MA1045 Search Results


  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 96
    Thermo Fisher psd 95
    Loss of Cys cluster I (GluN2B 3CS) palmitoylation of GluN2B does not affect the surface expression of 2B-NMDARs in striatal neurons. GFP-tagged GluN2B wild type (WT) and Cys cluster I mutant (GluN2B 3CS) were nucleofected in striatal neurons co-cultured with cortical neurons. (A) Images representing the surface/internal expression of GluN2B in striatal neurons in MSN-CTX co-cultures from FVB/N mice. Neuronal cultures were live stained for surface GluN2B (green) with GFP antibody at DIV 18, then fixed and stained for internal GluN2B (red). Merged image shows the total GluN2B expression. Scale bars, 20 μm. (B) Representative images of surface (green)/internal (red) expression of GluN2B in striatal neurons co-cultured with cortical neurons from YAC128 mice. (C,D) Images representing the colocalization of surface GluN2B (green) with excitatory synaptic markers, <t>PSD-95</t> (red) and vGLUT1 (blue), in striatal neurons in MSN-CTX co-cultures expressing the wild type and Cys cluster I mutant (GluN2B 3CS). Scale bars, 10 μm. (E) Quantitative analysis for the ratio of surface/internal GluN2B intensity. Data from FVB/N and YAC128 co-cultures were acquired in paired experiments ( N = 4 paired culture batches, 52 cells for each genotype and construct). GluN2B WT surface intensity was significantly enhanced in YAC128 vs. FVB/N striatal neurons (two-way ANOVA, p = 0.0310 for mouse genotype, p = 0.7749 for GluN2B construct, and p = 0.0036 for interaction; ∗∗∗ p
    Psd 95, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/psd 95/product/Thermo Fisher
    Average 96 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    psd 95 - by Bioz Stars, 2021-07
    96/100 stars
      Buy from Supplier

    86
    Thermo Fisher psd95
    (A) Immunoblots of <t>PSD95</t> (cerebellum) of control (I) the experimental (IIa, b; IIIa, b; IVa, b) animals along with loading control (GAPDH). (B) Bar diagram showing fold change in PSD95 expression in the experimental (IIa, b; IIIa, b; IVa, b) vs control group. Values are Mean ± SD. Significant at p
    Psd95, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/psd95/product/Thermo Fisher
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    psd95 - by Bioz Stars, 2021-07
    86/100 stars
      Buy from Supplier

    86
    Thermo Fisher anti psd 95
    Expression of pre- and postsynaptic proteins in KIF3B overexpressed cortical neurons. (A,D) Representative confocal images of cortical neurons 48 h after transfection with eGFP and FLKIF3B. (B,C,E,F) The bar graph depicts the total number of <t>PSD-95</t> and VGLUT1 punta per μm of GFP. The analysis was performed using ImageJ (NIH). Student t -test, ns P > 0.05. Scale bar, 2 μm, N (number of neurons) is indicated per group. Error bars are SEM. Data used for preparing plots are shown in Supplementary Table 7 .
    Anti Psd 95, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/anti psd 95/product/Thermo Fisher
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    anti psd 95 - by Bioz Stars, 2021-07
    86/100 stars
      Buy from Supplier

    Image Search Results


    Loss of Cys cluster I (GluN2B 3CS) palmitoylation of GluN2B does not affect the surface expression of 2B-NMDARs in striatal neurons. GFP-tagged GluN2B wild type (WT) and Cys cluster I mutant (GluN2B 3CS) were nucleofected in striatal neurons co-cultured with cortical neurons. (A) Images representing the surface/internal expression of GluN2B in striatal neurons in MSN-CTX co-cultures from FVB/N mice. Neuronal cultures were live stained for surface GluN2B (green) with GFP antibody at DIV 18, then fixed and stained for internal GluN2B (red). Merged image shows the total GluN2B expression. Scale bars, 20 μm. (B) Representative images of surface (green)/internal (red) expression of GluN2B in striatal neurons co-cultured with cortical neurons from YAC128 mice. (C,D) Images representing the colocalization of surface GluN2B (green) with excitatory synaptic markers, PSD-95 (red) and vGLUT1 (blue), in striatal neurons in MSN-CTX co-cultures expressing the wild type and Cys cluster I mutant (GluN2B 3CS). Scale bars, 10 μm. (E) Quantitative analysis for the ratio of surface/internal GluN2B intensity. Data from FVB/N and YAC128 co-cultures were acquired in paired experiments ( N = 4 paired culture batches, 52 cells for each genotype and construct). GluN2B WT surface intensity was significantly enhanced in YAC128 vs. FVB/N striatal neurons (two-way ANOVA, p = 0.0310 for mouse genotype, p = 0.7749 for GluN2B construct, and p = 0.0036 for interaction; ∗∗∗ p

    Journal: Frontiers in Synaptic Neuroscience

    Article Title: Altered Regulation of Striatal Neuronal N-Methyl-D-Aspartate Receptor Trafficking by Palmitoylation in Huntington Disease Mouse Model

    doi: 10.3389/fnsyn.2019.00003

    Figure Lengend Snippet: Loss of Cys cluster I (GluN2B 3CS) palmitoylation of GluN2B does not affect the surface expression of 2B-NMDARs in striatal neurons. GFP-tagged GluN2B wild type (WT) and Cys cluster I mutant (GluN2B 3CS) were nucleofected in striatal neurons co-cultured with cortical neurons. (A) Images representing the surface/internal expression of GluN2B in striatal neurons in MSN-CTX co-cultures from FVB/N mice. Neuronal cultures were live stained for surface GluN2B (green) with GFP antibody at DIV 18, then fixed and stained for internal GluN2B (red). Merged image shows the total GluN2B expression. Scale bars, 20 μm. (B) Representative images of surface (green)/internal (red) expression of GluN2B in striatal neurons co-cultured with cortical neurons from YAC128 mice. (C,D) Images representing the colocalization of surface GluN2B (green) with excitatory synaptic markers, PSD-95 (red) and vGLUT1 (blue), in striatal neurons in MSN-CTX co-cultures expressing the wild type and Cys cluster I mutant (GluN2B 3CS). Scale bars, 10 μm. (E) Quantitative analysis for the ratio of surface/internal GluN2B intensity. Data from FVB/N and YAC128 co-cultures were acquired in paired experiments ( N = 4 paired culture batches, 52 cells for each genotype and construct). GluN2B WT surface intensity was significantly enhanced in YAC128 vs. FVB/N striatal neurons (two-way ANOVA, p = 0.0310 for mouse genotype, p = 0.7749 for GluN2B construct, and p = 0.0036 for interaction; ∗∗∗ p

    Article Snippet: For labeling of PSD-95 and VGLUT1, following incubation of live cells with anti-GFP antibodies to detect surface GluN2B as described above, cells were subsequently permeabilized with methanol for 5 min at -20°C, rinsed 3 times with PBST, then incubated with primary antibodies for PSD-95 (Thermo Scientific; 1:1000) and VGLUT1 (Millipore; 1:2000) at 4°C overnight, washed 3 times with PBST, and incubated with secondary antibodies conjugated to Alexa 568 fluorophore and AMCA (1:100) for 1 h at RT.

    Techniques: Expressing, Mutagenesis, Cell Culture, Mouse Assay, Staining, Construct

    Loss of Cys cluster II (GluN2B 5CS) palmitoylation of GluN2B regulates the surface expression of GluN2B in striatal neurons. GFP-tagged GluN2B wild type (WT) and Cys cluster II mutant (GluN2B 5CS) were nucleofected in striatal neurons co-cultured with cortical neurons. (A) Images representing the surface (green)/internal (red) expression of GluN2B in striatal neurons in MSN-CTX co-cultures from FVB/N mice. Merged image shows the total GluN2B expression. Scale bars, 20 μm. (B) Representative images of surface (green)/internal (red) expression of GluN2B in striatal neurons co-cultured with cortical neurons from YAC128 mice. (C,D) Representative images of surface GluN2B puncta colocalization with the excitatory synaptic markers PSD-95 and vGLUT1 for GluN2B WT and 5CS in FVB/N and YAC128 DIV 18 striatal neurons in MSN-CTX co-cultures. Scale bars, 10 μm. (E) Quantitative analysis for the ratio of surface to internal GluN2B intensity was performed for data from paired experiments from 4 batches each of FVB/N and YAC128 MSN-CTX co-cultures. Similar to results shown in Figure 3E , GluN2B WT surface intensity was significantly enhanced in YAC128 vs. FVB/N striatal neurons; as well, surface intensity was significantly enhanced in FVB/N but not YAC128 striatal neurons expressing the GluN2B 5CS [FVB/N: N = 4(48 cells), YAC128: N = 4(42 cells)] when compared to neurons expressing GluN2B WT [FVB/N: N = 4(48 cells), YAC128: N = 4(42 cells)]. Significant by two-way ANOVA, p = 0.0012 for genotype, p = 0.0129 for 2B construct, and p = 0.9970 for interaction; ∗∗ p

