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  • 96
    New England Biolabs bst
    Schematic of RCA of circularized padlock probes . (A) S. brasiliensis (Sbra-RCA) padlock probe design. (B) CAL is amplified by PCR with primers CAL -Fw and CAL -Rv; PCR products are submitted to ligation. Circularization of padlock probes occurs only if both probe arms hybridize correctly to the target sequence. (C) Upon specific hybridization, the phosphorylated 5′ end and the free hydroxyl at the 3′ end of the probe are joined by Pfu <t>DNA</t> ligase. After ligation, non-circularized probes and single-stranded primers are removed with Exo I and Exo III (optional step). (D) Circularized padlock probes serve as DNA template and signal amplification occurs via RCA, using <t>Bst</t> DNA polymerase and the primers RCA1 and RCA2. DNA synthesis occurs continuously for 1 h under isothermal amplification (65°C). DNA products are detected by gel electrophoresis or directly with SYBR Green I.
    Bst, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 96/100, based on 350 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/bst/product/New England Biolabs
    Average 96 stars, based on 350 article reviews
    Price from $9.99 to $1999.99
    bst - by Bioz Stars, 2020-08
    96/100 stars
      Buy from Supplier

    99
    Millipore m7278
    Downregulation of Fau inhibits UV-induced apoptosis of <t>T-47D</t> breast cancer cells. T-47D cells were transfected with one of two different siRNAs to Fau or with a negative control (NC) (Ambion code 4611) siRNA using RNAiFect (Qiagen). After 120 hours samples were collected for real-time RT-PCR analysis, and trypsinized cells were either exposed to UV light (40 J/m 2 ; closed bars) or were mock-irradiated (open bars), and then replated in fresh <t>medium.</t> (a) Real-time RT-PCR analysis of Fau transcript levels. Data, expressed relative to the house-keeping gene ALAS1 , are the mean ± standard error of the mean. * P
    M7278, supplied by Millipore, used in various techniques. Bioz Stars score: 99/100, based on 1926 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/m7278/product/Millipore
    Average 99 stars, based on 1926 article reviews
    Price from $9.99 to $1999.99
    m7278 - by Bioz Stars, 2020-08
    99/100 stars
      Buy from Supplier

    Image Search Results


    Schematic of RCA of circularized padlock probes . (A) S. brasiliensis (Sbra-RCA) padlock probe design. (B) CAL is amplified by PCR with primers CAL -Fw and CAL -Rv; PCR products are submitted to ligation. Circularization of padlock probes occurs only if both probe arms hybridize correctly to the target sequence. (C) Upon specific hybridization, the phosphorylated 5′ end and the free hydroxyl at the 3′ end of the probe are joined by Pfu DNA ligase. After ligation, non-circularized probes and single-stranded primers are removed with Exo I and Exo III (optional step). (D) Circularized padlock probes serve as DNA template and signal amplification occurs via RCA, using Bst DNA polymerase and the primers RCA1 and RCA2. DNA synthesis occurs continuously for 1 h under isothermal amplification (65°C). DNA products are detected by gel electrophoresis or directly with SYBR Green I.

    Journal: Frontiers in Microbiology

    Article Title: Rapid Identification of Emerging Human-Pathogenic Sporothrix Species with Rolling Circle Amplification

    doi: 10.3389/fmicb.2015.01385

    Figure Lengend Snippet: Schematic of RCA of circularized padlock probes . (A) S. brasiliensis (Sbra-RCA) padlock probe design. (B) CAL is amplified by PCR with primers CAL -Fw and CAL -Rv; PCR products are submitted to ligation. Circularization of padlock probes occurs only if both probe arms hybridize correctly to the target sequence. (C) Upon specific hybridization, the phosphorylated 5′ end and the free hydroxyl at the 3′ end of the probe are joined by Pfu DNA ligase. After ligation, non-circularized probes and single-stranded primers are removed with Exo I and Exo III (optional step). (D) Circularized padlock probes serve as DNA template and signal amplification occurs via RCA, using Bst DNA polymerase and the primers RCA1 and RCA2. DNA synthesis occurs continuously for 1 h under isothermal amplification (65°C). DNA products are detected by gel electrophoresis or directly with SYBR Green I.

    Article Snippet: Circularized probes were amplified with replication primers RCA1 and RCA2 with Bst DNA polymerase.

    Techniques: Amplification, Polymerase Chain Reaction, Ligation, Sequencing, Hybridization, DNA Synthesis, Nucleic Acid Electrophoresis, SYBR Green Assay

    Downregulation of Fau inhibits UV-induced apoptosis of T-47D breast cancer cells. T-47D cells were transfected with one of two different siRNAs to Fau or with a negative control (NC) (Ambion code 4611) siRNA using RNAiFect (Qiagen). After 120 hours samples were collected for real-time RT-PCR analysis, and trypsinized cells were either exposed to UV light (40 J/m 2 ; closed bars) or were mock-irradiated (open bars), and then replated in fresh medium. (a) Real-time RT-PCR analysis of Fau transcript levels. Data, expressed relative to the house-keeping gene ALAS1 , are the mean ± standard error of the mean. * P

    Journal: Breast Cancer Research : BCR

    Article Title: Dysregulated expression of Fau and MELK is associated with poor prognosis in breast cancer

    doi: 10.1186/bcr2350

    Figure Lengend Snippet: Downregulation of Fau inhibits UV-induced apoptosis of T-47D breast cancer cells. T-47D cells were transfected with one of two different siRNAs to Fau or with a negative control (NC) (Ambion code 4611) siRNA using RNAiFect (Qiagen). After 120 hours samples were collected for real-time RT-PCR analysis, and trypsinized cells were either exposed to UV light (40 J/m 2 ; closed bars) or were mock-irradiated (open bars), and then replated in fresh medium. (a) Real-time RT-PCR analysis of Fau transcript levels. Data, expressed relative to the house-keeping gene ALAS1 , are the mean ± standard error of the mean. * P

    Article Snippet: Breast cancer cell culture The human breast cancer cell line T-47D was maintained in MEM (M5650; Sigma, Gillingham, Dorset, UK) supplemented with 5% heat-inactivated FCS, 2 mM L -glutamine, 10 μg/ml insulin and 50 μg/ml gentamycin.

    Techniques: Transfection, Negative Control, Quantitative RT-PCR, Irradiation