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trichosporon loubieri strain atcc  (ATCC)
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ATCC trichosporon loubieri strain atcc
Growth of T. <t>loubieri</t> strain WW1C on with 3-oxo-C6-HSL as the sole energy source. Cell growth was monitored at OD 600 (on semi-log scale), determined over 24 h in ammonium chloride-replete (triangles) and depleted (squares) minimal medium supplemented with 3-oxo-C6-HSL. Residual growth in minimal medium without any carbon or nitrogen source is also shown (circles).
Trichosporon Loubieri Strain Atcc, supplied by ATCC, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Growth of T. loubieri strain WW1C on with 3-oxo-C6-HSL as the sole energy source. Cell growth was monitored at OD 600 (on semi-log scale), determined over 24 h in ammonium chloride-replete (triangles) and depleted (squares) minimal medium supplemented with 3-oxo-C6-HSL. Residual growth in minimal medium without any carbon or nitrogen source is also shown (circles).

Journal: Sensors (Basel, Switzerland)

Article Title: Degradation of Bacterial Quorum Sensing Signaling Molecules by the Microscopic Yeast Trichosporon loubieri Isolated from Tropical Wetland Waters

doi: 10.3390/s131012943

Figure Lengend Snippet: Growth of T. loubieri strain WW1C on with 3-oxo-C6-HSL as the sole energy source. Cell growth was monitored at OD 600 (on semi-log scale), determined over 24 h in ammonium chloride-replete (triangles) and depleted (squares) minimal medium supplemented with 3-oxo-C6-HSL. Residual growth in minimal medium without any carbon or nitrogen source is also shown (circles).

Article Snippet: GenBank accession numbers: Trichosporon loubieri (AB001759), Trichosporon dulcitum (AB001755), Trichosporon gracile (AB001756), Trichosporon multisporum (AB001764), Cryptococcus fragicola (AB035588), Trichosporon ovoides (AB001765), Bullera formosensis (AB072235), Kockovaella calophylli (AB042222), Trichosporon loubieri strain ATCC MYA-26 (AY101607), T richosporon loubieri strain CBS 7065 (AF444438), Trichosporon mycotoxinivorans (AJ601389), Trichosporon multisporum strain CBS2495 (AF414695), Trichosporon gracile strain CBS 8519 (AF444456), Trichosporonales sp. LM117 (EF060484), Trichosporon otae (AB180196), Cryptococcus laurentii strain WM 601 (EF568051), Cryptococcus curvatus strain ATCC 10567 (EU266558).

Techniques:

RRLC analysis of AHLs degradation after 0-, 6-, 12-, 18-, and 24-h incubation with T. loubieri strain WW1C.Degradation of ( a ) C4-HSL; ( b ) C6-HSL; ( c ) 3-oxo-C6-HSL; ( d ) C7-HSL; ( e) C8-HSL and ( f ) 3-oxo-C8-HSL by T. loubieri strain WW1C (square). Residual AHLs as measured based on calibration curve derived from calibration standards ranging from 0.0025 to 0.15 μg/μL. E. coli DH5α (circle) and incubation buffer (PBS, 100 mM, pH 6.5) (open triangle) served as negative controls. AHLs were incubated with T. loubieri strain WW1C cells, E. coli DH5α and PBS for 24 h, and extracted at 0-, 6-, 12-, 18- and 24-h, and analysed by RRLC.

Journal: Sensors (Basel, Switzerland)

Article Title: Degradation of Bacterial Quorum Sensing Signaling Molecules by the Microscopic Yeast Trichosporon loubieri Isolated from Tropical Wetland Waters

doi: 10.3390/s131012943

Figure Lengend Snippet: RRLC analysis of AHLs degradation after 0-, 6-, 12-, 18-, and 24-h incubation with T. loubieri strain WW1C.Degradation of ( a ) C4-HSL; ( b ) C6-HSL; ( c ) 3-oxo-C6-HSL; ( d ) C7-HSL; ( e) C8-HSL and ( f ) 3-oxo-C8-HSL by T. loubieri strain WW1C (square). Residual AHLs as measured based on calibration curve derived from calibration standards ranging from 0.0025 to 0.15 μg/μL. E. coli DH5α (circle) and incubation buffer (PBS, 100 mM, pH 6.5) (open triangle) served as negative controls. AHLs were incubated with T. loubieri strain WW1C cells, E. coli DH5α and PBS for 24 h, and extracted at 0-, 6-, 12-, 18- and 24-h, and analysed by RRLC.

Article Snippet: GenBank accession numbers: Trichosporon loubieri (AB001759), Trichosporon dulcitum (AB001755), Trichosporon gracile (AB001756), Trichosporon multisporum (AB001764), Cryptococcus fragicola (AB035588), Trichosporon ovoides (AB001765), Bullera formosensis (AB072235), Kockovaella calophylli (AB042222), Trichosporon loubieri strain ATCC MYA-26 (AY101607), T richosporon loubieri strain CBS 7065 (AF444438), Trichosporon mycotoxinivorans (AJ601389), Trichosporon multisporum strain CBS2495 (AF414695), Trichosporon gracile strain CBS 8519 (AF444456), Trichosporonales sp. LM117 (EF060484), Trichosporon otae (AB180196), Cryptococcus laurentii strain WM 601 (EF568051), Cryptococcus curvatus strain ATCC 10567 (EU266558).

Techniques: Incubation, Derivative Assay

Degradation of various AHLs by T. loubieri strain WW1C.

