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  • 91
    Valiant phenylarsine oxide pao
    Phenylarsine Oxide Pao, supplied by Valiant, used in various techniques. Bioz Stars score: 91/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    ATCC pseudomonas aeruginosa pao 1
    Pseudomonas Aeruginosa Pao 1, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 8 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Millipore phenylarsine oxide pao
    A: Analysis of the effects of various treatments on the localization of cortactinY421/actin in the sealing ring of resorbing osteoclasts. Osteoclasts expressing CA-Src protein were treated with PBS, <t>PAO,</t> and alendronate. Confocal microscopy images of osteoclasts stained for cortactin Y421 (green) and actin(red) are shown. Colocalization is observed in yellow color. Scalebar, 25μm. B,C:Measurement of F-actincontent. F-actin contentwas measured by rhodamine <t>phalloidin</t> binding in osteoclasts treated as indicated in the figure. Cells were grown in 24-well tissue culture plates, and 3–4 wells were used for each treatment.The results presented are mean ± SE for three experiments. In part (B), **P
    Phenylarsine Oxide Pao, supplied by Millipore, used in various techniques. Bioz Stars score: 99/100, based on 249 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    85
    Nikken SEIL pao test
    A: Analysis of the effects of various treatments on the localization of cortactinY421/actin in the sealing ring of resorbing osteoclasts. Osteoclasts expressing CA-Src protein were treated with PBS, <t>PAO,</t> and alendronate. Confocal microscopy images of osteoclasts stained for cortactin Y421 (green) and actin(red) are shown. Colocalization is observed in yellow color. Scalebar, 25μm. B,C:Measurement of F-actincontent. F-actin contentwas measured by rhodamine <t>phalloidin</t> binding in osteoclasts treated as indicated in the figure. Cells were grown in 24-well tissue culture plates, and 3–4 wells were used for each treatment.The results presented are mean ± SE for three experiments. In part (B), **P
    Pao Test, supplied by Nikken SEIL, used in various techniques. Bioz Stars score: 85/100, based on 7 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    85
    Bio-Rad pao treatment
    A Western blot shows the effect of <t>PAO</t> on the membrane fraction of <t>ROMK1</t> labeled with biotin The cells transfected with ROMK1 + c-Src + dynaminK44A/vector were treated with PAO for 15 min followed by biotin labeling at 4 °C. The ROMK1 proteins were harvested by immunoprecipitation ( IP ) of cell lysate with GFP antibody. The membrane fraction of ROMK1 was detected with neutravidin ( left panel ), and the total ROMK1 proteins were identified with ROMK antibody ( right panel ). The data were normalized according to the total K + channel protein level. IB , immunoblotting.
    Pao Treatment, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 85/100, based on 10 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    92
    NanoString Technologies Inc custom codeset pao
    NanoString multiplex gene expression analysis of total RNA from whole VNO mucosae. (A) Gene expression profiling of V2R genes by NanoString analysis with the custom <t>CodeSet</t> <t>Pao</t> for strains ∆C1 (red) and ∆C1‐ GFP (green). Six WT and six homozygous ( MUT ) mice were analyzed for each strain. Mice were 10‐week‐old males. The log 2 values of the fold change ( FC ) of MUT over WT are plotted. Filled triangles indicate the Vmn2r1/C1 gene: Its NanoString count in MUT mice is near background level. Filled diamonds indicate differentially expressed ( DE ) genes, with a false discovery rate ( FDR )
    Custom Codeset Pao, supplied by NanoString Technologies Inc, used in various techniques. Bioz Stars score: 92/100, based on 18 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    91
    Santa Cruz Biotechnology pao
    NanoString multiplex gene expression analysis of total RNA from whole VNO mucosae. (A) Gene expression profiling of V2R genes by NanoString analysis with the custom <t>CodeSet</t> <t>Pao</t> for strains ∆C1 (red) and ∆C1‐ GFP (green). Six WT and six homozygous ( MUT ) mice were analyzed for each strain. Mice were 10‐week‐old males. The log 2 values of the fold change ( FC ) of MUT over WT are plotted. Filled triangles indicate the Vmn2r1/C1 gene: Its NanoString count in MUT mice is near background level. Filled diamonds indicate differentially expressed ( DE ) genes, with a false discovery rate ( FDR )
    Pao, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 91/100, based on 6 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    85
    Genmed plant pao assay kit
    NanoString multiplex gene expression analysis of total RNA from whole VNO mucosae. (A) Gene expression profiling of V2R genes by NanoString analysis with the custom <t>CodeSet</t> <t>Pao</t> for strains ∆C1 (red) and ∆C1‐ GFP (green). Six WT and six homozygous ( MUT ) mice were analyzed for each strain. Mice were 10‐week‐old males. The log 2 values of the fold change ( FC ) of MUT over WT are plotted. Filled triangles indicate the Vmn2r1/C1 gene: Its NanoString count in MUT mice is near background level. Filled diamonds indicate differentially expressed ( DE ) genes, with a false discovery rate ( FDR )
    Plant Pao Assay Kit, supplied by Genmed, used in various techniques. Bioz Stars score: 85/100, based on 8 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    93
    Elekta pao alto
    NanoString multiplex gene expression analysis of total RNA from whole VNO mucosae. (A) Gene expression profiling of V2R genes by NanoString analysis with the custom <t>CodeSet</t> <t>Pao</t> for strains ∆C1 (red) and ∆C1‐ GFP (green). Six WT and six homozygous ( MUT ) mice were analyzed for each strain. Mice were 10‐week‐old males. The log 2 values of the fold change ( FC ) of MUT over WT are plotted. Filled triangles indicate the Vmn2r1/C1 gene: Its NanoString count in MUT mice is near background level. Filled diamonds indicate differentially expressed ( DE ) genes, with a false discovery rate ( FDR )
    Pao Alto, supplied by Elekta, used in various techniques. Bioz Stars score: 93/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Agrisera pao antibodies
    . B, Immunoblot analyses of total protein extracts from 3-, 6-, 9-, and 12-DPA developing Col-0 siliques, whose seeds have been removed manually. Total protein extracts obtained from identical silique fresh weight were fractionated by SDS-PAGE (12% polyacrylamide) and transferred on polyvinylidene difluoride filters. Filters were then immunodecorated using antibodies specific for <t>LhcA2,</t> LhcB3, PsbO, PRPS1, PRPL4, <t>PAO,</t> and Histone H3 proteins. A replicate SDS-PAGE gel stained with Coomassie Brilliant Blue (C.B.B.) is shown as a loading control. One out of three immunoblots for each antibody is shown.
    Pao Antibodies, supplied by Agrisera, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Nikken SEIL pao kit
    . B, Immunoblot analyses of total protein extracts from 3-, 6-, 9-, and 12-DPA developing Col-0 siliques, whose seeds have been removed manually. Total protein extracts obtained from identical silique fresh weight were fractionated by SDS-PAGE (12% polyacrylamide) and transferred on polyvinylidene difluoride filters. Filters were then immunodecorated using antibodies specific for <t>LhcA2,</t> LhcB3, PsbO, PRPS1, PRPL4, <t>PAO,</t> and Histone H3 proteins. A replicate SDS-PAGE gel stained with Coomassie Brilliant Blue (C.B.B.) is shown as a loading control. One out of three immunoblots for each antibody is shown.
    Pao Kit, supplied by Nikken SEIL, used in various techniques. Bioz Stars score: 93/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Toronto Research Chemicals 4 amino pao
