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    Santa Cruz Biotechnology anti trpv1
    Anti Trpv1, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    95
    Millipore keratinocyte basal medium
    IL-17A/TNFα-mediated synergistic induction of IL-19 and IL-20 is regulated by a p38 MAPK-, NF-κB-, and JNK1/2-dependent mechanism. (A~D) Human <t>keratinocytes</t> were preincubated with the indicated inhibitors for 45 minutes before being stimulated with IL-17A (100 ng/ml), TNFα (10 ng/ml), or IL-17A combined with TNFα for 24 hours. (E~H) Human keratinocytes were transfected with specific siRNA as indicated. A non-targeting pool of siRNAs served as negative control (siCon). (A, B, E, G) IL-19 and IL-20 mRNA expression were measured by qPCR (n=4). RPLP0 was used for normalisation. (C, D, F, H) IL-19 and IL-20 protein levels were measured by ELISA (n=4). IL: interleukin, TNFα: tumour necrosis factor alpha. * Non-inhibited cells compared with inhibited cells; transfected cells against (sip38αβ), p65 (sip65), JNK1/2 (siJNK1/2) compared with control siRNA (siCon) (p
    Keratinocyte Basal Medium, supplied by Millipore, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Image Search Results


    IL-17A/TNFα-mediated synergistic induction of IL-19 and IL-20 is regulated by a p38 MAPK-, NF-κB-, and JNK1/2-dependent mechanism. (A~D) Human keratinocytes were preincubated with the indicated inhibitors for 45 minutes before being stimulated with IL-17A (100 ng/ml), TNFα (10 ng/ml), or IL-17A combined with TNFα for 24 hours. (E~H) Human keratinocytes were transfected with specific siRNA as indicated. A non-targeting pool of siRNAs served as negative control (siCon). (A, B, E, G) IL-19 and IL-20 mRNA expression were measured by qPCR (n=4). RPLP0 was used for normalisation. (C, D, F, H) IL-19 and IL-20 protein levels were measured by ELISA (n=4). IL: interleukin, TNFα: tumour necrosis factor alpha. * Non-inhibited cells compared with inhibited cells; transfected cells against (sip38αβ), p65 (sip65), JNK1/2 (siJNK1/2) compared with control siRNA (siCon) (p

    Journal: Annals of Dermatology

    Article Title: I-Kappa-B-Zeta Regulates Interleukin-17A/Tumor Necrosis Factor-Alpha Mediated Synergistic Induction of Interleukin-19 and Interleukin-20 in Humane Keratinocytes

    doi: 10.5021/ad.2021.33.2.122

    Figure Lengend Snippet: IL-17A/TNFα-mediated synergistic induction of IL-19 and IL-20 is regulated by a p38 MAPK-, NF-κB-, and JNK1/2-dependent mechanism. (A~D) Human keratinocytes were preincubated with the indicated inhibitors for 45 minutes before being stimulated with IL-17A (100 ng/ml), TNFα (10 ng/ml), or IL-17A combined with TNFα for 24 hours. (E~H) Human keratinocytes were transfected with specific siRNA as indicated. A non-targeting pool of siRNAs served as negative control (siCon). (A, B, E, G) IL-19 and IL-20 mRNA expression were measured by qPCR (n=4). RPLP0 was used for normalisation. (C, D, F, H) IL-19 and IL-20 protein levels were measured by ELISA (n=4). IL: interleukin, TNFα: tumour necrosis factor alpha. * Non-inhibited cells compared with inhibited cells; transfected cells against (sip38αβ), p65 (sip65), JNK1/2 (siJNK1/2) compared with control siRNA (siCon) (p

    Article Snippet: The medium was changed to keratinocyte basal medium without growth factors 24 hours prior to stimulation with IL-17A (100 ng/ml) and/or TNFα (10 ng/ml).

    Techniques: Transfection, Negative Control, Expressing, Real-time Polymerase Chain Reaction, Enzyme-linked Immunosorbent Assay

    IκBζ regulates IL-17A/TNFα-mediated synergistic induction of IL-19 and IL-20. Human keratinocytes were transfected with IκBζ siRNA (siIκBζ) or control siRNA (siCon) before stimulated with IL-17A (100 ng/ml), TNFα (10 ng/ml) or the combinations as indicated for 24 hours (n=4). (A, B) mRNA expression was analysed by qPCR, and RPLP0 was used as a reference gene for normalisation. (C, D) Protein level was analysed by ELISA (n=4). Horizontal lines represent medians. IL: interleukin, TNFα: tumour necrosis factor alpha, IκBζ: I-kappa-B-zeta. * p

