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  • 99
    Thermo Fisher dihydrorhodamine 123
    NeuP-derived neutrophils are functionally equivalent to neutrophils. ( a ) ROS production by NeuPs and neutrophils that were sorted and stimulated with or without complement and E. coli . ROS-producing cells were detected using <t>dihydrorhodamine</t> 123 (DHR123) and analyzed by flow cytometry. N-acetylcysteine (NAC) was treated while staining with DHR123 to show the negative staining. Data are representative of two independent experiments (mean ± SD of N = 3 mice/group). ( b ) ROS production by G-CSF-cultured NeuPs and neutrophils that were stimulated with or without E. coli . ROS-producing cells were detected as in ( a ). ( c ) The ex vivo phagocytic ability of NeuPs and neutrophils. NeuPs and neutrophils were sorted and incubated with FITC-conjugated microbeads. Cells taking up the beads were analyzed by flow cytometry. ( d ) The phagocytic ability of G-CSF–cultured NeuPs and neutrophils. Cells were incubated with tdTomato-expressing E. coli and analyzed by flow cytometry. ( b–d ) Data are representative of three independent experiments (mean ± SD of N = 3 mice/group). ( e ) Expression of DC markers, CD11c and class II, in NeuPs and neutrophils cultured with GM-CSF for 96 hours, assessed by flow cytometry (left panel). Expression of CD11c and class II in NeuPs and neutrophils cultured with G-CSF for 96 hours, assessed by flow cytometry (right panel). Data are representative of two independent experiments.
    Dihydrorhodamine 123, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 974 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    89
    Takeda samp8 mice
    Effects of DL-3- n -butylphthalide (DL-NBP) management on structural synaptic plasticity in the hippocampus of <t>SAMP8</t> mice. (A–C) Representative western blot images of synaptophysin (SYN) and postsynaptic density protein 95 (PSD95). (D,E) SYN and PSD95 mRNA levels in the SAMP8 hippocampus. Values were normalized to β-actin and the mRNAs were expressed as a percentage of the SAMR1 vehicle-treated samples. Data are presented as the mean ± standard error of the mean (SEM). ∗ p
    Samp8 Mice, supplied by Takeda, used in various techniques. Bioz Stars score: 89/100, based on 55 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    90
    Kamada anei r
    Effects of DL-3- n -butylphthalide (DL-NBP) management on structural synaptic plasticity in the hippocampus of <t>SAMP8</t> mice. (A–C) Representative western blot images of synaptophysin (SYN) and postsynaptic density protein 95 (PSD95). (D,E) SYN and PSD95 mRNA levels in the SAMP8 hippocampus. Values were normalized to β-actin and the mRNAs were expressed as a percentage of the SAMR1 vehicle-treated samples. Data are presented as the mean ± standard error of the mean (SEM). ∗ p
    Anei R, supplied by Kamada, used in various techniques. Bioz Stars score: 90/100, based on 10 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    88
    Angelini c ultrastructural changes
    Effects of DL-3- n -butylphthalide (DL-NBP) management on structural synaptic plasticity in the hippocampus of <t>SAMP8</t> mice. (A–C) Representative western blot images of synaptophysin (SYN) and postsynaptic density protein 95 (PSD95). (D,E) SYN and PSD95 mRNA levels in the SAMP8 hippocampus. Values were normalized to β-actin and the mRNAs were expressed as a percentage of the SAMR1 vehicle-treated samples. Data are presented as the mean ± standard error of the mean (SEM). ∗ p
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    99
    Thermo Fisher b27 supplement
    Effects of DL-3- n -butylphthalide (DL-NBP) management on structural synaptic plasticity in the hippocampus of <t>SAMP8</t> mice. (A–C) Representative western blot images of synaptophysin (SYN) and postsynaptic density protein 95 (PSD95). (D,E) SYN and PSD95 mRNA levels in the SAMP8 hippocampus. Values were normalized to β-actin and the mRNAs were expressed as a percentage of the SAMR1 vehicle-treated samples. Data are presented as the mean ± standard error of the mean (SEM). ∗ p
    B27 Supplement, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 17028 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    91
    Takeda chiba y
    Effects of DL-3- n -butylphthalide (DL-NBP) management on structural synaptic plasticity in the hippocampus of <t>SAMP8</t> mice. (A–C) Representative western blot images of synaptophysin (SYN) and postsynaptic density protein 95 (PSD95). (D,E) SYN and PSD95 mRNA levels in the SAMP8 hippocampus. Values were normalized to β-actin and the mRNAs were expressed as a percentage of the SAMR1 vehicle-treated samples. Data are presented as the mean ± standard error of the mean (SEM). ∗ p
    Chiba Y, supplied by Takeda, used in various techniques. Bioz Stars score: 91/100, based on 17 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    93
    The Jackson Laboratory pain iasp
    Effects of DL-3- n -butylphthalide (DL-NBP) management on structural synaptic plasticity in the hippocampus of <t>SAMP8</t> mice. (A–C) Representative western blot images of synaptophysin (SYN) and postsynaptic density protein 95 (PSD95). (D,E) SYN and PSD95 mRNA levels in the SAMP8 hippocampus. Values were normalized to β-actin and the mRNAs were expressed as a percentage of the SAMR1 vehicle-treated samples. Data are presented as the mean ± standard error of the mean (SEM). ∗ p
    Pain Iasp, supplied by The Jackson Laboratory, used in various techniques. Bioz Stars score: 93/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    91