    . B, Immunoblot analyses of total protein extracts from 3-, 6-, 9-, and 12-DPA developing Col-0 siliques, whose seeds have been removed manually. Total protein extracts obtained from identical silique fresh weight were fractionated by SDS-PAGE (12% polyacrylamide) and transferred on polyvinylidene difluoride filters. Filters were then immunodecorated using antibodies specific for <t>LhcA2,</t> LhcB3, PsbO, PRPS1, PRPL4, <t>PAO,</t> and Histone H3 proteins. A replicate SDS-PAGE gel stained with Coomassie Brilliant Blue (C.B.B.) is shown as a loading control. One out of three immunoblots for each antibody is shown.
    4 Amino Pao, supplied by Toronto Research Chemicals, used in various techniques. Bioz Stars score: 85/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    90
    Mylan advocacy organizations paos
    . B, Immunoblot analyses of total protein extracts from 3-, 6-, 9-, and 12-DPA developing Col-0 siliques, whose seeds have been removed manually. Total protein extracts obtained from identical silique fresh weight were fractionated by SDS-PAGE (12% polyacrylamide) and transferred on polyvinylidene difluoride filters. Filters were then immunodecorated using antibodies specific for <t>LhcA2,</t> LhcB3, PsbO, PRPS1, PRPL4, <t>PAO,</t> and Histone H3 proteins. A replicate SDS-PAGE gel stained with Coomassie Brilliant Blue (C.B.B.) is shown as a loading control. One out of three immunoblots for each antibody is shown.
    Advocacy Organizations Paos, supplied by Mylan, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    99
    Thermo Fisher expression invitrogen pao vector
    . B, Immunoblot analyses of total protein extracts from 3-, 6-, 9-, and 12-DPA developing Col-0 siliques, whose seeds have been removed manually. Total protein extracts obtained from identical silique fresh weight were fractionated by SDS-PAGE (12% polyacrylamide) and transferred on polyvinylidene difluoride filters. Filters were then immunodecorated using antibodies specific for <t>LhcA2,</t> LhcB3, PsbO, PRPS1, PRPL4, <t>PAO,</t> and Histone H3 proteins. A replicate SDS-PAGE gel stained with Coomassie Brilliant Blue (C.B.B.) is shown as a loading control. One out of three immunoblots for each antibody is shown.
    Expression Invitrogen Pao Vector, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Millipore palmitoleic acid pao
    . B, Immunoblot analyses of total protein extracts from 3-, 6-, 9-, and 12-DPA developing Col-0 siliques, whose seeds have been removed manually. Total protein extracts obtained from identical silique fresh weight were fractionated by SDS-PAGE (12% polyacrylamide) and transferred on polyvinylidene difluoride filters. Filters were then immunodecorated using antibodies specific for <t>LhcA2,</t> LhcB3, PsbO, PRPS1, PRPL4, <t>PAO,</t> and Histone H3 proteins. A replicate SDS-PAGE gel stained with Coomassie Brilliant Blue (C.B.B.) is shown as a loading control. One out of three immunoblots for each antibody is shown.
    Palmitoleic Acid Pao, supplied by Millipore, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Millipore maize pao rabbit polyclonal antibody
    . B, Immunoblot analyses of total protein extracts from 3-, 6-, 9-, and 12-DPA developing Col-0 siliques, whose seeds have been removed manually. Total protein extracts obtained from identical silique fresh weight were fractionated by SDS-PAGE (12% polyacrylamide) and transferred on polyvinylidene difluoride filters. Filters were then immunodecorated using antibodies specific for <t>LhcA2,</t> LhcB3, PsbO, PRPS1, PRPL4, <t>PAO,</t> and Histone H3 proteins. A replicate SDS-PAGE gel stained with Coomassie Brilliant Blue (C.B.B.) is shown as a loading control. One out of three immunoblots for each antibody is shown.
    Maize Pao Rabbit Polyclonal Antibody, supplied by Millipore, used in various techniques. Bioz Stars score: 99/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Image Search Results


