PKL Search Results


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  • 85
    Becton Dickinson anti git pkl
    Anti Git Pkl, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 85/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Becton Dickinson anti pkl
    Anti Pkl, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 99/100, based on 12 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Matrix Science pkl
    Pkl, supplied by Matrix Science, used in various techniques. Bioz Stars score: 88/100, based on 22 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    93
    Addgene inc addgene pkl ps113
    Identification of CDK as an Upstream Kinase for <t>PKL</t> <t>pS113</t> (A) Primary hepatocytes treated with glucose and the gluconeogenic precursors pyruvate and lactate showed increased PKL pS113 compared to just glucose or glucose with the PKA activator IBMX. ), revealing a CDK-like motif (+1P, +3–5 K/R). Residues shaded in gray are fixed, and the size of the residue correlates with the enrichment of that residue at a position. Residues taller than the red lines represent statistical significance (p
    Addgene Pkl Ps113, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    91
    AnaSpec fluorescently labeled camkiin peptide h krp pkl gqi gra krv vie ddr k hf488 nh2
    Identification of CDK as an Upstream Kinase for <t>PKL</t> <t>pS113</t> (A) Primary hepatocytes treated with glucose and the gluconeogenic precursors pyruvate and lactate showed increased PKL pS113 compared to just glucose or glucose with the PKA activator IBMX. ), revealing a CDK-like motif (+1P, +3–5 K/R). Residues shaded in gray are fixed, and the size of the residue correlates with the enrichment of that residue at a position. Residues taller than the red lines represent statistical significance (p
    Fluorescently Labeled Camkiin Peptide H Krp Pkl Gqi Gra Krv Vie Ddr K Hf488 Nh2, supplied by AnaSpec, used in various techniques. Bioz Stars score: 91/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    92
    Takeda ng pkl
    Identification of CDK as an Upstream Kinase for <t>PKL</t> <t>pS113</t> (A) Primary hepatocytes treated with glucose and the gluconeogenic precursors pyruvate and lactate showed increased PKL pS113 compared to just glucose or glucose with the PKA activator IBMX. ), revealing a CDK-like motif (+1P, +3–5 K/R). Residues shaded in gray are fixed, and the size of the residue correlates with the enrichment of that residue at a position. Residues taller than the red lines represent statistical significance (p
    Ng Pkl, supplied by Takeda, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    85
    Becton Dickinson p95 pkl monoclonal antibody
    Identification of CDK as an Upstream Kinase for <t>PKL</t> <t>pS113</t> (A) Primary hepatocytes treated with glucose and the gluconeogenic precursors pyruvate and lactate showed increased PKL pS113 compared to just glucose or glucose with the PKA activator IBMX. ), revealing a CDK-like motif (+1P, +3–5 K/R). Residues shaded in gray are fixed, and the size of the residue correlates with the enrichment of that residue at a position. Residues taller than the red lines represent statistical significance (p
    P95 Pkl Monoclonal Antibody, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 85/100, based on 9 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Matrix Science peak list files pkl
    Identification of CDK as an Upstream Kinase for <t>PKL</t> <t>pS113</t> (A) Primary hepatocytes treated with glucose and the gluconeogenic precursors pyruvate and lactate showed increased PKL pS113 compared to just glucose or glucose with the PKA activator IBMX. ), revealing a CDK-like motif (+1P, +3–5 K/R). Residues shaded in gray are fixed, and the size of the residue correlates with the enrichment of that residue at a position. Residues taller than the red lines represent statistical significance (p
    Peak List Files Pkl, supplied by Matrix Science, used in various techniques. Bioz Stars score: 85/100, based on 8 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    94
    Becton Dickinson pkl git1 antibody
    <t>PKL</t> is tyrosine-phosphorylated by Src and FAK in response to PDGF. (A) Endogenous PKL and <t>GIT1</t> were precipitated from quiescent and PDGF (20 ng/ml)-stimulated MEFs and blotted with phosphotyrosine antibody (PY, clone 4G10). (B) MEFs were transiently transfected
    Pkl Git1 Antibody, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 94/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    pkl  (Abcam)
    88
    Abcam pkl
    MiR-122 targeted <t>PKM2</t> and suppressed PKM2 expression. (A) Seed sequence of miR-122 in the 3UTR of PKM2 was underlined. WT and mutated sequences of PKM2 were inserted into pmiRGLO luciferase vector. (B) Re-expression of miR-122 suppressed luciferase activity of the WT but not the Mut 3UTR of PKM2. EV and sensor sequences served as negative and positive controls, respectively. (C) Re-expression of miR-122 in SMMC-7221 and MHCC-97L cells suppressed PKM2 but not <t>PKL/R</t> expression. (D) Linear regression model demonstrated that PKM2 mRNA expression was inversely correlated with miR-122 expression in HCC (pink) and NT liver tissues (blue). (E) Expression levels of 667 miRNA species in HCC and NT were plotted. Each dot represents one individual miRNA. MiR-122 is the most abundant miRNA in NT liver. (F) MiR-122 expression in NT, HCC, and venous metastases (VM). Data were retrieved from low density microarray in which expressions of 667 miRNA species were compared between NT, HCC, and VM. B , * P
    Pkl, supplied by Abcam, used in various techniques. Bioz Stars score: 88/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Becton Dickinson pkl
    In the absence of <t>FAK,</t> <t>PKL</t> and PAK activity are diminished at FAs. (A) Immunoblot analyses reveal reduced levels of PKL without major differences in levels of PAK or PAK-interacting guanine nucleotide exchange factor (βPIX; actin is control). (B) Immunofluorescence microscopy shows that PKL, βPIX, PAK, and phosphorylated (active) PAK (p-PAK) localize at FAs in WT MKs, whereas Fak KO FAs still contain βPIX but show severely reduced staining for PKL, PAK, and p-PAK. Phalloidin (red) marks F-actin, and DAPI (blue) marks chromatin.
    Pkl, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 88/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Waters Corporation pkl
    In the absence of <t>FAK,</t> <t>PKL</t> and PAK activity are diminished at FAs. (A) Immunoblot analyses reveal reduced levels of PKL without major differences in levels of PAK or PAK-interacting guanine nucleotide exchange factor (βPIX; actin is control). (B) Immunofluorescence microscopy shows that PKL, βPIX, PAK, and phosphorylated (active) PAK (p-PAK) localize at FAs in WT MKs, whereas Fak KO FAs still contain βPIX but show severely reduced staining for PKL, PAK, and p-PAK. Phalloidin (red) marks F-actin, and DAPI (blue) marks chromatin.
    Pkl, supplied by Waters Corporation, used in various techniques. Bioz Stars score: 88/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Image Search Results


