R0505 Search Results


  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 94
    Elabscience hmgb1 enzyme linked immunosorbent assay elisa kit
    Effects of exogeneous recombinant <t>HMGB1</t> and CM on function of glutamate clearance in primary astrocytes. ( A ) primary astrocytes were treated with rHMGB1 10–40 ng/mL, and the activity of glutamate clearance was assayed 24 h later. ( B ) primary astrocytes were incubated with 4 ng/mL rHMGB1 for 24 h, and GLAST protein was examined using Western blotting. ( C ) CM was collected from OGD/R-treated astrocytic culture and transferred to primary astrocytes to incubate for 12–48 h. Thereafter, the activity of glutamate clearance was assayed at each indicated time point. ( D ) Expression of GLAST protein in CM-treated astrocytes was assayed at each indicated time point by Western blotting. ( E ) Primary astrocytes were exposed to CM with or without glycyrrhizic acid (25–200 μM) and the activity of astrocytic glutamate clearance was assayed 24 h later. * p
    Hmgb1 Enzyme Linked Immunosorbent Assay Elisa Kit, supplied by Elabscience, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/hmgb1 enzyme linked immunosorbent assay elisa kit/product/Elabscience
    Average 94 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    hmgb1 enzyme linked immunosorbent assay elisa kit - by Bioz Stars, 2022-07
    94/100 stars
      Buy from Supplier

    93
    New England Biolabs eagi
    Effects of exogeneous recombinant <t>HMGB1</t> and CM on function of glutamate clearance in primary astrocytes. ( A ) primary astrocytes were treated with rHMGB1 10–40 ng/mL, and the activity of glutamate clearance was assayed 24 h later. ( B ) primary astrocytes were incubated with 4 ng/mL rHMGB1 for 24 h, and GLAST protein was examined using Western blotting. ( C ) CM was collected from OGD/R-treated astrocytic culture and transferred to primary astrocytes to incubate for 12–48 h. Thereafter, the activity of glutamate clearance was assayed at each indicated time point. ( D ) Expression of GLAST protein in CM-treated astrocytes was assayed at each indicated time point by Western blotting. ( E ) Primary astrocytes were exposed to CM with or without glycyrrhizic acid (25–200 μM) and the activity of astrocytic glutamate clearance was assayed 24 h later. * p
    Eagi, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/eagi/product/New England Biolabs
    Average 93 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    eagi - by Bioz Stars, 2022-07
    93/100 stars
      Buy from Supplier

    Image Search Results


    Effects of exogeneous recombinant HMGB1 and CM on function of glutamate clearance in primary astrocytes. ( A ) primary astrocytes were treated with rHMGB1 10–40 ng/mL, and the activity of glutamate clearance was assayed 24 h later. ( B ) primary astrocytes were incubated with 4 ng/mL rHMGB1 for 24 h, and GLAST protein was examined using Western blotting. ( C ) CM was collected from OGD/R-treated astrocytic culture and transferred to primary astrocytes to incubate for 12–48 h. Thereafter, the activity of glutamate clearance was assayed at each indicated time point. ( D ) Expression of GLAST protein in CM-treated astrocytes was assayed at each indicated time point by Western blotting. ( E ) Primary astrocytes were exposed to CM with or without glycyrrhizic acid (25–200 μM) and the activity of astrocytic glutamate clearance was assayed 24 h later. * p

    Journal: Cells

    Article Title: Role of HMGB1/TLR4 Axis in Ischemia/Reperfusion-Impaired Extracellular Glutamate Clearance in Primary Astrocytes

    doi: 10.3390/cells9122585

    Figure Lengend Snippet: Effects of exogeneous recombinant HMGB1 and CM on function of glutamate clearance in primary astrocytes. ( A ) primary astrocytes were treated with rHMGB1 10–40 ng/mL, and the activity of glutamate clearance was assayed 24 h later. ( B ) primary astrocytes were incubated with 4 ng/mL rHMGB1 for 24 h, and GLAST protein was examined using Western blotting. ( C ) CM was collected from OGD/R-treated astrocytic culture and transferred to primary astrocytes to incubate for 12–48 h. Thereafter, the activity of glutamate clearance was assayed at each indicated time point. ( D ) Expression of GLAST protein in CM-treated astrocytes was assayed at each indicated time point by Western blotting. ( E ) Primary astrocytes were exposed to CM with or without glycyrrhizic acid (25–200 μM) and the activity of astrocytic glutamate clearance was assayed 24 h later. * p

