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  • 95
    Gold Biotechnology Inc nourseothricin sulfate nat
    Materials
    Nourseothricin Sulfate Nat, supplied by Gold Biotechnology Inc, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    92
    Addgene inc pcdna3 flag mtet2 n500
    (A) FLAG-PROSER1 IP followed by mass spectrometry identifies the glycosyltransferase OGT and all three TET family proteins. (B) Western blot of FLAG IP from FLAG-HA-NeonGreen-PROSER1 ( FHNG-PROSER1 ) knock-in HEK293 cells confirming interaction of PROSER1 with OGT, TET1, TET2, and UTX. Wild-type (WT) HEK293 cells were used as an IP control. Nuclear extracts were used as input. Actin was used as a loading control for the inputs. The asterisk indicates the IgG heavy chain. (C) Top: Domain structure and constructs of mouse TET2. Blue: cysteine-rich dioxygenase (CD) domain. TET2 FL, full length construct. TET2 <t>N500,</t> N-terminal 500 amino acids (aa). TET2 N1041, N-terminal 1,041 aa. TET2 CD, TET2 cysteine–rich dioxygenase domain. Bottom: HEK293 cells were transiently transfected with a series of FLAG-tagged mouse TET2 constructs as shown on the top. WB of FLAG IPs from total cell lysates showing interaction of PROSER1 and OGT with TET2 FL and TET2 CD. Total cell lysates were used as inputs. Actin was used as a loading control for the inputs. The asterisk indicates the IgG heavy chain. (D) Top: Domain structure and constructs of the human TET2 cysteine–rich dioxygenase (CD) domain. Light blue, cysteine-rich domain. Red, double-stranded β-helix (DSBH1) domain 1. Olive, low-complexity insert (LCI) region. Purple, double-stranded β-helix (DSBH2) domain 2. Bottom: HEK293 cells were transiently transfected with a series of FLAG-tagged human TET2 constructs as shown on the top. WB of FLAG IPs from total cell lysates depicting interaction of PROSER1 and OGT with TET2 DSHB2. Total cell lysates were used as inputs. Actin was used as a loading control for the inputs. The asterisk indicates the IgG heavy chain. TET2 Cys-rich, TET2 LCI, and TET2 DSHB2 could not be detected in the inputs.
    Pcdna3 Flag Mtet2 N500, supplied by Addgene inc, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    86
    Japan Energy Corporation n 500
    (A) FLAG-PROSER1 IP followed by mass spectrometry identifies the glycosyltransferase OGT and all three TET family proteins. (B) Western blot of FLAG IP from FLAG-HA-NeonGreen-PROSER1 ( FHNG-PROSER1 ) knock-in HEK293 cells confirming interaction of PROSER1 with OGT, TET1, TET2, and UTX. Wild-type (WT) HEK293 cells were used as an IP control. Nuclear extracts were used as input. Actin was used as a loading control for the inputs. The asterisk indicates the IgG heavy chain. (C) Top: Domain structure and constructs of mouse TET2. Blue: cysteine-rich dioxygenase (CD) domain. TET2 FL, full length construct. TET2 <t>N500,</t> N-terminal 500 amino acids (aa). TET2 N1041, N-terminal 1,041 aa. TET2 CD, TET2 cysteine–rich dioxygenase domain. Bottom: HEK293 cells were transiently transfected with a series of FLAG-tagged mouse TET2 constructs as shown on the top. WB of FLAG IPs from total cell lysates showing interaction of PROSER1 and OGT with TET2 FL and TET2 CD. Total cell lysates were used as inputs. Actin was used as a loading control for the inputs. The asterisk indicates the IgG heavy chain. (D) Top: Domain structure and constructs of the human TET2 cysteine–rich dioxygenase (CD) domain. Light blue, cysteine-rich domain. Red, double-stranded β-helix (DSBH1) domain 1. Olive, low-complexity insert (LCI) region. Purple, double-stranded β-helix (DSBH2) domain 2. Bottom: HEK293 cells were transiently transfected with a series of FLAG-tagged human TET2 constructs as shown on the top. WB of FLAG IPs from total cell lysates depicting interaction of PROSER1 and OGT with TET2 DSHB2. Total cell lysates were used as inputs. Actin was used as a loading control for the inputs. The asterisk indicates the IgG heavy chain. TET2 Cys-rich, TET2 LCI, and TET2 DSHB2 could not be detected in the inputs.