    Identification of CDK as an Upstream Kinase for PKL pS113 (A) Primary hepatocytes treated with glucose and the gluconeogenic precursors pyruvate and lactate showed increased PKL pS113 compared to just glucose or glucose with the PKA activator IBMX. ), revealing a CDK-like motif (+1P, +3–5 K/R). Residues shaded in gray are fixed, and the size of the residue correlates with the enrichment of that residue at a position. Residues taller than the red lines represent statistical significance (p

    Journal: Cell reports

    Article Title: Distinct Hepatic PKA and CDK Signaling Pathways Control Activity-Independent Pyruvate Kinase Phosphorylation and Hepatic Glucose Production

    doi: 10.1016/j.celrep.2019.11.009

    Figure Lengend Snippet: Identification of CDK as an Upstream Kinase for PKL pS113 (A) Primary hepatocytes treated with glucose and the gluconeogenic precursors pyruvate and lactate showed increased PKL pS113 compared to just glucose or glucose with the PKA activator IBMX. ), revealing a CDK-like motif (+1P, +3–5 K/R). Residues shaded in gray are fixed, and the size of the residue correlates with the enrichment of that residue at a position. Residues taller than the red lines represent statistical significance (p

    Article Snippet: Plasmids generated in this study have been deposited to Addgene: PKL pS113 and PKL pS12 antibodies generated in this study will be made available on request but we may require a payment and/or a completed Materials Transfer Agreement if there is potential for commercial application.