    Polysciences inc fitc conjugated microbeads
    NeuP-derived neutrophils are functionally equivalent to neutrophils. ( a ) ROS production by NeuPs and neutrophils that were sorted and stimulated with or without complement and E. coli . ROS-producing cells were detected using dihydrorhodamine 123 (DHR123) and analyzed by flow cytometry. N-acetylcysteine (NAC) was treated while staining with DHR123 to show the negative staining. Data are representative of two independent experiments (mean ± SD of N = 3 mice/group). ( b ) ROS production by G-CSF-cultured NeuPs and neutrophils that were stimulated with or without E. coli . ROS-producing cells were detected as in ( a ). ( c ) The ex vivo phagocytic ability of NeuPs and neutrophils. NeuPs and neutrophils were sorted and incubated with <t>FITC-conjugated</t> <t>microbeads.</t> Cells taking up the beads were analyzed by flow cytometry. ( d ) The phagocytic ability of G-CSF–cultured NeuPs and neutrophils. Cells were incubated with tdTomato-expressing E. coli and analyzed by flow cytometry. ( b–d ) Data are representative of three independent experiments (mean ± SD of N = 3 mice/group). ( e ) Expression of DC markers, CD11c and class II, in NeuPs and neutrophils cultured with GM-CSF for 96 hours, assessed by flow cytometry (left panel). Expression of CD11c and class II in NeuPs and neutrophils cultured with G-CSF for 96 hours, assessed by flow cytometry (right panel). Data are representative of two independent experiments.
    Fitc Conjugated Microbeads, supplied by Polysciences inc, used in various techniques. Bioz Stars score: 91/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    91
    Kyocera inamori professorship
    NeuP-derived neutrophils are functionally equivalent to neutrophils. ( a ) ROS production by NeuPs and neutrophils that were sorted and stimulated with or without complement and E. coli . ROS-producing cells were detected using dihydrorhodamine 123 (DHR123) and analyzed by flow cytometry. N-acetylcysteine (NAC) was treated while staining with DHR123 to show the negative staining. Data are representative of two independent experiments (mean ± SD of N = 3 mice/group). ( b ) ROS production by G-CSF-cultured NeuPs and neutrophils that were stimulated with or without E. coli . ROS-producing cells were detected as in ( a ). ( c ) The ex vivo phagocytic ability of NeuPs and neutrophils. NeuPs and neutrophils were sorted and incubated with <t>FITC-conjugated</t> <t>microbeads.</t> Cells taking up the beads were analyzed by flow cytometry. ( d ) The phagocytic ability of G-CSF–cultured NeuPs and neutrophils. Cells were incubated with tdTomato-expressing E. coli and analyzed by flow cytometry. ( b–d ) Data are representative of three independent experiments (mean ± SD of N = 3 mice/group). ( e ) Expression of DC markers, CD11c and class II, in NeuPs and neutrophils cultured with GM-CSF for 96 hours, assessed by flow cytometry (left panel). Expression of CD11c and class II in NeuPs and neutrophils cultured with G-CSF for 96 hours, assessed by flow cytometry (right panel). Data are representative of two independent experiments.
    Inamori Professorship, supplied by Kyocera, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    86
    Iwaki America iwaki t down regulation
    NeuP-derived neutrophils are functionally equivalent to neutrophils. ( a ) ROS production by NeuPs and neutrophils that were sorted and stimulated with or without complement and E. coli . ROS-producing cells were detected using dihydrorhodamine 123 (DHR123) and analyzed by flow cytometry. N-acetylcysteine (NAC) was treated while staining with DHR123 to show the negative staining. Data are representative of two independent experiments (mean ± SD of N = 3 mice/group). ( b ) ROS production by G-CSF-cultured NeuPs and neutrophils that were stimulated with or without E. coli . ROS-producing cells were detected as in ( a ). ( c ) The ex vivo phagocytic ability of NeuPs and neutrophils. NeuPs and neutrophils were sorted and incubated with <t>FITC-conjugated</t> <t>microbeads.</t> Cells taking up the beads were analyzed by flow cytometry. ( d ) The phagocytic ability of G-CSF–cultured NeuPs and neutrophils. Cells were incubated with tdTomato-expressing E. coli and analyzed by flow cytometry. ( b–d ) Data are representative of three independent experiments (mean ± SD of N = 3 mice/group). ( e ) Expression of DC markers, CD11c and class II, in NeuPs and neutrophils cultured with GM-CSF for 96 hours, assessed by flow cytometry (left panel). Expression of CD11c and class II in NeuPs and neutrophils cultured with G-CSF for 96 hours, assessed by flow cytometry (right panel). Data are representative of two independent experiments.
    Iwaki T Down Regulation, supplied by Iwaki America, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    90
    Iwaki America matsushita t
