P-172 Search Results


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  • 98
    Cell Signaling Technology Inc anti thr p 172 ampk
    Anti Thr P 172 Ampk, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 98/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    90
    InVivo BioTech Services mouse anti phosphotyrosine py72
    Mouse Anti Phosphotyrosine Py72, supplied by InVivo BioTech Services, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/mouse anti phosphotyrosine py72/product/InVivo BioTech Services
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    85
    InVivo BioTech Services mouse anti phospho tyrosine py72
    EGF-induced EGFR-QG-mCitrine phosphorylation and anisotropy upon PTP inhibition by PV. ( A) COS–7 cells expressing EGFR-mCitrine or EGFR-QG-mCitrine were stimulated with EGF (100 ng/ml). The collected lysates were used in western blot analysis and probed with the generic phospho tyrosine antibody <t>PY72</t> and anti-GFP (EGFR-mCitrine). Anti-tubulin was used as a loading control. The bar diagram shows the relative phosphorylation of EGFR-mCitrine and EGFR-QG-mCitrine for the indicated time points (mean ± SEM, n=3). ( B ) EGFR-QG-mCitrine anisotropy upon PV treatment. Representative fluorescence images of COS–7 cells co-expressing EGFR-QG-mCitrine and PTB-mCherry and the corresponding anisotropy images of EGFR-QG-mCitrine. Upper row: Pre-stimulation, lower row: 5-min post-stimulation with 0.33 mM PV. Scale bar: 10 μm. EGFR, epidermal growth factor receptor; PTB, phosphotyrosine-binding domain PV,pervanadate; SEM, standard error of the mean. DOI: http://dx.doi.org/10.7554/eLife.12223.009
    Mouse Anti Phospho Tyrosine Py72, supplied by InVivo BioTech Services, used in various techniques. Bioz Stars score: 85/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/mouse anti phospho tyrosine py72/product/InVivo BioTech Services
    Average 85 stars, based on 1 article reviews
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    Image Search Results


    EGF-induced EGFR-QG-mCitrine phosphorylation and anisotropy upon PTP inhibition by PV. ( A) COS–7 cells expressing EGFR-mCitrine or EGFR-QG-mCitrine were stimulated with EGF (100 ng/ml). The collected lysates were used in western blot analysis and probed with the generic phospho tyrosine antibody PY72 and anti-GFP (EGFR-mCitrine). Anti-tubulin was used as a loading control. The bar diagram shows the relative phosphorylation of EGFR-mCitrine and EGFR-QG-mCitrine for the indicated time points (mean ± SEM, n=3). ( B ) EGFR-QG-mCitrine anisotropy upon PV treatment. Representative fluorescence images of COS–7 cells co-expressing EGFR-QG-mCitrine and PTB-mCherry and the corresponding anisotropy images of EGFR-QG-mCitrine. Upper row: Pre-stimulation, lower row: 5-min post-stimulation with 0.33 mM PV. Scale bar: 10 μm. EGFR, epidermal growth factor receptor; PTB, phosphotyrosine-binding domain PV,pervanadate; SEM, standard error of the mean. DOI: http://dx.doi.org/10.7554/eLife.12223.009

    Journal: eLife

    Article Title: EGF-dependent re-routing of vesicular recycling switches spontaneous phosphorylation suppression to EGFR signaling

    doi: 10.7554/eLife.12223

    Figure Lengend Snippet: EGF-induced EGFR-QG-mCitrine phosphorylation and anisotropy upon PTP inhibition by PV. ( A) COS–7 cells expressing EGFR-mCitrine or EGFR-QG-mCitrine were stimulated with EGF (100 ng/ml). The collected lysates were used in western blot analysis and probed with the generic phospho tyrosine antibody PY72 and anti-GFP (EGFR-mCitrine). Anti-tubulin was used as a loading control. The bar diagram shows the relative phosphorylation of EGFR-mCitrine and EGFR-QG-mCitrine for the indicated time points (mean ± SEM, n=3). ( B ) EGFR-QG-mCitrine anisotropy upon PV treatment. Representative fluorescence images of COS–7 cells co-expressing EGFR-QG-mCitrine and PTB-mCherry and the corresponding anisotropy images of EGFR-QG-mCitrine. Upper row: Pre-stimulation, lower row: 5-min post-stimulation with 0.33 mM PV. Scale bar: 10 μm. EGFR, epidermal growth factor receptor; PTB, phosphotyrosine-binding domain PV,pervanadate; SEM, standard error of the mean. DOI: http://dx.doi.org/10.7554/eLife.12223.009

    Article Snippet: Primary antibodies Mouse anti-HA (MMS-101P, Biolegend, San Diego, CA), mouse anti-phospho tyrosine (PY72) (P172.1, InVivo Biotech Services, Henningsdorf, Germany), rabbit anti-c-Cbl (sc-170, Santa Cruz Biotechnologies, Heidelberg, Germany), mouse monoclonal anti-α-Tubulin (Sigma Aldrich, St. Louis, MO); living colors rabbit anti-GFP (632593, Clontech, Mountain View, CA), living colors mouse anti-GFP (632681, Clontech, Mountain View, CA), mouse anti-Rab11 (610656, BD Biosciences, Heidelberg, Germany), rabbit anti-Rab11a (ab65200, Abcam), rabbit anti-Rab11 (3539, Cell Signaling Technology, Danvers, MA), rabbit EGFR (4267, Cell Signaling Technology, Danvers, MA), rabbit pY1045 (2237, Cell Signaling Technology, Danvers, MA), rabbit pY1068 (3777, Cell Signaling Technology, Danvers, MA), goat EGFR (AF231, R & D Systems, Minneapolis, MN), mouse pY845 (558381, BD Biosciences, Heidelberg, Germany), mouse anti-Rab5 (610281, BD Biosciences, Heidelberg, Germany), rabbit anti-phospho ERK-1/2 Thr/Tyr 202/204 (9101, Cell Signaling Technology, Danvers, MA), mouse anti-ERK1/2 (Ab366991, Abcam); rabbit anti phosphor-Akt Ser473 (9271, Cell Signaling Technology, Danvers, MA), mouse anti-Akt (pan) (2920, Cell Signaling Technology, Danvers, MA).

