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  • 99
    Millipore ethylene glycol bis 2 aminoethylether n
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    Antibodies Inc sap97
    GluA1 phospho-Ser845 levels are higher and do not change after chem-LTP in Mecp2 KO mice. Shown is the altered expression of <t>SAP97</t> and EEA1 in Mecp2 KO mice. ( A ) Representative traces of fEPSPs before and after induction of forskolin-induced chem-LTP. Also shown is the time course of average fEPSPs during forskolin-induced chem-LTP. (Scale bar, 10 ms, 0.2 mV.) ( B and C ) Quantitative analysis of GluA1 phosopho-Ser845 ( B ) and phospho-Ser831 ( C ) in homogenates of hippocampal slices with or without forskolin/rolipram exposure, with (Fors/s) or without (Fors) afferent stimulation. ( Top ) Representative Western immunoblots. ( D ) Quantitative analysis of SAP97 protein ( Left ) and mRNA ( Right ) in homogenates of hippocampal samples. ( Top ) Representative Western immunoblots. ( E ) Quantitative analysis of EEA1 protein ( Left ) and mRNA ( Right ) in homogenates of hippocampal samples. ( Top ) Representative Western immunoblots. Data are presented as mean ± SEM; * P
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    FRANCE BIOIMAGING france bioimaging infrastructure
    GluA1 phospho-Ser845 levels are higher and do not change after chem-LTP in Mecp2 KO mice. Shown is the altered expression of <t>SAP97</t> and EEA1 in Mecp2 KO mice. ( A ) Representative traces of fEPSPs before and after induction of forskolin-induced chem-LTP. Also shown is the time course of average fEPSPs during forskolin-induced chem-LTP. (Scale bar, 10 ms, 0.2 mV.) ( B and C ) Quantitative analysis of GluA1 phosopho-Ser845 ( B ) and phospho-Ser831 ( C ) in homogenates of hippocampal slices with or without forskolin/rolipram exposure, with (Fors/s) or without (Fors) afferent stimulation. ( Top ) Representative Western immunoblots. ( D ) Quantitative analysis of SAP97 protein ( Left ) and mRNA ( Right ) in homogenates of hippocampal samples. ( Top ) Representative Western immunoblots. ( E ) Quantitative analysis of EEA1 protein ( Left ) and mRNA ( Right ) in homogenates of hippocampal samples. ( Top ) Representative Western immunoblots. Data are presented as mean ± SEM; * P
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    Millipore c05t
    GluA1 phospho-Ser845 levels are higher and do not change after chem-LTP in Mecp2 KO mice. Shown is the altered expression of <t>SAP97</t> and EEA1 in Mecp2 KO mice. ( A ) Representative traces of fEPSPs before and after induction of forskolin-induced chem-LTP. Also shown is the time course of average fEPSPs during forskolin-induced chem-LTP. (Scale bar, 10 ms, 0.2 mV.) ( B and C ) Quantitative analysis of GluA1 phosopho-Ser845 ( B ) and phospho-Ser831 ( C ) in homogenates of hippocampal slices with or without forskolin/rolipram exposure, with (Fors/s) or without (Fors) afferent stimulation. ( Top ) Representative Western immunoblots. ( D ) Quantitative analysis of SAP97 protein ( Left ) and mRNA ( Right ) in homogenates of hippocampal samples. ( Top ) Representative Western immunoblots. ( E ) Quantitative analysis of EEA1 protein ( Left ) and mRNA ( Right ) in homogenates of hippocampal samples. ( Top ) Representative Western immunoblots. Data are presented as mean ± SEM; * P
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    GluA1 phospho-Ser845 levels are higher and do not change after chem-LTP in Mecp2 KO mice. Shown is the altered expression of <t>SAP97</t> and EEA1 in Mecp2 KO mice. ( A ) Representative traces of fEPSPs before and after induction of forskolin-induced chem-LTP. Also shown is the time course of average fEPSPs during forskolin-induced chem-LTP. (Scale bar, 10 ms, 0.2 mV.) ( B and C ) Quantitative analysis of GluA1 phosopho-Ser845 ( B ) and phospho-Ser831 ( C ) in homogenates of hippocampal slices with or without forskolin/rolipram exposure, with (Fors/s) or without (Fors) afferent stimulation. ( Top ) Representative Western immunoblots. ( D ) Quantitative analysis of SAP97 protein ( Left ) and mRNA ( Right ) in homogenates of hippocampal samples. ( Top ) Representative Western immunoblots. ( E ) Quantitative analysis of EEA1 protein ( Left ) and mRNA ( Right ) in homogenates of hippocampal samples. ( Top ) Representative Western immunoblots. Data are presented as mean ± SEM; * P