    N 500, supplied by Japan Energy Corporation, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    86
    BUCHI AG nirflex n 500
    (A) FLAG-PROSER1 IP followed by mass spectrometry identifies the glycosyltransferase OGT and all three TET family proteins. (B) Western blot of FLAG IP from FLAG-HA-NeonGreen-PROSER1 ( FHNG-PROSER1 ) knock-in HEK293 cells confirming interaction of PROSER1 with OGT, TET1, TET2, and UTX. Wild-type (WT) HEK293 cells were used as an IP control. Nuclear extracts were used as input. Actin was used as a loading control for the inputs. The asterisk indicates the IgG heavy chain. (C) Top: Domain structure and constructs of mouse TET2. Blue: cysteine-rich dioxygenase (CD) domain. TET2 FL, full length construct. TET2 <t>N500,</t> N-terminal 500 amino acids (aa). TET2 N1041, N-terminal 1,041 aa. TET2 CD, TET2 cysteine–rich dioxygenase domain. Bottom: HEK293 cells were transiently transfected with a series of FLAG-tagged mouse TET2 constructs as shown on the top. WB of FLAG IPs from total cell lysates showing interaction of PROSER1 and OGT with TET2 FL and TET2 CD. Total cell lysates were used as inputs. Actin was used as a loading control for the inputs. The asterisk indicates the IgG heavy chain. (D) Top: Domain structure and constructs of the human TET2 cysteine–rich dioxygenase (CD) domain. Light blue, cysteine-rich domain. Red, double-stranded β-helix (DSBH1) domain 1. Olive, low-complexity insert (LCI) region. Purple, double-stranded β-helix (DSBH2) domain 2. Bottom: HEK293 cells were transiently transfected with a series of FLAG-tagged human TET2 constructs as shown on the top. WB of FLAG IPs from total cell lysates depicting interaction of PROSER1 and OGT with TET2 DSHB2. Total cell lysates were used as inputs. Actin was used as a loading control for the inputs. The asterisk indicates the IgG heavy chain. TET2 Cys-rich, TET2 LCI, and TET2 DSHB2 could not be detected in the inputs.
    Nirflex N 500, supplied by BUCHI AG, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    86
    Millipore n 500 kit
    (A) FLAG-PROSER1 IP followed by mass spectrometry identifies the glycosyltransferase OGT and all three TET family proteins. (B) Western blot of FLAG IP from FLAG-HA-NeonGreen-PROSER1 ( FHNG-PROSER1 ) knock-in HEK293 cells confirming interaction of PROSER1 with OGT, TET1, TET2, and UTX. Wild-type (WT) HEK293 cells were used as an IP control. Nuclear extracts were used as input. Actin was used as a loading control for the inputs. The asterisk indicates the IgG heavy chain. (C) Top: Domain structure and constructs of mouse TET2. Blue: cysteine-rich dioxygenase (CD) domain. TET2 FL, full length construct. TET2 <t>N500,</t> N-terminal 500 amino acids (aa). TET2 N1041, N-terminal 1,041 aa. TET2 CD, TET2 cysteine–rich dioxygenase domain. Bottom: HEK293 cells were transiently transfected with a series of FLAG-tagged mouse TET2 constructs as shown on the top. WB of FLAG IPs from total cell lysates showing interaction of PROSER1 and OGT with TET2 FL and TET2 CD. Total cell lysates were used as inputs. Actin was used as a loading control for the inputs. The asterisk indicates the IgG heavy chain. (D) Top: Domain structure and constructs of the human TET2 cysteine–rich dioxygenase (CD) domain. Light blue, cysteine-rich domain. Red, double-stranded β-helix (DSBH1) domain 1. Olive, low-complexity insert (LCI) region. Purple, double-stranded β-helix (DSBH2) domain 2. Bottom: HEK293 cells were transiently transfected with a series of FLAG-tagged human TET2 constructs as shown on the top. WB of FLAG IPs from total cell lysates depicting interaction of PROSER1 and OGT with TET2 DSHB2. Total cell lysates were used as inputs. Actin was used as a loading control for the inputs. The asterisk indicates the IgG heavy chain. TET2 Cys-rich, TET2 LCI, and TET2 DSHB2 could not be detected in the inputs.
    N 500 Kit, supplied by Millipore, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Image Search Results