Journal: Sensors (Basel, Switzerland)

Article Title: Degradation of Bacterial Quorum Sensing Signaling Molecules by the Microscopic Yeast Trichosporon loubieri Isolated from Tropical Wetland Waters

doi: 10.3390/s131012943

Figure Lengend Snippet: Degradation of various AHLs by T. loubieri strain WW1C.

Article Snippet: GenBank accession numbers: Trichosporon loubieri (AB001759), Trichosporon dulcitum (AB001755), Trichosporon gracile (AB001756), Trichosporon multisporum (AB001764), Cryptococcus fragicola (AB035588), Trichosporon ovoides (AB001765), Bullera formosensis (AB072235), Kockovaella calophylli (AB042222), Trichosporon loubieri strain ATCC MYA-26 (AY101607), T richosporon loubieri strain CBS 7065 (AF444438), Trichosporon mycotoxinivorans (AJ601389), Trichosporon multisporum strain CBS2495 (AF414695), Trichosporon gracile strain CBS 8519 (AF444456), Trichosporonales sp. LM117 (EF060484), Trichosporon otae (AB180196), Cryptococcus laurentii strain WM 601 (EF568051), Cryptococcus curvatus strain ATCC 10567 (EU266558).

Techniques: Activity Assay

Analysis of C6-HSL after 0- and 24-h incubation with boiled and resting cells of T. loubieri strain WW1C. C6-HSL was incubated with boiled and resting cells of T. loubieri strain WW1C. Samples were withdrawn at 0 h (blue) and 24-h (purple), residual C6-HSL was detected by using the bioluminescent sensor strain E. coli (pSB401).

Journal: Sensors (Basel, Switzerland)

Article Title: Degradation of Bacterial Quorum Sensing Signaling Molecules by the Microscopic Yeast Trichosporon loubieri Isolated from Tropical Wetland Waters

doi: 10.3390/s131012943

Figure Lengend Snippet: Analysis of C6-HSL after 0- and 24-h incubation with boiled and resting cells of T. loubieri strain WW1C. C6-HSL was incubated with boiled and resting cells of T. loubieri strain WW1C. Samples were withdrawn at 0 h (blue) and 24-h (purple), residual C6-HSL was detected by using the bioluminescent sensor strain E. coli (pSB401).

Article Snippet: GenBank accession numbers: Trichosporon loubieri (AB001759), Trichosporon dulcitum (AB001755), Trichosporon gracile (AB001756), Trichosporon multisporum (AB001764), Cryptococcus fragicola (AB035588), Trichosporon ovoides (AB001765), Bullera formosensis (AB072235), Kockovaella calophylli (AB042222), Trichosporon loubieri strain ATCC MYA-26 (AY101607), T richosporon loubieri strain CBS 7065 (AF444438), Trichosporon mycotoxinivorans (AJ601389), Trichosporon multisporum strain CBS2495 (AF414695), Trichosporon gracile strain CBS 8519 (AF444456), Trichosporonales sp. LM117 (EF060484), Trichosporon otae (AB180196), Cryptococcus laurentii strain WM 601 (EF568051), Cryptococcus curvatus strain ATCC 10567 (EU266558).

Techniques: Incubation

Identification of degradation products of C6-HSL incubated with T. loubieri strain WW1C. C6-HSL incubated with T. loubieri strain WW1C cells for 0 h (0-h), 6 h (6-h), and samples withdrawn at 6 h and acidified (6-h acidified) to promote re-lactonisation. Bacterial names were labeled on the left of the figure. E. coli DH5α and extraction buffer (PBS 100 mM, pH 6.5) were used as the negative controls. Residual C6-HSL was detected by using CV026 and visualized as purple spot.

Journal: Sensors (Basel, Switzerland)

Article Title: Degradation of Bacterial Quorum Sensing Signaling Molecules by the Microscopic Yeast Trichosporon loubieri Isolated from Tropical Wetland Waters

doi: 10.3390/s131012943

Figure Lengend Snippet: Identification of degradation products of C6-HSL incubated with T. loubieri strain WW1C. C6-HSL incubated with T. loubieri strain WW1C cells for 0 h (0-h), 6 h (6-h), and samples withdrawn at 6 h and acidified (6-h acidified) to promote re-lactonisation. Bacterial names were labeled on the left of the figure. E. coli DH5α and extraction buffer (PBS 100 mM, pH 6.5) were used as the negative controls. Residual C6-HSL was detected by using CV026 and visualized as purple spot.

Article Snippet: GenBank accession numbers: Trichosporon loubieri (AB001759), Trichosporon dulcitum (AB001755), Trichosporon gracile (AB001756), Trichosporon multisporum (AB001764), Cryptococcus fragicola (AB035588), Trichosporon ovoides (AB001765), Bullera formosensis (AB072235), Kockovaella calophylli (AB042222), Trichosporon loubieri strain ATCC MYA-26 (AY101607), T richosporon loubieri strain CBS 7065 (AF444438), Trichosporon mycotoxinivorans (AJ601389), Trichosporon multisporum strain CBS2495 (AF414695), Trichosporon gracile strain CBS 8519 (AF444456), Trichosporonales sp. LM117 (EF060484), Trichosporon otae (AB180196), Cryptococcus laurentii strain WM 601 (EF568051), Cryptococcus curvatus strain ATCC 10567 (EU266558).

Techniques: Incubation, Labeling