    Journal: Annals of Dermatology

    Article Title: I-Kappa-B-Zeta Regulates Interleukin-17A/Tumor Necrosis Factor-Alpha Mediated Synergistic Induction of Interleukin-19 and Interleukin-20 in Humane Keratinocytes

    doi: 10.5021/ad.2021.33.2.122

    Figure Lengend Snippet: IκBζ regulates IL-17A/TNFα-mediated synergistic induction of IL-19 and IL-20. Human keratinocytes were transfected with IκBζ siRNA (siIκBζ) or control siRNA (siCon) before stimulated with IL-17A (100 ng/ml), TNFα (10 ng/ml) or the combinations as indicated for 24 hours (n=4). (A, B) mRNA expression was analysed by qPCR, and RPLP0 was used as a reference gene for normalisation. (C, D) Protein level was analysed by ELISA (n=4). Horizontal lines represent medians. IL: interleukin, TNFα: tumour necrosis factor alpha, IκBζ: I-kappa-B-zeta. * p

    Article Snippet: The medium was changed to keratinocyte basal medium without growth factors 24 hours prior to stimulation with IL-17A (100 ng/ml) and/or TNFα (10 ng/ml).

    Techniques: Transfection, Expressing, Real-time Polymerase Chain Reaction, Enzyme-linked Immunosorbent Assay

    IL-19 and IL-20 mRNA and protein expression are synergistically induced by IL-17A and TNFα. Cultured human keratinocytes were stimulated with IL-17A (100 ng/ml), TNFα (10 ng/ml) or IL-17A combined with TNFα for 2, 6, 12, 24, or 48 hours (n=4). (A, B) IL-19 and IL-20 mRNA expression were analysed by qPCR, and RPLP0 was used as a reference gene for normalisation. (C, D) Protein expression was analysed by ELISA. IL: interleukin, TNFα: tumour necrosis factor alpha. * p

    Journal: Annals of Dermatology

    Article Title: I-Kappa-B-Zeta Regulates Interleukin-17A/Tumor Necrosis Factor-Alpha Mediated Synergistic Induction of Interleukin-19 and Interleukin-20 in Humane Keratinocytes

    doi: 10.5021/ad.2021.33.2.122

    Figure Lengend Snippet: IL-19 and IL-20 mRNA and protein expression are synergistically induced by IL-17A and TNFα. Cultured human keratinocytes were stimulated with IL-17A (100 ng/ml), TNFα (10 ng/ml) or IL-17A combined with TNFα for 2, 6, 12, 24, or 48 hours (n=4). (A, B) IL-19 and IL-20 mRNA expression were analysed by qPCR, and RPLP0 was used as a reference gene for normalisation. (C, D) Protein expression was analysed by ELISA. IL: interleukin, TNFα: tumour necrosis factor alpha. * p

    Article Snippet: The medium was changed to keratinocyte basal medium without growth factors 24 hours prior to stimulation with IL-17A (100 ng/ml) and/or TNFα (10 ng/ml).

    Techniques: Expressing, Cell Culture, Real-time Polymerase Chain Reaction, Enzyme-linked Immunosorbent Assay

    IL-17A and TNFα increased the DNA binding activity of NF-κB. Human keratinocytes were stimulated with IL-17A (100 ng/ml) or TNFα (10 ng/ml) or their combination for one hour before the NF-κB DNA binding activity was analysed by electrophoretic mobility shift assay. Antibodies against p50 and p65 were tested for their ability to cause a super shift of the nuclear complex associated with the probe. A representative result of three separate experiments is shown. IL: interleukin, TNFα: tumour necrosis factor alpha.

    Journal: Annals of Dermatology

    Article Title: I-Kappa-B-Zeta Regulates Interleukin-17A/Tumor Necrosis Factor-Alpha Mediated Synergistic Induction of Interleukin-19 and Interleukin-20 in Humane Keratinocytes

    doi: 10.5021/ad.2021.33.2.122

    Figure Lengend Snippet: IL-17A and TNFα increased the DNA binding activity of NF-κB. Human keratinocytes were stimulated with IL-17A (100 ng/ml) or TNFα (10 ng/ml) or their combination for one hour before the NF-κB DNA binding activity was analysed by electrophoretic mobility shift assay. Antibodies against p50 and p65 were tested for their ability to cause a super shift of the nuclear complex associated with the probe. A representative result of three separate experiments is shown. IL: interleukin, TNFα: tumour necrosis factor alpha.

