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    New England Biolabs endonuclease iv
    AGE analysis of <t>DNA</t> isolated from dormant or outgrown spores of different strains and with or without treatment with <t>EndoIV</t> or Fpg. Chromosomal DNAs from dormant spores (DS) or outgrown spores (OG) of the wild-type (A), nfo exoA (B), or nfo exoA disA
    Endonuclease Iv, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 95/100, based on 68 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    endonuclease iv - by Bioz Stars, 2020-09
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    AGE analysis of DNA isolated from dormant or outgrown spores of different strains and with or without treatment with EndoIV or Fpg. Chromosomal DNAs from dormant spores (DS) or outgrown spores (OG) of the wild-type (A), nfo exoA (B), or nfo exoA disA

    Journal: Journal of Bacteriology

    Article Title: Interaction of Apurinic/Apyrimidinic Endonucleases Nfo and ExoA with the DNA Integrity Scanning Protein DisA in the Processing of Oxidative DNA Damage during Bacillus subtilis Spore Outgrowth

    doi: 10.1128/JB.01259-13

    Figure Lengend Snippet: AGE analysis of DNA isolated from dormant or outgrown spores of different strains and with or without treatment with EndoIV or Fpg. Chromosomal DNAs from dormant spores (DS) or outgrown spores (OG) of the wild-type (A), nfo exoA (B), or nfo exoA disA

    Article Snippet: Samples of chromosomal DNA isolated from germinated or dormant spores of each strain were incubated with 18 units of endonuclease IV (EndoIV), which cleaves DNA at apurinic/apyrimidinic (AP) sites, or 14 units of formamidopyrimidine-DNA glycosylase (Fpg), which cleaves DNA at 8-oxo-guanine (8-oxo-G) residues, respectively, according to the instructions of the supplier (New England BioLabs).

    Techniques: Isolation

    Ratios of the chemical yields of prompt SSBs and of Nth-, Fpg- and Nfo-sensitive sites obtained in the presence of edaravone at several concentrations ( G ) to the yields obtained without edaravone ( G 0 ).

    Journal: Journal of Radiation Research

    Article Title: Chemical repair activity of free radical scavenger edaravone: reduction reactions with dGMP hydroxyl radical adducts and suppression of base lesions and AP sites on irradiated plasmid DNA

    doi: 10.1093/jrr/rru079

    Figure Lengend Snippet: Ratios of the chemical yields of prompt SSBs and of Nth-, Fpg- and Nfo-sensitive sites obtained in the presence of edaravone at several concentrations ( G ) to the yields obtained without edaravone ( G 0 ).

    Article Snippet: Formamidopyrimidine-DNA glycosylase (Fpg), endonuclease III (Nth), endonuclease IV (Nfo), and reaction buffer solutions used for the treatment of these enzymes were purchased from New England BioLabs Inc. (Ipswich, MA).

    Techniques: