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    OriGene adam10
    <t>ADAM10</t> expression in human renal tissues. ADAM10 expression (green) in renal biopsy samples from CKD patients (stages 2–3) was analyzed by immunofluorescence, with controls derived from living donor biopsies. Cell nuclei were stained with DAPI. Scale bar represents 50 µm. a – e Immunohistochemical staining of ADAM10 in the renal tissues of renal biopsy samples from CKD patients (stages 2–3). Original magnification, × 400
    Adam10, supplied by OriGene, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    ADAM10 expression in human renal tissues. ADAM10 expression (green) in renal biopsy samples from CKD patients (stages 2–3) was analyzed by immunofluorescence, with controls derived from living donor biopsies. Cell nuclei were stained with DAPI. Scale bar represents 50 µm. a – e Immunohistochemical staining of ADAM10 in the renal tissues of renal biopsy samples from CKD patients (stages 2–3). Original magnification, × 400

    Journal: International Urology and Nephrology

    Article Title: PAX2 may induce ADAM10 expression in renal tubular epithelial cells and contribute to epithelial-to-mesenchymal transition

    doi: 10.1007/s11255-018-1956-0

    Figure Lengend Snippet: ADAM10 expression in human renal tissues. ADAM10 expression (green) in renal biopsy samples from CKD patients (stages 2–3) was analyzed by immunofluorescence, with controls derived from living donor biopsies. Cell nuclei were stained with DAPI. Scale bar represents 50 µm. a – e Immunohistochemical staining of ADAM10 in the renal tissues of renal biopsy samples from CKD patients (stages 2–3). Original magnification, × 400

    Article Snippet: We then incubated slides at 4 °C overnight with primary antibodies, against ADAM10 (1:50; OriGene), E-cadherin (1:50; Abcam; ab76055), α-SMA (1:100; Abcam; ab5694), or PAX2 (1:50; Santa Cruz Biotechnology; sc-130387) for immunohistochemical examination, or ADAM10 (1:100; Diaclone, Besancon, France) for immunofluorescence staining.

    Techniques: Expressing, Immunofluorescence, Derivative Assay, Staining, Immunohistochemistry

    ADAM10 participates in UUO-induced renal fibrosis. a Immunohistochemical staining of E-cadherin, α-SMA, PAX2, and ADAM10 in the renal tissues of sham surgery and UUO rats. Original magnification, × 400. b Total kidney lysates from rats that underwent UUO or sham surgery rats were prepared. Protein expression was assessed by western blot relative to β-tubulin. Data represent mean ± SD ( n = 3). * P

    Journal: International Urology and Nephrology

    Article Title: PAX2 may induce ADAM10 expression in renal tubular epithelial cells and contribute to epithelial-to-mesenchymal transition

    doi: 10.1007/s11255-018-1956-0

    Figure Lengend Snippet: ADAM10 participates in UUO-induced renal fibrosis. a Immunohistochemical staining of E-cadherin, α-SMA, PAX2, and ADAM10 in the renal tissues of sham surgery and UUO rats. Original magnification, × 400. b Total kidney lysates from rats that underwent UUO or sham surgery rats were prepared. Protein expression was assessed by western blot relative to β-tubulin. Data represent mean ± SD ( n = 3). * P

    Article Snippet: We then incubated slides at 4 °C overnight with primary antibodies, against ADAM10 (1:50; OriGene), E-cadherin (1:50; Abcam; ab76055), α-SMA (1:100; Abcam; ab5694), or PAX2 (1:50; Santa Cruz Biotechnology; sc-130387) for immunohistochemical examination, or ADAM10 (1:100; Diaclone, Besancon, France) for immunofluorescence staining.

    Techniques: Immunohistochemistry, Staining, Expressing, Western Blot

    ADAM10 induces EMT. a E-cadherin (green) and α-SMA expression (red) were analyzed by immunofluorescence in HK-2 transfected with pRK5M–ADAM10 or empty vectors. HK-2 nuclei were stained with DAPI. Scale bars represent 50 µm. b Total cell lysates of HK-2 transfected with pRK5M–ADAM10 or empty vectors were prepared. Protein expression was normalized to β-tubulin. Data represent mean ± SD ( n = 3). c Messenger RNA was isolated from HK-2 transfected with pRK5M–ADAM10 or empty vectors. Data represent mean ± SD ( n = 3). * P

    Journal: International Urology and Nephrology

    Article Title: PAX2 may induce ADAM10 expression in renal tubular epithelial cells and contribute to epithelial-to-mesenchymal transition

    doi: 10.1007/s11255-018-1956-0

    Figure Lengend Snippet: ADAM10 induces EMT. a E-cadherin (green) and α-SMA expression (red) were analyzed by immunofluorescence in HK-2 transfected with pRK5M–ADAM10 or empty vectors. HK-2 nuclei were stained with DAPI. Scale bars represent 50 µm. b Total cell lysates of HK-2 transfected with pRK5M–ADAM10 or empty vectors were prepared. Protein expression was normalized to β-tubulin. Data represent mean ± SD ( n = 3). c Messenger RNA was isolated from HK-2 transfected with pRK5M–ADAM10 or empty vectors. Data represent mean ± SD ( n = 3). * P

    Article Snippet: We then incubated slides at 4 °C overnight with primary antibodies, against ADAM10 (1:50; OriGene), E-cadherin (1:50; Abcam; ab76055), α-SMA (1:100; Abcam; ab5694), or PAX2 (1:50; Santa Cruz Biotechnology; sc-130387) for immunohistochemical examination, or ADAM10 (1:100; Diaclone, Besancon, France) for immunofluorescence staining.