    Journal: Frontiers in Synaptic Neuroscience

    Article Title: Altered Regulation of Striatal Neuronal N-Methyl-D-Aspartate Receptor Trafficking by Palmitoylation in Huntington Disease Mouse Model

    doi: 10.3389/fnsyn.2019.00003

    Figure Lengend Snippet: Loss of Cys cluster II (GluN2B 5CS) palmitoylation of GluN2B regulates the surface expression of GluN2B in striatal neurons. GFP-tagged GluN2B wild type (WT) and Cys cluster II mutant (GluN2B 5CS) were nucleofected in striatal neurons co-cultured with cortical neurons. (A) Images representing the surface (green)/internal (red) expression of GluN2B in striatal neurons in MSN-CTX co-cultures from FVB/N mice. Merged image shows the total GluN2B expression. Scale bars, 20 μm. (B) Representative images of surface (green)/internal (red) expression of GluN2B in striatal neurons co-cultured with cortical neurons from YAC128 mice. (C,D) Representative images of surface GluN2B puncta colocalization with the excitatory synaptic markers PSD-95 and vGLUT1 for GluN2B WT and 5CS in FVB/N and YAC128 DIV 18 striatal neurons in MSN-CTX co-cultures. Scale bars, 10 μm. (E) Quantitative analysis for the ratio of surface to internal GluN2B intensity was performed for data from paired experiments from 4 batches each of FVB/N and YAC128 MSN-CTX co-cultures. Similar to results shown in Figure 3E , GluN2B WT surface intensity was significantly enhanced in YAC128 vs. FVB/N striatal neurons; as well, surface intensity was significantly enhanced in FVB/N but not YAC128 striatal neurons expressing the GluN2B 5CS [FVB/N: N = 4(48 cells), YAC128: N = 4(42 cells)] when compared to neurons expressing GluN2B WT [FVB/N: N = 4(48 cells), YAC128: N = 4(42 cells)]. Significant by two-way ANOVA, p = 0.0012 for genotype, p = 0.0129 for 2B construct, and p = 0.9970 for interaction; ∗∗ p

    Article Snippet: For labeling of PSD-95 and VGLUT1, following incubation of live cells with anti-GFP antibodies to detect surface GluN2B as described above, cells were subsequently permeabilized with methanol for 5 min at -20°C, rinsed 3 times with PBST, then incubated with primary antibodies for PSD-95 (Thermo Scientific; 1:1000) and VGLUT1 (Millipore; 1:2000) at 4°C overnight, washed 3 times with PBST, and incubated with secondary antibodies conjugated to Alexa 568 fluorophore and AMCA (1:100) for 1 h at RT.

    Techniques: Expressing, Mutagenesis, Cell Culture, Mouse Assay, Construct

    (A) Immunoblots of PSD95 (cerebellum) of control (I) the experimental (IIa, b; IIIa, b; IVa, b) animals along with loading control (GAPDH). (B) Bar diagram showing fold change in PSD95 expression in the experimental (IIa, b; IIIa, b; IVa, b) vs control group. Values are Mean ± SD. Significant at p

    Journal: Journal of Ayurveda and Integrative Medicine

    Article Title: Ameliorative role of antioxidant supplementation on sodium-arsenite induced adverse effects on the developing rat cerebellum

    doi: 10.1016/j.jaim.2018.02.138

    Figure Lengend Snippet: (A) Immunoblots of PSD95 (cerebellum) of control (I) the experimental (IIa, b; IIIa, b; IVa, b) animals along with loading control (GAPDH). (B) Bar diagram showing fold change in PSD95 expression in the experimental (IIa, b; IIIa, b; IVa, b) vs control group. Values are Mean ± SD. Significant at p

    Article Snippet: Specific primary antibodies- Syp (Santa Cruz Biotechnology. sc9116); PSD95 (Pierce Biotechnology, Inc. MA1045) and loading control (GAPDH & β actin; Bioss, USA) in dilutions of 1:1000 were used for primary incubation followed by overnight incubation with secondary antibody (Goat anti mouse sc 2005 or Goat anti rabbit sc 2004, SantaCruz Biotechnology; dilution- 1:2000).

    Techniques: Western Blot, Expressing

    Immuno-histochemical localization of PSD95 (→) in ML, GCL areas intervening between PCs of cerebellar cortex from control (A) experimental (B, C, D, E, F, G) groups (40X). Note: Decrease in the cerebellar PSD95 immunoreactivity in B (IIa) C (IIb) as compared to group A (I), D (IIIa), E (IIIb), F (IVa) G (IVb).

