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  • 97
    Abcam goat anti rabbit igg h l hrp
    Goat Anti Rabbit Igg H L Hrp, supplied by Abcam, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Abcam goat anti mouse igg h l hrp
    Goat Anti Mouse Igg H L Hrp, supplied by Abcam, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    93
    Boster Bio phosphospecific rabbit anti akt ser473 antibody
    ( A and D ) Western blot analysis for the inhibitory effects of OXY on the phosphorylation of AKT, p70S6K, and ERK1/2 in cells treated with various concentrations of OXY (10, 20, and 40 μM) for 4 h before stimulation with IL-1β for 20 min; ( B ) quantitative analysis for AKT phosphorylation at <t>Ser473;</t> ( C ) quantitative analysis for p70S6K phosphorylation; ( E ) quantitative analysis for ERK1/2 phosphorylation. Total AKT, total ERK1/2, and beta actin (β-actin) were detected and used as an internal control. Data are represented as mean ± SD of three independent experiments; # p
    Phosphospecific Rabbit Anti Akt Ser473 Antibody, supplied by Boster Bio, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    86
    Alpha Innotech alphaease fc software
    ( A and D ) Western blot analysis for the inhibitory effects of OXY on the phosphorylation of AKT, p70S6K, and ERK1/2 in cells treated with various concentrations of OXY (10, 20, and 40 μM) for 4 h before stimulation with IL-1β for 20 min; ( B ) quantitative analysis for AKT phosphorylation at <t>Ser473;</t> ( C ) quantitative analysis for p70S6K phosphorylation; ( E ) quantitative analysis for ERK1/2 phosphorylation. Total AKT, total ERK1/2, and beta actin (β-actin) were detected and used as an internal control. Data are represented as mean ± SD of three independent experiments; # p
    Alphaease Fc Software, supplied by Alpha Innotech, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Image Search Results


    ( A and D ) Western blot analysis for the inhibitory effects of OXY on the phosphorylation of AKT, p70S6K, and ERK1/2 in cells treated with various concentrations of OXY (10, 20, and 40 μM) for 4 h before stimulation with IL-1β for 20 min; ( B ) quantitative analysis for AKT phosphorylation at Ser473; ( C ) quantitative analysis for p70S6K phosphorylation; ( E ) quantitative analysis for ERK1/2 phosphorylation. Total AKT, total ERK1/2, and beta actin (β-actin) were detected and used as an internal control. Data are represented as mean ± SD of three independent experiments; # p

    Journal: International Journal of Molecular Sciences

    Article Title: Oxyresveratrol Inhibits IL-1β-Induced Inflammation via Suppressing AKT and ERK1/2 Activation in Human Microglia, HMC3

    doi: 10.3390/ijms21176054

    Figure Lengend Snippet: ( A and D ) Western blot analysis for the inhibitory effects of OXY on the phosphorylation of AKT, p70S6K, and ERK1/2 in cells treated with various concentrations of OXY (10, 20, and 40 μM) for 4 h before stimulation with IL-1β for 20 min; ( B ) quantitative analysis for AKT phosphorylation at Ser473; ( C ) quantitative analysis for p70S6K phosphorylation; ( E ) quantitative analysis for ERK1/2 phosphorylation. Total AKT, total ERK1/2, and beta actin (β-actin) were detected and used as an internal control. Data are represented as mean ± SD of three independent experiments; # p

