Journal: ACS Biomaterials Science & Engineering
Article Title: Organotypic 3D Culture in Nanoscaffold Microwells Supports Salivary Gland Stem-Cell-Based Organization
Figure Lengend Snippet: Salivary phenotypic marker and stem cell marker changes at the mRNA level. (A) mRNA levels of salivary acinar cell markers ( AMY1A and AQP5 ), salivary ductal cell markers ( KRT7 , KRT18 , NHE1 , and SLC26C ), intercellular adherence protein ( CDH1 ), and tight junction protein ( TJP ), as well as (B) stem cell-related markers ( POU5F1 , NANOG , LGR5 , THY1 , ITGB1 , HAS , and KRT5 ) were tested by real-time PCR in 2D plastic and 3D spheroid at 3 days after salivary epithelial differentiation. The data from the three independent experiments were analyzed and presented as the mean ± standard errors of the mean ( n = 9). Two-way ANOVA, Bonferroni post hoc test. *, compared with POST in the 2D plastic group; #, compared with POST the floating culture group; $, compared with POST in the Matrigel group. * P
Article Snippet: The blot was incubated overnight at 4 °C in a blocking solution with primary antibodies to the following antigens: α-amylase, AQP5, CK7, CK18, LGR5 and β-actin (Santa Cruz Biotechnology), CD90, KRT5, NANOG, OCT4, and SOX2 (Abcam).
Techniques: Marker, Real-time Polymerase Chain Reaction