    Article Snippet: The medium was changed to keratinocyte basal medium without growth factors 24 hours prior to stimulation with IL-17A (100 ng/ml) and/or TNFα (10 ng/ml).

    Techniques: Binding Assay, Activity Assay, Electrophoretic Mobility Shift Assay

    Basal keratinocytes migrate as a sheet between two static layers. (A) Measurements of cell areas (μm 2 ) of basal cells based on E-cad-CFP expression in different distances towards the wound, directly after wounding (0), 8, 16, and 24 h post wounding. (n = 2 individual mice, with four wounds each per time point). (B) Measurements of cell areas of basal (B, magenta) and suprabasal cells (SB, yellow), directly after wounding (0), 8, 16 and 24 h post wounding. (n = 2 individual mice, with four wounds each per time point). (C) Schematic representation of three proposed models of modes of migration during re-epithelization. (D) Representative sequential images of intravital microscopy of the suprabasal layer (top panel) and the basal layer (bottom panel) after scratch wounding. Scale bar, 100 μm. See also Video 1 . (E) Representative images of intravital microscopy of a defined area in proximity (290 μm away from the wound edge) to the wound bed starting 16 h after wound induction; suprabasal keratinocytes (top panel) and basal keratinocytes (bottom layer). Individual basal keratinocytes highlighted with a yellow outline. Scale bar 100 μm. See also Video 2 . (F) Quantification of mean migration velocity in relation to cell size 16 h after induction of scratch wounding. (n = 5 individual mice). Dashed line indicating 380 μm 2 , separating basal from suprabasal keratinocytes. (G) Quantification of mean migration velocity of migratory ( > 10 μm/8 h) basal keratinocytes in relation to the distance of the wound 16 h after induction of scratch wounding. Median and interquartile distances are plotted for the distance bins

    Journal: Life Science Alliance

    Article Title: Scratch-induced partial skin wounds re-epithelialize by sheets of independently migrating keratinocytes

    doi: 10.26508/lsa.202000765

    Figure Lengend Snippet: Basal keratinocytes migrate as a sheet between two static layers. (A) Measurements of cell areas (μm 2 ) of basal cells based on E-cad-CFP expression in different distances towards the wound, directly after wounding (0), 8, 16, and 24 h post wounding. (n = 2 individual mice, with four wounds each per time point). (B) Measurements of cell areas of basal (B, magenta) and suprabasal cells (SB, yellow), directly after wounding (0), 8, 16 and 24 h post wounding. (n = 2 individual mice, with four wounds each per time point). (C) Schematic representation of three proposed models of modes of migration during re-epithelization. (D) Representative sequential images of intravital microscopy of the suprabasal layer (top panel) and the basal layer (bottom panel) after scratch wounding. Scale bar, 100 μm. See also Video 1 . (E) Representative images of intravital microscopy of a defined area in proximity (290 μm away from the wound edge) to the wound bed starting 16 h after wound induction; suprabasal keratinocytes (top panel) and basal keratinocytes (bottom layer). Individual basal keratinocytes highlighted with a yellow outline. Scale bar 100 μm. See also Video 2 . (F) Quantification of mean migration velocity in relation to cell size 16 h after induction of scratch wounding. (n = 5 individual mice). Dashed line indicating 380 μm 2 , separating basal from suprabasal keratinocytes. (G) Quantification of mean migration velocity of migratory ( > 10 μm/8 h) basal keratinocytes in relation to the distance of the wound 16 h after induction of scratch wounding. Median and interquartile distances are plotted for the distance bins

    Article Snippet: Quantification was performed manually by counting the number of EdU-positive cells per total basal keratinocytes in the interfollicular epidermis.

    Techniques: Expressing, Mouse Assay, Migration, Intravital Microscopy

    Single keratinocytes move within the collectively migrating sheet. (A) Representative images of a migrating pairs at different distances to the scratch of recombined GFP-expressing cells in a R26-CreERT2:R26-mTmG mouse 16 h after scratch wound induction. Scale bar, 50 μm. (B) Reconstruction of migrating cell pairs and their neighboring cells, followed over minimal 2 h 30 min, 16 h after scratch wounding. The reconstructions depict three examples of cell group with different distances to the scratch wound. (C) The fraction of original neighbors that are remained is plotted over time. Basal keratinocytes are grouped based on initial distance to scratch wound ( > 1 mm; 0.5–1 mm;

