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  • 95
    New England Biolabs plasmid dna
    GM1-ELISA to measure binding of LT 192 and LT 192 -Gly:Pro-STb proteins to GM1. Total proteins extracted from equivalent amounts of cells (determined by OD readings) of each strain (3030-2 [K88ac + LT + STb + ], 8017 <t>[1836-2/pBR322],</t> 8035 [1836-2 LT], 8221 [1836-2 LT 192 ], 8145 [1836-2 LT-Gly:Pro-STb], and 8488 [1836-2 LT 192 -Gly:Pro-STb]) were tested in GM1 binding using anti-CT as the primary antibody (1:5,000) and horseradish peroxidase-conjugated goat anti-rabbit IgG (1:5,000) as the secondary antibody. Optical densities, measured at 405 nm, showed significant differences for 3030-2, 8035, and 8221 strains ( P
    Plasmid Dna, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 95/100, based on 4477 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/plasmid dna/product/New England Biolabs
    Average 95 stars, based on 4477 article reviews
    Price from $9.99 to $1999.99
    plasmid dna - by Bioz Stars, 2020-07
    95/100 stars
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    93
    Millipore nnos
    Submucosal ganglion of the porcine stomach under physiological condition and after streptozotocine treatment immunoreactive to <t>nNOS,</t> <t>VIP</t> and GAL. A: Submucosal ganglion of the porcine corpus under physiological condition immunoreactive to Hu C/D; B: Submucosal ganglion of the porcine corpus under physiological condition immunoreactive to nNOS; C: Submucosal ganglion of the porcine corpus under physiological condition immunoreactive to Hu C/D and n NOS; D: Submucosal ganglion of the porcine corpus after streptozotocine treatment immunoreactive to Hu C/D; E: Submucosal ganglion of the porcine corpus after streptozotocine treatment immunoreactive to nNOS; F: Submucosal ganglion of the porcine corpus after streptozotocine treatment immunoreactive to Hu C/D and nNOS; G: Submucosal ganglion of the porcine corpus under physiological condition immunoreactive to Hu C/D; H: Submucosal ganglion of the porcine corpus under physiological condition immunoreactive to VIP; I: Submucosal ganglion of the porcine corpus under physiological condition immunoreactive to Hu C/D and VIP; J: Submucosal ganglion of the porcine corpus after streptozotocine treatment immunoreactive to Hu C/D; K: Submucosal ganglion of the porcine corpus after streptozotocine treatment immunoreactive to VIP; L: Submucosal ganglion of the porcine corpus after streptozotocine treatment immunoreactive to Hu C/D and VIP; M: Submucosal ganglion of the porcine corpus under physiological condition immunoreactive to Hu C/D; N: Submucosal ganglion of the porcine corpus under physiological condition immunoreactive to GAL; O: Submucosal ganglion of the porcine corpus under physiological condition immunoreactive to Hu C/D and GAL; P: Submucosal ganglion of the porcine corpus after streptozotocine treatment immunoreactive to Hu C/D; R: Submucosal ganglion of the porcine corpus after streptozotocine treatment immunoreactive to GAL; S: Submucosal ganglion of the porcine corpus after streptozotocine treatment immunoreactive to Hu C/D and GAL. The right column of the pictures shows the overlap of both stainings. Colocalization of both antigens in the studied cell bodies are indicated with arrows. nNOS: Neuronal isoform of nitric oxide synthase; VIP: Vasoactive intestinal peptide; GAL: Galanin; Hu C/D: Pan-neuronal marker.
    Nnos, supplied by Millipore, used in various techniques. Bioz Stars score: 93/100, based on 232 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/nnos/product/Millipore
    Average 93 stars, based on 232 article reviews
    Price from $9.99 to $1999.99
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    93/100 stars
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    96
    Millipore no synthase
    Group mean ± s.e.m. change in cutaneous vascular conductance (Δ%CVC max ) as a percentage of maximal vasodilatation between the control site and the <t>NO-synthase-inhibited</t> site across the rise in body core temperature (Δ T or ,°C)
    No Synthase, supplied by Millipore, used in various techniques. Bioz Stars score: 96/100, based on 115 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/no synthase/product/Millipore
    Average 96 stars, based on 115 article reviews
    Price from $9.99 to $1999.99
    no synthase - by Bioz Stars, 2020-07
    96/100 stars
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    Image Search Results


    GM1-ELISA to measure binding of LT 192 and LT 192 -Gly:Pro-STb proteins to GM1. Total proteins extracted from equivalent amounts of cells (determined by OD readings) of each strain (3030-2 [K88ac + LT + STb + ], 8017 [1836-2/pBR322], 8035 [1836-2 LT], 8221 [1836-2 LT 192 ], 8145 [1836-2 LT-Gly:Pro-STb], and 8488 [1836-2 LT 192 -Gly:Pro-STb]) were tested in GM1 binding using anti-CT as the primary antibody (1:5,000) and horseradish peroxidase-conjugated goat anti-rabbit IgG (1:5,000) as the secondary antibody. Optical densities, measured at 405 nm, showed significant differences for 3030-2, 8035, and 8221 strains ( P

    Journal: Clinical and Vaccine Immunology : CVI

    Article Title: Genetic Fusions of Heat-Labile Toxoid (LT) and Heat-Stable Toxin b (STb) of Porcine Enterotoxigenic Escherichia coli Elicit Protective Anti-LT and Anti-STb Antibodies ▿

    doi: 10.1128/CVI.00095-10

    Figure Lengend Snippet: GM1-ELISA to measure binding of LT 192 and LT 192 -Gly:Pro-STb proteins to GM1. Total proteins extracted from equivalent amounts of cells (determined by OD readings) of each strain (3030-2 [K88ac + LT + STb + ], 8017 [1836-2/pBR322], 8035 [1836-2 LT], 8221 [1836-2 LT 192 ], 8145 [1836-2 LT-Gly:Pro-STb], and 8488 [1836-2 LT 192 -Gly:Pro-STb]) were tested in GM1 binding using anti-CT as the primary antibody (1:5,000) and horseradish peroxidase-conjugated goat anti-rabbit IgG (1:5,000) as the secondary antibody. Optical densities, measured at 405 nm, showed significant differences for 3030-2, 8035, and 8221 strains ( P

    Article Snippet: Final PCR products (inserts) and vector pBR322 were digested with NheI and EagI restriction enzymes (New England Biolabs, Ipswich, MA) and ligated with T4 DNA ligase (Invitrogen) under standard conditions ( ).

    Techniques: Enzyme-linked Immunosorbent Assay, Binding Assay

    Detection of toxic activity in a porcine ligated-gut-loop assay. Individual ligated intestinal loops from the ileum and jejunum sections were inoculated with 2 × 10 9 CFU of overnight culture from strain 3030-2 (K88 + LT + STb + , a positive control), 8488 (1836-2 LT 192 -Gly:Pro-STb), 8221(1836-2 LT 192 ), 8017 (1836-2/pBR322, a negative control), or 8816 (1836-2 STb). Fluid accumulation was measured after 8 h postinoculation; the data are presented in the inserted table. Statistical analysis indicated that the fluid accumulation in loops incubated with strains 8488 and 8221 was not significantly different from that in loops incubated with the negative control 8017 strain ( P > 0.05), whereas fluid accumulated in the loops incubated with 8816 and 3030-2 was significantly different ( P

    Journal: Clinical and Vaccine Immunology : CVI

    Article Title: Genetic Fusions of Heat-Labile Toxoid (LT) and Heat-Stable Toxin b (STb) of Porcine Enterotoxigenic Escherichia coli Elicit Protective Anti-LT and Anti-STb Antibodies ▿

    doi: 10.1128/CVI.00095-10

    Figure Lengend Snippet: Detection of toxic activity in a porcine ligated-gut-loop assay. Individual ligated intestinal loops from the ileum and jejunum sections were inoculated with 2 × 10 9 CFU of overnight culture from strain 3030-2 (K88 + LT + STb + , a positive control), 8488 (1836-2 LT 192 -Gly:Pro-STb), 8221(1836-2 LT 192 ), 8017 (1836-2/pBR322, a negative control), or 8816 (1836-2 STb). Fluid accumulation was measured after 8 h postinoculation; the data are presented in the inserted table. Statistical analysis indicated that the fluid accumulation in loops incubated with strains 8488 and 8221 was not significantly different from that in loops incubated with the negative control 8017 strain ( P > 0.05), whereas fluid accumulated in the loops incubated with 8816 and 3030-2 was significantly different ( P

    Article Snippet: Final PCR products (inserts) and vector pBR322 were digested with NheI and EagI restriction enzymes (New England Biolabs, Ipswich, MA) and ligated with T4 DNA ligase (Invitrogen) under standard conditions ( ).

    Techniques: Activity Assay, Positive Control, Negative Control, Incubation

    The DNA-binding properties of β-Casein 197. a ProtParam analysis of predicted binding residues of β-casein 197, predicted binding residues was labeled with a red “+”. b Gel retardation assays. Binding was assayed by the migration of pDNA. Various concentrations of peptides were incubated with 5 µL of 25 µg mL −1 pBR322 vector from E. coli at 37 °C for 1 h, and then the reaction mixtures were applied to 0.7% agarose gel electrophoresis. Lane M DNA marker DL 10,000; Lane 1 5 µL pDNA as control; Lanes 2–6 mixture of pDNA and various concentrations of peptides (12.5, 25, 50, 100 and 500 µg mL −1 )

    Journal: AMB Express

    Article Title: Investigation into the antimicrobial action and mechanism of a novel endogenous peptide β-casein 197 from human milk

    doi: 10.1186/s13568-017-0409-y

    Figure Lengend Snippet: The DNA-binding properties of β-Casein 197. a ProtParam analysis of predicted binding residues of β-casein 197, predicted binding residues was labeled with a red “+”. b Gel retardation assays. Binding was assayed by the migration of pDNA. Various concentrations of peptides were incubated with 5 µL of 25 µg mL −1 pBR322 vector from E. coli at 37 °C for 1 h, and then the reaction mixtures were applied to 0.7% agarose gel electrophoresis. Lane M DNA marker DL 10,000; Lane 1 5 µL pDNA as control; Lanes 2–6 mixture of pDNA and various concentrations of peptides (12.5, 25, 50, 100 and 500 µg mL −1 )

    Article Snippet: DNA binding assay Gel-retardation experiments were performed using 5 µL of a 25 µg mL−1 pBR322 vector from E. coli (BioLabs, New England).

    Techniques: Binding Assay, Labeling, Electrophoretic Mobility Shift Assay, Migration, Incubation, Plasmid Preparation, Agarose Gel Electrophoresis, Marker

    Submucosal ganglion of the porcine stomach under physiological condition and after streptozotocine treatment immunoreactive to nNOS, VIP and GAL. A: Submucosal ganglion of the porcine corpus under physiological condition immunoreactive to Hu C/D; B: Submucosal ganglion of the porcine corpus under physiological condition immunoreactive to nNOS; C: Submucosal ganglion of the porcine corpus under physiological condition immunoreactive to Hu C/D and n NOS; D: Submucosal ganglion of the porcine corpus after streptozotocine treatment immunoreactive to Hu C/D; E: Submucosal ganglion of the porcine corpus after streptozotocine treatment immunoreactive to nNOS; F: Submucosal ganglion of the porcine corpus after streptozotocine treatment immunoreactive to Hu C/D and nNOS; G: Submucosal ganglion of the porcine corpus under physiological condition immunoreactive to Hu C/D; H: Submucosal ganglion of the porcine corpus under physiological condition immunoreactive to VIP; I: Submucosal ganglion of the porcine corpus under physiological condition immunoreactive to Hu C/D and VIP; J: Submucosal ganglion of the porcine corpus after streptozotocine treatment immunoreactive to Hu C/D; K: Submucosal ganglion of the porcine corpus after streptozotocine treatment immunoreactive to VIP; L: Submucosal ganglion of the porcine corpus after streptozotocine treatment immunoreactive to Hu C/D and VIP; M: Submucosal ganglion of the porcine corpus under physiological condition immunoreactive to Hu C/D; N: Submucosal ganglion of the porcine corpus under physiological condition immunoreactive to GAL; O: Submucosal ganglion of the porcine corpus under physiological condition immunoreactive to Hu C/D and GAL; P: Submucosal ganglion of the porcine corpus after streptozotocine treatment immunoreactive to Hu C/D; R: Submucosal ganglion of the porcine corpus after streptozotocine treatment immunoreactive to GAL; S: Submucosal ganglion of the porcine corpus after streptozotocine treatment immunoreactive to Hu C/D and GAL. The right column of the pictures shows the overlap of both stainings. Colocalization of both antigens in the studied cell bodies are indicated with arrows. nNOS: Neuronal isoform of nitric oxide synthase; VIP: Vasoactive intestinal peptide; GAL: Galanin; Hu C/D: Pan-neuronal marker.