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    GluA1 phospho-Ser845 levels are higher and do not change after chem-LTP in Mecp2 KO mice. Shown is the altered expression of <t>SAP97</t> and EEA1 in Mecp2 KO mice. ( A ) Representative traces of fEPSPs before and after induction of forskolin-induced chem-LTP. Also shown is the time course of average fEPSPs during forskolin-induced chem-LTP. (Scale bar, 10 ms, 0.2 mV.) ( B and C ) Quantitative analysis of GluA1 phosopho-Ser845 ( B ) and phospho-Ser831 ( C ) in homogenates of hippocampal slices with or without forskolin/rolipram exposure, with (Fors/s) or without (Fors) afferent stimulation. ( Top ) Representative Western immunoblots. ( D ) Quantitative analysis of SAP97 protein ( Left ) and mRNA ( Right ) in homogenates of hippocampal samples. ( Top ) Representative Western immunoblots. ( E ) Quantitative analysis of EEA1 protein ( Left ) and mRNA ( Right ) in homogenates of hippocampal samples. ( Top ) Representative Western immunoblots. Data are presented as mean ± SEM; * P
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    Millipore rabbit polyclonal anti tyrosine hydroxylase
    GluA1 phospho-Ser845 levels are higher and do not change after chem-LTP in Mecp2 KO mice. Shown is the altered expression of <t>SAP97</t> and EEA1 in Mecp2 KO mice. ( A ) Representative traces of fEPSPs before and after induction of forskolin-induced chem-LTP. Also shown is the time course of average fEPSPs during forskolin-induced chem-LTP. (Scale bar, 10 ms, 0.2 mV.) ( B and C ) Quantitative analysis of GluA1 phosopho-Ser845 ( B ) and phospho-Ser831 ( C ) in homogenates of hippocampal slices with or without forskolin/rolipram exposure, with (Fors/s) or without (Fors) afferent stimulation. ( Top ) Representative Western immunoblots. ( D ) Quantitative analysis of SAP97 protein ( Left ) and mRNA ( Right ) in homogenates of hippocampal samples. ( Top ) Representative Western immunoblots. ( E ) Quantitative analysis of EEA1 protein ( Left ) and mRNA ( Right ) in homogenates of hippocampal samples. ( Top ) Representative Western immunoblots. Data are presented as mean ± SEM; * P
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    GluA1 phospho-Ser845 levels are higher and do not change after chem-LTP in Mecp2 KO mice. Shown is the altered expression of <t>SAP97</t> and EEA1 in Mecp2 KO mice. ( A ) Representative traces of fEPSPs before and after induction of forskolin-induced chem-LTP. Also shown is the time course of average fEPSPs during forskolin-induced chem-LTP. (Scale bar, 10 ms, 0.2 mV.) ( B and C ) Quantitative analysis of GluA1 phosopho-Ser845 ( B ) and phospho-Ser831 ( C ) in homogenates of hippocampal slices with or without forskolin/rolipram exposure, with (Fors/s) or without (Fors) afferent stimulation. ( Top ) Representative Western immunoblots. ( D ) Quantitative analysis of SAP97 protein ( Left ) and mRNA ( Right ) in homogenates of hippocampal samples. ( Top ) Representative Western immunoblots. ( E ) Quantitative analysis of EEA1 protein ( Left ) and mRNA ( Right ) in homogenates of hippocampal samples. ( Top ) Representative Western immunoblots. Data are presented as mean ± SEM; * P
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    GluA1 phospho-Ser845 levels are higher and do not change after chem-LTP in Mecp2 KO mice. Shown is the altered expression of <t>SAP97</t> and EEA1 in Mecp2 KO mice. ( A ) Representative traces of fEPSPs before and after induction of forskolin-induced chem-LTP. Also shown is the time course of average fEPSPs during forskolin-induced chem-LTP. (Scale bar, 10 ms, 0.2 mV.) ( B and C ) Quantitative analysis of GluA1 phosopho-Ser845 ( B ) and phospho-Ser831 ( C ) in homogenates of hippocampal slices with or without forskolin/rolipram exposure, with (Fors/s) or without (Fors) afferent stimulation. ( Top ) Representative Western immunoblots. ( D ) Quantitative analysis of SAP97 protein ( Left ) and mRNA ( Right ) in homogenates of hippocampal samples. ( Top ) Representative Western immunoblots. ( E ) Quantitative analysis of EEA1 protein ( Left ) and mRNA ( Right ) in homogenates of hippocampal samples. ( Top ) Representative Western immunoblots. Data are presented as mean ± SEM; * P
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    GluA1 phospho-Ser845 levels are higher and do not change after chem-LTP in Mecp2 KO mice. Shown is the altered expression of <t>SAP97</t> and EEA1 in Mecp2 KO mice. ( A ) Representative traces of fEPSPs before and after induction of forskolin-induced chem-LTP. Also shown is the time course of average fEPSPs during forskolin-induced chem-LTP. (Scale bar, 10 ms, 0.2 mV.) ( B and C ) Quantitative analysis of GluA1 phosopho-Ser845 ( B ) and phospho-Ser831 ( C ) in homogenates of hippocampal slices with or without forskolin/rolipram exposure, with (Fors/s) or without (Fors) afferent stimulation. ( Top ) Representative Western immunoblots. ( D ) Quantitative analysis of SAP97 protein ( Left ) and mRNA ( Right ) in homogenates of hippocampal samples. ( Top ) Representative Western immunoblots. ( E ) Quantitative analysis of EEA1 protein ( Left ) and mRNA ( Right ) in homogenates of hippocampal samples. ( Top ) Representative Western immunoblots. Data are presented as mean ± SEM; * P