    Techniques:

    PKL S113 Is Phosphorylated in the Nucleus, Leading to Nuclear Retention and a Distinct Pool of PKL from the Cytosolic pS12 Population (A and B) Rat hepatoma cells (A) and primary hepatocytes (B) were immunostained for PKL pS113 and show strong nuclear localization (4‘,6-diamidino-2-phenylindole [DAPI]) of the pS113 signal. Scale bar represents 50 μm. (C–E) Rat hepatoma cells (C), primary hepatocytes (D), and samples from control, HFD, and PKCε ASO rat liver lysates (E) were fractionated into nuclear and cytosolic fractions, and then blotted for pS113, pS12, total PKL, total PKM2, and Histone H3 (as a nuclear marker). (F) Nuclear and cytosolic fractions from rat hepatoma cells treated overnight with DMSO or 40 μM importazole. (G) Model of PKL translocation and phosphorylation.

    Journal: Cell reports

    Article Title: Distinct Hepatic PKA and CDK Signaling Pathways Control Activity-Independent Pyruvate Kinase Phosphorylation and Hepatic Glucose Production

    doi: 10.1016/j.celrep.2019.11.009

    Figure Lengend Snippet: PKL S113 Is Phosphorylated in the Nucleus, Leading to Nuclear Retention and a Distinct Pool of PKL from the Cytosolic pS12 Population (A and B) Rat hepatoma cells (A) and primary hepatocytes (B) were immunostained for PKL pS113 and show strong nuclear localization (4‘,6-diamidino-2-phenylindole [DAPI]) of the pS113 signal. Scale bar represents 50 μm. (C–E) Rat hepatoma cells (C), primary hepatocytes (D), and samples from control, HFD, and PKCε ASO rat liver lysates (E) were fractionated into nuclear and cytosolic fractions, and then blotted for pS113, pS12, total PKL, total PKM2, and Histone H3 (as a nuclear marker). (F) Nuclear and cytosolic fractions from rat hepatoma cells treated overnight with DMSO or 40 μM importazole. (G) Model of PKL translocation and phosphorylation.

    Article Snippet: Plasmids generated in this study have been deposited to Addgene: PKL pS113 and PKL pS12 antibodies generated in this study will be made available on request but we may require a payment and/or a completed Materials Transfer Agreement if there is potential for commercial application.

    Techniques: Allele-specific Oligonucleotide, Marker, Translocation Assay

    PKL is tyrosine-phosphorylated by Src and FAK in response to PDGF. (A) Endogenous PKL and GIT1 were precipitated from quiescent and PDGF (20 ng/ml)-stimulated MEFs and blotted with phosphotyrosine antibody (PY, clone 4G10). (B) MEFs were transiently transfected

    Journal: Molecular Biology of the Cell

    Article Title: Paxillin-Kinase-Linker Tyrosine Phosphorylation Regulates Directional Cell Migration

    doi: 10.1091/mbc.E09-07-0548

    Figure Lengend Snippet: PKL is tyrosine-phosphorylated by Src and FAK in response to PDGF. (A) Endogenous PKL and GIT1 were precipitated from quiescent and PDGF (20 ng/ml)-stimulated MEFs and blotted with phosphotyrosine antibody (PY, clone 4G10). (B) MEFs were transiently transfected

    Article Snippet: RNAi knockdown efficiency was determined by Western blotting with PKL/GIT1 antibody (BD Transduction Laboratories) and a PKL-specific antibody, kindly provided by Dr. Rick Horwitz (University of Virginia, Charlottesville, VA).

    Techniques: Transfection

    MiR-122 targeted PKM2 and suppressed PKM2 expression. (A) Seed sequence of miR-122 in the 3UTR of PKM2 was underlined. WT and mutated sequences of PKM2 were inserted into pmiRGLO luciferase vector. (B) Re-expression of miR-122 suppressed luciferase activity of the WT but not the Mut 3UTR of PKM2. EV and sensor sequences served as negative and positive controls, respectively. (C) Re-expression of miR-122 in SMMC-7221 and MHCC-97L cells suppressed PKM2 but not PKL/R expression. (D) Linear regression model demonstrated that PKM2 mRNA expression was inversely correlated with miR-122 expression in HCC (pink) and NT liver tissues (blue). (E) Expression levels of 667 miRNA species in HCC and NT were plotted. Each dot represents one individual miRNA. MiR-122 is the most abundant miRNA in NT liver. (F) MiR-122 expression in NT, HCC, and venous metastases (VM). Data were retrieved from low density microarray in which expressions of 667 miRNA species were compared between NT, HCC, and VM. B , * P