    Techniques: Expressing, Immunofluorescence, Transfection, Staining, Isolation

    Western blot indicating E-cadherin and α-SMA levels in PAX2-overexpressing NRK52E treated with the ADAM10 inhibitor, GI254023X (* P

    Journal: International Urology and Nephrology

    Article Title: PAX2 may induce ADAM10 expression in renal tubular epithelial cells and contribute to epithelial-to-mesenchymal transition

    doi: 10.1007/s11255-018-1956-0

    Figure Lengend Snippet: Western blot indicating E-cadherin and α-SMA levels in PAX2-overexpressing NRK52E treated with the ADAM10 inhibitor, GI254023X (* P

    Article Snippet: We then incubated slides at 4 °C overnight with primary antibodies, against ADAM10 (1:50; OriGene), E-cadherin (1:50; Abcam; ab76055), α-SMA (1:100; Abcam; ab5694), or PAX2 (1:50; Santa Cruz Biotechnology; sc-130387) for immunohistochemical examination, or ADAM10 (1:100; Diaclone, Besancon, France) for immunofluorescence staining.

    Techniques: Western Blot

    PAX2 directly binds the ADAM10 promoter. a A PAX2 consensus binding motif from the JASPAR CORE database ( http://jaspar.genereg.net/ ). b The putative PAX2 binding site (− 281 bp) within the ADAM10 promoter of rat renal tubular epithelial cells. c DNA sonication revealed that the majority of DNA had been sheared to fragments between 200 and 500 bp in length. d Results of a qChIP assay of the ADAM10 promoter region in NRK52E cells transfected with pGC–LV–PAX2 compared to those transfected with empty vectors. Data represent mean ± SD ( n = 3). IgG was used as a control

    Journal: International Urology and Nephrology

    Article Title: PAX2 may induce ADAM10 expression in renal tubular epithelial cells and contribute to epithelial-to-mesenchymal transition

    doi: 10.1007/s11255-018-1956-0

    Figure Lengend Snippet: PAX2 directly binds the ADAM10 promoter. a A PAX2 consensus binding motif from the JASPAR CORE database ( http://jaspar.genereg.net/ ). b The putative PAX2 binding site (− 281 bp) within the ADAM10 promoter of rat renal tubular epithelial cells. c DNA sonication revealed that the majority of DNA had been sheared to fragments between 200 and 500 bp in length. d Results of a qChIP assay of the ADAM10 promoter region in NRK52E cells transfected with pGC–LV–PAX2 compared to those transfected with empty vectors. Data represent mean ± SD ( n = 3). IgG was used as a control

    Article Snippet: We then incubated slides at 4 °C overnight with primary antibodies, against ADAM10 (1:50; OriGene), E-cadherin (1:50; Abcam; ab76055), α-SMA (1:100; Abcam; ab5694), or PAX2 (1:50; Santa Cruz Biotechnology; sc-130387) for immunohistochemical examination, or ADAM10 (1:100; Diaclone, Besancon, France) for immunofluorescence staining.

    Techniques: Binding Assay, Sonication, Transfection, Pyrolysis Gas Chromatography

    PAX2 influences ADAM10 expression. a Total cell lysates of NRK52E transfected with pGC–LV–PAX2, assessed by western blot. Protein levels were normalized to β-tubulin. Data represent mean ± SD ( n = 3). b ADAM10 expression (red) analyzed by immunofluorescence in NRK52E transfected with pGC–LV–PAX2 or empty vectors. The nuclei of NRK52E cells are stained with 4′,6-diamidino-2-phenylindole (DAPI). Scale bars represent 50 µm. c Messenger RNA isolated from NRK52E transfected with pGC–LV–PAX2 or empty vectors. Data represent mean ± SD ( n = 3). * P

    Journal: International Urology and Nephrology

    Article Title: PAX2 may induce ADAM10 expression in renal tubular epithelial cells and contribute to epithelial-to-mesenchymal transition

    doi: 10.1007/s11255-018-1956-0

    Figure Lengend Snippet: PAX2 influences ADAM10 expression. a Total cell lysates of NRK52E transfected with pGC–LV–PAX2, assessed by western blot. Protein levels were normalized to β-tubulin. Data represent mean ± SD ( n = 3). b ADAM10 expression (red) analyzed by immunofluorescence in NRK52E transfected with pGC–LV–PAX2 or empty vectors. The nuclei of NRK52E cells are stained with 4′,6-diamidino-2-phenylindole (DAPI). Scale bars represent 50 µm. c Messenger RNA isolated from NRK52E transfected with pGC–LV–PAX2 or empty vectors. Data represent mean ± SD ( n = 3). * P

    Article Snippet: We then incubated slides at 4 °C overnight with primary antibodies, against ADAM10 (1:50; OriGene), E-cadherin (1:50; Abcam; ab76055), α-SMA (1:100; Abcam; ab5694), or PAX2 (1:50; Santa Cruz Biotechnology; sc-130387) for immunohistochemical examination, or ADAM10 (1:100; Diaclone, Besancon, France) for immunofluorescence staining.

    Techniques: Expressing, Transfection, Pyrolysis Gas Chromatography, Western Blot, Immunofluorescence, Staining, Isolation