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    Santa Cruz Biotechnology mouse monoclonal anti p c jun ser63 73
    GluA1 phospho-Ser845 levels are higher and do not change after chem-LTP in Mecp2 KO mice. Shown is the altered expression of <t>SAP97</t> and EEA1 in Mecp2 KO mice. ( A ) Representative traces of fEPSPs before and after induction of forskolin-induced chem-LTP. Also shown is the time course of average fEPSPs during forskolin-induced chem-LTP. (Scale bar, 10 ms, 0.2 mV.) ( B and C ) Quantitative analysis of GluA1 phosopho-Ser845 ( B ) and phospho-Ser831 ( C ) in homogenates of hippocampal slices with or without forskolin/rolipram exposure, with (Fors/s) or without (Fors) afferent stimulation. ( Top ) Representative Western immunoblots. ( D ) Quantitative analysis of SAP97 protein ( Left ) and mRNA ( Right ) in homogenates of hippocampal samples. ( Top ) Representative Western immunoblots. ( E ) Quantitative analysis of EEA1 protein ( Left ) and mRNA ( Right ) in homogenates of hippocampal samples. ( Top ) Representative Western immunoblots. Data are presented as mean ± SEM; * P
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    GluA1 phospho-Ser845 levels are higher and do not change after chem-LTP in Mecp2 KO mice. Shown is the altered expression of <t>SAP97</t> and EEA1 in Mecp2 KO mice. ( A ) Representative traces of fEPSPs before and after induction of forskolin-induced chem-LTP. Also shown is the time course of average fEPSPs during forskolin-induced chem-LTP. (Scale bar, 10 ms, 0.2 mV.) ( B and C ) Quantitative analysis of GluA1 phosopho-Ser845 ( B ) and phospho-Ser831 ( C ) in homogenates of hippocampal slices with or without forskolin/rolipram exposure, with (Fors/s) or without (Fors) afferent stimulation. ( Top ) Representative Western immunoblots. ( D ) Quantitative analysis of SAP97 protein ( Left ) and mRNA ( Right ) in homogenates of hippocampal samples. ( Top ) Representative Western immunoblots. ( E ) Quantitative analysis of EEA1 protein ( Left ) and mRNA ( Right ) in homogenates of hippocampal samples. ( Top ) Representative Western immunoblots. Data are presented as mean ± SEM; * P
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    GluA1 phospho-Ser845 levels are higher and do not change after chem-LTP in Mecp2 KO mice. Shown is the altered expression of <t>SAP97</t> and EEA1 in Mecp2 KO mice. ( A ) Representative traces of fEPSPs before and after induction of forskolin-induced chem-LTP. Also shown is the time course of average fEPSPs during forskolin-induced chem-LTP. (Scale bar, 10 ms, 0.2 mV.) ( B and C ) Quantitative analysis of GluA1 phosopho-Ser845 ( B ) and phospho-Ser831 ( C ) in homogenates of hippocampal slices with or without forskolin/rolipram exposure, with (Fors/s) or without (Fors) afferent stimulation. ( Top ) Representative Western immunoblots. ( D ) Quantitative analysis of SAP97 protein ( Left ) and mRNA ( Right ) in homogenates of hippocampal samples. ( Top ) Representative Western immunoblots. ( E ) Quantitative analysis of EEA1 protein ( Left ) and mRNA ( Right ) in homogenates of hippocampal samples. ( Top ) Representative Western immunoblots. Data are presented as mean ± SEM; * P
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    Santa Cruz Biotechnology anti jnk3 4g6
    GluA1 phospho-Ser845 levels are higher and do not change after chem-LTP in Mecp2 KO mice. Shown is the altered expression of <t>SAP97</t> and EEA1 in Mecp2 KO mice. ( A ) Representative traces of fEPSPs before and after induction of forskolin-induced chem-LTP. Also shown is the time course of average fEPSPs during forskolin-induced chem-LTP. (Scale bar, 10 ms, 0.2 mV.) ( B and C ) Quantitative analysis of GluA1 phosopho-Ser845 ( B ) and phospho-Ser831 ( C ) in homogenates of hippocampal slices with or without forskolin/rolipram exposure, with (Fors/s) or without (Fors) afferent stimulation. ( Top ) Representative Western immunoblots. ( D ) Quantitative analysis of SAP97 protein ( Left ) and mRNA ( Right ) in homogenates of hippocampal samples. ( Top ) Representative Western immunoblots. ( E ) Quantitative analysis of EEA1 protein ( Left ) and mRNA ( Right ) in homogenates of hippocampal samples. ( Top ) Representative Western immunoblots. Data are presented as mean ± SEM; * P
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    Image Search Results