    Journal: PLoS ONE

    Article Title: Switching of Pyruvate Kinase Isoform L to M2 Promotes Metabolic Reprogramming in Hepatocarcinogenesis

    doi: 10.1371/journal.pone.0115036

    Figure Lengend Snippet: MiR-122 targeted PKM2 and suppressed PKM2 expression. (A) Seed sequence of miR-122 in the 3UTR of PKM2 was underlined. WT and mutated sequences of PKM2 were inserted into pmiRGLO luciferase vector. (B) Re-expression of miR-122 suppressed luciferase activity of the WT but not the Mut 3UTR of PKM2. EV and sensor sequences served as negative and positive controls, respectively. (C) Re-expression of miR-122 in SMMC-7221 and MHCC-97L cells suppressed PKM2 but not PKL/R expression. (D) Linear regression model demonstrated that PKM2 mRNA expression was inversely correlated with miR-122 expression in HCC (pink) and NT liver tissues (blue). (E) Expression levels of 667 miRNA species in HCC and NT were plotted. Each dot represents one individual miRNA. MiR-122 is the most abundant miRNA in NT liver. (F) MiR-122 expression in NT, HCC, and venous metastases (VM). Data were retrieved from low density microarray in which expressions of 667 miRNA species were compared between NT, HCC, and VM. B , * P

    Article Snippet: Antibodies, HCC tissue microarray sections, immunohistochemistry and histology PKM1 (Sigma), phospho-PKM2 (Tyr105) (Cell Signaling Technology, Danvers, MA), PKM2 (Cell Signaling Technology), PKL (Abcam), β actin (Sigma) were used for Western Blotting.

    Techniques: Expressing, Sequencing, Luciferase, Plasmid Preparation, Activity Assay, Microarray

    PKM2 expression in human HCC. (A) mRNA expression of PKL, PKM1, and PKM2 in HCC and NT tissues. Values = 2 ΔΔCT , ΔΔCT = (CT PK – CT HPRT ) of HCC - (CT PK – CT HPRT ) of NT. P values, Wilcoxin signed rank test (B) Waterfall plot shows that, at the mRNA level, PKM2 was up-regulated (HCC/NT2 folds) in 29/60 (48.33%) human HCC samples. (C) Representative pictures of IHC staining with antibody against PKM2 in HCC tissue microarray. PKM2 protein was drastically up-regulated in human HCCs as compared to the paired NT tissues. (D) Mann Whitney test showed that PKM2 over-expression was associated with multiple aggressive clinicopathological features in HCC including the presence of tumor microsatellites, presence of venous invasion, and absence of tumor encapsulation. (E) Over-expression of PKM2 in human HCC was associated with poor prognosis. HCC patients were categorized into two groups: PKM2 over-expression and PKM2 normal/under-expression. PKM2 was considered to be over-expressed when HCC/NT2 folds and was considered to be normal/under-expressed otherwise. HCC patients with PKM2 over-expression had a higher 1-year tumor recurrence rate after surgical resection than HCC patients without PKM2 over-expression, 46.667% Vs 25%. (F) Patients with PKM2 over-expression had lower 5-year overall survival rates after surgical resection. P values were calculated by Kaplan-Meir log rank test.

    Journal: PLoS ONE

    Article Title: Switching of Pyruvate Kinase Isoform L to M2 Promotes Metabolic Reprogramming in Hepatocarcinogenesis

    doi: 10.1371/journal.pone.0115036

    Figure Lengend Snippet: PKM2 expression in human HCC. (A) mRNA expression of PKL, PKM1, and PKM2 in HCC and NT tissues. Values = 2 ΔΔCT , ΔΔCT = (CT PK – CT HPRT ) of HCC - (CT PK – CT HPRT ) of NT. P values, Wilcoxin signed rank test (B) Waterfall plot shows that, at the mRNA level, PKM2 was up-regulated (HCC/NT2 folds) in 29/60 (48.33%) human HCC samples. (C) Representative pictures of IHC staining with antibody against PKM2 in HCC tissue microarray. PKM2 protein was drastically up-regulated in human HCCs as compared to the paired NT tissues. (D) Mann Whitney test showed that PKM2 over-expression was associated with multiple aggressive clinicopathological features in HCC including the presence of tumor microsatellites, presence of venous invasion, and absence of tumor encapsulation. (E) Over-expression of PKM2 in human HCC was associated with poor prognosis. HCC patients were categorized into two groups: PKM2 over-expression and PKM2 normal/under-expression. PKM2 was considered to be over-expressed when HCC/NT2 folds and was considered to be normal/under-expressed otherwise. HCC patients with PKM2 over-expression had a higher 1-year tumor recurrence rate after surgical resection than HCC patients without PKM2 over-expression, 46.667% Vs 25%. (F) Patients with PKM2 over-expression had lower 5-year overall survival rates after surgical resection. P values were calculated by Kaplan-Meir log rank test.

    Article Snippet: Antibodies, HCC tissue microarray sections, immunohistochemistry and histology PKM1 (Sigma), phospho-PKM2 (Tyr105) (Cell Signaling Technology, Danvers, MA), PKM2 (Cell Signaling Technology), PKL (Abcam), β actin (Sigma) were used for Western Blotting.

    Techniques: Expressing, Immunohistochemistry, Staining, Microarray, MANN-WHITNEY, Over Expression

    In the absence of FAK, PKL and PAK activity are diminished at FAs. (A) Immunoblot analyses reveal reduced levels of PKL without major differences in levels of PAK or PAK-interacting guanine nucleotide exchange factor (βPIX; actin is control). (B) Immunofluorescence microscopy shows that PKL, βPIX, PAK, and phosphorylated (active) PAK (p-PAK) localize at FAs in WT MKs, whereas Fak KO FAs still contain βPIX but show severely reduced staining for PKL, PAK, and p-PAK. Phalloidin (red) marks F-actin, and DAPI (blue) marks chromatin.

    Journal: The Journal of Cell Biology

    Article Title: Focal adhesion kinase modulates tension signaling to control actin and focal adhesion dynamics

    doi: 10.1083/jcb.200608010

    Figure Lengend Snippet: In the absence of FAK, PKL and PAK activity are diminished at FAs. (A) Immunoblot analyses reveal reduced levels of PKL without major differences in levels of PAK or PAK-interacting guanine nucleotide exchange factor (βPIX; actin is control). (B) Immunofluorescence microscopy shows that PKL, βPIX, PAK, and phosphorylated (active) PAK (p-PAK) localize at FAs in WT MKs, whereas Fak KO FAs still contain βPIX but show severely reduced staining for PKL, PAK, and p-PAK. Phalloidin (red) marks F-actin, and DAPI (blue) marks chromatin.

    Article Snippet: Immunofluorescence and immunoprecipitations Tissues or cells were subjected to immunofluorescence microscopy and analyzed using a LSM 510 confocal microscope or a spinning-disc confocal microscope (PerkinElmer). mAbs used were as follows: rat anti-β1 (1:100; Chemicon), CD29 (9EG7; BD Biosciences), α2 (1:50; BD Biosciences), α6 (1:50; BD Biosciences), αvβ6 (1:100; Chemicon), β4 (1:50; BD Biosciences), β-tubulin (1:200; Sigma-Aldrich), mouse anti-VIN (Sigma-Aldrich), FAK (Upstate Biotechnology), PXN (Upstate Biotechnology), ILK (Upstate Biotechnology), Src (Upstate Biotechnology), PKL (BD Bioscience), MLC (Sigma-Aldrich), phosphotyrosine (4G10; Upstate Biotechnology), E-cadherin (Developmental Studies Hybridoma Bank), Erk1/2 (Sigma-Aldrich), and actin (Sigma-Aldrich).

    Techniques: Activity Assay, Immunofluorescence, Microscopy, Staining