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  • 93
    New England Biolabs heparinase ii
    Effect of heparin sodium salt, sodium chlorate and <t>heparinase</t> II treatment in binding assay. (a) Western blotting analysis following treatment with heparin sodium salt. Relative intensity of immunofluorescence signal derived from different binding assays (b) heparin sodium salt, (c) sodium chlorate and (d) heparinase II. Mean fluorescence intensity of stained cells was measured with ImageJ. Error bars represent standard deviation. *, p
    Heparinase Ii, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 93/100, based on 15 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/heparinase ii/product/New England Biolabs
    Average 93 stars, based on 15 article reviews
    Price from $9.99 to $1999.99
    heparinase ii - by Bioz Stars, 2020-05
    93/100 stars
      Buy from Supplier

    Image Search Results


    Effect of heparin sodium salt, sodium chlorate and heparinase II treatment in binding assay. (a) Western blotting analysis following treatment with heparin sodium salt. Relative intensity of immunofluorescence signal derived from different binding assays (b) heparin sodium salt, (c) sodium chlorate and (d) heparinase II. Mean fluorescence intensity of stained cells was measured with ImageJ. Error bars represent standard deviation. *, p

    Journal: PLoS ONE

    Article Title: C-Terminal Amino Acids 471-507 of Avian Hepatitis E Virus Capsid Protein Are Crucial for Binding to Avian and Human Cells

    doi: 10.1371/journal.pone.0153723

    Figure Lengend Snippet: Effect of heparin sodium salt, sodium chlorate and heparinase II treatment in binding assay. (a) Western blotting analysis following treatment with heparin sodium salt. Relative intensity of immunofluorescence signal derived from different binding assays (b) heparin sodium salt, (c) sodium chlorate and (d) heparinase II. Mean fluorescence intensity of stained cells was measured with ImageJ. Error bars represent standard deviation. *, p

    Article Snippet: Heparinase II treatment of LMH cells LMH cells were pre-treated in Heparinase Reaction Buffer (20 mM Tris-HCl, 100 mM NaCl and 1.5 mM CaCl2 ) with heparinase II (10U/ml) from Bacteroides (New England Biolabs GmbH, Frankfurt am Main, Germany) or PBS as control for 2h at 37°C in an atmosphere supplied with 5% CO2 .

    Techniques: Binding Assay, Western Blot, Immunofluorescence, Derivative Assay, Fluorescence, Staining, Standard Deviation

    LDL binding to the vessel wall in vitro following treatment with Site B peptide or enzymatic digestion of proteoglycan GAG chains. Tissue sections of carotid arteries from wild‐type mice with intimal hyperplasia were incubated with human LDL . Bound LDL was detected using anti‐apoB antibody. (A) LDL binding to tissue sections pre‐incubated with positively charged SiteB peptide (white circles) or neutrally charged SiteB KE peptide (gray circles). Black circles=no pre‐treatment. White triangles=no LDL incubation (buffer only). (B) LDL binding to tissue sections pre‐treated with the GAG ‐degrading enzymes chondroitinase (white circles) or heparinase (white dot circles). Black circles=no pre‐treatment. White triangles=no LDL incubation (buffer only). Data was analyzed using Mann–Whitney rank sum test. Graph bars show median values. P

    Journal: Physiological Reports

    Article Title: Intimal hyperplasia induced by vascular intervention causes lipoprotein retention and accelerated atherosclerosis. Intimal hyperplasia induced by vascular intervention causes lipoprotein retention and accelerated atherosclerosis

    doi: 10.14814/phy2.13334

    Figure Lengend Snippet: LDL binding to the vessel wall in vitro following treatment with Site B peptide or enzymatic digestion of proteoglycan GAG chains. Tissue sections of carotid arteries from wild‐type mice with intimal hyperplasia were incubated with human LDL . Bound LDL was detected using anti‐apoB antibody. (A) LDL binding to tissue sections pre‐incubated with positively charged SiteB peptide (white circles) or neutrally charged SiteB KE peptide (gray circles). Black circles=no pre‐treatment. White triangles=no LDL incubation (buffer only). (B) LDL binding to tissue sections pre‐treated with the GAG ‐degrading enzymes chondroitinase (white circles) or heparinase (white dot circles). Black circles=no pre‐treatment. White triangles=no LDL incubation (buffer only). Data was analyzed using Mann–Whitney rank sum test. Graph bars show median values. P

    Article Snippet: For GAG enzyme experiments, tissue sections were pre‐incubated 30 min at RT with Heparinase II (New England BioLabs, P0736S) or Chondrotinase ABC (Sigma, C3667) as per manufacturer's recommendations.

    Techniques: Binding Assay, In Vitro, Mouse Assay, Incubation, MANN-WHITNEY