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  • 88
    Boster Bio rabbit anti k14
    Rabbit Anti K14, supplied by Boster Bio, used in various techniques. Bioz Stars score: 88/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/rabbit anti k14/product/Boster Bio
    Average 88 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    rabbit anti k14 - by Bioz Stars, 2023-01
    88/100 stars
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    86
    New England Biolabs q5 high fidelity polymerase
    Q5 High Fidelity Polymerase, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/q5 high fidelity polymerase/product/New England Biolabs
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    q5 high fidelity polymerase - by Bioz Stars, 2023-01
    86/100 stars
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    86
    Genecopoeia flag wt stk25 vector
    A) Differential phosphoproteomic spectra of <t>STK25</t> +/+ and STK25 -/- cardiomyocytes. Members of the PKA signaling pathway are highlighted. B) Ingenuity phosphoprotein pathway analysis. Orange indicates pathways upregulated in STK25 -/- cardiomyocytes while blue indicates upregulation in STK25 +/+ . C) PKA activity in response to 10μM forskolin in STK25 +/+ and STK25 -/- cardiomyocytes. n=3 for each condition. Mean +/-SD, *p=0.04, **p<0.01, ****p<0.0001 using ANOVA and Tukey’s adjustment for multiple comparisons. D) PKA activity in response to 10μM forskolin in HEK293T cells overexpressing either empty vector, wild type STK25 or kinase-dead K49R/T174A. n=3 for each condition. Mean +/-SD, *p=0.011, **p<0.01, ****p<0.0001 using ANOVA and Tukey’s adjustment for multiple comparisons.
    Flag Wt Stk25 Vector, supplied by Genecopoeia, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/flag wt stk25 vector/product/Genecopoeia
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    flag wt stk25 vector - by Bioz Stars, 2023-01
    86/100 stars
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    97
    Olympus dp22
    A) Differential phosphoproteomic spectra of <t>STK25</t> +/+ and STK25 -/- cardiomyocytes. Members of the PKA signaling pathway are highlighted. B) Ingenuity phosphoprotein pathway analysis. Orange indicates pathways upregulated in STK25 -/- cardiomyocytes while blue indicates upregulation in STK25 +/+ . C) PKA activity in response to 10μM forskolin in STK25 +/+ and STK25 -/- cardiomyocytes. n=3 for each condition. Mean +/-SD, *p=0.04, **p<0.01, ****p<0.0001 using ANOVA and Tukey’s adjustment for multiple comparisons. D) PKA activity in response to 10μM forskolin in HEK293T cells overexpressing either empty vector, wild type STK25 or kinase-dead K49R/T174A. n=3 for each condition. Mean +/-SD, *p=0.011, **p<0.01, ****p<0.0001 using ANOVA and Tukey’s adjustment for multiple comparisons.
    Dp22, supplied by Olympus, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/dp22/product/Olympus
    Average 97 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    dp22 - by Bioz Stars, 2023-01
    97/100 stars
      Buy from Supplier

    Image Search Results


    A) Differential phosphoproteomic spectra of STK25 +/+ and STK25 -/- cardiomyocytes. Members of the PKA signaling pathway are highlighted. B) Ingenuity phosphoprotein pathway analysis. Orange indicates pathways upregulated in STK25 -/- cardiomyocytes while blue indicates upregulation in STK25 +/+ . C) PKA activity in response to 10μM forskolin in STK25 +/+ and STK25 -/- cardiomyocytes. n=3 for each condition. Mean +/-SD, *p=0.04, **p<0.01, ****p<0.0001 using ANOVA and Tukey’s adjustment for multiple comparisons. D) PKA activity in response to 10μM forskolin in HEK293T cells overexpressing either empty vector, wild type STK25 or kinase-dead K49R/T174A. n=3 for each condition. Mean +/-SD, *p=0.011, **p<0.01, ****p<0.0001 using ANOVA and Tukey’s adjustment for multiple comparisons.

    Journal: bioRxiv

    Article Title: STK25 inhibits PKA signaling by phosphorylating PRKAR1A

    doi: 10.1101/2022.01.27.478084

    Figure Lengend Snippet: A) Differential phosphoproteomic spectra of STK25 +/+ and STK25 -/- cardiomyocytes. Members of the PKA signaling pathway are highlighted. B) Ingenuity phosphoprotein pathway analysis. Orange indicates pathways upregulated in STK25 -/- cardiomyocytes while blue indicates upregulation in STK25 +/+ . C) PKA activity in response to 10μM forskolin in STK25 +/+ and STK25 -/- cardiomyocytes. n=3 for each condition. Mean +/-SD, *p=0.04, **p<0.01, ****p<0.0001 using ANOVA and Tukey’s adjustment for multiple comparisons. D) PKA activity in response to 10μM forskolin in HEK293T cells overexpressing either empty vector, wild type STK25 or kinase-dead K49R/T174A. n=3 for each condition. Mean +/-SD, *p=0.011, **p<0.01, ****p<0.0001 using ANOVA and Tukey’s adjustment for multiple comparisons.

