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  • 94
    Millipore phenylarsine oxide pao
    Phenylarsine Oxide Pao, supplied by Millipore, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/phenylarsine oxide pao/product/Millipore
    Average 94 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    phenylarsine oxide pao - by Bioz Stars, 2021-05
    94/100 stars
      Buy from Supplier

    86
    Nikken SEIL pao test
    Pao Test, supplied by Nikken SEIL, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/pao test/product/Nikken SEIL
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    pao test - by Bioz Stars, 2021-05
    86/100 stars
      Buy from Supplier

    86
    Bio-Rad pao treatment
    A Western blot shows the effect of <t>PAO</t> on the membrane fraction of <t>ROMK1</t> labeled with biotin The cells transfected with ROMK1 + c-Src + dynaminK44A/vector were treated with PAO for 15 min followed by biotin labeling at 4 °C. The ROMK1 proteins were harvested by immunoprecipitation ( IP ) of cell lysate with GFP antibody. The membrane fraction of ROMK1 was detected with neutravidin ( left panel ), and the total ROMK1 proteins were identified with ROMK antibody ( right panel ). The data were normalized according to the total K + channel protein level. IB , immunoblotting.
    Pao Treatment, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/pao treatment/product/Bio-Rad
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    pao treatment - by Bioz Stars, 2021-05
    86/100 stars
      Buy from Supplier

    N/A
    Gene Silencers generally consist of pools of three to five target specific 19 25 nucleotide sequences in length For independent verification of PAO gene silencing results individual duplex components or
      Buy from Supplier

    N/A
    Gene Silencers generally consist of pools of three to five target specific 19 25 nucleotide sequences in length For independent verification of PAO gene silencing results individual duplex components or
      Buy from Supplier

    N/A
    Gene Silencers generally consist of pools of three to five target specific 19 25 nucleotide sequences in length For independent verification of PAO gene silencing results individual duplex components or
      Buy from Supplier

    N/A
    CRISPR Cas9 KO Plasmids consists of PAO specific 20 nt guide RNA sequences derived from the GeCKO v2 library For CRISPR gene knockout gRNA sequences direct the Cas9 protein to
      Buy from Supplier

    Image Search Results


    A Western blot shows the effect of PAO on the membrane fraction of ROMK1 labeled with biotin The cells transfected with ROMK1 + c-Src + dynaminK44A/vector were treated with PAO for 15 min followed by biotin labeling at 4 °C. The ROMK1 proteins were harvested by immunoprecipitation ( IP ) of cell lysate with GFP antibody. The membrane fraction of ROMK1 was detected with neutravidin ( left panel ), and the total ROMK1 proteins were identified with ROMK antibody ( right panel ). The data were normalized according to the total K + channel protein level. IB , immunoblotting.

    Journal: The Journal of biological chemistry

    Article Title: Inhibition of Protein-tyrosine Phosphatase Stimulates the Dynamin-dependent Endocytosis of ROMK1

    doi: 10.1074/jbc.M109739200

    Figure Lengend Snippet: A Western blot shows the effect of PAO on the membrane fraction of ROMK1 labeled with biotin The cells transfected with ROMK1 + c-Src + dynaminK44A/vector were treated with PAO for 15 min followed by biotin labeling at 4 °C. The ROMK1 proteins were harvested by immunoprecipitation ( IP ) of cell lysate with GFP antibody. The membrane fraction of ROMK1 was detected with neutravidin ( left panel ), and the total ROMK1 proteins were identified with ROMK antibody ( right panel ). The data were normalized according to the total K + channel protein level. IB , immunoblotting.

    Article Snippet: From inspection of , it is apparent that the membrane location of ROMK1 diminished whereas the density of ROMK1 in the intracellular compartment increased significantly within 15 min following PAO treatment.

    Techniques: Western Blot, Labeling, Transfection, Plasmid Preparation, Immunoprecipitation

    A confocal image showing the effect of PAO on the distribution of ROMK1 channels in HEK293 cells transfected with dynaminK44A, GFP-ROMK1, and c-Src The magnification of the picture is ×600, and the length of the bar represents 10 μ m. The cell image under control conditions ( A ) was taken right before addition of PAO. B, C , and D show the channel location following addition of PAO at 5 min ( B ), 10 min ( C ), and 15 min ( D ), respectively.

    Journal: The Journal of biological chemistry

    Article Title: Inhibition of Protein-tyrosine Phosphatase Stimulates the Dynamin-dependent Endocytosis of ROMK1

    doi: 10.1074/jbc.M109739200

    Figure Lengend Snippet: A confocal image showing the effect of PAO on the distribution of ROMK1 channels in HEK293 cells transfected with dynaminK44A, GFP-ROMK1, and c-Src The magnification of the picture is ×600, and the length of the bar represents 10 μ m. The cell image under control conditions ( A ) was taken right before addition of PAO. B, C , and D show the channel location following addition of PAO at 5 min ( B ), 10 min ( C ), and 15 min ( D ), respectively.

