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  • 86
    New England Biolabs fetuin
    Optimization of the LDA method using <t>RNase</t> B and <t>fetuin.</t> Experimental conditions for optimal limited deglycosylation of bovine RNase B and fetuin by PNGase F were determined. The optimal A ) NP-40 concentration, B ) temperature, C ) and D ) PNGase F:glycoprotein ratio for RNase B and fetuin, respectively, and, E ) and F ) the optimal time for the deglycosylation of RNase B and fetuin, respectively, were assayed by differential migration of the glycoproteins in 15% SDS-PAGE. Fully deglycosylated (+) and fully glycosylated (−) proteins were included for each optimization reaction, denoted by FD and FG, respectively.
    Fetuin, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/fetuin/product/New England Biolabs
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    fetuin - by Bioz Stars, 2021-06
    86/100 stars
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    95
    New England Biolabs fetuin solution
    Lectin blots of untreated (−), S. typhimurium α2-3 sialidase treated (α2-3 N) or <t>PNGase</t> F (PNG-F) treated bovine <t>fetuin.</t> CBB, Coomassie brilliant blue stain; MAL, Maackia amurensis leukoagglutinin; MAH, Maackia amurensis hemagglutinin;
    Fetuin Solution, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/fetuin solution/product/New England Biolabs
    Average 95 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    fetuin solution - by Bioz Stars, 2021-06
    95/100 stars
      Buy from Supplier

    N/A
    Standard format Plasmid sent in bacteria as agar stab
      Buy from Supplier

    Image Search Results


    Optimization of the LDA method using RNase B and fetuin. Experimental conditions for optimal limited deglycosylation of bovine RNase B and fetuin by PNGase F were determined. The optimal A ) NP-40 concentration, B ) temperature, C ) and D ) PNGase F:glycoprotein ratio for RNase B and fetuin, respectively, and, E ) and F ) the optimal time for the deglycosylation of RNase B and fetuin, respectively, were assayed by differential migration of the glycoproteins in 15% SDS-PAGE. Fully deglycosylated (+) and fully glycosylated (−) proteins were included for each optimization reaction, denoted by FD and FG, respectively.

    Journal: bioRxiv

    Article Title: Systems-wide analysis of glycoprotein conformational changes by limited deglycosylation assay

    doi: 10.1101/2021.06.04.447131

    Figure Lengend Snippet: Optimization of the LDA method using RNase B and fetuin. Experimental conditions for optimal limited deglycosylation of bovine RNase B and fetuin by PNGase F were determined. The optimal A ) NP-40 concentration, B ) temperature, C ) and D ) PNGase F:glycoprotein ratio for RNase B and fetuin, respectively, and, E ) and F ) the optimal time for the deglycosylation of RNase B and fetuin, respectively, were assayed by differential migration of the glycoproteins in 15% SDS-PAGE. Fully deglycosylated (+) and fully glycosylated (−) proteins were included for each optimization reaction, denoted by FD and FG, respectively.

    Article Snippet: The optimal PNGase F (Cat. P0709S, NEB) concentration (relative to the glycoprotein substrate) was tested ranging from 1000, 500, 250, 125 to 75 U/ 5 μg RNase B (Cat.P7817S, NEB) and fetuin (Cat. P6042S, NEB).

    Techniques: Concentration Assay, Migration, SDS Page

    Lectin blots of untreated (−), S. typhimurium α2-3 sialidase treated (α2-3 N) or PNGase F (PNG-F) treated bovine fetuin. CBB, Coomassie brilliant blue stain; MAL, Maackia amurensis leukoagglutinin; MAH, Maackia amurensis hemagglutinin;

    Journal: Glycobiology

    Article Title: Letter to the Glyco-Forum: Effective glycoanalysis with Maackia amurensis lectins requires a clear understanding of their binding specificities

    doi: 10.1093/glycob/cwr080

    Figure Lengend Snippet: Lectin blots of untreated (−), S. typhimurium α2-3 sialidase treated (α2-3 N) or PNGase F (PNG-F) treated bovine fetuin. CBB, Coomassie brilliant blue stain; MAL, Maackia amurensis leukoagglutinin; MAH, Maackia amurensis hemagglutinin;

    Article Snippet: For de- N -glycosylation, samples of the fetuin solution were treated with Flavobacterium meningosepticum PNGase F (New England Biolabs) according to the manufacturer's instructions.

    Techniques: Staining