    Article Snippet: Vectors used include Flag-WT-STK25 vector (EX-M0142-M46, GeneCopoeia), Flag-K49R/T147A-STK25 vector (Vector Builder), Flag-empty vector (EX-NEG-M46, GeneCopoeia), V5-PRKAR1A (Vector Builder, VB200124-1141aes), V5-S77A/S83A-PRKAR1A (Vector Builder, VB200124-1126jtp), and V5-S77E/S83E-PRKAR1A (Vector Builder, VB200124-1127pst).

    Techniques: Activity Assay, Plasmid Preparation

    A) Immunoblot of PRKAR1A phosphorylation sites S77 and S83 in STK25 +/+ and STK25 -/- cardiomyocyte protein lysates. B) Immunoblots of phosphorylation sites S77 and S83 of PRKAR1A in STK25 -/- cardiomyocytes transfected with empty vector (EV), wild type STK25 and kinase dead K49R/T174A (loaded in duplicate). C) Forskolin (10μM) stimulated STK25 +/+ and STK25 -/- cardiomyocytes immunoblotted for phosphorylation of PRKAR1A and RYR2. D) Immunoprecipitation of Flag-STK25 expressed in HEK293T cells and immunoblotted for PRKAR1A and GM130 (positive control binding partner). E) Co-immunoprecipitation of PRKAR1A-V5 with STK25 and PRKACA in HEK293T cells treated with forskolin (10μM). F) In vitro kinase assay of purified STK25 and PRKAR1A, immunoblotted for phosphorylation of S83 of PRKAR1A.

    Journal: bioRxiv

    Article Title: STK25 inhibits PKA signaling by phosphorylating PRKAR1A

    doi: 10.1101/2022.01.27.478084

    Figure Lengend Snippet: A) Immunoblot of PRKAR1A phosphorylation sites S77 and S83 in STK25 +/+ and STK25 -/- cardiomyocyte protein lysates. B) Immunoblots of phosphorylation sites S77 and S83 of PRKAR1A in STK25 -/- cardiomyocytes transfected with empty vector (EV), wild type STK25 and kinase dead K49R/T174A (loaded in duplicate). C) Forskolin (10μM) stimulated STK25 +/+ and STK25 -/- cardiomyocytes immunoblotted for phosphorylation of PRKAR1A and RYR2. D) Immunoprecipitation of Flag-STK25 expressed in HEK293T cells and immunoblotted for PRKAR1A and GM130 (positive control binding partner). E) Co-immunoprecipitation of PRKAR1A-V5 with STK25 and PRKACA in HEK293T cells treated with forskolin (10μM). F) In vitro kinase assay of purified STK25 and PRKAR1A, immunoblotted for phosphorylation of S83 of PRKAR1A.

    Article Snippet: Vectors used include Flag-WT-STK25 vector (EX-M0142-M46, GeneCopoeia), Flag-K49R/T147A-STK25 vector (Vector Builder), Flag-empty vector (EX-NEG-M46, GeneCopoeia), V5-PRKAR1A (Vector Builder, VB200124-1141aes), V5-S77A/S83A-PRKAR1A (Vector Builder, VB200124-1126jtp), and V5-S77E/S83E-PRKAR1A (Vector Builder, VB200124-1127pst).

    Techniques: Western Blot, Transfection, Plasmid Preparation, Immunoprecipitation, Positive Control, Binding Assay, In Vitro, Kinase Assay, Purification

    A) Co-immunoprecipitations of V5 tagged PRKAR1A, S77A/S83A PRKAR1A or S77E/S83 PRKAR1A and immunoblotting for PRKARCA in HEK293T cells stimulated with forskolin (10μM). B) PKA activity in HEK293T cells stimulated with forskolin (10μM) and transfected with empty vector, wild type PRKAR1A, S77A/S83A PRKAR1A mutant or S77E/S83E PRKAR1A mutant as indicated. C) HEK293T cells transfected with the indicated vectors and assessed for growth by Real Time Glo for 5 hours after stimulation with forskolin (10μM). For all points in this figure, n=3 for each condition, Mean +/-SD, *p<0.05, ***p<0.001, and ****p<0.0001 by ANOVA with Tukey’s adjustment for multiple comparisons. D) PKA activity in HEK293T cells stimulated with forskolin (10μM) and either overexpressing STK25 and/or the siRNA of PRKAR1A. E) Model of STK25 downregulation of the PKA pathway through phosphorylation of PRKAR1A.

    Journal: bioRxiv

    Article Title: STK25 inhibits PKA signaling by phosphorylating PRKAR1A

    doi: 10.1101/2022.01.27.478084

    Figure Lengend Snippet: A) Co-immunoprecipitations of V5 tagged PRKAR1A, S77A/S83A PRKAR1A or S77E/S83 PRKAR1A and immunoblotting for PRKARCA in HEK293T cells stimulated with forskolin (10μM). B) PKA activity in HEK293T cells stimulated with forskolin (10μM) and transfected with empty vector, wild type PRKAR1A, S77A/S83A PRKAR1A mutant or S77E/S83E PRKAR1A mutant as indicated. C) HEK293T cells transfected with the indicated vectors and assessed for growth by Real Time Glo for 5 hours after stimulation with forskolin (10μM). For all points in this figure, n=3 for each condition, Mean +/-SD, *p<0.05, ***p<0.001, and ****p<0.0001 by ANOVA with Tukey’s adjustment for multiple comparisons. D) PKA activity in HEK293T cells stimulated with forskolin (10μM) and either overexpressing STK25 and/or the siRNA of PRKAR1A. E) Model of STK25 downregulation of the PKA pathway through phosphorylation of PRKAR1A.

    Article Snippet: Vectors used include Flag-WT-STK25 vector (EX-M0142-M46, GeneCopoeia), Flag-K49R/T147A-STK25 vector (Vector Builder), Flag-empty vector (EX-NEG-M46, GeneCopoeia), V5-PRKAR1A (Vector Builder, VB200124-1141aes), V5-S77A/S83A-PRKAR1A (Vector Builder, VB200124-1126jtp), and V5-S77E/S83E-PRKAR1A (Vector Builder, VB200124-1127pst).

    Techniques: Western Blot, Activity Assay, Transfection, Plasmid Preparation, Mutagenesis

    A) Immmunoblot of Stk25, Prkaca, Gapdh, phospho-S77, phospho-S83 and total Prkar1a in Stk25 +/+ and Stk25 -/ - whole heart lysates. B) Densitometry analysis phospho-S77 and S83 relative to total Prkar1a in immunoblot shown in B. C) Stk25 +/+ and Stk25 -/- mouse heart lysates were assessed for PKA activity in vitro , n=6 for each condition. D) Representative m-mode images of Stk25 +/+ and Stk25 -/- mouse hearts stimulated with either control or isoproterenol. E) Fold change in individual heart response to isoproterenol when compared to unstimulated for ejection fraction (EF), fractional shortening (FS), n=5 for Stk25 +/+ and n=6 for Stk25 -/- . F) RT-PCR (left) from left ventricular myocardium of normal hearts (n=6) or heart failure (n=17) expressed as a ratio of the threshold cycle curve (Ct) of STK25 to GAPDH . Immunoblot (right) of STK25 and PRKAR1A expression and phosphorylation in protein lysates from left ventricular myocardium of normal hearts or failing hearts. N=6 for both healthy control and heart failure. Mean +/-SD, *p<0.05, **p<0.01 by student t-test in 4A and 4C and Welch’s t-test in 4E and 4F.

    Journal: bioRxiv

    Article Title: STK25 inhibits PKA signaling by phosphorylating PRKAR1A

    doi: 10.1101/2022.01.27.478084

    Figure Lengend Snippet: A) Immmunoblot of Stk25, Prkaca, Gapdh, phospho-S77, phospho-S83 and total Prkar1a in Stk25 +/+ and Stk25 -/ - whole heart lysates. B) Densitometry analysis phospho-S77 and S83 relative to total Prkar1a in immunoblot shown in B. C) Stk25 +/+ and Stk25 -/- mouse heart lysates were assessed for PKA activity in vitro , n=6 for each condition. D) Representative m-mode images of Stk25 +/+ and Stk25 -/- mouse hearts stimulated with either control or isoproterenol. E) Fold change in individual heart response to isoproterenol when compared to unstimulated for ejection fraction (EF), fractional shortening (FS), n=5 for Stk25 +/+ and n=6 for Stk25 -/- . F) RT-PCR (left) from left ventricular myocardium of normal hearts (n=6) or heart failure (n=17) expressed as a ratio of the threshold cycle curve (Ct) of STK25 to GAPDH . Immunoblot (right) of STK25 and PRKAR1A expression and phosphorylation in protein lysates from left ventricular myocardium of normal hearts or failing hearts. N=6 for both healthy control and heart failure. Mean +/-SD, *p<0.05, **p<0.01 by student t-test in 4A and 4C and Welch’s t-test in 4E and 4F.

    Article Snippet: Vectors used include Flag-WT-STK25 vector (EX-M0142-M46, GeneCopoeia), Flag-K49R/T147A-STK25 vector (Vector Builder), Flag-empty vector (EX-NEG-M46, GeneCopoeia), V5-PRKAR1A (Vector Builder, VB200124-1141aes), V5-S77A/S83A-PRKAR1A (Vector Builder, VB200124-1126jtp), and V5-S77E/S83E-PRKAR1A (Vector Builder, VB200124-1127pst).

    Techniques: Western Blot, Activity Assay, In Vitro, Reverse Transcription Polymerase Chain Reaction, Expressing