    NeuP-derived neutrophils are functionally equivalent to neutrophils. ( a ) ROS production by NeuPs and neutrophils that were sorted and stimulated with or without complement and E. coli . ROS-producing cells were detected using dihydrorhodamine 123 (DHR123) and analyzed by flow cytometry. N-acetylcysteine (NAC) was treated while staining with DHR123 to show the negative staining. Data are representative of two independent experiments (mean ± SD of N = 3 mice/group). ( b ) ROS production by G-CSF-cultured NeuPs and neutrophils that were stimulated with or without E. coli . ROS-producing cells were detected as in ( a ). ( c ) The ex vivo phagocytic ability of NeuPs and neutrophils. NeuPs and neutrophils were sorted and incubated with <t>FITC-conjugated</t> <t>microbeads.</t> Cells taking up the beads were analyzed by flow cytometry. ( d ) The phagocytic ability of G-CSF–cultured NeuPs and neutrophils. Cells were incubated with tdTomato-expressing E. coli and analyzed by flow cytometry. ( b–d ) Data are representative of three independent experiments (mean ± SD of N = 3 mice/group). ( e ) Expression of DC markers, CD11c and class II, in NeuPs and neutrophils cultured with GM-CSF for 96 hours, assessed by flow cytometry (left panel). Expression of CD11c and class II in NeuPs and neutrophils cultured with G-CSF for 96 hours, assessed by flow cytometry (right panel). Data are representative of two independent experiments.
    Matsushita T, supplied by Iwaki America, used in various techniques. Bioz Stars score: 90/100, based on 14 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    99
    Thermo Fisher n2 medium
    NeuP-derived neutrophils are functionally equivalent to neutrophils. ( a ) ROS production by NeuPs and neutrophils that were sorted and stimulated with or without complement and E. coli . ROS-producing cells were detected using dihydrorhodamine 123 (DHR123) and analyzed by flow cytometry. N-acetylcysteine (NAC) was treated while staining with DHR123 to show the negative staining. Data are representative of two independent experiments (mean ± SD of N = 3 mice/group). ( b ) ROS production by G-CSF-cultured NeuPs and neutrophils that were stimulated with or without E. coli . ROS-producing cells were detected as in ( a ). ( c ) The ex vivo phagocytic ability of NeuPs and neutrophils. NeuPs and neutrophils were sorted and incubated with <t>FITC-conjugated</t> <t>microbeads.</t> Cells taking up the beads were analyzed by flow cytometry. ( d ) The phagocytic ability of G-CSF–cultured NeuPs and neutrophils. Cells were incubated with tdTomato-expressing E. coli and analyzed by flow cytometry. ( b–d ) Data are representative of three independent experiments (mean ± SD of N = 3 mice/group). ( e ) Expression of DC markers, CD11c and class II, in NeuPs and neutrophils cultured with GM-CSF for 96 hours, assessed by flow cytometry (left panel). Expression of CD11c and class II in NeuPs and neutrophils cultured with G-CSF for 96 hours, assessed by flow cytometry (right panel). Data are representative of two independent experiments.
    N2 Medium, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 846 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher n2 supplement
    NeuP-derived neutrophils are functionally equivalent to neutrophils. ( a ) ROS production by NeuPs and neutrophils that were sorted and stimulated with or without complement and E. coli . ROS-producing cells were detected using dihydrorhodamine 123 (DHR123) and analyzed by flow cytometry. N-acetylcysteine (NAC) was treated while staining with DHR123 to show the negative staining. Data are representative of two independent experiments (mean ± SD of N = 3 mice/group). ( b ) ROS production by G-CSF-cultured NeuPs and neutrophils that were stimulated with or without E. coli . ROS-producing cells were detected as in ( a ). ( c ) The ex vivo phagocytic ability of NeuPs and neutrophils. NeuPs and neutrophils were sorted and incubated with <t>FITC-conjugated</t> <t>microbeads.</t> Cells taking up the beads were analyzed by flow cytometry. ( d ) The phagocytic ability of G-CSF–cultured NeuPs and neutrophils. Cells were incubated with tdTomato-expressing E. coli and analyzed by flow cytometry. ( b–d ) Data are representative of three independent experiments (mean ± SD of N = 3 mice/group). ( e ) Expression of DC markers, CD11c and class II, in NeuPs and neutrophils cultured with GM-CSF for 96 hours, assessed by flow cytometry (left panel). Expression of CD11c and class II in NeuPs and neutrophils cultured with G-CSF for 96 hours, assessed by flow cytometry (right panel). Data are representative of two independent experiments.
    N2 Supplement, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 8437 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Qiagen quantitect reverse transcription kit
    NeuP-derived neutrophils are functionally equivalent to neutrophils. ( a ) ROS production by NeuPs and neutrophils that were sorted and stimulated with or without complement and E. coli . ROS-producing cells were detected using dihydrorhodamine 123 (DHR123) and analyzed by flow cytometry. N-acetylcysteine (NAC) was treated while staining with DHR123 to show the negative staining. Data are representative of two independent experiments (mean ± SD of N = 3 mice/group). ( b ) ROS production by G-CSF-cultured NeuPs and neutrophils that were stimulated with or without E. coli . ROS-producing cells were detected as in ( a ). ( c ) The ex vivo phagocytic ability of NeuPs and neutrophils. NeuPs and neutrophils were sorted and incubated with <t>FITC-conjugated</t> <t>microbeads.</t> Cells taking up the beads were analyzed by flow cytometry. ( d ) The phagocytic ability of G-CSF–cultured NeuPs and neutrophils. Cells were incubated with tdTomato-expressing E. coli and analyzed by flow cytometry. ( b–d ) Data are representative of three independent experiments (mean ± SD of N = 3 mice/group). ( e ) Expression of DC markers, CD11c and class II, in NeuPs and neutrophils cultured with GM-CSF for 96 hours, assessed by flow cytometry (left panel). Expression of CD11c and class II in NeuPs and neutrophils cultured with G-CSF for 96 hours, assessed by flow cytometry (right panel). Data are representative of two independent experiments.
