Journal: Chinese Medicine

Article Title: In vitro and in vivo antitumor effects of acetylshikonin isolated from Arnebia euchroma (Royle) Johnst (Ruanzicao) cell suspension cultures

doi: 10.1186/1749-8546-4-14

Figure Lengend Snippet: Bax, bcl-2 and caspase-3 protein expression in LLC tissues (×400) . (A) Bax control; (B) Bax acetylshikonin (2 mg/kg); (C) Bcl-2 control; (D) Bcl-2 acetylshikonin (2 mg/kg); (E) caspase-3 control; (F) caspase-3 acetylshikonin (2 mg/kg).

Article Snippet: The primary antibodies, namely bcl-2, bax and caspase-3 (Wuhan Boster Biological Technology, China), were diluted at 1:100.

Techniques: Expressing

Journal: Frontiers in Microbiology

Article Title: Hepatitis E Virus Induces Hepatocyte Apoptosis via Mitochondrial Pathway in Mongolian Gerbils

doi: 10.3389/fmicb.2018.00460

Figure Lengend Snippet: Immunohistochemical and western-blot analysis of Apaf-1, active caspase-9, and active caspase-3 proteins. (A1) IHC analysis of livers in control group, (A2) IHC analysis of livers in inoculated group. The primary antibody was Apaf-1 antibody, and the positive signals were observed in the cytoplasm of hepatocyte (→); (A3) A semi-quantitative analysis of the ratio of Apaf-1 positive staining to the total field; (A4) Western-blot analysis of Apaf-1 protein in hepatocyte of control group and inoculated group gerbils; ( A5 ) A semi-quantitative analysis of relative expression of Apaf-1/β-actin. (B1) IHC analysis of livers in control group, (B2) IHC analysis of livers in inoculated group. The primary antibody was active caspase-9 antibody, and the positive signals were observed in the cytoplasm of hepatocyte (→); (B3) A semi-quantitative analysis of the ratio of active caspase-9 positive staining to the total field; (B4) Western-blot analysis of active caspase-9 protein in hepatocyte of control group and inoculated group gerbils; (B5) A semi-quantitative analysis of relative expression of active caspase-9/GAPDH. (C1) IHC analysis of livers in control group, (C2) IHC analysis of livers in inoculated group. The primary antibody was active caspase-3 antibody, and the positive signals were observed in the cytoplasm of hepatocyte (→); (C3) A semi-quantitative analysis of the ratio of active caspase-3 positive staining to the total field; (C4) Western-blot analysis of active caspase-3 protein in hepatocyte of control group and inoculated group gerbils; (C5) A semi-quantitative analysis of relative expression of active caspase-3/β-actin. ∗ p

Article Snippet: Primary antibodies used in this study were anti-Apaf-1 (1:200, BA2373), anti-Bax (1:300, BA0315), anti-Bcl-2 (1:200, BA0412) and anti-active caspase-3 (1:300, BA3968), anti-active caspase-9 (1:300, BA0690), all obtained from Boster, Co., Ltd., Beijing, China.

Techniques: Immunohistochemistry, Western Blot, Staining, Expressing

Journal: Medical Science Monitor : International Medical Journal of Experimental and Clinical Research

Article Title: Selenium-Rich Diet Induces Myocardial Structural and Functional Abnormalities by Activating Caspase-9 and Caspase-3 in Gpx-1P198L-Overexpression Transgenic Mice

doi: 10.12659/MSM.911120

Figure Lengend Snippet: Expression level of caspase-3 in each group. ( A ) Image results of caspase-9. ( B ) Relative expression level of caspase-3. W1 – wild-type control mice, W2 – wild-type Se-deficient mice, W3 – wild-type Se-supplemented mice, T1 – transgenic control mice, T2 – transgenic Se-deficient mice, T3 – transgenic Se-supplemented mice. * P

Article Snippet: Rabbit anti-caspase-9 and rabbit anti-caspase-3 were diluted in PBST (1: 100 dilution, Wuhan Boster, China).

Techniques: Expressing, Mouse Assay, Transgenic Assay