    Article Snippet: After blocked with 5% skim milk in a mixture of tris-buffered saline and Tween 20 (TBS-T) (0.02 M Tris-HCl, pH 7.6, 0.0137 M NaCl, and 0.05% Tween 20) (all reagents from Sigma-Aldrich, Saint Louis, MO, USA) at RT for 1 h, membranes were incubated with an appropriate primary antibody (Cell Signaling Technology, Boston, MA, USA) at 4 °C for 24 h. Primary antibodies used in this study included an anti-NF-κB p65 (D14E12) antibody (catalog number 8242), a mouse anti-IκBα (L35A5) antibody (catalog number 4814), a phosphospecific rabbit anti-p44/42 MAPK (Erk1/2) (Thr202/Tyr204) antibody (catalog number 4370), a mouse anti-p44/42 MAPK (Erk1/2) antibody (catalog number 9107), a phosphospecific mouse anti-p38 MAPK (Thr180/Tyr182) antibody (catalog number 9216), a rabbit anti-p38 MAPK (D13E1) antibody (catalog number 8690), a phosphospecific rabbit anti-SAPK/JNK (Thr183/Tyr185) antibody (catalog number 4668), a rabbit anti-SAPK/JNK antibody (catalog number 9252), a phosphospecific rabbit anti-AKT (Ser473) antibody (catalog number 4060), a mouse anti-AKT (40D4) antibody (catalog number 2920), a phosphospecific rabbit p70 S6 kinase (Thr389) antibody (catalog number 97596), or a mouse anti-β-actin antibody (catalog number MA1115) (Boster Biological Technology, Pleasanton, CA, USA).

    Techniques: Western Blot

    The effects of OXY on regulating AKT phosphorylation in cells stimulated with IL-1β. Representative images from immunofluorescence study showing AKT phosphorylation at Ser473 (green) of untreated cells ( a–c ), IL-1β-stimulated cells ( d–f ), IL-1β-stimulated cells with the presence of OXY at 40 μM ( g–i ), or DMSO ( j–l ). Nuclei (blue) were stained with DAPI. The micrographs were captured at 100× magnification, and data are representatives of three replicates.

    Journal: International Journal of Molecular Sciences

    Article Title: Oxyresveratrol Inhibits IL-1β-Induced Inflammation via Suppressing AKT and ERK1/2 Activation in Human Microglia, HMC3

    doi: 10.3390/ijms21176054

    Figure Lengend Snippet: The effects of OXY on regulating AKT phosphorylation in cells stimulated with IL-1β. Representative images from immunofluorescence study showing AKT phosphorylation at Ser473 (green) of untreated cells ( a–c ), IL-1β-stimulated cells ( d–f ), IL-1β-stimulated cells with the presence of OXY at 40 μM ( g–i ), or DMSO ( j–l ). Nuclei (blue) were stained with DAPI. The micrographs were captured at 100× magnification, and data are representatives of three replicates.

    Article Snippet: After blocked with 5% skim milk in a mixture of tris-buffered saline and Tween 20 (TBS-T) (0.02 M Tris-HCl, pH 7.6, 0.0137 M NaCl, and 0.05% Tween 20) (all reagents from Sigma-Aldrich, Saint Louis, MO, USA) at RT for 1 h, membranes were incubated with an appropriate primary antibody (Cell Signaling Technology, Boston, MA, USA) at 4 °C for 24 h. Primary antibodies used in this study included an anti-NF-κB p65 (D14E12) antibody (catalog number 8242), a mouse anti-IκBα (L35A5) antibody (catalog number 4814), a phosphospecific rabbit anti-p44/42 MAPK (Erk1/2) (Thr202/Tyr204) antibody (catalog number 4370), a mouse anti-p44/42 MAPK (Erk1/2) antibody (catalog number 9107), a phosphospecific mouse anti-p38 MAPK (Thr180/Tyr182) antibody (catalog number 9216), a rabbit anti-p38 MAPK (D13E1) antibody (catalog number 8690), a phosphospecific rabbit anti-SAPK/JNK (Thr183/Tyr185) antibody (catalog number 4668), a rabbit anti-SAPK/JNK antibody (catalog number 9252), a phosphospecific rabbit anti-AKT (Ser473) antibody (catalog number 4060), a mouse anti-AKT (40D4) antibody (catalog number 2920), a phosphospecific rabbit p70 S6 kinase (Thr389) antibody (catalog number 97596), or a mouse anti-β-actin antibody (catalog number MA1115) (Boster Biological Technology, Pleasanton, CA, USA).