    A: Analysis of the effects of various treatments on the localization of cortactinY421/actin in the sealing ring of resorbing osteoclasts. Osteoclasts expressing CA-Src protein were treated with PBS, PAO, and alendronate. Confocal microscopy images of osteoclasts stained for cortactin Y421 (green) and actin(red) are shown. Colocalization is observed in yellow color. Scalebar, 25μm. B,C:Measurement of F-actincontent. F-actin contentwas measured by rhodamine phalloidin binding in osteoclasts treated as indicated in the figure. Cells were grown in 24-well tissue culture plates, and 3–4 wells were used for each treatment.The results presented are mean ± SE for three experiments. In part (B), **P

    Journal: Journal of cellular physiology

    Article Title: Activation of Src Kinase by Protein–Tyrosine Phosphatase–PEST in Osteoclasts: Comparative Analysis of the Effects of Bisphosphonate and Protein–Tyrosine Phosphatase Inhibitor on Src Activation In Vitro

    doi: 10.1002/jcp.21777

    Figure Lengend Snippet: A: Analysis of the effects of various treatments on the localization of cortactinY421/actin in the sealing ring of resorbing osteoclasts. Osteoclasts expressing CA-Src protein were treated with PBS, PAO, and alendronate. Confocal microscopy images of osteoclasts stained for cortactin Y421 (green) and actin(red) are shown. Colocalization is observed in yellow color. Scalebar, 25μm. B,C:Measurement of F-actincontent. F-actin contentwas measured by rhodamine phalloidin binding in osteoclasts treated as indicated in the figure. Cells were grown in 24-well tissue culture plates, and 3–4 wells were used for each treatment.The results presented are mean ± SE for three experiments. In part (B), **P

    Article Snippet: Rhodamine phalloidin, phenyl arsine oxide (PAO), a rabbit polyclonal antibody against phosphocortactin Y421, and all the other chemicals were purchased from Sigma–Aldrich Chemical Co. (St. Louis, MO).

    Techniques: Expressing, Confocal Microscopy, Staining, Binding Assay

    Effects of membrane trafficking inhibitors on stomatal responses to bicarbonate, darkness, and ABA. Epidermal strips were placed in opening buffer for 1 h in the light before being transferred to darkness or opening buffer containing 2 mM bicarbonate or 10 μM ABA with/without inhibitors (2.5 μM PAO, 70 μM LY294002, 70 μM LY83583, 70 μM W-7) for 2 h. Error bars indicate ±SE, and lowercase letters represent significantly different groups ( n ≥ 120 stomata per each treatment from four or five independent experiments; P

    Journal: Frontiers in Plant Science

    Article Title: Differential Effects of Phosphatidylinositol 4-Kinase (PI4K) and 3-Kinase (PI3K) Inhibitors on Stomatal Responses to Environmental Signals

    doi: 10.3389/fpls.2017.00677

    Figure Lengend Snippet: Effects of membrane trafficking inhibitors on stomatal responses to bicarbonate, darkness, and ABA. Epidermal strips were placed in opening buffer for 1 h in the light before being transferred to darkness or opening buffer containing 2 mM bicarbonate or 10 μM ABA with/without inhibitors (2.5 μM PAO, 70 μM LY294002, 70 μM LY83583, 70 μM W-7) for 2 h. Error bars indicate ±SE, and lowercase letters represent significantly different groups ( n ≥ 120 stomata per each treatment from four or five independent experiments; P

    Article Snippet: Chemicals PAO (Sigma), LY294002 (2-morpholin-4-yl-8-phenylchromen-4-one) (Tokyo Chemical Industry), LY83583 [6-(phenylamino)-5,8-dihydroquinoline-5,8-dione] (Cayman Chemical Company), brefeldin A ((1R ,2E ,6S ,10E ,11aS ,13S ,14aR )-1,13-dihydroxy-6-methyl-1,6,7,8,9,11a,12,13,14,14a-decahydro-4H -cyclopenta [f ]oxacyclotridecin-4-one) (Wako Pure Chemical Industries), W-7 [N -(6-aminohexyl)-5-chloro-1-naphthalenesulfonamide hydrochloride] (Wako Pure Chemical Industries), N -ethylmaleimide (1-ethylpyrrole-2,5-dione) (Tokyo Chemical Industry), and propyzamide (Wako Pure Chemical Industries) stock solutions (100 mM) were prepared in 100% DMSO.