    Article Snippet: Commercial kits, including HMGB1 ELISA (Elabscience Biotechnology Co., Shanghai, China), lactate dehydrogenase (LDH) release assay (Nanjing Jiancheng Bioengineering Institute, Nanjing, China), and Bio-Rad protein assay (Assay Kit II #5000002, Bio-Rad Laboratories, Feldkirchen, Germany), were used following the manufacturers’ instructions.

    Techniques: Recombinant, Activity Assay, Incubation, Western Blot, Expressing

    Effects of HMGB1 knockdown on OGD/R-impaired function of glutamate clearance in primary astrocytes. HMGB1 and control siRNA-treated astrocytes were exposed to OGD for 6 h followed by reoxygenation for 24 h. Thereafter, the expression of HMGB1 and GLAST protein ( A ) was examined using Western blot. Western blot of GLAST ( B ) was quantified using densitometry and ImageJ software. HMGB1 secretion ( C ) was analyzed using ELISA. The activity of glutamate clearance ( D ) was assayed at 24 h after reoxygenation. TLR4 and control siRNA-treated astrocytes were exposed to OGD/R insult. The expression of TLR4 ( E ) was examined by Western blot 24 h later. Activity of glutamate clearance in TLR4 and control siRNA-treated astrocytes was assayed at 24 h after reoxygenation ( F ). ** p

    Journal: Cells

    Article Title: Role of HMGB1/TLR4 Axis in Ischemia/Reperfusion-Impaired Extracellular Glutamate Clearance in Primary Astrocytes

    doi: 10.3390/cells9122585

    Figure Lengend Snippet: Effects of HMGB1 knockdown on OGD/R-impaired function of glutamate clearance in primary astrocytes. HMGB1 and control siRNA-treated astrocytes were exposed to OGD for 6 h followed by reoxygenation for 24 h. Thereafter, the expression of HMGB1 and GLAST protein ( A ) was examined using Western blot. Western blot of GLAST ( B ) was quantified using densitometry and ImageJ software. HMGB1 secretion ( C ) was analyzed using ELISA. The activity of glutamate clearance ( D ) was assayed at 24 h after reoxygenation. TLR4 and control siRNA-treated astrocytes were exposed to OGD/R insult. The expression of TLR4 ( E ) was examined by Western blot 24 h later. Activity of glutamate clearance in TLR4 and control siRNA-treated astrocytes was assayed at 24 h after reoxygenation ( F ). ** p

    Article Snippet: Commercial kits, including HMGB1 ELISA (Elabscience Biotechnology Co., Shanghai, China), lactate dehydrogenase (LDH) release assay (Nanjing Jiancheng Bioengineering Institute, Nanjing, China), and Bio-Rad protein assay (Assay Kit II #5000002, Bio-Rad Laboratories, Feldkirchen, Germany), were used following the manufacturers’ instructions.

    Techniques: Expressing, Western Blot, Software, Enzyme-linked Immunosorbent Assay, Activity Assay

    Effects of OGD/R on the expression of HMGB1 and TLR4 and the function of glutamate clearance in primary astrocytes. Primary astrocytes were exposed to OGD for 6 h followed by returning the cells to the normal cell culture condition (reoxygenation for 6–48 h). Thereafter, the expression of HMGB1, TLR4 and GLAST protein ( A ) were examined using Western blot. HMGB1 secretion ( B ) was analyzed using ELISA. The activity of glutamate clearance ( C ) was measured at each indicated time points after reoxygenation. Cell damage was assayed by measuring LDH release ( D ) * p

