Journal: International Urology and Nephrology
Article Title: PAX2 may induce ADAM10 expression in renal tubular epithelial cells and contribute to epithelial-to-mesenchymal transition
Figure Lengend Snippet: PAX2 influences ADAM10 expression. a Total cell lysates of NRK52E transfected with pGC–LV–PAX2, assessed by western blot. Protein levels were normalized to β-tubulin. Data represent mean ± SD ( n = 3). b ADAM10 expression (red) analyzed by immunofluorescence in NRK52E transfected with pGC–LV–PAX2 or empty vectors. The nuclei of NRK52E cells are stained with 4′,6-diamidino-2-phenylindole (DAPI). Scale bars represent 50 µm. c Messenger RNA isolated from NRK52E transfected with pGC–LV–PAX2 or empty vectors. Data represent mean ± SD ( n = 3). * P
Article Snippet: We then incubated slides at 4 °C overnight with primary antibodies, against ADAM10 (1:50; OriGene), E-cadherin (1:50; Abcam; ab76055), α-SMA (1:100; Abcam; ab5694), or PAX2 (1:50; Santa Cruz Biotechnology; sc-130387) for immunohistochemical examination, or ADAM10 (1:100; Diaclone, Besancon, France) for immunofluorescence staining.
Techniques: Expressing, Transfection, Pyrolysis Gas Chromatography, Western Blot, Immunofluorescence, Staining, Isolation