    Quantitect Reverse Transcription Kit, supplied by Qiagen, used in various techniques. Bioz Stars score: 99/100, based on 44197 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Qiagen rneasy kit
    NeuP-derived neutrophils are functionally equivalent to neutrophils. ( a ) ROS production by NeuPs and neutrophils that were sorted and stimulated with or without complement and E. coli . ROS-producing cells were detected using dihydrorhodamine 123 (DHR123) and analyzed by flow cytometry. N-acetylcysteine (NAC) was treated while staining with DHR123 to show the negative staining. Data are representative of two independent experiments (mean ± SD of N = 3 mice/group). ( b ) ROS production by G-CSF-cultured NeuPs and neutrophils that were stimulated with or without E. coli . ROS-producing cells were detected as in ( a ). ( c ) The ex vivo phagocytic ability of NeuPs and neutrophils. NeuPs and neutrophils were sorted and incubated with <t>FITC-conjugated</t> <t>microbeads.</t> Cells taking up the beads were analyzed by flow cytometry. ( d ) The phagocytic ability of G-CSF–cultured NeuPs and neutrophils. Cells were incubated with tdTomato-expressing E. coli and analyzed by flow cytometry. ( b–d ) Data are representative of three independent experiments (mean ± SD of N = 3 mice/group). ( e ) Expression of DC markers, CD11c and class II, in NeuPs and neutrophils cultured with GM-CSF for 96 hours, assessed by flow cytometry (left panel). Expression of CD11c and class II in NeuPs and neutrophils cultured with G-CSF for 96 hours, assessed by flow cytometry (right panel). Data are representative of two independent experiments.
    Rneasy Kit, supplied by Qiagen, used in various techniques. Bioz Stars score: 99/100, based on 110557 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher sybr green pcr master mix
    NeuP-derived neutrophils are functionally equivalent to neutrophils. ( a ) ROS production by NeuPs and neutrophils that were sorted and stimulated with or without complement and E. coli . ROS-producing cells were detected using dihydrorhodamine 123 (DHR123) and analyzed by flow cytometry. N-acetylcysteine (NAC) was treated while staining with DHR123 to show the negative staining. Data are representative of two independent experiments (mean ± SD of N = 3 mice/group). ( b ) ROS production by G-CSF-cultured NeuPs and neutrophils that were stimulated with or without E. coli . ROS-producing cells were detected as in ( a ). ( c ) The ex vivo phagocytic ability of NeuPs and neutrophils. NeuPs and neutrophils were sorted and incubated with <t>FITC-conjugated</t> <t>microbeads.</t> Cells taking up the beads were analyzed by flow cytometry. ( d ) The phagocytic ability of G-CSF–cultured NeuPs and neutrophils. Cells were incubated with tdTomato-expressing E. coli and analyzed by flow cytometry. ( b–d ) Data are representative of three independent experiments (mean ± SD of N = 3 mice/group). ( e ) Expression of DC markers, CD11c and class II, in NeuPs and neutrophils cultured with GM-CSF for 96 hours, assessed by flow cytometry (left panel). Expression of CD11c and class II in NeuPs and neutrophils cultured with G-CSF for 96 hours, assessed by flow cytometry (right panel). Data are representative of two independent experiments.
    Sybr Green Pcr Master Mix, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 101787 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Iwaki America t elevated expression
    NeuP-derived neutrophils are functionally equivalent to neutrophils. ( a ) ROS production by NeuPs and neutrophils that were sorted and stimulated with or without complement and E. coli . ROS-producing cells were detected using dihydrorhodamine 123 (DHR123) and analyzed by flow cytometry. N-acetylcysteine (NAC) was treated while staining with DHR123 to show the negative staining. Data are representative of two independent experiments (mean ± SD of N = 3 mice/group). ( b ) ROS production by G-CSF-cultured NeuPs and neutrophils that were stimulated with or without E. coli . ROS-producing cells were detected as in ( a ). ( c ) The ex vivo phagocytic ability of NeuPs and neutrophils. NeuPs and neutrophils were sorted and incubated with <t>FITC-conjugated</t> <t>microbeads.</t> Cells taking up the beads were analyzed by flow cytometry. ( d ) The phagocytic ability of G-CSF–cultured NeuPs and neutrophils. Cells were incubated with tdTomato-expressing E. coli and analyzed by flow cytometry. ( b–d ) Data are representative of three independent experiments (mean ± SD of N = 3 mice/group). ( e ) Expression of DC markers, CD11c and class II, in NeuPs and neutrophils cultured with GM-CSF for 96 hours, assessed by flow cytometry (left panel). Expression of CD11c and class II in NeuPs and neutrophils cultured with G-CSF for 96 hours, assessed by flow cytometry (right panel). Data are representative of two independent experiments.
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    Takeda toshio takeda s legacy