    Materials

    Journal: Journal of visualized experiments : JoVE

    Article Title: Genome-wide Profiling of Transcription Factor-DNA Binding Interactions in Candida albicans : A Comprehensive CUT&RUN Method and Data Analysis Workflow

    doi: 10.3791/63655

    Figure Lengend Snippet: Materials

    Article Snippet: Nourseothricin sulfate (NAT) , Goldbio , N-500-2 , .

    Techniques: Cell Culture, Magnetic Beads, Electrophoresis, Staining, Software, Fluorescence, Microscopy, Nucleic Acid Electrophoresis, Ligation, Spectrophotometry, Multiplex Assay, Amplification, Plasmid Preparation, Negative Control, Protease Inhibitor

    (A) FLAG-PROSER1 IP followed by mass spectrometry identifies the glycosyltransferase OGT and all three TET family proteins. (B) Western blot of FLAG IP from FLAG-HA-NeonGreen-PROSER1 ( FHNG-PROSER1 ) knock-in HEK293 cells confirming interaction of PROSER1 with OGT, TET1, TET2, and UTX. Wild-type (WT) HEK293 cells were used as an IP control. Nuclear extracts were used as input. Actin was used as a loading control for the inputs. The asterisk indicates the IgG heavy chain. (C) Top: Domain structure and constructs of mouse TET2. Blue: cysteine-rich dioxygenase (CD) domain. TET2 FL, full length construct. TET2 N500, N-terminal 500 amino acids (aa). TET2 N1041, N-terminal 1,041 aa. TET2 CD, TET2 cysteine–rich dioxygenase domain. Bottom: HEK293 cells were transiently transfected with a series of FLAG-tagged mouse TET2 constructs as shown on the top. WB of FLAG IPs from total cell lysates showing interaction of PROSER1 and OGT with TET2 FL and TET2 CD. Total cell lysates were used as inputs. Actin was used as a loading control for the inputs. The asterisk indicates the IgG heavy chain. (D) Top: Domain structure and constructs of the human TET2 cysteine–rich dioxygenase (CD) domain. Light blue, cysteine-rich domain. Red, double-stranded β-helix (DSBH1) domain 1. Olive, low-complexity insert (LCI) region. Purple, double-stranded β-helix (DSBH2) domain 2. Bottom: HEK293 cells were transiently transfected with a series of FLAG-tagged human TET2 constructs as shown on the top. WB of FLAG IPs from total cell lysates depicting interaction of PROSER1 and OGT with TET2 DSHB2. Total cell lysates were used as inputs. Actin was used as a loading control for the inputs. The asterisk indicates the IgG heavy chain. TET2 Cys-rich, TET2 LCI, and TET2 DSHB2 could not be detected in the inputs.

    Journal: Life Science Alliance

    Article Title: PROSER1 mediates TET2 O-GlcNAcylation to regulate DNA demethylation on UTX-dependent enhancers and CpG islands

    doi: 10.26508/lsa.202101228

    Figure Lengend Snippet: (A) FLAG-PROSER1 IP followed by mass spectrometry identifies the glycosyltransferase OGT and all three TET family proteins. (B) Western blot of FLAG IP from FLAG-HA-NeonGreen-PROSER1 ( FHNG-PROSER1 ) knock-in HEK293 cells confirming interaction of PROSER1 with OGT, TET1, TET2, and UTX. Wild-type (WT) HEK293 cells were used as an IP control. Nuclear extracts were used as input. Actin was used as a loading control for the inputs. The asterisk indicates the IgG heavy chain. (C) Top: Domain structure and constructs of mouse TET2. Blue: cysteine-rich dioxygenase (CD) domain. TET2 FL, full length construct. TET2 N500, N-terminal 500 amino acids (aa). TET2 N1041, N-terminal 1,041 aa. TET2 CD, TET2 cysteine–rich dioxygenase domain. Bottom: HEK293 cells were transiently transfected with a series of FLAG-tagged mouse TET2 constructs as shown on the top. WB of FLAG IPs from total cell lysates showing interaction of PROSER1 and OGT with TET2 FL and TET2 CD. Total cell lysates were used as inputs. Actin was used as a loading control for the inputs. The asterisk indicates the IgG heavy chain. (D) Top: Domain structure and constructs of the human TET2 cysteine–rich dioxygenase (CD) domain. Light blue, cysteine-rich domain. Red, double-stranded β-helix (DSBH1) domain 1. Olive, low-complexity insert (LCI) region. Purple, double-stranded β-helix (DSBH2) domain 2. Bottom: HEK293 cells were transiently transfected with a series of FLAG-tagged human TET2 constructs as shown on the top. WB of FLAG IPs from total cell lysates depicting interaction of PROSER1 and OGT with TET2 DSHB2. Total cell lysates were used as inputs. Actin was used as a loading control for the inputs. The asterisk indicates the IgG heavy chain. TET2 Cys-rich, TET2 LCI, and TET2 DSHB2 could not be detected in the inputs.

    Article Snippet: pcDNA3-FLAG-mTET2-FL (full length), pcDNA3-FLAG-mTET2-N500 , pcDNA3-FLAG-mTET2-N1041 , and pcDNA3-FLAG-mTET2-CD were purchased from Addgene (deposited by the Xiong lab). pcDNA3-FLAG-hTET2-Cys-rich , pcDNA3-FLAG-hTET2-DSBH1 , pcDNA3-FLAG-hTET2-LCI , pcDNA3-FLAG-hTET2-DSBH2 , pcDNA3-FLAG-hTET2-DSBH2-N , and pcDNA3-FLAG-hTET2-DSBH2-C were cloned using the primers shown in Table S2. hTET2 fragments were PCR-amplified from cDNA of HEK293 cells and the pcDNA3 backbone was PCR-amplified from pcDNA3-Flag-hDPY30 obtained from Addgene (deposited by the Ge lab).

    Techniques: Mass Spectrometry, Western Blot, Knock-In, Construct, Transfection