    Techniques:

    Effects of PAO and LY294002 on GFP-PATROL1 dot densities in cotyledonary guard cells in response to bicarbonate, darkness, and ABA. (A) Cotyledons were placed in opening buffer for 1 h in white light before being transferred to darkness or opening buffer containing 2 mM bicarbonate or 10 μM ABA with/without 2.5 μM PAO for 2 h. Error bars indicate ±SE of 12 independent experiments, and lowercase letters represent significantly different groups ( P

    Journal: Frontiers in Plant Science

    Article Title: Differential Effects of Phosphatidylinositol 4-Kinase (PI4K) and 3-Kinase (PI3K) Inhibitors on Stomatal Responses to Environmental Signals

    doi: 10.3389/fpls.2017.00677

    Figure Lengend Snippet: Effects of PAO and LY294002 on GFP-PATROL1 dot densities in cotyledonary guard cells in response to bicarbonate, darkness, and ABA. (A) Cotyledons were placed in opening buffer for 1 h in white light before being transferred to darkness or opening buffer containing 2 mM bicarbonate or 10 μM ABA with/without 2.5 μM PAO for 2 h. Error bars indicate ±SE of 12 independent experiments, and lowercase letters represent significantly different groups ( P

    Article Snippet: Chemicals PAO (Sigma), LY294002 (2-morpholin-4-yl-8-phenylchromen-4-one) (Tokyo Chemical Industry), LY83583 [6-(phenylamino)-5,8-dihydroquinoline-5,8-dione] (Cayman Chemical Company), brefeldin A ((1R ,2E ,6S ,10E ,11aS ,13S ,14aR )-1,13-dihydroxy-6-methyl-1,6,7,8,9,11a,12,13,14,14a-decahydro-4H -cyclopenta [f ]oxacyclotridecin-4-one) (Wako Pure Chemical Industries), W-7 [N -(6-aminohexyl)-5-chloro-1-naphthalenesulfonamide hydrochloride] (Wako Pure Chemical Industries), N -ethylmaleimide (1-ethylpyrrole-2,5-dione) (Tokyo Chemical Industry), and propyzamide (Wako Pure Chemical Industries) stock solutions (100 mM) were prepared in 100% DMSO.

    Techniques:

    A Western blot shows the effect of PAO on the membrane fraction of ROMK1 labeled with biotin The cells transfected with ROMK1 + c-Src + dynaminK44A/vector were treated with PAO for 15 min followed by biotin labeling at 4 °C. The ROMK1 proteins were harvested by immunoprecipitation ( IP ) of cell lysate with GFP antibody. The membrane fraction of ROMK1 was detected with neutravidin ( left panel ), and the total ROMK1 proteins were identified with ROMK antibody ( right panel ). The data were normalized according to the total K + channel protein level. IB , immunoblotting.

    Journal: The Journal of biological chemistry

    Article Title: Inhibition of Protein-tyrosine Phosphatase Stimulates the Dynamin-dependent Endocytosis of ROMK1

    doi: 10.1074/jbc.M109739200

    Figure Lengend Snippet: A Western blot shows the effect of PAO on the membrane fraction of ROMK1 labeled with biotin The cells transfected with ROMK1 + c-Src + dynaminK44A/vector were treated with PAO for 15 min followed by biotin labeling at 4 °C. The ROMK1 proteins were harvested by immunoprecipitation ( IP ) of cell lysate with GFP antibody. The membrane fraction of ROMK1 was detected with neutravidin ( left panel ), and the total ROMK1 proteins were identified with ROMK antibody ( right panel ). The data were normalized according to the total K + channel protein level. IB , immunoblotting.

    Article Snippet: First, our present study shows that PAO treatment increased the tyrosine phosphorylation of ROMK1.

    Techniques: Western Blot, Labeling, Transfection, Plasmid Preparation, Immunoprecipitation

    A confocal image showing the effect of PAO on the distribution of ROMK1 channels in HEK293 cells transfected with dynaminK44A, GFP-ROMK1, and c-Src The magnification of the picture is ×600, and the length of the bar represents 10 μ m. The cell image under control conditions ( A ) was taken right before addition of PAO. B, C , and D show the channel location following addition of PAO at 5 min ( B ), 10 min ( C ), and 15 min ( D ), respectively.

    Journal: The Journal of biological chemistry

    Article Title: Inhibition of Protein-tyrosine Phosphatase Stimulates the Dynamin-dependent Endocytosis of ROMK1

    doi: 10.1074/jbc.M109739200

    Figure Lengend Snippet: A confocal image showing the effect of PAO on the distribution of ROMK1 channels in HEK293 cells transfected with dynaminK44A, GFP-ROMK1, and c-Src The magnification of the picture is ×600, and the length of the bar represents 10 μ m. The cell image under control conditions ( A ) was taken right before addition of PAO. B, C , and D show the channel location following addition of PAO at 5 min ( B ), 10 min ( C ), and 15 min ( D ), respectively.

