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  • 93
    New England Biolabs a1 3 4 fucosidase
    The fucose salvage pathway inhibits invadopodia through α1–2 fucosylation. A, schematic illustration showing the modification of glycans by α-1,2, α-1,3 and α-1,4 branched fucosylations. B, the effects of L-fucose and <t>fucosidase</t> treatment on cell surface α1–2 fucosylation, as determined by UEA-1 staining and flow cytometry. C, quantification of the effects of α-1,2 fucosidase treatment on fucose-mediated inhibition of invadopodium formation in WM793 cells. D, representative invadopodium assay images showing the effect of α-1,2 fucosidase treatment on FUK-mediated inhibition of invadopodium formation in WM793 cells. E and F, quantitation of the effect of α-1,2 fucosidase treatment on the invadopodium number per cell ( E ) and the proportion of invadopodia positive cells ( F ) in control or FUK OE WM793. G and H, quantitation of the effect of <t>α-1,3/4</t> fucosidase treatment on the average invadopodium number per cell ( G ) and proportion of invadopodia positive cells ( H ) in control or fucose-treated WM793. I and J, quantitation of the effect of α-1,3/4 fucosidase treatment on the average invadopodium number per cell ( I ) and proportion of invadopodia positive cells ( J ) in control or FUK-overexpressed WM793. ** and *** indicates p
    A1 3 4 Fucosidase, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/a1 3 4 fucosidase/product/New England Biolabs
    Average 93 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    a1 3 4 fucosidase - by Bioz Stars, 2021-06
    93/100 stars
      Buy from Supplier

    92
    Millipore trisodium salt
    The fucose salvage pathway inhibits invadopodia through α1–2 fucosylation. A, schematic illustration showing the modification of glycans by α-1,2, α-1,3 and α-1,4 branched fucosylations. B, the effects of L-fucose and <t>fucosidase</t> treatment on cell surface α1–2 fucosylation, as determined by UEA-1 staining and flow cytometry. C, quantification of the effects of α-1,2 fucosidase treatment on fucose-mediated inhibition of invadopodium formation in WM793 cells. D, representative invadopodium assay images showing the effect of α-1,2 fucosidase treatment on FUK-mediated inhibition of invadopodium formation in WM793 cells. E and F, quantitation of the effect of α-1,2 fucosidase treatment on the invadopodium number per cell ( E ) and the proportion of invadopodia positive cells ( F ) in control or FUK OE WM793. G and H, quantitation of the effect of <t>α-1,3/4</t> fucosidase treatment on the average invadopodium number per cell ( G ) and proportion of invadopodia positive cells ( H ) in control or fucose-treated WM793. I and J, quantitation of the effect of α-1,3/4 fucosidase treatment on the average invadopodium number per cell ( I ) and proportion of invadopodia positive cells ( J ) in control or FUK-overexpressed WM793. ** and *** indicates p
    Trisodium Salt, supplied by Millipore, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/trisodium salt/product/Millipore
    Average 92 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    trisodium salt - by Bioz Stars, 2021-06
    92/100 stars
      Buy from Supplier

    Image Search Results


    The fucose salvage pathway inhibits invadopodia through α1–2 fucosylation. A, schematic illustration showing the modification of glycans by α-1,2, α-1,3 and α-1,4 branched fucosylations. B, the effects of L-fucose and fucosidase treatment on cell surface α1–2 fucosylation, as determined by UEA-1 staining and flow cytometry. C, quantification of the effects of α-1,2 fucosidase treatment on fucose-mediated inhibition of invadopodium formation in WM793 cells. D, representative invadopodium assay images showing the effect of α-1,2 fucosidase treatment on FUK-mediated inhibition of invadopodium formation in WM793 cells. E and F, quantitation of the effect of α-1,2 fucosidase treatment on the invadopodium number per cell ( E ) and the proportion of invadopodia positive cells ( F ) in control or FUK OE WM793. G and H, quantitation of the effect of α-1,3/4 fucosidase treatment on the average invadopodium number per cell ( G ) and proportion of invadopodia positive cells ( H ) in control or fucose-treated WM793. I and J, quantitation of the effect of α-1,3/4 fucosidase treatment on the average invadopodium number per cell ( I ) and proportion of invadopodia positive cells ( J ) in control or FUK-overexpressed WM793. ** and *** indicates p

    Journal: PLoS ONE

    Article Title: The fucose salvage pathway inhibits invadopodia formation and extracellular matrix degradation in melanoma cells

    doi: 10.1371/journal.pone.0199128

    Figure Lengend Snippet: The fucose salvage pathway inhibits invadopodia through α1–2 fucosylation. A, schematic illustration showing the modification of glycans by α-1,2, α-1,3 and α-1,4 branched fucosylations. B, the effects of L-fucose and fucosidase treatment on cell surface α1–2 fucosylation, as determined by UEA-1 staining and flow cytometry. C, quantification of the effects of α-1,2 fucosidase treatment on fucose-mediated inhibition of invadopodium formation in WM793 cells. D, representative invadopodium assay images showing the effect of α-1,2 fucosidase treatment on FUK-mediated inhibition of invadopodium formation in WM793 cells. E and F, quantitation of the effect of α-1,2 fucosidase treatment on the invadopodium number per cell ( E ) and the proportion of invadopodia positive cells ( F ) in control or FUK OE WM793. G and H, quantitation of the effect of α-1,3/4 fucosidase treatment on the average invadopodium number per cell ( G ) and proportion of invadopodia positive cells ( H ) in control or fucose-treated WM793. I and J, quantitation of the effect of α-1,3/4 fucosidase treatment on the average invadopodium number per cell ( I ) and proportion of invadopodia positive cells ( J ) in control or FUK-overexpressed WM793. ** and *** indicates p

    Article Snippet: Fucosidase treatment After cell dissociation using enzyme free solution, cells were washed three times in 1xPBS, then incubated in PBS with 0.08U/mL fucosidase (New England Biolabs, α-1,2:P0724S, α-1,3/4:P0769S) for 30 minutes at 37°C.

    Techniques: Modification, Staining, Flow Cytometry, Cytometry, Inhibition, Quantitation Assay