    Article Snippet: From inspection of , it is apparent that the membrane location of ROMK1 diminished whereas the density of ROMK1 in the intracellular compartment increased significantly within 15 min following PAO treatment.

    Techniques: Transfection

    A confocal image showing the effect of PAO on the distribution of R1Y337A, a ROMK1 mutant in which tyrosine residue 337 was mutated to alanine The magnification of the picture is ×600, and the length of the bar represents 10 μ m. The cell image under control conditions ( A ) was taken right before addition of PAO. B, C , and D show the channel location following addition of PAO at 5 min ( B ), 10 min ( C ), and 15 min ( D ), respectively.

    Journal: The Journal of biological chemistry

    Article Title: Inhibition of Protein-tyrosine Phosphatase Stimulates the Dynamin-dependent Endocytosis of ROMK1

    doi: 10.1074/jbc.M109739200

    Figure Lengend Snippet: A confocal image showing the effect of PAO on the distribution of R1Y337A, a ROMK1 mutant in which tyrosine residue 337 was mutated to alanine The magnification of the picture is ×600, and the length of the bar represents 10 μ m. The cell image under control conditions ( A ) was taken right before addition of PAO. B, C , and D show the channel location following addition of PAO at 5 min ( B ), 10 min ( C ), and 15 min ( D ), respectively.

    Article Snippet: From inspection of , it is apparent that the membrane location of ROMK1 diminished whereas the density of ROMK1 in the intracellular compartment increased significantly within 15 min following PAO treatment.

    Techniques: Mutagenesis

    A Western blot shows the effect of PAO on the membrane fraction of ROMK1 or R1Y337A labeled by biotin The cells transfected with ROMK1/R1Y337A + c-Src were treated with PAO for 15 min followed by biotin labeling at 4 °C. The ROMK1/R1Y337A proteins were harvested by immunoprecipitation ( IP ) of cell lysate with GFP antibody. The membrane fraction of the K + channels was detected with neutravidin horseradish peroxidase ( left panel ), and the total ROMK1/R1Y337A proteins were identified with ROMK antibody ( right panel ). The data were normalized according to the total K + channel protein level. IB , immunoblotting.

    Journal: The Journal of biological chemistry

    Article Title: Inhibition of Protein-tyrosine Phosphatase Stimulates the Dynamin-dependent Endocytosis of ROMK1

    doi: 10.1074/jbc.M109739200

    Figure Lengend Snippet: A Western blot shows the effect of PAO on the membrane fraction of ROMK1 or R1Y337A labeled by biotin The cells transfected with ROMK1/R1Y337A + c-Src were treated with PAO for 15 min followed by biotin labeling at 4 °C. The ROMK1/R1Y337A proteins were harvested by immunoprecipitation ( IP ) of cell lysate with GFP antibody. The membrane fraction of the K + channels was detected with neutravidin horseradish peroxidase ( left panel ), and the total ROMK1/R1Y337A proteins were identified with ROMK antibody ( right panel ). The data were normalized according to the total K + channel protein level. IB , immunoblotting.

    Article Snippet: From inspection of , it is apparent that the membrane location of ROMK1 diminished whereas the density of ROMK1 in the intracellular compartment increased significantly within 15 min following PAO treatment.

    Techniques: Western Blot, Labeling, Transfection, Immunoprecipitation

    A Western blot illustrating the effect of PAO on the tyrosine phosphorylation level of ROMK1 in the presence and absence of PAO The cells were treated with PAO or vehicle for 15 min. The ROMK1 channels were harvested by immunoprecipitation ( IP ) of the cell lysate with GFP antibody. The phosphorylated ROMK1 was detected with PY20 ( left panel ), and the total ROMK1 is recognized by ROMK antibody ( right panel ). The protein phosphorylation level is normalized by comparison to the relative amount of the total ROMK1 protein. IB , immunoblotting.

    Journal: The Journal of biological chemistry

    Article Title: Inhibition of Protein-tyrosine Phosphatase Stimulates the Dynamin-dependent Endocytosis of ROMK1

    doi: 10.1074/jbc.M109739200

    Figure Lengend Snippet: A Western blot illustrating the effect of PAO on the tyrosine phosphorylation level of ROMK1 in the presence and absence of PAO The cells were treated with PAO or vehicle for 15 min. The ROMK1 channels were harvested by immunoprecipitation ( IP ) of the cell lysate with GFP antibody. The phosphorylated ROMK1 was detected with PY20 ( left panel ), and the total ROMK1 is recognized by ROMK antibody ( right panel ). The protein phosphorylation level is normalized by comparison to the relative amount of the total ROMK1 protein. IB , immunoblotting.

    Article Snippet: From inspection of , it is apparent that the membrane location of ROMK1 diminished whereas the density of ROMK1 in the intracellular compartment increased significantly within 15 min following PAO treatment.

    Techniques: Western Blot, Immunoprecipitation