Journal: Oncology Reports
Article Title: Exosomes derived from retinoblastoma cells enhance tumour deterioration by infiltrating the microenvironment
Figure Lengend Snippet: EXOs derived from WERI-Rb1 cells inhibit innate immunity in vivo . PKH26 EXOs (red) were internalized by cells from spleen tissues (A) 2 or (B) 10 days following EXO injection. Scale bar, 50 or 20 µm. (C) EXOs labelled by PKH26 were engulfed by CD68-positive macrophages in the spleen (green, CD68; red, PKH26; blue, DAPI). Scale bar, 5 µm. (D) Ki-67 (red) and CD45 (green) were labelled by immunofluorescence in spleens from the PBS-injected or EXO-injection groups. Scale bar, 200 or 50 µm. (E) Quantification data showed CD45-positive cells in spleens from the PBS-injected and EXO-injected groups (n=5). (F) Quantification of CD45 and Ki-67 double-positive cells in spleens from the PBS-injected and EXO-injected groups (n=5). (G) Staining of CD49b (red) in spleens from the Sham (normal mice), PBS-injected and EXO-injected groups. Scale bar, 50 µm. (H) Quantification of CD49b-positive cells in spleens from the Sham (n=3), PBS-injected (n=5) and EXO-injected groups (n=5). *P<0.05, **P<0.01 and ***P<0.001. EXO, exosome.
Article Snippet: Samples were incubated with primary antibodies overnight at 4°C, including anti-CD49b for natural killer (NK) cells (1:100; cat. no. 14-5971-85; Invitrogen; Thermo Fisher Scientific, Inc.), anti-CD45 for leukocytes (1:100; cat. no. sc-1178; Santa Cruz Biotechnology, Inc.), anti-CD68 for macrophages (1:100; cat. no. BA3638; Wuhan Boster Biological Technology Co., Ltd.), anti-Ki-67 for proliferative cells (1:500; cat. no. MA5-14520; Thermo Fisher Scientific, Inc.), anti-Vimentin for invasive tumour cells (1:100; cat. no. sc-6260; Santa Cruz Biotechnology, Inc.).
Techniques: Derivative Assay, In Vivo, Injection, Immunofluorescence, Staining