM Non-Essential Amino Acids Solution (100X) Life Technologies Cat# 11140050 0.05% Trypsin-EDTA Life Technologies Cat# 25300120 Trizol Reagent Invitrogen Cat# 15596018 DEPC-Treated Water Invitrogen Cat# AM9915G 5-Bromo-2′-deoxyuridine Sigma Cat# B5002 Propidium iodide Sigma Cat# P4170 SuperSignal™ West Pico PLUS Chemiluminescent Substrate ThermoFisher Scientific Cat# 34578 Critical Commercial Assays SsoAdvanced Universal SYBR Green Supermix Bio-Rad Cat# 1725270 PureLink PCR Purification Kit Invitrogen Cat# K310002 GeneArt CRISPR Nuclease Vector with OFP Reporter Kit Invitrogen Cat# A21174 TOPO TA Cloning Kit for Subcloning Invitrogen Cat# 451641 First-Strand cDNA Synthesis Kit GE Healthcare Life Sciences Cat# 27926101 Bio-Rad Protein Assay Dye Reagent Concentrate Bio-Rad Cat# 5000006 Deposited Data Genomic Data Commons Data Portal National Cancer Institute GDC Data Portal https://portal.gdc.cancer.gov/ Whole Exome Sequencing Raw Datasets This paper SRA accession number: PRJNA589337 Unprocessed Western blot images Mendeley doi: 10.17632/cdgs6fmpsn.2 Experimental Models: Cell Lines Human: HCT116 Other RRID: CVCL_0291 Human: HCT116+Mlh1 This paper (also Hodel et al 2018 eLife) N/A Human: HCT116 POLE wt/S459F This paper N/A Human: HCT116 POLE wt/S459F +Mlh1 This paper N/A Human: HCT116 POLE S459F/S459F +Mlh1 This paper N/A Human: HCT116 POLE wt/P286R +Mlh1 This paper N/A Experimental Models: Organisms/Strains N/A Oligonucleotides POLE guide sequences, see Supp.

    Techniques: Purification, Subcloning, Plasmid Preparation, Recombinant, SYBR Green Assay, CRISPR, TA Cloning, Sequencing, Western Blot, Variant Assay, Software