    NeuP-derived neutrophils are functionally equivalent to neutrophils. ( a ) ROS production by NeuPs and neutrophils that were sorted and stimulated with or without complement and E. coli . ROS-producing cells were detected using dihydrorhodamine 123 (DHR123) and analyzed by flow cytometry. N-acetylcysteine (NAC) was treated while staining with DHR123 to show the negative staining. Data are representative of two independent experiments (mean ± SD of N = 3 mice/group). ( b ) ROS production by G-CSF-cultured NeuPs and neutrophils that were stimulated with or without E. coli . ROS-producing cells were detected as in ( a ). ( c ) The ex vivo phagocytic ability of NeuPs and neutrophils. NeuPs and neutrophils were sorted and incubated with <t>FITC-conjugated</t> <t>microbeads.</t> Cells taking up the beads were analyzed by flow cytometry. ( d ) The phagocytic ability of G-CSF–cultured NeuPs and neutrophils. Cells were incubated with tdTomato-expressing E. coli and analyzed by flow cytometry. ( b–d ) Data are representative of three independent experiments (mean ± SD of N = 3 mice/group). ( e ) Expression of DC markers, CD11c and class II, in NeuPs and neutrophils cultured with GM-CSF for 96 hours, assessed by flow cytometry (left panel). Expression of CD11c and class II in NeuPs and neutrophils cultured with G-CSF for 96 hours, assessed by flow cytometry (right panel). Data are representative of two independent experiments.
    Toshio Takeda S Legacy, supplied by Takeda, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher tri reagent solution
    NeuP-derived neutrophils are functionally equivalent to neutrophils. ( a ) ROS production by NeuPs and neutrophils that were sorted and stimulated with or without complement and E. coli . ROS-producing cells were detected using dihydrorhodamine 123 (DHR123) and analyzed by flow cytometry. N-acetylcysteine (NAC) was treated while staining with DHR123 to show the negative staining. Data are representative of two independent experiments (mean ± SD of N = 3 mice/group). ( b ) ROS production by G-CSF-cultured NeuPs and neutrophils that were stimulated with or without E. coli . ROS-producing cells were detected as in ( a ). ( c ) The ex vivo phagocytic ability of NeuPs and neutrophils. NeuPs and neutrophils were sorted and incubated with <t>FITC-conjugated</t> <t>microbeads.</t> Cells taking up the beads were analyzed by flow cytometry. ( d ) The phagocytic ability of G-CSF–cultured NeuPs and neutrophils. Cells were incubated with tdTomato-expressing E. coli and analyzed by flow cytometry. ( b–d ) Data are representative of three independent experiments (mean ± SD of N = 3 mice/group). ( e ) Expression of DC markers, CD11c and class II, in NeuPs and neutrophils cultured with GM-CSF for 96 hours, assessed by flow cytometry (left panel). Expression of CD11c and class II in NeuPs and neutrophils cultured with G-CSF for 96 hours, assessed by flow cytometry (right panel). Data are representative of two independent experiments.
    Tri Reagent Solution, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 2707 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher trizol
    NeuP-derived neutrophils are functionally equivalent to neutrophils. ( a ) ROS production by NeuPs and neutrophils that were sorted and stimulated with or without complement and E. coli . ROS-producing cells were detected using dihydrorhodamine 123 (DHR123) and analyzed by flow cytometry. N-acetylcysteine (NAC) was treated while staining with DHR123 to show the negative staining. Data are representative of two independent experiments (mean ± SD of N = 3 mice/group). ( b ) ROS production by G-CSF-cultured NeuPs and neutrophils that were stimulated with or without E. coli . ROS-producing cells were detected as in ( a ). ( c ) The ex vivo phagocytic ability of NeuPs and neutrophils. NeuPs and neutrophils were sorted and incubated with <t>FITC-conjugated</t> <t>microbeads.</t> Cells taking up the beads were analyzed by flow cytometry. ( d ) The phagocytic ability of G-CSF–cultured NeuPs and neutrophils. Cells were incubated with tdTomato-expressing E. coli and analyzed by flow cytometry. ( b–d ) Data are representative of three independent experiments (mean ± SD of N = 3 mice/group). ( e ) Expression of DC markers, CD11c and class II, in NeuPs and neutrophils cultured with GM-CSF for 96 hours, assessed by flow cytometry (left panel). Expression of CD11c and class II in NeuPs and neutrophils cultured with G-CSF for 96 hours, assessed by flow cytometry (right panel). Data are representative of two independent experiments.
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    Takeda yoshimatsu k
    NeuP-derived neutrophils are functionally equivalent to neutrophils. ( a ) ROS production by NeuPs and neutrophils that were sorted and stimulated with or without complement and E. coli . ROS-producing cells were detected using dihydrorhodamine 123 (DHR123) and analyzed by flow cytometry. N-acetylcysteine (NAC) was treated while staining with DHR123 to show the negative staining. Data are representative of two independent experiments (mean ± SD of N = 3 mice/group). ( b ) ROS production by G-CSF-cultured NeuPs and neutrophils that were stimulated with or without E. coli . ROS-producing cells were detected as in ( a ). ( c ) The ex vivo phagocytic ability of NeuPs and neutrophils. NeuPs and neutrophils were sorted and incubated with <t>FITC-conjugated</t> <t>microbeads.</t> Cells taking up the beads were analyzed by flow cytometry. ( d ) The phagocytic ability of G-CSF–cultured NeuPs and neutrophils. Cells were incubated with tdTomato-expressing E. coli and analyzed by flow cytometry. ( b–d ) Data are representative of three independent experiments (mean ± SD of N = 3 mice/group). ( e ) Expression of DC markers, CD11c and class II, in NeuPs and neutrophils cultured with GM-CSF for 96 hours, assessed by flow cytometry (left panel). Expression of CD11c and class II in NeuPs and neutrophils cultured with G-CSF for 96 hours, assessed by flow cytometry (right panel). Data are representative of two independent experiments.
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    Image Search Results