    Techniques: Inhibition, Expressing, Western Blot, Fluorescence, Binding Assay

    EGF-induced vesicular trafficking of EGFR-Y1045/Y1068F/Y1086F-mCitrine and c-Cbl-mediated degradation profiles of EGFR upon EGF stimulation. ( A ) Representative fluorescence images of COS–7 cells co-expressing the ubiquitination-impaired mutant EGFR-Y1045/1068/1086F-mCitrine, BFP-Rab11a, and c-Cbl-mCherry upon stimulation with EGF-Alexa647 (5 ng/ml). Merged images of EGF-Alexa647 (green) and BFP-Rab11a (red) are shown. Scale bar: 10 μm. ( B ) Lysates of COS–7 cells co-expressing EGFR-mCitrine or EGFR-Y1045F-mCitrine or EGFR-Y1045/1068/1086F-mCitrine and c-Cbl-mCherry and stimulated with EGF (100 ng/ml) for the indicated time points were used in western blot analysis. Blots were probed with the generic phospho tyrosine antibody PY72, anti-GFP (EGFR-mCitrine) and anti-c-Cbl. Anti-tubulin was used as a loading control. ( C ) Ratiometric quantification of total EGFR-mCitrine/tubulin for EGFR wild type and the ubiquitination-impaired EGFR mutants for the indicated time points (mean ± SEM, n=3 blots). EGFR, epidermal growth factor receptor; SEM, standard error of the mean. DOI: http://dx.doi.org/10.7554/eLife.12223.020

    Journal: eLife

    Article Title: EGF-dependent re-routing of vesicular recycling switches spontaneous phosphorylation suppression to EGFR signaling

    doi: 10.7554/eLife.12223

    Figure Lengend Snippet: EGF-induced vesicular trafficking of EGFR-Y1045/Y1068F/Y1086F-mCitrine and c-Cbl-mediated degradation profiles of EGFR upon EGF stimulation. ( A ) Representative fluorescence images of COS–7 cells co-expressing the ubiquitination-impaired mutant EGFR-Y1045/1068/1086F-mCitrine, BFP-Rab11a, and c-Cbl-mCherry upon stimulation with EGF-Alexa647 (5 ng/ml). Merged images of EGF-Alexa647 (green) and BFP-Rab11a (red) are shown. Scale bar: 10 μm. ( B ) Lysates of COS–7 cells co-expressing EGFR-mCitrine or EGFR-Y1045F-mCitrine or EGFR-Y1045/1068/1086F-mCitrine and c-Cbl-mCherry and stimulated with EGF (100 ng/ml) for the indicated time points were used in western blot analysis. Blots were probed with the generic phospho tyrosine antibody PY72, anti-GFP (EGFR-mCitrine) and anti-c-Cbl. Anti-tubulin was used as a loading control. ( C ) Ratiometric quantification of total EGFR-mCitrine/tubulin for EGFR wild type and the ubiquitination-impaired EGFR mutants for the indicated time points (mean ± SEM, n=3 blots). EGFR, epidermal growth factor receptor; SEM, standard error of the mean. DOI: http://dx.doi.org/10.7554/eLife.12223.020

    Article Snippet: Primary antibodies Mouse anti-HA (MMS-101P, Biolegend, San Diego, CA), mouse anti-phospho tyrosine (PY72) (P172.1, InVivo Biotech Services, Henningsdorf, Germany), rabbit anti-c-Cbl (sc-170, Santa Cruz Biotechnologies, Heidelberg, Germany), mouse monoclonal anti-α-Tubulin (Sigma Aldrich, St. Louis, MO); living colors rabbit anti-GFP (632593, Clontech, Mountain View, CA), living colors mouse anti-GFP (632681, Clontech, Mountain View, CA), mouse anti-Rab11 (610656, BD Biosciences, Heidelberg, Germany), rabbit anti-Rab11a (ab65200, Abcam), rabbit anti-Rab11 (3539, Cell Signaling Technology, Danvers, MA), rabbit EGFR (4267, Cell Signaling Technology, Danvers, MA), rabbit pY1045 (2237, Cell Signaling Technology, Danvers, MA), rabbit pY1068 (3777, Cell Signaling Technology, Danvers, MA), goat EGFR (AF231, R & D Systems, Minneapolis, MN), mouse pY845 (558381, BD Biosciences, Heidelberg, Germany), mouse anti-Rab5 (610281, BD Biosciences, Heidelberg, Germany), rabbit anti-phospho ERK-1/2 Thr/Tyr 202/204 (9101, Cell Signaling Technology, Danvers, MA), mouse anti-ERK1/2 (Ab366991, Abcam); rabbit anti phosphor-Akt Ser473 (9271, Cell Signaling Technology, Danvers, MA), mouse anti-Akt (pan) (2920, Cell Signaling Technology, Danvers, MA).

    Techniques: Fluorescence, Expressing, Mutagenesis, Western Blot