    GluA1 phospho-Ser845 levels are higher and do not change after chem-LTP in Mecp2 KO mice. Shown is the altered expression of SAP97 and EEA1 in Mecp2 KO mice. ( A ) Representative traces of fEPSPs before and after induction of forskolin-induced chem-LTP. Also shown is the time course of average fEPSPs during forskolin-induced chem-LTP. (Scale bar, 10 ms, 0.2 mV.) ( B and C ) Quantitative analysis of GluA1 phosopho-Ser845 ( B ) and phospho-Ser831 ( C ) in homogenates of hippocampal slices with or without forskolin/rolipram exposure, with (Fors/s) or without (Fors) afferent stimulation. ( Top ) Representative Western immunoblots. ( D ) Quantitative analysis of SAP97 protein ( Left ) and mRNA ( Right ) in homogenates of hippocampal samples. ( Top ) Representative Western immunoblots. ( E ) Quantitative analysis of EEA1 protein ( Left ) and mRNA ( Right ) in homogenates of hippocampal samples. ( Top ) Representative Western immunoblots. Data are presented as mean ± SEM; * P

    Journal: Proceedings of the National Academy of Sciences of the United States of America

    Article Title: Excitatory synapses are stronger in the hippocampus of Rett syndrome mice due to altered synaptic trafficking of AMPA-type glutamate receptors

    doi: 10.1073/pnas.1517244113

    Figure Lengend Snippet: GluA1 phospho-Ser845 levels are higher and do not change after chem-LTP in Mecp2 KO mice. Shown is the altered expression of SAP97 and EEA1 in Mecp2 KO mice. ( A ) Representative traces of fEPSPs before and after induction of forskolin-induced chem-LTP. Also shown is the time course of average fEPSPs during forskolin-induced chem-LTP. (Scale bar, 10 ms, 0.2 mV.) ( B and C ) Quantitative analysis of GluA1 phosopho-Ser845 ( B ) and phospho-Ser831 ( C ) in homogenates of hippocampal slices with or without forskolin/rolipram exposure, with (Fors/s) or without (Fors) afferent stimulation. ( Top ) Representative Western immunoblots. ( D ) Quantitative analysis of SAP97 protein ( Left ) and mRNA ( Right ) in homogenates of hippocampal samples. ( Top ) Representative Western immunoblots. ( E ) Quantitative analysis of EEA1 protein ( Left ) and mRNA ( Right ) in homogenates of hippocampal samples. ( Top ) Representative Western immunoblots. Data are presented as mean ± SEM; * P

    Article Snippet: Proteins were transferred to PVDF membrane and blocked with 5% nonfat milk in Tris-buffered saline with Tween 20 (TBST) (20 mM Tris, pH 7.6, 150 mM NaCl, and 0.1% Tween-20) for 1 h. Membranes were incubated with primary antibodies against MeCP2 (07-013, Millipore; M7443, Sigma-Aldrich), GluA1 (N terminus, MAB2263, Millipore), GluA1 (cytoplasmic domain, AB1504, Millipore), GluA1 phospho-Ser831 (AB5847, Millipore), GluA1 phospho-Ser845 (04-1073, Millipore), SAP97 (75-030, Antibodies Incorporated), or EEA1 (Cell Signaling), followed by corresponding HRP-conjugated secondary antibodies (Santa Cruz).

    Techniques: Mouse Assay, Expressing, Mass Spectrometry, Western Blot