    NeuP-derived neutrophils are functionally equivalent to neutrophils. ( a ) ROS production by NeuPs and neutrophils that were sorted and stimulated with or without complement and E. coli . ROS-producing cells were detected using dihydrorhodamine 123 (DHR123) and analyzed by flow cytometry. N-acetylcysteine (NAC) was treated while staining with DHR123 to show the negative staining. Data are representative of two independent experiments (mean ± SD of N = 3 mice/group). ( b ) ROS production by G-CSF-cultured NeuPs and neutrophils that were stimulated with or without E. coli . ROS-producing cells were detected as in ( a ). ( c ) The ex vivo phagocytic ability of NeuPs and neutrophils. NeuPs and neutrophils were sorted and incubated with FITC-conjugated microbeads. Cells taking up the beads were analyzed by flow cytometry. ( d ) The phagocytic ability of G-CSF–cultured NeuPs and neutrophils. Cells were incubated with tdTomato-expressing E. coli and analyzed by flow cytometry. ( b–d ) Data are representative of three independent experiments (mean ± SD of N = 3 mice/group). ( e ) Expression of DC markers, CD11c and class II, in NeuPs and neutrophils cultured with GM-CSF for 96 hours, assessed by flow cytometry (left panel). Expression of CD11c and class II in NeuPs and neutrophils cultured with G-CSF for 96 hours, assessed by flow cytometry (right panel). Data are representative of two independent experiments.

