Journal: PLoS ONE
Article Title: C-Terminal Amino Acids 471-507 of Avian Hepatitis E Virus Capsid Protein Are Crucial for Binding to Avian and Human Cells
Figure Lengend Snippet: Effect of heparin sodium salt, sodium chlorate and heparinase II treatment in binding assay. (a) Western blotting analysis following treatment with heparin sodium salt. Relative intensity of immunofluorescence signal derived from different binding assays (b) heparin sodium salt, (c) sodium chlorate and (d) heparinase II. Mean fluorescence intensity of stained cells was measured with ImageJ. Error bars represent standard deviation. *, p
Article Snippet: Heparinase II treatment of LMH cells LMH cells were pre-treated in Heparinase Reaction Buffer (20 mM Tris-HCl, 100 mM NaCl and 1.5 mM CaCl2 ) with heparinase II (10U/ml) from Bacteroides (New England Biolabs GmbH, Frankfurt am Main, Germany) or PBS as control for 2h at 37°C in an atmosphere supplied with 5% CO2 .
Techniques: Binding Assay, Western Blot, Immunofluorescence, Derivative Assay, Fluorescence, Staining, Standard Deviation