    Journal: Cells

    Article Title: Role of HMGB1/TLR4 Axis in Ischemia/Reperfusion-Impaired Extracellular Glutamate Clearance in Primary Astrocytes

    doi: 10.3390/cells9122585

    Figure Lengend Snippet: Effects of OGD/R on the expression of HMGB1 and TLR4 and the function of glutamate clearance in primary astrocytes. Primary astrocytes were exposed to OGD for 6 h followed by returning the cells to the normal cell culture condition (reoxygenation for 6–48 h). Thereafter, the expression of HMGB1, TLR4 and GLAST protein ( A ) were examined using Western blot. HMGB1 secretion ( B ) was analyzed using ELISA. The activity of glutamate clearance ( C ) was measured at each indicated time points after reoxygenation. Cell damage was assayed by measuring LDH release ( D ) * p

    Article Snippet: Commercial kits, including HMGB1 ELISA (Elabscience Biotechnology Co., Shanghai, China), lactate dehydrogenase (LDH) release assay (Nanjing Jiancheng Bioengineering Institute, Nanjing, China), and Bio-Rad protein assay (Assay Kit II #5000002, Bio-Rad Laboratories, Feldkirchen, Germany), were used following the manufacturers’ instructions.

    Techniques: Expressing, Cell Culture, Western Blot, Enzyme-linked Immunosorbent Assay, Activity Assay

    Role of TLR4 on HMGB1 and CM-impaired function of glutamate clearance in primary astrocytes. ( A ) Effects of TLR4 knockdown on rHMGB1-reduced expression of GLAST. TLR4 and control siRNA-treated astrocytes were exposed to rHMGB1 (40 ng/mL) for 24 h and the expression levels of GLAST were examined using Western blot. ( B ) Effects of TLR4 inhibitors on rHMGB1-inhibited activity of astrocytic glutamate clearance. Primary astrocytes were exposed to rHMGB1 (40 ng/mL) with or without TLR4 inhibitors, eritoran or LPS-RS, and the activity of glutamate clearance was assayed 24 h later. ( C ) Primary astrocytes were exposed to CM with or without TLR4 inhibitors, eritoran or LPS-RS, for 24 h. Thereafter, the expression levels of GLAST protein were examined using Western blot. ( D ) Activity of astrocytic glutamate clearance was assayed by measuring the residual glutamate in CM. ## p

    Journal: Cells

    Article Title: Role of HMGB1/TLR4 Axis in Ischemia/Reperfusion-Impaired Extracellular Glutamate Clearance in Primary Astrocytes

    doi: 10.3390/cells9122585

    Figure Lengend Snippet: Role of TLR4 on HMGB1 and CM-impaired function of glutamate clearance in primary astrocytes. ( A ) Effects of TLR4 knockdown on rHMGB1-reduced expression of GLAST. TLR4 and control siRNA-treated astrocytes were exposed to rHMGB1 (40 ng/mL) for 24 h and the expression levels of GLAST were examined using Western blot. ( B ) Effects of TLR4 inhibitors on rHMGB1-inhibited activity of astrocytic glutamate clearance. Primary astrocytes were exposed to rHMGB1 (40 ng/mL) with or without TLR4 inhibitors, eritoran or LPS-RS, and the activity of glutamate clearance was assayed 24 h later. ( C ) Primary astrocytes were exposed to CM with or without TLR4 inhibitors, eritoran or LPS-RS, for 24 h. Thereafter, the expression levels of GLAST protein were examined using Western blot. ( D ) Activity of astrocytic glutamate clearance was assayed by measuring the residual glutamate in CM. ## p

    Article Snippet: Commercial kits, including HMGB1 ELISA (Elabscience Biotechnology Co., Shanghai, China), lactate dehydrogenase (LDH) release assay (Nanjing Jiancheng Bioengineering Institute, Nanjing, China), and Bio-Rad protein assay (Assay Kit II #5000002, Bio-Rad Laboratories, Feldkirchen, Germany), were used following the manufacturers’ instructions.

    Techniques: Expressing, Western Blot, Activity Assay