    Journal: Scientific Reports

    Article Title: A late-lineage murine neutrophil precursor population exhibits dynamic changes during demand-adapted granulopoiesis

    doi: 10.1038/srep39804

    Figure Lengend Snippet: NeuP-derived neutrophils are functionally equivalent to neutrophils. ( a ) ROS production by NeuPs and neutrophils that were sorted and stimulated with or without complement and E. coli . ROS-producing cells were detected using dihydrorhodamine 123 (DHR123) and analyzed by flow cytometry. N-acetylcysteine (NAC) was treated while staining with DHR123 to show the negative staining. Data are representative of two independent experiments (mean ± SD of N = 3 mice/group). ( b ) ROS production by G-CSF-cultured NeuPs and neutrophils that were stimulated with or without E. coli . ROS-producing cells were detected as in ( a ). ( c ) The ex vivo phagocytic ability of NeuPs and neutrophils. NeuPs and neutrophils were sorted and incubated with FITC-conjugated microbeads. Cells taking up the beads were analyzed by flow cytometry. ( d ) The phagocytic ability of G-CSF–cultured NeuPs and neutrophils. Cells were incubated with tdTomato-expressing E. coli and analyzed by flow cytometry. ( b–d ) Data are representative of three independent experiments (mean ± SD of N = 3 mice/group). ( e ) Expression of DC markers, CD11c and class II, in NeuPs and neutrophils cultured with GM-CSF for 96 hours, assessed by flow cytometry (left panel). Expression of CD11c and class II in NeuPs and neutrophils cultured with G-CSF for 96 hours, assessed by flow cytometry (right panel). Data are representative of two independent experiments.

    Article Snippet: Cells were stained with 5 μM dihydrorhodamine 123 (Invitrogen).

    Techniques: Derivative Assay, Flow Cytometry, Cytometry, Staining, Negative Staining, Mouse Assay, Cell Culture, Ex Vivo, Incubation, Expressing

    Effects of DL-3- n -butylphthalide (DL-NBP) management on structural synaptic plasticity in the hippocampus of SAMP8 mice. (A–C) Representative western blot images of synaptophysin (SYN) and postsynaptic density protein 95 (PSD95). (D,E) SYN and PSD95 mRNA levels in the SAMP8 hippocampus. Values were normalized to β-actin and the mRNAs were expressed as a percentage of the SAMR1 vehicle-treated samples. Data are presented as the mean ± standard error of the mean (SEM). ∗ p

    Journal: Frontiers in Aging Neuroscience

    Article Title: Long-Term DL-3-n-Butylphthalide Treatment Alleviates Cognitive Impairment Correlate With Improving Synaptic Plasticity in SAMP8 Mice

    doi: 10.3389/fnagi.2018.00200

    Figure Lengend Snippet: Effects of DL-3- n -butylphthalide (DL-NBP) management on structural synaptic plasticity in the hippocampus of SAMP8 mice. (A–C) Representative western blot images of synaptophysin (SYN) and postsynaptic density protein 95 (PSD95). (D,E) SYN and PSD95 mRNA levels in the SAMP8 hippocampus. Values were normalized to β-actin and the mRNAs were expressed as a percentage of the SAMR1 vehicle-treated samples. Data are presented as the mean ± standard error of the mean (SEM). ∗ p

    Article Snippet: SAMP8 mice as a neuropathological model of accelerated brain aging and dementia: Toshio Takeda’s legacy and future directions.

