Journal: The Journal of Biological Chemistry
Article Title: Polynucleotide 3′-terminal Phosphate Modifications by RNA and DNA Ligases
Figure Lengend Snippet: RNA 3 ′ -terminal phosphate modifications with MthRnl. A , gel shift analysis of archaeal RNA ligase (MthRnl) reaction products of either FAM-RNA17p ( column I ) or FAM-RNA17(OMe)p ( column II ) followed by phosphatase treatment with either AnP or PNK.
Article Snippet: Antarctic phosphatase (AnP), T4 PNK, and yeast 5′Deadenylase were from New England Biolabs.
Techniques: Electrophoretic Mobility Shift Assay, Aqueous Normal-phase Chromatography