Journal: Journal of Bacteriology
Article Title: Interaction of Apurinic/Apyrimidinic Endonucleases Nfo and ExoA with the DNA Integrity Scanning Protein DisA in the Processing of Oxidative DNA Damage during Bacillus subtilis Spore Outgrowth
Figure Lengend Snippet: AGE analysis of DNA isolated from dormant or outgrown spores of different strains and with or without treatment with EndoIV or Fpg. Chromosomal DNAs from dormant spores (DS) or outgrown spores (OG) of the wild-type (A), nfo exoA (B), or nfo exoA disA
Article Snippet: Samples of chromosomal DNA isolated from germinated or dormant spores of each strain were incubated with 18 units of endonuclease IV (EndoIV), which cleaves DNA at apurinic/apyrimidinic (AP) sites, or 14 units of formamidopyrimidine-DNA glycosylase (Fpg), which cleaves DNA at 8-oxo-guanine (8-oxo-G) residues, respectively, according to the instructions of the supplier (New England BioLabs).