    Journal: Life Science Alliance

    Article Title: Scratch-induced partial skin wounds re-epithelialize by sheets of independently migrating keratinocytes

    doi: 10.26508/lsa.202000765

    Figure Lengend Snippet: Single keratinocytes move within the collectively migrating sheet. (A) Representative images of a migrating pairs at different distances to the scratch of recombined GFP-expressing cells in a R26-CreERT2:R26-mTmG mouse 16 h after scratch wound induction. Scale bar, 50 μm. (B) Reconstruction of migrating cell pairs and their neighboring cells, followed over minimal 2 h 30 min, 16 h after scratch wounding. The reconstructions depict three examples of cell group with different distances to the scratch wound. (C) The fraction of original neighbors that are remained is plotted over time. Basal keratinocytes are grouped based on initial distance to scratch wound ( > 1 mm; 0.5–1 mm;

    Article Snippet: Quantification was performed manually by counting the number of EdU-positive cells per total basal keratinocytes in the interfollicular epidermis.

    Techniques: Expressing

    Migrating keratinocytes bypass hair follicle obstacles. (A) Representative images of migrating keratinocytes in a Fucci2 mouse 16 h after scratch wound induction bypassing a hair follicle (*). Scale bar, 50 μm. Individual keratinocytes are highlighted by their migration tracks. Also see Video 3 . (B) Representative sequential images of labelled cells within mTmG mice 16–24 h post wounding within the infundibulum and upper hair follicle (upper panel) and cells within the basal layer (lower panel). Migration/no migration is indicated by the shifts in yellow circles over time. (C) Quantification of total displacement in different distances towards the wound of hair follicle (grey) and basal cells (blue), respectively. Data points represent single cells from two mice, with five different wounds per mouse. **** P

    Journal: Life Science Alliance

    Article Title: Scratch-induced partial skin wounds re-epithelialize by sheets of independently migrating keratinocytes

    doi: 10.26508/lsa.202000765

    Figure Lengend Snippet: Migrating keratinocytes bypass hair follicle obstacles. (A) Representative images of migrating keratinocytes in a Fucci2 mouse 16 h after scratch wound induction bypassing a hair follicle (*). Scale bar, 50 μm. Individual keratinocytes are highlighted by their migration tracks. Also see Video 3 . (B) Representative sequential images of labelled cells within mTmG mice 16–24 h post wounding within the infundibulum and upper hair follicle (upper panel) and cells within the basal layer (lower panel). Migration/no migration is indicated by the shifts in yellow circles over time. (C) Quantification of total displacement in different distances towards the wound of hair follicle (grey) and basal cells (blue), respectively. Data points represent single cells from two mice, with five different wounds per mouse. **** P

    Article Snippet: Quantification was performed manually by counting the number of EdU-positive cells per total basal keratinocytes in the interfollicular epidermis.

    Techniques: Migration, Mouse Assay

    Velocity of exchanging neighboring cells. (A) The fraction of remaining original neighbors is plotted over time. Basal keratinocytes are grouped based on migration velocity (

    Journal: Life Science Alliance

    Article Title: Scratch-induced partial skin wounds re-epithelialize by sheets of independently migrating keratinocytes

    doi: 10.26508/lsa.202000765

    Figure Lengend Snippet: Velocity of exchanging neighboring cells. (A) The fraction of remaining original neighbors is plotted over time. Basal keratinocytes are grouped based on migration velocity (

    Article Snippet: Quantification was performed manually by counting the number of EdU-positive cells per total basal keratinocytes in the interfollicular epidermis.

    Techniques: Migration

    Proliferation occurs in a distinct zone and does not affect overall migration. (A) Left panel: fluorescence staining of EdU incorporation (green) immediately after wounding and 8, 16 and 24 h post wounding. LN332 (magenta) immunofluorescence counterstain marks the basement membrane. Scale bar 100 μm. Right panel: quantification of EdU incorporation, each dot represents one wound in n = 2 mice. (B) Representative image of a scratch wound in Fucci2 mice. Cells in G1-phase (magenta), in G2/S-phase (green) in their different distances towards the wound. Scale bar 100 μm. (C) Quantification of percentage of proliferating (G2/S, green) and non-proliferating cells (G1, magenta) grouped based on their distance to the wound (0–200, 200–400, 400–600, 600–800, and 800–1,000 μm). ** P = 0.0086 (ANOVA multiple comparison). (D) Percentage of proliferating G2/S cells within a distance of 200–400 μm away from the wound bed is plotted between 16 and 24 h post wounding. Plots depict the min., max., and median, the lower and upper quantile, the plus indicates the mean. (E) Linear regression analysis between scratch size (μm) and percentage of G2/S-phase proliferating cells 200–600 μm away from the wound site. R 2 = 0.8241. (F) Linear regression analysis between scratch size (μm) and average migration velocity (μm/h) of basal keratinocytes 0–200 μm away from the wound site. R 2 = 0.6129. (G) Quantification of migration velocity of proliferating S/G2-phase cells (green) and non-proliferating G1-phase cells (magenta) within different distances towards the wound. ns, not significant (unpaired t test). (H) Quantification of persistence of migratory proliferating S/G2-phase cells (green) and non-proliferating G1-phase cells (magenta) within different distances towards the wound. ns, not significant (Kruskal–Wallis test). Source data are available for this figure.

    Journal: Life Science Alliance

    Article Title: Scratch-induced partial skin wounds re-epithelialize by sheets of independently migrating keratinocytes

    doi: 10.26508/lsa.202000765

    Figure Lengend Snippet: Proliferation occurs in a distinct zone and does not affect overall migration. (A) Left panel: fluorescence staining of EdU incorporation (green) immediately after wounding and 8, 16 and 24 h post wounding. LN332 (magenta) immunofluorescence counterstain marks the basement membrane. Scale bar 100 μm. Right panel: quantification of EdU incorporation, each dot represents one wound in n = 2 mice. (B) Representative image of a scratch wound in Fucci2 mice. Cells in G1-phase (magenta), in G2/S-phase (green) in their different distances towards the wound. Scale bar 100 μm. (C) Quantification of percentage of proliferating (G2/S, green) and non-proliferating cells (G1, magenta) grouped based on their distance to the wound (0–200, 200–400, 400–600, 600–800, and 800–1,000 μm). ** P = 0.0086 (ANOVA multiple comparison). (D) Percentage of proliferating G2/S cells within a distance of 200–400 μm away from the wound bed is plotted between 16 and 24 h post wounding. Plots depict the min., max., and median, the lower and upper quantile, the plus indicates the mean. (E) Linear regression analysis between scratch size (μm) and percentage of G2/S-phase proliferating cells 200–600 μm away from the wound site. R 2 = 0.8241. (F) Linear regression analysis between scratch size (μm) and average migration velocity (μm/h) of basal keratinocytes 0–200 μm away from the wound site. R 2 = 0.6129. (G) Quantification of migration velocity of proliferating S/G2-phase cells (green) and non-proliferating G1-phase cells (magenta) within different distances towards the wound. ns, not significant (unpaired t test). (H) Quantification of persistence of migratory proliferating S/G2-phase cells (green) and non-proliferating G1-phase cells (magenta) within different distances towards the wound. ns, not significant (Kruskal–Wallis test). Source data are available for this figure.

    Article Snippet: Quantification was performed manually by counting the number of EdU-positive cells per total basal keratinocytes in the interfollicular epidermis.

    Techniques: Migration, Fluorescence, Staining, Immunofluorescence, Mouse Assay

    Model of scratch wound re-epithelialization. Schematic representation of the model for re-epithelization in partial-thickness wounds. Upon the initiation of a scratch wound, basal keratinocytes immediately move towards the wound side without entering the wound bed. After a period of jamming, basal keratinocytes enter the wound bed, whereas suprabasal keratinocytes remain static. Basal keratinocytes migrate individually through a collectively moving basal layer directed towards the wound bed. Left panel, top view of the basal migrating keratinocytes. Dashed line representing the cross section shown in the right panel.

    Journal: Life Science Alliance

    Article Title: Scratch-induced partial skin wounds re-epithelialize by sheets of independently migrating keratinocytes

    doi: 10.26508/lsa.202000765

    Figure Lengend Snippet: Model of scratch wound re-epithelialization. Schematic representation of the model for re-epithelization in partial-thickness wounds. Upon the initiation of a scratch wound, basal keratinocytes immediately move towards the wound side without entering the wound bed. After a period of jamming, basal keratinocytes enter the wound bed, whereas suprabasal keratinocytes remain static. Basal keratinocytes migrate individually through a collectively moving basal layer directed towards the wound bed. Left panel, top view of the basal migrating keratinocytes. Dashed line representing the cross section shown in the right panel.

    Article Snippet: Quantification was performed manually by counting the number of EdU-positive cells per total basal keratinocytes in the interfollicular epidermis.

    Techniques: