LGR5 Antibody Search Results


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  • lgr5  (Abcam)
    95
    Abcam lgr5
    Hepatocyte differentiation reduces the malignant potential of IHCC cells in vitro and in vivo . ( a ) Schedule for hepatocyte differentiation and counting of organoids and spheres. IHCC organoids were cultured in DM or EM for 12 days, then reseeded at 1.0 × 10 3 cells and cultured in EM or Advanced DMEM/F12 (AdDF) with 10% FBS for 10 days. The numbers of organoids and spheres were counted. Scale bars: 500 μl. ( b ) Western blotting of the tumor initiating markers CD44, CD133 and <t>LGR5</t> in IHCC organoids cultured in EM or DM (upper). Immunohistochemical staining of CD44 in IHCC organoids cultured in EM or DM (lower). Scale bars: 50 μm. ( c ) Tumor volumes of xenografted IHCC organoids cultured in EM or DM. We implanted 1 × 10 6 cells of IHCC organoids cultured in EM or DM subcutaneously into the backs of SCID mice. Tumor volumes of xenografted IHCC organoids cultured in EM (n = 8) or DM (n = 8) on SCID mice were measured.
    Lgr5, supplied by Abcam, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Average 95 stars, based on 1 article reviews
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    lgr5 - by Bioz Stars, 2021-05
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    86
    Santa Cruz Biotechnology lgr5
    Salivary phenotypic marker and stem cell marker changes at the mRNA level. (A) mRNA levels of salivary acinar cell markers ( AMY1A and AQP5 ), salivary ductal cell markers ( KRT7 , KRT18 , NHE1 , and SLC26C ), intercellular adherence protein ( CDH1 ), and tight junction protein ( TJP ), as well as (B) stem cell-related markers ( POU5F1 , NANOG , <t>LGR5</t> , THY1 , ITGB1 , HAS , and KRT5 ) were tested by real-time PCR in 2D plastic and 3D spheroid at 3 days after salivary epithelial differentiation. The data from the three independent experiments were analyzed and presented as the mean ± standard errors of the mean ( n = 9). Two-way ANOVA, Bonferroni post hoc test. *, compared with POST in the 2D plastic group; #, compared with POST the floating culture group; $, compared with POST in the Matrigel group. * P
    Lgr5, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/lgr5/product/Santa Cruz Biotechnology
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    lgr5 - by Bioz Stars, 2021-05
    86/100 stars
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    94
    OriGene lgr5 mouse monoclonal antibody
    <t>LGR5</t> regulates proliferation and invasion through Wnt/β-catenin signaling in BGC823 and SGC7901 cells. Cells were transiently transfected with corresponding plasmids. Meanwhile, LGR5 overexpressing cells were treated with Wnt inhibitor C59 and LGR5-knowdown cells were treated with activator Wnt3a. Twenty-four hours after treatment, the cells were examined in the wound healing assay. MTT assays were also started 24 h after treatment. MTT assay ( a , b ) and wound healing assay ( c – f ) were performed as described in Methods. **P
    Lgr5 Mouse Monoclonal Antibody, supplied by OriGene, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/lgr5 mouse monoclonal antibody/product/OriGene
    Average 94 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    lgr5 mouse monoclonal antibody - by Bioz Stars, 2021-05
    94/100 stars
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    N/A
    Rabbit Anti Human LGR5 Polyclonal
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    N/A
    Rabbit polyclonal LGR5 antibody
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    N/A
    Receptor for R spondins that potentiates the canonical Wnt signaling pathway and acts as a stem cell marker of the intestinal epithelium and the hair follicle Upon binding to R
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    N/A
    LGR5 GPR49 is an orphan receptor It may be an important receptor for signals controlling growth and differentiation of specific embryonic tissues
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    Image Search Results


    Hepatocyte differentiation reduces the malignant potential of IHCC cells in vitro and in vivo . ( a ) Schedule for hepatocyte differentiation and counting of organoids and spheres. IHCC organoids were cultured in DM or EM for 12 days, then reseeded at 1.0 × 10 3 cells and cultured in EM or Advanced DMEM/F12 (AdDF) with 10% FBS for 10 days. The numbers of organoids and spheres were counted. Scale bars: 500 μl. ( b ) Western blotting of the tumor initiating markers CD44, CD133 and LGR5 in IHCC organoids cultured in EM or DM (upper). Immunohistochemical staining of CD44 in IHCC organoids cultured in EM or DM (lower). Scale bars: 50 μm. ( c ) Tumor volumes of xenografted IHCC organoids cultured in EM or DM. We implanted 1 × 10 6 cells of IHCC organoids cultured in EM or DM subcutaneously into the backs of SCID mice. Tumor volumes of xenografted IHCC organoids cultured in EM (n = 8) or DM (n = 8) on SCID mice were measured.

    Journal: Scientific Reports

    Article Title: Induction of differentiation of intrahepatic cholangiocarcinoma cells to functional hepatocytes using an organoid culture system

    doi: 10.1038/s41598-018-21121-6

    Figure Lengend Snippet: Hepatocyte differentiation reduces the malignant potential of IHCC cells in vitro and in vivo . ( a ) Schedule for hepatocyte differentiation and counting of organoids and spheres. IHCC organoids were cultured in DM or EM for 12 days, then reseeded at 1.0 × 10 3 cells and cultured in EM or Advanced DMEM/F12 (AdDF) with 10% FBS for 10 days. The numbers of organoids and spheres were counted. Scale bars: 500 μl. ( b ) Western blotting of the tumor initiating markers CD44, CD133 and LGR5 in IHCC organoids cultured in EM or DM (upper). Immunohistochemical staining of CD44 in IHCC organoids cultured in EM or DM (lower). Scale bars: 50 μm. ( c ) Tumor volumes of xenografted IHCC organoids cultured in EM or DM. We implanted 1 × 10 6 cells of IHCC organoids cultured in EM or DM subcutaneously into the backs of SCID mice. Tumor volumes of xenografted IHCC organoids cultured in EM (n = 8) or DM (n = 8) on SCID mice were measured.

    Article Snippet: The membranes were then hybridized with antibodies against CK19 (ab7754, Abcam), CD44 (15675-1-AP, Proteintech), CD133 (W6B3C1, Miltenyi Biotec) and LGR5 (ab75732, Abcam). β-Actin (sc-47778, Santa Cruz Biotechnology) was used as an internal control.

    Techniques: In Vitro, In Vivo, Cell Culture, Western Blot, Immunohistochemistry, Staining, Mouse Assay

    Salivary phenotypic marker and stem cell marker changes at the mRNA level. (A) mRNA levels of salivary acinar cell markers ( AMY1A and AQP5 ), salivary ductal cell markers ( KRT7 , KRT18 , NHE1 , and SLC26C ), intercellular adherence protein ( CDH1 ), and tight junction protein ( TJP ), as well as (B) stem cell-related markers ( POU5F1 , NANOG , LGR5 , THY1 , ITGB1 , HAS , and KRT5 ) were tested by real-time PCR in 2D plastic and 3D spheroid at 3 days after salivary epithelial differentiation. The data from the three independent experiments were analyzed and presented as the mean ± standard errors of the mean ( n = 9). Two-way ANOVA, Bonferroni post hoc test. *, compared with POST in the 2D plastic group; #, compared with POST the floating culture group; $, compared with POST in the Matrigel group. * P

    Journal: ACS Biomaterials Science & Engineering

    Article Title: Organotypic 3D Culture in Nanoscaffold Microwells Supports Salivary Gland Stem-Cell-Based Organization

    doi: 10.1021/acsbiomaterials.8b00894

    Figure Lengend Snippet: Salivary phenotypic marker and stem cell marker changes at the mRNA level. (A) mRNA levels of salivary acinar cell markers ( AMY1A and AQP5 ), salivary ductal cell markers ( KRT7 , KRT18 , NHE1 , and SLC26C ), intercellular adherence protein ( CDH1 ), and tight junction protein ( TJP ), as well as (B) stem cell-related markers ( POU5F1 , NANOG , LGR5 , THY1 , ITGB1 , HAS , and KRT5 ) were tested by real-time PCR in 2D plastic and 3D spheroid at 3 days after salivary epithelial differentiation. The data from the three independent experiments were analyzed and presented as the mean ± standard errors of the mean ( n = 9). Two-way ANOVA, Bonferroni post hoc test. *, compared with POST in the 2D plastic group; #, compared with POST the floating culture group; $, compared with POST in the Matrigel group. * P

    Article Snippet: The blot was incubated overnight at 4 °C in a blocking solution with primary antibodies to the following antigens: α-amylase, AQP5, CK7, CK18, LGR5 and β-actin (Santa Cruz Biotechnology), CD90, KRT5, NANOG, OCT4, and SOX2 (Abcam).

    Techniques: Marker, Real-time Polymerase Chain Reaction

    Salivary phenotypic marker and stem cell marker changes at the protein level after organization induction. The protein translation of the salivary epithelial (α-amylase, AQP5, E-cadherin, ZO-1, CK7, and CK18) or stem cell-related (OCT4, SOX2, NANOG, LGR5, CD90, and KRT5) markers was examined by Western blot, and the expression levels relative to β-actin were calculated. The data from three independent experiments were analyzed and presented as the mean ± standard errors of the mean ( n = 3). One-way ANOVA, Tukey’s post hoc test. *, compared with 2D plastic; #, compared with floating culture; $, compared with Matrigel. *** P

    Journal: ACS Biomaterials Science & Engineering

    Article Title: Organotypic 3D Culture in Nanoscaffold Microwells Supports Salivary Gland Stem-Cell-Based Organization

    doi: 10.1021/acsbiomaterials.8b00894

    Figure Lengend Snippet: Salivary phenotypic marker and stem cell marker changes at the protein level after organization induction. The protein translation of the salivary epithelial (α-amylase, AQP5, E-cadherin, ZO-1, CK7, and CK18) or stem cell-related (OCT4, SOX2, NANOG, LGR5, CD90, and KRT5) markers was examined by Western blot, and the expression levels relative to β-actin were calculated. The data from three independent experiments were analyzed and presented as the mean ± standard errors of the mean ( n = 3). One-way ANOVA, Tukey’s post hoc test. *, compared with 2D plastic; #, compared with floating culture; $, compared with Matrigel. *** P

    Article Snippet: The blot was incubated overnight at 4 °C in a blocking solution with primary antibodies to the following antigens: α-amylase, AQP5, CK7, CK18, LGR5 and β-actin (Santa Cruz Biotechnology), CD90, KRT5, NANOG, OCT4, and SOX2 (Abcam).

    Techniques: Marker, Western Blot, Expressing

    The effect of 3D spheroid culture on gene transcription of SGSCs in floating, Matrigel, and microwell cultures. The mRNA levels of pluripotent stem cell markers ( POU5F1 , SOX2 , and NANOG ), salivary stem/progenitor cell markers ( LGR5 , THY1 , ITGB1 , HAS , and KRT5 ), salivary acinar markers ( AMY1A and AQP5 ), and ductal cell markers ( KRT7 and KRT18 ) were tested by real-time PCR, and the expression levels relative to GAPDH were calculated in triplicate after 1, 3, 5, and 7 days of culture. The data from three independent experiments were analyzed and presented as the mean ± standard errors of the mean ( n = 9). Two-way ANOVA, Bonferroni posthoc test: *compared with 1-day group; # compared with 3-day group; $ compared with 5-day group. * P

    Journal: ACS Biomaterials Science & Engineering

    Article Title: Organotypic 3D Culture in Nanoscaffold Microwells Supports Salivary Gland Stem-Cell-Based Organization

    doi: 10.1021/acsbiomaterials.8b00894

    Figure Lengend Snippet: The effect of 3D spheroid culture on gene transcription of SGSCs in floating, Matrigel, and microwell cultures. The mRNA levels of pluripotent stem cell markers ( POU5F1 , SOX2 , and NANOG ), salivary stem/progenitor cell markers ( LGR5 , THY1 , ITGB1 , HAS , and KRT5 ), salivary acinar markers ( AMY1A and AQP5 ), and ductal cell markers ( KRT7 and KRT18 ) were tested by real-time PCR, and the expression levels relative to GAPDH were calculated in triplicate after 1, 3, 5, and 7 days of culture. The data from three independent experiments were analyzed and presented as the mean ± standard errors of the mean ( n = 9). Two-way ANOVA, Bonferroni posthoc test: *compared with 1-day group; # compared with 3-day group; $ compared with 5-day group. * P

    Article Snippet: The blot was incubated overnight at 4 °C in a blocking solution with primary antibodies to the following antigens: α-amylase, AQP5, CK7, CK18, LGR5 and β-actin (Santa Cruz Biotechnology), CD90, KRT5, NANOG, OCT4, and SOX2 (Abcam).

