MP-40 Search Results


mouse p40  (ATCC)
90
ATCC mouse p40
Cells plated at 50–60% confluence in 6-well plates were transfected with 1 μg each of <t>p40</t> and p35 cDNAs by LipofectAMINE Plus (Life Technologies, Inc.) as described under “Materials and Methods.” Twenty-four hours after transfection, cells were incubated with serum-free medium in the presence of 1 μg/ml of anti-mouse IL-12 p70. A, after 24 h, supernatants were used for nitrite assay. Data are mean ± S.D. of three different experiments. B, cell homogenates were electrophoresed, transferred onto nitrocellulose membranes, and immunoblotted with antibodies against mouse macrophage iNOS.
Mouse P40, supplied by ATCC, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/mouse p40/product/ATCC
Average 90 stars, based on 1 article reviews
Price from $9.99 to $1999.99
mouse p40 - by Bioz Stars, 2024-10
90/100 stars
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dualscope mp 40  (Helmut Fischer)
86
Helmut Fischer dualscope mp 40
Cells plated at 50–60% confluence in 6-well plates were transfected with 1 μg each of <t>p40</t> and p35 cDNAs by LipofectAMINE Plus (Life Technologies, Inc.) as described under “Materials and Methods.” Twenty-four hours after transfection, cells were incubated with serum-free medium in the presence of 1 μg/ml of anti-mouse IL-12 p70. A, after 24 h, supernatants were used for nitrite assay. Data are mean ± S.D. of three different experiments. B, cell homogenates were electrophoresed, transferred onto nitrocellulose membranes, and immunoblotted with antibodies against mouse macrophage iNOS.
Dualscope Mp 40, supplied by Helmut Fischer, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/dualscope mp 40/product/Helmut Fischer
Average 86 stars, based on 1 article reviews
Price from $9.99 to $1999.99
dualscope mp 40 - by Bioz Stars, 2024-10
86/100 stars
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mp 40 system  (MD Biosciences)
86
MD Biosciences mp 40 system
Cells plated at 50–60% confluence in 6-well plates were transfected with 1 μg each of <t>p40</t> and p35 cDNAs by LipofectAMINE Plus (Life Technologies, Inc.) as described under “Materials and Methods.” Twenty-four hours after transfection, cells were incubated with serum-free medium in the presence of 1 μg/ml of anti-mouse IL-12 p70. A, after 24 h, supernatants were used for nitrite assay. Data are mean ± S.D. of three different experiments. B, cell homogenates were electrophoresed, transferred onto nitrocellulose membranes, and immunoblotted with antibodies against mouse macrophage iNOS.
Mp 40 System, supplied by MD Biosciences, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/mp 40 system/product/MD Biosciences
Average 86 stars, based on 1 article reviews
Price from $9.99 to $1999.99
mp 40 system - by Bioz Stars, 2024-10
86/100 stars
  Buy from Supplier
intellivue mp 40  (Philips Healthcare)
86
Philips Healthcare intellivue mp 40
Cells plated at 50–60% confluence in 6-well plates were transfected with 1 μg each of <t>p40</t> and p35 cDNAs by LipofectAMINE Plus (Life Technologies, Inc.) as described under “Materials and Methods.” Twenty-four hours after transfection, cells were incubated with serum-free medium in the presence of 1 μg/ml of anti-mouse IL-12 p70. A, after 24 h, supernatants were used for nitrite assay. Data are mean ± S.D. of three different experiments. B, cell homogenates were electrophoresed, transferred onto nitrocellulose membranes, and immunoblotted with antibodies against mouse macrophage iNOS.
Intellivue Mp 40, supplied by Philips Healthcare, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/intellivue mp 40/product/Philips Healthcare
Average 86 stars, based on 1 article reviews
Price from $9.99 to $1999.99
intellivue mp 40 - by Bioz Stars, 2024-10
86/100 stars
  Buy from Supplier
intellivue mp 40 50  (Philips Healthcare)
86
Philips Healthcare intellivue mp 40 50
Cells plated at 50–60% confluence in 6-well plates were transfected with 1 μg each of <t>p40</t> and p35 cDNAs by LipofectAMINE Plus (Life Technologies, Inc.) as described under “Materials and Methods.” Twenty-four hours after transfection, cells were incubated with serum-free medium in the presence of 1 μg/ml of anti-mouse IL-12 p70. A, after 24 h, supernatants were used for nitrite assay. Data are mean ± S.D. of three different experiments. B, cell homogenates were electrophoresed, transferred onto nitrocellulose membranes, and immunoblotted with antibodies against mouse macrophage iNOS.
Intellivue Mp 40 50, supplied by Philips Healthcare, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/intellivue mp 40 50/product/Philips Healthcare
Average 86 stars, based on 1 article reviews
Price from $9.99 to $1999.99
intellivue mp 40 50 - by Bioz Stars, 2024-10
86/100 stars
  Buy from Supplier

Image Search Results


Cells plated at 50–60% confluence in 6-well plates were transfected with 1 μg each of p40 and p35 cDNAs by LipofectAMINE Plus (Life Technologies, Inc.) as described under “Materials and Methods.” Twenty-four hours after transfection, cells were incubated with serum-free medium in the presence of 1 μg/ml of anti-mouse IL-12 p70. A, after 24 h, supernatants were used for nitrite assay. Data are mean ± S.D. of three different experiments. B, cell homogenates were electrophoresed, transferred onto nitrocellulose membranes, and immunoblotted with antibodies against mouse macrophage iNOS.

Journal:

Article Title: Induction of Nitric-oxide Synthase and Activation of NF- κ B by Interleukin-12 p40 in Microglial Cells

doi: 10.1074/jbc.M008262200

Figure Lengend Snippet: Cells plated at 50–60% confluence in 6-well plates were transfected with 1 μg each of p40 and p35 cDNAs by LipofectAMINE Plus (Life Technologies, Inc.) as described under “Materials and Methods.” Twenty-four hours after transfection, cells were incubated with serum-free medium in the presence of 1 μg/ml of anti-mouse IL-12 p70. A, after 24 h, supernatants were used for nitrite assay. Data are mean ± S.D. of three different experiments. B, cell homogenates were electrophoresed, transferred onto nitrocellulose membranes, and immunoblotted with antibodies against mouse macrophage iNOS.

Article Snippet: Construction of Mouse p40 and p35 cDNA Expression Constructs Recombinant plasmids containing cDNA for mouse p40 (ATCC 87595) and p35 (ATCC 87596) in pBluescript SK+ were obtained from the ATCC.

Techniques: Transfection, Incubation, Nitration

Induction of NO production by IL-12 p70 and IL-12 p40 2 in mouse primary astrocytes, microglia, and macrophages Mouse peritoneal macrophages and primary astrocytes and microglia were cultured for 24 h in serum-free DMEM/F-12 with the listed reagents. Nitrite concentration in the supernatants was measured as described under “ Materials and Methods .” Data are mean ± S.D. of three different experiments.

Journal:

Article Title: Induction of Nitric-oxide Synthase and Activation of NF- κ B by Interleukin-12 p40 in Microglial Cells

doi: 10.1074/jbc.M008262200

Figure Lengend Snippet: Induction of NO production by IL-12 p70 and IL-12 p40 2 in mouse primary astrocytes, microglia, and macrophages Mouse peritoneal macrophages and primary astrocytes and microglia were cultured for 24 h in serum-free DMEM/F-12 with the listed reagents. Nitrite concentration in the supernatants was measured as described under “ Materials and Methods .” Data are mean ± S.D. of three different experiments.

Article Snippet: Construction of Mouse p40 and p35 cDNA Expression Constructs Recombinant plasmids containing cDNA for mouse p40 (ATCC 87595) and p35 (ATCC 87596) in pBluescript SK+ were obtained from the ATCC.

Techniques: Cell Culture, Concentration Assay

Cells plated at 50–60% confluence in 6-well plates were cotransfected with 1 μg of pNF-κB-Luc (an NF-κB-dependent reporter construct) and 1 µg of p40, p35, or the empty vector using the LipofectAMINE Plus method. All transfections included 50 ng/µg total DNA of pRL-TK. After 24 h of transfection, cells were incubated with serum-free medium for 24 h in the presence of 1 µg/ml of anti-mouse IL-12 p70. Firefly and Renilla luciferase activities were obtained by analyzing total cell extract as described under “Materials and Methods.”

Journal:

Article Title: Induction of Nitric-oxide Synthase and Activation of NF- κ B by Interleukin-12 p40 in Microglial Cells

doi: 10.1074/jbc.M008262200

Figure Lengend Snippet: Cells plated at 50–60% confluence in 6-well plates were cotransfected with 1 μg of pNF-κB-Luc (an NF-κB-dependent reporter construct) and 1 µg of p40, p35, or the empty vector using the LipofectAMINE Plus method. All transfections included 50 ng/µg total DNA of pRL-TK. After 24 h of transfection, cells were incubated with serum-free medium for 24 h in the presence of 1 µg/ml of anti-mouse IL-12 p70. Firefly and Renilla luciferase activities were obtained by analyzing total cell extract as described under “Materials and Methods.”

Article Snippet: Construction of Mouse p40 and p35 cDNA Expression Constructs Recombinant plasmids containing cDNA for mouse p40 (ATCC 87595) and p35 (ATCC 87596) in pBluescript SK+ were obtained from the ATCC.

Techniques: Construct, Plasmid Preparation, Transfection, Incubation, Luciferase