Journal: BMC Plant Biology
Article Title: Simultaneous induction of mutant alleles of two allergenic genes in soybean by using site-directed mutagenesis
Figure Lengend Snippet: Confirmation of mutagenesis of targeted loci in representative Enrei-T 1 plants by CAPS analysis. The schematic diagrams above panels show DdeI or BsaJI restriction sites (shaded in gray) in fragments amplified with specific primers. Red and blue nucleotide sequences have the same meaning as those in Fig. 1 . Gray arrows, the sizes of expected wild-type fragments; black arrows, the sizes of expected mutant-type fragments; open triangle, a fragment of unexpected size considered as mutant type. M, molecular weight marker (100-bp ladder)
Article Snippet: The PCR was performed under the following conditions: 30 cycles of 94 °C for 30 s, 54 °C (the Gly m Bd 28 K ) or 60 °C (the Gly m Bd 30 K) for 30 s and 72 °C for 60 s. The amplified products were digested with the DdeI and BsaJI restriction enzymes (NEB), respectively, and separated by electrophoresis in 2.0% agarose gels.
Techniques: Mutagenesis, Amplification, Molecular Weight, Marker