    Journal: World Journal of Gastroenterology

    Article Title: Changes in expression of inhibitory substances in the intramural neurons of the stomach following streptozotocin- induced diabetes in the pig

    doi: 10.3748/wjg.v23.i33.6088

    Figure Lengend Snippet: Submucosal ganglion of the porcine stomach under physiological condition and after streptozotocine treatment immunoreactive to nNOS, VIP and GAL. A: Submucosal ganglion of the porcine corpus under physiological condition immunoreactive to Hu C/D; B: Submucosal ganglion of the porcine corpus under physiological condition immunoreactive to nNOS; C: Submucosal ganglion of the porcine corpus under physiological condition immunoreactive to Hu C/D and n NOS; D: Submucosal ganglion of the porcine corpus after streptozotocine treatment immunoreactive to Hu C/D; E: Submucosal ganglion of the porcine corpus after streptozotocine treatment immunoreactive to nNOS; F: Submucosal ganglion of the porcine corpus after streptozotocine treatment immunoreactive to Hu C/D and nNOS; G: Submucosal ganglion of the porcine corpus under physiological condition immunoreactive to Hu C/D; H: Submucosal ganglion of the porcine corpus under physiological condition immunoreactive to VIP; I: Submucosal ganglion of the porcine corpus under physiological condition immunoreactive to Hu C/D and VIP; J: Submucosal ganglion of the porcine corpus after streptozotocine treatment immunoreactive to Hu C/D; K: Submucosal ganglion of the porcine corpus after streptozotocine treatment immunoreactive to VIP; L: Submucosal ganglion of the porcine corpus after streptozotocine treatment immunoreactive to Hu C/D and VIP; M: Submucosal ganglion of the porcine corpus under physiological condition immunoreactive to Hu C/D; N: Submucosal ganglion of the porcine corpus under physiological condition immunoreactive to GAL; O: Submucosal ganglion of the porcine corpus under physiological condition immunoreactive to Hu C/D and GAL; P: Submucosal ganglion of the porcine corpus after streptozotocine treatment immunoreactive to Hu C/D; R: Submucosal ganglion of the porcine corpus after streptozotocine treatment immunoreactive to GAL; S: Submucosal ganglion of the porcine corpus after streptozotocine treatment immunoreactive to Hu C/D and GAL. The right column of the pictures shows the overlap of both stainings. Colocalization of both antigens in the studied cell bodies are indicated with arrows. nNOS: Neuronal isoform of nitric oxide synthase; VIP: Vasoactive intestinal peptide; GAL: Galanin; Hu C/D: Pan-neuronal marker.

    Article Snippet: The test was performed as follows: sections of the stomach were incubated with “working” dilution of the primary immunoserum, which had been previously pre-absorbed for 18 h at 37 °C with 20 μg of appropriate purified protein VIP (064-24, Phoenix Pharmaceutical), GAL (026-06, Phoenix Pharmaceutical) and nNOS (N3033, Sigma, St Louis, MO, United States).

    Techniques: Marker

    Group mean ± s.e.m. change in cutaneous vascular conductance (Δ%CVC max ) as a percentage of maximal vasodilatation between the control site and the NO-synthase-inhibited site across the rise in body core temperature (Δ T or ,°C)

    Journal:

    Article Title: Up-regulation of arginase activity contributes to attenuated reflex cutaneous vasodilatation in hypertensive humans

    doi: 10.1113/jphysiol.2007.128959

    Figure Lengend Snippet: Group mean ± s.e.m. change in cutaneous vascular conductance (Δ%CVC max ) as a percentage of maximal vasodilatation between the control site and the NO-synthase-inhibited site across the rise in body core temperature (Δ T or ,°C)

    Article Snippet: Following this period, microdialysis sites were randomly assigned to receive: (1) 10.0 m m N G. -nitro- l -arginine ( l -NAME) to inhibit NO production by NO-synthase ( ; ; ); (2) a combination of 5.0 m m ( S )-(2-boronoethyl)- l -cysteine-HCl (BEC) and 5.0 m m N ω -hydroxy-nor- l -arginine (nor-NOHA) to inhibit arginase ( ) (Calbiochem, San Diego, CA, USA); (3) 10.0 m m l -arginine (Sigma) to supplement the substrate for NO-synthase and arginase ( ); or (4) 5.0 m m BEC + 5.0 m m nor-NOHA + 10.0 m m l -arginine to inhibit arginase and supplement the substrate for NO-synthase and arginase.

    Techniques: