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  • 93
    Thermo Fisher mouse anti oct4
    Optimization and characterisation of neuruloids. a , SOX2 (neural progenitors-like cells) and T (primitive streak-like cells) expression in gastruloids and neuruloids with different starting ratio of ES and XEN cells (z-projection of whole mount immunostaining). Scale bars 100 µm. b , SOX2 and <t>OCT4</t> expression (immunostaining) in sections of neuruloids at 96 h (left, scale bars 100 µm) and cultured ESCs (right, scale bars 10 µm). a-b, Cells nuclei were stained with DAPI.
    Mouse Anti Oct4, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/mouse anti oct4/product/Thermo Fisher
    Average 93 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    mouse anti oct4 - by Bioz Stars, 2021-09
    93/100 stars
      Buy from Supplier

    86
    Thermo Fisher human pou5f1 oct3 4
    Optimization and characterisation of neuruloids. a , SOX2 (neural progenitors-like cells) and T (primitive streak-like cells) expression in gastruloids and neuruloids with different starting ratio of ES and XEN cells (z-projection of whole mount immunostaining). Scale bars 100 µm. b , SOX2 and <t>OCT4</t> expression (immunostaining) in sections of neuruloids at 96 h (left, scale bars 100 µm) and cultured ESCs (right, scale bars 10 µm). a-b, Cells nuclei were stained with DAPI.
    Human Pou5f1 Oct3 4, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/human pou5f1 oct3 4/product/Thermo Fisher
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    human pou5f1 oct3 4 - by Bioz Stars, 2021-09
    86/100 stars
      Buy from Supplier

    Image Search Results


    Optimization and characterisation of neuruloids. a , SOX2 (neural progenitors-like cells) and T (primitive streak-like cells) expression in gastruloids and neuruloids with different starting ratio of ES and XEN cells (z-projection of whole mount immunostaining). Scale bars 100 µm. b , SOX2 and OCT4 expression (immunostaining) in sections of neuruloids at 96 h (left, scale bars 100 µm) and cultured ESCs (right, scale bars 10 µm). a-b, Cells nuclei were stained with DAPI.

    Journal: bioRxiv

    Article Title: Early neurulation recapitulated in assemblies of embryonic and extraembryonic cells

    doi: 10.1101/2020.02.13.947655

    Figure Lengend Snippet: Optimization and characterisation of neuruloids. a , SOX2 (neural progenitors-like cells) and T (primitive streak-like cells) expression in gastruloids and neuruloids with different starting ratio of ES and XEN cells (z-projection of whole mount immunostaining). Scale bars 100 µm. b , SOX2 and OCT4 expression (immunostaining) in sections of neuruloids at 96 h (left, scale bars 100 µm) and cultured ESCs (right, scale bars 10 µm). a-b, Cells nuclei were stained with DAPI.

    Article Snippet: Subsequently, slides were incubated overnight at 4 °C with the following primary antibodies diluted in blocking buffer: rat anti-SOX2 (1:200, 14-9811-82, Thermo Fisher Scientific), goat anti-T (1:200, sc-17745, Santa Cruz Biotechnology), mouse anti-N-cadherin (1:200, 33-3900, Thermo Fisher Scientific), rabbit anti-E-cadherin (1:200, 3195, Cell Signaling Technology), rabbit anti-PAX6 (1:100 (cerebral organoids) or 1:200 (gastruloids, neuruloids), 42-6600, Thermo Fisher Scientific), mouse anti-NKX6.1 (1:200, F55A12, Developmental Studies Hybridoma Bank), rabbit anti-NKX6.1 (1:200, HPA036774, Merck), mouse anti-TUJ1 (1:200, 801202, BioLegend), rabbit anti-CD31 (1:50, ab28364, Abcam), rabbit anti-GATA6 (1:200, PA1-104, Thermo Fisher Scientific), goat anti-GATA6 (1:200, AF1700, R & D Systems), rabbit anti-Laminin (1:200, PA1-16730, Thermo Fisher Scientific), mouse anti-OCT4 (1:200, MA1-104, Thermo Fisher Scientific).

    Techniques: Expressing, Immunostaining, Cell Culture, Staining