    Journal: Journal of Ayurveda and Integrative Medicine

    Article Title: Ameliorative role of antioxidant supplementation on sodium-arsenite induced adverse effects on the developing rat cerebellum

    doi: 10.1016/j.jaim.2018.02.138

    Figure Lengend Snippet: Immuno-histochemical localization of PSD95 (→) in ML, GCL areas intervening between PCs of cerebellar cortex from control (A) experimental (B, C, D, E, F, G) groups (40X). Note: Decrease in the cerebellar PSD95 immunoreactivity in B (IIa) C (IIb) as compared to group A (I), D (IIIa), E (IIIb), F (IVa) G (IVb).

    Article Snippet: Specific primary antibodies- Syp (Santa Cruz Biotechnology. sc9116); PSD95 (Pierce Biotechnology, Inc. MA1045) and loading control (GAPDH & β actin; Bioss, USA) in dilutions of 1:1000 were used for primary incubation followed by overnight incubation with secondary antibody (Goat anti mouse sc 2005 or Goat anti rabbit sc 2004, SantaCruz Biotechnology; dilution- 1:2000).

    Techniques:

    Expression of pre- and postsynaptic proteins in KIF3B overexpressed cortical neurons. (A,D) Representative confocal images of cortical neurons 48 h after transfection with eGFP and FLKIF3B. (B,C,E,F) The bar graph depicts the total number of PSD-95 and VGLUT1 punta per μm of GFP. The analysis was performed using ImageJ (NIH). Student t -test, ns P > 0.05. Scale bar, 2 μm, N (number of neurons) is indicated per group. Error bars are SEM. Data used for preparing plots are shown in Supplementary Table 7 .

    Journal: Frontiers in Cellular Neuroscience

    Article Title: Molecular Motor KIF3B Acts as a Key Regulator of Dendritic Architecture in Cortical Neurons

    doi: 10.3389/fncel.2020.521199

    Figure Lengend Snippet: Expression of pre- and postsynaptic proteins in KIF3B overexpressed cortical neurons. (A,D) Representative confocal images of cortical neurons 48 h after transfection with eGFP and FLKIF3B. (B,C,E,F) The bar graph depicts the total number of PSD-95 and VGLUT1 punta per μm of GFP. The analysis was performed using ImageJ (NIH). Student t -test, ns P > 0.05. Scale bar, 2 μm, N (number of neurons) is indicated per group. Error bars are SEM. Data used for preparing plots are shown in Supplementary Table 7 .

    Article Snippet: The cells were incubated in 10% normal horse serum in D-PBS with 0.1% Triton X-100 for 1 h to prevent non-specific binding of the primary antibody followed by overnight incubation at 4°C with the primary antibodies: anti-Synaptophysin (1:1,000, ab32594, Abcam), anti-Piccolo (1:1,000, 142104, Synaptic Systems), anti-VGLUT1 (1:1,000, 135-304, Synaptic Systems), anti-PSD-95 (1:1,000, MA1-045, Thermo Fisher Scientific) and anti-GFP (1:4,000, AF4240, Novus Biologicals) in the blocking solution.

    Techniques: Expressing, Transfection

    Expression of pre- and postsynaptic proteins in KIF3B knockdown cortical neurons. (A,D) Representative confocal images of cortical neurons 72 h after transfection with shScrambled and shKIF3B. (B,C,E,F) The bar graph depicts the corrected total cell fluorescence (CTCF) of Synaptophysin, Piccolo, PSD-95, and VGLUT1 analyzed using ImageJ (NIH). Student t -test, * P

    Journal: Frontiers in Cellular Neuroscience

    Article Title: Molecular Motor KIF3B Acts as a Key Regulator of Dendritic Architecture in Cortical Neurons

    doi: 10.3389/fncel.2020.521199

    Figure Lengend Snippet: Expression of pre- and postsynaptic proteins in KIF3B knockdown cortical neurons. (A,D) Representative confocal images of cortical neurons 72 h after transfection with shScrambled and shKIF3B. (B,C,E,F) The bar graph depicts the corrected total cell fluorescence (CTCF) of Synaptophysin, Piccolo, PSD-95, and VGLUT1 analyzed using ImageJ (NIH). Student t -test, * P

    Article Snippet: The cells were incubated in 10% normal horse serum in D-PBS with 0.1% Triton X-100 for 1 h to prevent non-specific binding of the primary antibody followed by overnight incubation at 4°C with the primary antibodies: anti-Synaptophysin (1:1,000, ab32594, Abcam), anti-Piccolo (1:1,000, 142104, Synaptic Systems), anti-VGLUT1 (1:1,000, 135-304, Synaptic Systems), anti-PSD-95 (1:1,000, MA1-045, Thermo Fisher Scientific) and anti-GFP (1:4,000, AF4240, Novus Biologicals) in the blocking solution.

    Techniques: Expressing, Transfection, Fluorescence