|
Ancell corporation
cd1c Cd1c, supplied by Ancell corporation, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more https://www.bioz.com/result/cd1c/product/Ancell corporation Average 86 stars, based on 1 article reviews Price from $9.99 to $1999.99
cd1c - by Bioz Stars,
2022-08
86/100 stars
|
Buy from Supplier |
|
Thermo Fisher
cd1c Cd1c, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more https://www.bioz.com/result/cd1c/product/Thermo Fisher Average 91 stars, based on 1 article reviews Price from $9.99 to $1999.99
cd1c - by Bioz Stars,
2022-08
91/100 stars
|
Buy from Supplier |
|
Merck & Co
3h2 o 3h2 O, supplied by Merck & Co, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more https://www.bioz.com/result/3h2 o/product/Merck & Co Average 86 stars, based on 1 article reviews Price from $9.99 to $1999.99
3h2 o - by Bioz Stars,
2022-08
86/100 stars
|
Buy from Supplier |
|
Ancell corporation
fitc anti cd1c ![Embryonic DC precursors ingest E. coli , up-regulate costimulatory molecules during culture, and stimulate allogeneic T cells. (A) Phagocytic capacity of leukocytes from embryonic (9–14 wk EGA) and adult skin was assessed by the uptake of <t>FITC-labeled</t> E. coli (10 7 bacteria/2 × 10 6 cells). Flow cytometric analysis revealed exclusive uptake of bacteria in CD45 + HLA-DR + cells (R1). Data are representative of three experiments in embryonic skin and of three experiments in adult skin. (B) Single cells from embryonic skin were cultured for 48 h and expression of CD80, CD86 (donor 1), and CD83 (donor 2) on HLA-DR + cells was analyzed (gated on CD45 + cells). Shown are two representative experiments out of three to five. (C) Allogeneic T cells (5 × 10 4 /well) were cocultured with graded numbers of <t>CD1c</t> + and CD1c − stimulator cells from embryonic skin. T cell proliferation was determined after 5 d by measuring the incorporation of [ 3 H]thymidine. Data are displayed as the mean ± SD of triplicate cultures, and one representative of three independent experiments is shown. (D) Single cell suspensions of embryonic, fetal, and adult skin were cultured for 48 h, and IL-1α and IL-10 levels in supernatants were analyzed by Luminex technology. Bars represent the mean of investigated groups. 9-14 wk EGA: IL-1α and IL-10, n = 12 each. 18–24 wk EGA: IL-1α and IL-10, n = 5 each. Adult: IL-1α and IL-10, n = 7 each.](https://storage.googleapis.com/bioz_article_images/PMC2626673/jem2060169f08.jpg) Fitc Anti Cd1c, supplied by Ancell corporation, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more https://www.bioz.com/result/fitc anti cd1c/product/Ancell corporation Average 86 stars, based on 1 article reviews Price from $9.99 to $1999.99
fitc anti cd1c - by Bioz Stars,
2022-08
86/100 stars
|
Buy from Supplier |
Image Search Results
Journal: The Journal of Experimental Medicine
Article Title: HLA-DR+ leukocytes acquire CD1 antigens in embryonic and fetal human skin and contain functional antigen-presenting cells
doi: 10.1084/jem.20081747
Figure Lengend Snippet: Embryonic DC precursors ingest E. coli , up-regulate costimulatory molecules during culture, and stimulate allogeneic T cells. (A) Phagocytic capacity of leukocytes from embryonic (9–14 wk EGA) and adult skin was assessed by the uptake of FITC-labeled E. coli (10 7 bacteria/2 × 10 6 cells). Flow cytometric analysis revealed exclusive uptake of bacteria in CD45 + HLA-DR + cells (R1). Data are representative of three experiments in embryonic skin and of three experiments in adult skin. (B) Single cells from embryonic skin were cultured for 48 h and expression of CD80, CD86 (donor 1), and CD83 (donor 2) on HLA-DR + cells was analyzed (gated on CD45 + cells). Shown are two representative experiments out of three to five. (C) Allogeneic T cells (5 × 10 4 /well) were cocultured with graded numbers of CD1c + and CD1c − stimulator cells from embryonic skin. T cell proliferation was determined after 5 d by measuring the incorporation of [ 3 H]thymidine. Data are displayed as the mean ± SD of triplicate cultures, and one representative of three independent experiments is shown. (D) Single cell suspensions of embryonic, fetal, and adult skin were cultured for 48 h, and IL-1α and IL-10 levels in supernatants were analyzed by Luminex technology. Bars represent the mean of investigated groups. 9-14 wk EGA: IL-1α and IL-10, n = 12 each. 18–24 wk EGA: IL-1α and IL-10, n = 5 each. Adult: IL-1α and IL-10, n = 7 each.
Article Snippet: Single cell suspensions were stained with the following mAbs: PE anti-Langerin (DCGM4), PE-Cy7 anti-CD45 (J.33; both Beckman Coulter), FITC anti-CD1a (HI-149), FITC anti-CD80 (L307.4), FITC anti-CD83 (HB15e), FITC anti-CD86 (2331), PE anti-CD318 (CUB1), allophycocyanin and PE-anti-HLA-DR (L243; all BD), PE-anti-CD19 (SJ25-C1; Invitrogen), and
Techniques: Labeling, Flow Cytometry, Cell Culture, Expressing, Luminex