Article Title: Inhibition of quantal release from motor nerve by wortmannin
Figure Lengend Snippet: Inhibition of α-latrotoxin-induced mepp burst by wortmannin. (A) The preparation was treated with α-latrotoxin 3 nM for 20 min (top panel), and 40 min later further with wortmannin 100 μM for 220 min (bottom, a different endplate). A part of mepp burst is displayed with a 200 fold expansion of the time scale (middle panel). (B) The preparation was first treated with wortmannin 100 μM for 220 min and then further with α-latrotoxin 10 nM for 40 min (top panel). Bottom panel shows a segment of recording continued from top panel 120 min after washout of α-latrotoxin and wortmannin. Note the significant increase of mepps and partial restoration of mepp burst. Resting membrane potentials were −70∼−82 mV.
Article Snippet: Chemicals μ-Conotoxin GIIIB was purchased from Bachem Feinchemikalien AG (Bubendorf, Switzerland), ω-agatoxin IVA from Peptide Institute Inc. (Osaka, Japan) and α-latrotoxin from Alomone Labs (Jerusalem, Israel).