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    New England Biolabs gdna
    CasKAS can be performed on pre-sheared <t>DNA.</t> CasKAS was performed in vitro using the EMX1 sgRNA, first, conventionally, by carrying out the CasKAS reaction, then isolating and shearing genomic DNA, and also by pre-shearing the DNA and carrying out the CasKAS reaction on the fragmented DNA. The concern in that case is that the presence of sticky ends containing Gs and unprotected from the action of the N 3 -kethoxal would lower the background. This problem can be addressed by carrying out end repair on the sheared DNA prior to the CasKAS reaction.
    Gdna, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/gdna/product/New England Biolabs
    Average 99 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    gdna - by Bioz Stars, 2021-06
    99/100 stars
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    95
    New England Biolabs genomic dna gdna
    CasKAS can be performed on pre-sheared <t>DNA.</t> CasKAS was performed in vitro using the EMX1 sgRNA, first, conventionally, by carrying out the CasKAS reaction, then isolating and shearing genomic DNA, and also by pre-shearing the DNA and carrying out the CasKAS reaction on the fragmented DNA. The concern in that case is that the presence of sticky ends containing Gs and unprotected from the action of the N 3 -kethoxal would lower the background. This problem can be addressed by carrying out end repair on the sheared DNA prior to the CasKAS reaction.
    Genomic Dna Gdna, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/genomic dna gdna/product/New England Biolabs
    Average 95 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    genomic dna gdna - by Bioz Stars, 2021-06
    95/100 stars
      Buy from Supplier

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    CasKAS can be performed on pre-sheared DNA. CasKAS was performed in vitro using the EMX1 sgRNA, first, conventionally, by carrying out the CasKAS reaction, then isolating and shearing genomic DNA, and also by pre-shearing the DNA and carrying out the CasKAS reaction on the fragmented DNA. The concern in that case is that the presence of sticky ends containing Gs and unprotected from the action of the N 3 -kethoxal would lower the background. This problem can be addressed by carrying out end repair on the sheared DNA prior to the CasKAS reaction.

    Journal: bioRxiv

    Article Title: Direct profiling of genome-wide dCas9 and Cas9 specificity using ssDNA mapping (CasKAS)

    doi: 10.1101/2021.04.16.440202

    Figure Lengend Snippet: CasKAS can be performed on pre-sheared DNA. CasKAS was performed in vitro using the EMX1 sgRNA, first, conventionally, by carrying out the CasKAS reaction, then isolating and shearing genomic DNA, and also by pre-shearing the DNA and carrying out the CasKAS reaction on the fragmented DNA. The concern in that case is that the presence of sticky ends containing Gs and unprotected from the action of the N 3 -kethoxal would lower the background. This problem can be addressed by carrying out end repair on the sheared DNA prior to the CasKAS reaction.

    Article Snippet: Cells were then pelleted by centrifuging at 500 g for 5 minutes at 4 ° C. Genomic DNA was then extracted using the Monarch gDNA Purification Kit (NEB T3010S) following the standard protocol but with elution using 175 µ L 25 mM K3 BO3 at pH 7.0.

    Techniques: In Vitro