    Article Snippet: First, our present study shows that PAO treatment increased the tyrosine phosphorylation of ROMK1.

    Techniques: Transfection

    A confocal image showing the effect of PAO on the distribution of R1Y337A, a ROMK1 mutant in which tyrosine residue 337 was mutated to alanine The magnification of the picture is ×600, and the length of the bar represents 10 μ m. The cell image under control conditions ( A ) was taken right before addition of PAO. B, C , and D show the channel location following addition of PAO at 5 min ( B ), 10 min ( C ), and 15 min ( D ), respectively.

    Journal: The Journal of biological chemistry

    Article Title: Inhibition of Protein-tyrosine Phosphatase Stimulates the Dynamin-dependent Endocytosis of ROMK1

    doi: 10.1074/jbc.M109739200

    Figure Lengend Snippet: A confocal image showing the effect of PAO on the distribution of R1Y337A, a ROMK1 mutant in which tyrosine residue 337 was mutated to alanine The magnification of the picture is ×600, and the length of the bar represents 10 μ m. The cell image under control conditions ( A ) was taken right before addition of PAO. B, C , and D show the channel location following addition of PAO at 5 min ( B ), 10 min ( C ), and 15 min ( D ), respectively.

    Article Snippet: First, our present study shows that PAO treatment increased the tyrosine phosphorylation of ROMK1.

    Techniques: Mutagenesis

    A Western blot shows the effect of PAO on the membrane fraction of ROMK1 or R1Y337A labeled by biotin The cells transfected with ROMK1/R1Y337A + c-Src were treated with PAO for 15 min followed by biotin labeling at 4 °C. The ROMK1/R1Y337A proteins were harvested by immunoprecipitation ( IP ) of cell lysate with GFP antibody. The membrane fraction of the K + channels was detected with neutravidin horseradish peroxidase ( left panel ), and the total ROMK1/R1Y337A proteins were identified with ROMK antibody ( right panel ). The data were normalized according to the total K + channel protein level. IB , immunoblotting.

    Journal: The Journal of biological chemistry

    Article Title: Inhibition of Protein-tyrosine Phosphatase Stimulates the Dynamin-dependent Endocytosis of ROMK1

    doi: 10.1074/jbc.M109739200

    Figure Lengend Snippet: A Western blot shows the effect of PAO on the membrane fraction of ROMK1 or R1Y337A labeled by biotin The cells transfected with ROMK1/R1Y337A + c-Src were treated with PAO for 15 min followed by biotin labeling at 4 °C. The ROMK1/R1Y337A proteins were harvested by immunoprecipitation ( IP ) of cell lysate with GFP antibody. The membrane fraction of the K + channels was detected with neutravidin horseradish peroxidase ( left panel ), and the total ROMK1/R1Y337A proteins were identified with ROMK antibody ( right panel ). The data were normalized according to the total K + channel protein level. IB , immunoblotting.

    Article Snippet: First, our present study shows that PAO treatment increased the tyrosine phosphorylation of ROMK1.

    Techniques: Western Blot, Labeling, Transfection, Immunoprecipitation

    A Western blot illustrating the effect of PAO on the tyrosine phosphorylation level of ROMK1 in the presence and absence of PAO The cells were treated with PAO or vehicle for 15 min. The ROMK1 channels were harvested by immunoprecipitation ( IP ) of the cell lysate with GFP antibody. The phosphorylated ROMK1 was detected with PY20 ( left panel ), and the total ROMK1 is recognized by ROMK antibody ( right panel ). The protein phosphorylation level is normalized by comparison to the relative amount of the total ROMK1 protein. IB , immunoblotting.