    Techniques: Real-time Polymerase Chain Reaction, Expressing

    LGR5 regulates proliferation and invasion through Wnt/β-catenin signaling in BGC823 and SGC7901 cells. Cells were transiently transfected with corresponding plasmids. Meanwhile, LGR5 overexpressing cells were treated with Wnt inhibitor C59 and LGR5-knowdown cells were treated with activator Wnt3a. Twenty-four hours after treatment, the cells were examined in the wound healing assay. MTT assays were also started 24 h after treatment. MTT assay ( a , b ) and wound healing assay ( c – f ) were performed as described in Methods. **P

    Journal: Oncogenesis

    Article Title: LGR5 regulates gastric adenocarcinoma cell proliferation and invasion via activating Wnt signaling pathway

    doi: 10.1038/s41389-018-0071-5

    Figure Lengend Snippet: LGR5 regulates proliferation and invasion through Wnt/β-catenin signaling in BGC823 and SGC7901 cells. Cells were transiently transfected with corresponding plasmids. Meanwhile, LGR5 overexpressing cells were treated with Wnt inhibitor C59 and LGR5-knowdown cells were treated with activator Wnt3a. Twenty-four hours after treatment, the cells were examined in the wound healing assay. MTT assays were also started 24 h after treatment. MTT assay ( a , b ) and wound healing assay ( c – f ) were performed as described in Methods. **P

    Article Snippet: The proteins were detected using following antibodies: LGR5 mouse monoclonal antibody (Origene, Rockville, MD, USA), β-actin monoclonal antibody (ZSGB-BIO, Beijing, China).

    Techniques: Transfection, Wound Healing Assay, MTT Assay

    LGR5 regulates expression and location of β-catenin and expression of Wnt/β-catenin target genes in BGC823 and SGC7901 cells. a , b BGC823 and SGC7901 cells were treated with corresponding vectors. Seventy-two hours after transfection, the cells were subjected to immunofluorescent staining for β-catenin (green) and phalloidin staining (red). Nuclei were visualized with DAPI (blue). In control cells (BGC823, BGC823-shRNA-NC and BCG823-NC; SGC7901, SGC7901-shRNA-NC, and SGC7901-NC), β-catenin was with moderate staining distributed in the cytoplasm. When LGR5 was overexpressed, β-catenin staining was increased in cytoplasm and translocated to the nucleus in BCG823 cells. Depleting LGR5 reduced β-catenin level in nucleus and cytoplasm. Scale bar = 10 μm. c , d The relative expression level of β-catenin in nucleus in each group was examined by average fluorescence intensity, and the results are presented graphically as mean ± SD of three independent measurements. **P

    Journal: Oncogenesis

    Article Title: LGR5 regulates gastric adenocarcinoma cell proliferation and invasion via activating Wnt signaling pathway

    doi: 10.1038/s41389-018-0071-5

    Figure Lengend Snippet: LGR5 regulates expression and location of β-catenin and expression of Wnt/β-catenin target genes in BGC823 and SGC7901 cells. a , b BGC823 and SGC7901 cells were treated with corresponding vectors. Seventy-two hours after transfection, the cells were subjected to immunofluorescent staining for β-catenin (green) and phalloidin staining (red). Nuclei were visualized with DAPI (blue). In control cells (BGC823, BGC823-shRNA-NC and BCG823-NC; SGC7901, SGC7901-shRNA-NC, and SGC7901-NC), β-catenin was with moderate staining distributed in the cytoplasm. When LGR5 was overexpressed, β-catenin staining was increased in cytoplasm and translocated to the nucleus in BCG823 cells. Depleting LGR5 reduced β-catenin level in nucleus and cytoplasm. Scale bar = 10 μm. c , d The relative expression level of β-catenin in nucleus in each group was examined by average fluorescence intensity, and the results are presented graphically as mean ± SD of three independent measurements. **P

    Article Snippet: The proteins were detected using following antibodies: LGR5 mouse monoclonal antibody (Origene, Rockville, MD, USA), β-actin monoclonal antibody (ZSGB-BIO, Beijing, China).

    Techniques: Expressing, Transfection, Staining, shRNA, Fluorescence

    LGR5 overexpressing induced morphological changes. Morphology of SGC7901-LGR5 cells was evaluated by phase-contrast microscopy. Overexpression of LGR5 promotes cell morphological changes, acquiring fibroblast-like appearance with spindle shape ( a , b ). Forty-eight hours after transfection, the number of spindle shape cell of SGC7901-LGR5 is significantly more than that of SGC7901-shRNA-LGR5 and control groups. The number of spindle shape and round shape cells in SGC7901-LGR5 is more than that of SGC7901-shRNA-LGR5 and control groups. The SGC7901-LGR5 and SGC7901-shRNA-LGR5 were treated with C59 and Wnt3 to restore the cellular phenotypes. c , d Rhodamine phalloidin staining of actin filament in gastric adenocarcinoma cells SGC7901 and BGC823. Cells were grown to semi-confluency on poly- l -lysine/laminin, coated, fixed, and stained with rhodamine phalloidin as described in Methods. The actin stress fibers (red) were stained throughout the cells. The cell nuclei were counterstained with DAPI (blue). LGR5 overexpression and knockdown cells were also treated with C59 and Wnt3 to restore the cellular phenotypes. Images of close-ups on pseudopods were in the lower panel. Scale bar = 10 μm