    Techniques: Mouse Assay, Western Blot

    Effects of DL-3- n -butylphthalide (DL-NBP) treatment on brain-derived neurotrophic factor (BDNF)/tropomyosin receptor kinase B (TrkB) signaling in the hippocampus of SAMP8 mice. (A–E) Representative western blot images of BDNF, TrkB, cAMP-response-element-binding protein (CREB), and phosphorylated CREB (pCREB). (F,G) BDNF and CREB mRNA levels evaluated by real-time PCR. Values were normalized to β-actin and the mRNAs were expressed as a percentage of the SAMR1 vehicle-treated samples. Data are presented as the mean ± standard error of the mean (SEM). ∗ p

    Journal: Frontiers in Aging Neuroscience

    Article Title: Long-Term DL-3-n-Butylphthalide Treatment Alleviates Cognitive Impairment Correlate With Improving Synaptic Plasticity in SAMP8 Mice

    doi: 10.3389/fnagi.2018.00200

    Figure Lengend Snippet: Effects of DL-3- n -butylphthalide (DL-NBP) treatment on brain-derived neurotrophic factor (BDNF)/tropomyosin receptor kinase B (TrkB) signaling in the hippocampus of SAMP8 mice. (A–E) Representative western blot images of BDNF, TrkB, cAMP-response-element-binding protein (CREB), and phosphorylated CREB (pCREB). (F,G) BDNF and CREB mRNA levels evaluated by real-time PCR. Values were normalized to β-actin and the mRNAs were expressed as a percentage of the SAMR1 vehicle-treated samples. Data are presented as the mean ± standard error of the mean (SEM). ∗ p

    Article Snippet: SAMP8 mice as a neuropathological model of accelerated brain aging and dementia: Toshio Takeda’s legacy and future directions.

    Techniques: Derivative Assay, Mouse Assay, Western Blot, Binding Assay, Real-time Polymerase Chain Reaction

    NeuP-derived neutrophils are functionally equivalent to neutrophils. ( a ) ROS production by NeuPs and neutrophils that were sorted and stimulated with or without complement and E. coli . ROS-producing cells were detected using dihydrorhodamine 123 (DHR123) and analyzed by flow cytometry. N-acetylcysteine (NAC) was treated while staining with DHR123 to show the negative staining. Data are representative of two independent experiments (mean ± SD of N = 3 mice/group). ( b ) ROS production by G-CSF-cultured NeuPs and neutrophils that were stimulated with or without E. coli . ROS-producing cells were detected as in ( a ). ( c ) The ex vivo phagocytic ability of NeuPs and neutrophils. NeuPs and neutrophils were sorted and incubated with FITC-conjugated microbeads. Cells taking up the beads were analyzed by flow cytometry. ( d ) The phagocytic ability of G-CSF–cultured NeuPs and neutrophils. Cells were incubated with tdTomato-expressing E. coli and analyzed by flow cytometry. ( b–d ) Data are representative of three independent experiments (mean ± SD of N = 3 mice/group). ( e ) Expression of DC markers, CD11c and class II, in NeuPs and neutrophils cultured with GM-CSF for 96 hours, assessed by flow cytometry (left panel). Expression of CD11c and class II in NeuPs and neutrophils cultured with G-CSF for 96 hours, assessed by flow cytometry (right panel). Data are representative of two independent experiments.

    Journal: Scientific Reports

    Article Title: A late-lineage murine neutrophil precursor population exhibits dynamic changes during demand-adapted granulopoiesis

    doi: 10.1038/srep39804

    Figure Lengend Snippet: NeuP-derived neutrophils are functionally equivalent to neutrophils. ( a ) ROS production by NeuPs and neutrophils that were sorted and stimulated with or without complement and E. coli . ROS-producing cells were detected using dihydrorhodamine 123 (DHR123) and analyzed by flow cytometry. N-acetylcysteine (NAC) was treated while staining with DHR123 to show the negative staining. Data are representative of two independent experiments (mean ± SD of N = 3 mice/group). ( b ) ROS production by G-CSF-cultured NeuPs and neutrophils that were stimulated with or without E. coli . ROS-producing cells were detected as in ( a ). ( c ) The ex vivo phagocytic ability of NeuPs and neutrophils. NeuPs and neutrophils were sorted and incubated with FITC-conjugated microbeads. Cells taking up the beads were analyzed by flow cytometry. ( d ) The phagocytic ability of G-CSF–cultured NeuPs and neutrophils. Cells were incubated with tdTomato-expressing E. coli and analyzed by flow cytometry. ( b–d ) Data are representative of three independent experiments (mean ± SD of N = 3 mice/group). ( e ) Expression of DC markers, CD11c and class II, in NeuPs and neutrophils cultured with GM-CSF for 96 hours, assessed by flow cytometry (left panel). Expression of CD11c and class II in NeuPs and neutrophils cultured with G-CSF for 96 hours, assessed by flow cytometry (right panel). Data are representative of two independent experiments.

    Article Snippet: Sorted cells were incubated with FITC-conjugated microbeads (Polysciences, FluoresbriteTM Plain YG 0.5 micron microspheres, 0.46 μm) for 30 min at 37 °C.

    Techniques: Derivative Assay, Flow Cytometry, Cytometry, Staining, Negative Staining, Mouse Assay, Cell Culture, Ex Vivo, Incubation, Expressing