    Journal: The Journal of biological chemistry

    Article Title: Inhibition of Protein-tyrosine Phosphatase Stimulates the Dynamin-dependent Endocytosis of ROMK1

    doi: 10.1074/jbc.M109739200

    Figure Lengend Snippet: A Western blot illustrating the effect of PAO on the tyrosine phosphorylation level of ROMK1 in the presence and absence of PAO The cells were treated with PAO or vehicle for 15 min. The ROMK1 channels were harvested by immunoprecipitation ( IP ) of the cell lysate with GFP antibody. The phosphorylated ROMK1 was detected with PY20 ( left panel ), and the total ROMK1 is recognized by ROMK antibody ( right panel ). The protein phosphorylation level is normalized by comparison to the relative amount of the total ROMK1 protein. IB , immunoblotting.

    Article Snippet: First, our present study shows that PAO treatment increased the tyrosine phosphorylation of ROMK1.

    Techniques: Western Blot, Immunoprecipitation

    NanoString multiplex gene expression analysis of total RNA from whole VNO mucosae. (A) Gene expression profiling of V2R genes by NanoString analysis with the custom CodeSet Pao for strains ∆C1 (red) and ∆C1‐ GFP (green). Six WT and six homozygous ( MUT ) mice were analyzed for each strain. Mice were 10‐week‐old males. The log 2 values of the fold change ( FC ) of MUT over WT are plotted. Filled triangles indicate the Vmn2r1/C1 gene: Its NanoString count in MUT mice is near background level. Filled diamonds indicate differentially expressed ( DE ) genes, with a false discovery rate ( FDR )

    Journal: The European Journal of Neuroscience

    Article Title: Subpopulations of vomeronasal sensory neurons with coordinated coexpression of type 2 vomeronasal receptor genes are differentially dependent on Vmn2r1

    doi: 10.1111/ejn.13875

    Figure Lengend Snippet: NanoString multiplex gene expression analysis of total RNA from whole VNO mucosae. (A) Gene expression profiling of V2R genes by NanoString analysis with the custom CodeSet Pao for strains ∆C1 (red) and ∆C1‐ GFP (green). Six WT and six homozygous ( MUT ) mice were analyzed for each strain. Mice were 10‐week‐old males. The log 2 values of the fold change ( FC ) of MUT over WT are plotted. Filled triangles indicate the Vmn2r1/C1 gene: Its NanoString count in MUT mice is near background level. Filled diamonds indicate differentially expressed ( DE ) genes, with a false discovery rate ( FDR )

    Article Snippet: A 0.75 μg aliquot of RNA from each mouse was tested with the custom CodeSet Pao (NanoString Technologies, Seattle, WA, USA).

    Techniques: Multiplex Assay, Expressing, Mouse Assay

    . B, Immunoblot analyses of total protein extracts from 3-, 6-, 9-, and 12-DPA developing Col-0 siliques, whose seeds have been removed manually. Total protein extracts obtained from identical silique fresh weight were fractionated by SDS-PAGE (12% polyacrylamide) and transferred on polyvinylidene difluoride filters. Filters were then immunodecorated using antibodies specific for LhcA2, LhcB3, PsbO, PRPS1, PRPL4, PAO, and Histone H3 proteins. A replicate SDS-PAGE gel stained with Coomassie Brilliant Blue (C.B.B.) is shown as a loading control. One out of three immunoblots for each antibody is shown.

    Journal: Plant Physiology

    Article Title: Time-Course Transcriptome Analysis of Arabidopsis Siliques Discloses Genes Essential for Fruit Development and Maturation

    doi: 10.1104/pp.18.00727

    Figure Lengend Snippet: . B, Immunoblot analyses of total protein extracts from 3-, 6-, 9-, and 12-DPA developing Col-0 siliques, whose seeds have been removed manually. Total protein extracts obtained from identical silique fresh weight were fractionated by SDS-PAGE (12% polyacrylamide) and transferred on polyvinylidene difluoride filters. Filters were then immunodecorated using antibodies specific for LhcA2, LhcB3, PsbO, PRPS1, PRPL4, PAO, and Histone H3 proteins. A replicate SDS-PAGE gel stained with Coomassie Brilliant Blue (C.B.B.) is shown as a loading control. One out of three immunoblots for each antibody is shown.

    Article Snippet: LhcA2, LhcB3, PsbO, and PAO antibodies were obtained from Agrisera.

    Techniques: SDS Page, Staining, Western Blot