Journal: Scientific Reports
Article Title: Development of a real-time fluorescence loop-mediated isothermal amplification assay for rapid and quantitative detection of Ustilago maydis
Figure Lengend Snippet: Single factor experiment of RealAmp. The influence of each variable on the LAMP reaction was analyzed by the amplification curve ( a ) and the melt peak ( b ). For the Bst DNA polymerase optimization, Bst polymerase quantities were adjusted to 2 U, 4 U, 6 U and 8 U, respectively. Ratios of inner to outer primers were set at 2:1, 4:1, 6:1 and 8:1 with the outer primer concentration fixed to 0.2 μM to optimize the primer ratios. Mg 2+ concentrations in the LAMP reactions were varied from 5 mM, to 8 mM for the optimization of Mg 2+ .
Article Snippet: In this work, we showed that as the concentration of Bst DNA polymerase was increased from 4 U to 8 U, the amplification improved dramatically, however, the tendency of amplification efficiency improvement from 6 U to 8 U is far less than that of 4 U to 6 U (Figs and ).
Techniques: Amplification, Concentration Assay