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  • 99
    New England Biolabs full length
    Single factor experiment of RealAmp. The influence of each variable on the LAMP reaction was analyzed by the amplification curve ( a ) and the melt peak ( b ). For the <t>Bst</t> <t>DNA</t> polymerase optimization, Bst polymerase quantities were adjusted to 2 U, 4 U, 6 U and 8 U, respectively. Ratios of inner to outer primers were set at 2:1, 4:1, 6:1 and 8:1 with the outer primer concentration fixed to 0.2 μM to optimize the primer ratios. Mg 2+ concentrations in the LAMP reactions were varied from 5 mM, to 8 mM for the optimization of Mg 2+ .
    Full Length, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 99/100, based on 243 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Single factor experiment of RealAmp. The influence of each variable on the LAMP reaction was analyzed by the amplification curve ( a ) and the melt peak ( b ). For the Bst DNA polymerase optimization, Bst polymerase quantities were adjusted to 2 U, 4 U, 6 U and 8 U, respectively. Ratios of inner to outer primers were set at 2:1, 4:1, 6:1 and 8:1 with the outer primer concentration fixed to 0.2 μM to optimize the primer ratios. Mg 2+ concentrations in the LAMP reactions were varied from 5 mM, to 8 mM for the optimization of Mg 2+ .

    Journal: Scientific Reports

    Article Title: Development of a real-time fluorescence loop-mediated isothermal amplification assay for rapid and quantitative detection of Ustilago maydis

    doi: 10.1038/s41598-017-13881-4

    Figure Lengend Snippet: Single factor experiment of RealAmp. The influence of each variable on the LAMP reaction was analyzed by the amplification curve ( a ) and the melt peak ( b ). For the Bst DNA polymerase optimization, Bst polymerase quantities were adjusted to 2 U, 4 U, 6 U and 8 U, respectively. Ratios of inner to outer primers were set at 2:1, 4:1, 6:1 and 8:1 with the outer primer concentration fixed to 0.2 μM to optimize the primer ratios. Mg 2+ concentrations in the LAMP reactions were varied from 5 mM, to 8 mM for the optimization of Mg 2+ .

    Article Snippet: In this work, we showed that as the concentration of Bst DNA polymerase was increased from 4 U to 8 U, the amplification improved dramatically, however, the tendency of amplification efficiency improvement from 6 U to 8 U is far less than that of 4 U to 6 U (Figs and ).

    Techniques: Amplification, Concentration Assay