    Techniques: Immunofluorescence, Staining

    ( A ) Western blot analysis for the inhibitory effects of OXY on AKT and ERK1/2 phosphorylation in HMC3 after being treated with OXY (40 μM) for 4 h before stimulation with various concentrations of IL-1β (1, 10, and 100 ng/mL) for 20 min; ( B ) quantitative analysis for AKT phosphorylation at Ser473; ( C ) quantitative analysis for ERK1/2 phosphorylation. Total AKT and total ERK1/2 were detected and used as an internal control. ( D ) Western blot analysis detecting the phosphorylation status of AKT and ERK1/2 in response to IL-1β (1 ng/mL) for 20 min with or without the presence of 10 μM of LY294002 or 10 μM of U0126. The level of IL-6 ( E ) or MCP-1 ( F ) in the culture supernatants of HMC3 cells stimulated with IL-1β, with or without the presence of LY294002 or U0126. Data are represented as mean ± SD of three independent experiments; # p

    Journal: International Journal of Molecular Sciences

    Article Title: Oxyresveratrol Inhibits IL-1β-Induced Inflammation via Suppressing AKT and ERK1/2 Activation in Human Microglia, HMC3

    doi: 10.3390/ijms21176054

    Figure Lengend Snippet: ( A ) Western blot analysis for the inhibitory effects of OXY on AKT and ERK1/2 phosphorylation in HMC3 after being treated with OXY (40 μM) for 4 h before stimulation with various concentrations of IL-1β (1, 10, and 100 ng/mL) for 20 min; ( B ) quantitative analysis for AKT phosphorylation at Ser473; ( C ) quantitative analysis for ERK1/2 phosphorylation. Total AKT and total ERK1/2 were detected and used as an internal control. ( D ) Western blot analysis detecting the phosphorylation status of AKT and ERK1/2 in response to IL-1β (1 ng/mL) for 20 min with or without the presence of 10 μM of LY294002 or 10 μM of U0126. The level of IL-6 ( E ) or MCP-1 ( F ) in the culture supernatants of HMC3 cells stimulated with IL-1β, with or without the presence of LY294002 or U0126. Data are represented as mean ± SD of three independent experiments; # p

    Article Snippet: After blocked with 5% skim milk in a mixture of tris-buffered saline and Tween 20 (TBS-T) (0.02 M Tris-HCl, pH 7.6, 0.0137 M NaCl, and 0.05% Tween 20) (all reagents from Sigma-Aldrich, Saint Louis, MO, USA) at RT for 1 h, membranes were incubated with an appropriate primary antibody (Cell Signaling Technology, Boston, MA, USA) at 4 °C for 24 h. Primary antibodies used in this study included an anti-NF-κB p65 (D14E12) antibody (catalog number 8242), a mouse anti-IκBα (L35A5) antibody (catalog number 4814), a phosphospecific rabbit anti-p44/42 MAPK (Erk1/2) (Thr202/Tyr204) antibody (catalog number 4370), a mouse anti-p44/42 MAPK (Erk1/2) antibody (catalog number 9107), a phosphospecific mouse anti-p38 MAPK (Thr180/Tyr182) antibody (catalog number 9216), a rabbit anti-p38 MAPK (D13E1) antibody (catalog number 8690), a phosphospecific rabbit anti-SAPK/JNK (Thr183/Tyr185) antibody (catalog number 4668), a rabbit anti-SAPK/JNK antibody (catalog number 9252), a phosphospecific rabbit anti-AKT (Ser473) antibody (catalog number 4060), a mouse anti-AKT (40D4) antibody (catalog number 2920), a phosphospecific rabbit p70 S6 kinase (Thr389) antibody (catalog number 97596), or a mouse anti-β-actin antibody (catalog number MA1115) (Boster Biological Technology, Pleasanton, CA, USA).

    Techniques: Western Blot