    Journal: Oncogenesis

    Article Title: LGR5 regulates gastric adenocarcinoma cell proliferation and invasion via activating Wnt signaling pathway

    doi: 10.1038/s41389-018-0071-5

    Figure Lengend Snippet: LGR5 overexpressing induced morphological changes. Morphology of SGC7901-LGR5 cells was evaluated by phase-contrast microscopy. Overexpression of LGR5 promotes cell morphological changes, acquiring fibroblast-like appearance with spindle shape ( a , b ). Forty-eight hours after transfection, the number of spindle shape cell of SGC7901-LGR5 is significantly more than that of SGC7901-shRNA-LGR5 and control groups. The number of spindle shape and round shape cells in SGC7901-LGR5 is more than that of SGC7901-shRNA-LGR5 and control groups. The SGC7901-LGR5 and SGC7901-shRNA-LGR5 were treated with C59 and Wnt3 to restore the cellular phenotypes. c , d Rhodamine phalloidin staining of actin filament in gastric adenocarcinoma cells SGC7901 and BGC823. Cells were grown to semi-confluency on poly- l -lysine/laminin, coated, fixed, and stained with rhodamine phalloidin as described in Methods. The actin stress fibers (red) were stained throughout the cells. The cell nuclei were counterstained with DAPI (blue). LGR5 overexpression and knockdown cells were also treated with C59 and Wnt3 to restore the cellular phenotypes. Images of close-ups on pseudopods were in the lower panel. Scale bar = 10 μm

    Article Snippet: The proteins were detected using following antibodies: LGR5 mouse monoclonal antibody (Origene, Rockville, MD, USA), β-actin monoclonal antibody (ZSGB-BIO, Beijing, China).

    Techniques: Microscopy, Over Expression, Transfection, shRNA, Staining

    Overexpression and knockdown efficiency of LGR5 were analyzed by western blot. SGC7901 ( a ) or BGC823 ( b ) cells were treated with pGPU6/GFP/Neo containing shRNA to NC sequences, to LGR5 targeting sequence or with pReceiver-M45-LGR5 or pReceiver-M45 as a control. Expression of LGR5 was assessed by western blot (right panels) 72 h after transfection. The band densities were measured by NIH Image J (left panels). The expression levels of LGR5 in parental SGC7901 and BGC823 were considered as “1”

    Journal: Oncogenesis

    Article Title: LGR5 regulates gastric adenocarcinoma cell proliferation and invasion via activating Wnt signaling pathway

    doi: 10.1038/s41389-018-0071-5

    Figure Lengend Snippet: Overexpression and knockdown efficiency of LGR5 were analyzed by western blot. SGC7901 ( a ) or BGC823 ( b ) cells were treated with pGPU6/GFP/Neo containing shRNA to NC sequences, to LGR5 targeting sequence or with pReceiver-M45-LGR5 or pReceiver-M45 as a control. Expression of LGR5 was assessed by western blot (right panels) 72 h after transfection. The band densities were measured by NIH Image J (left panels). The expression levels of LGR5 in parental SGC7901 and BGC823 were considered as “1”

    Article Snippet: The proteins were detected using following antibodies: LGR5 mouse monoclonal antibody (Origene, Rockville, MD, USA), β-actin monoclonal antibody (ZSGB-BIO, Beijing, China).

    Techniques: Over Expression, Western Blot, shRNA, Sequencing, Expressing, Transfection

    Overexpression and knockdown of LGR5 have opposite effects on cell proliferation. a , c Gastric adenocarcinoma cells were plated 24 h after transfection with 1.5 × 10 3 cells/well in six-well plates for 14 days then tested for their ability of clonogenicity as described in Methods. The colonies (≥50 cells) were numbered. Representative images of colonies in plates stained with Giemsa. Images were taken using a Nikon 90i with a DXM 1200C camera. b , d Data are presented as values of mean ± SD from three independent measurements and the asterisk indicates statistical significance compared with the control (untransfected) parental cells. P -values were calculated with Student’s t test. ** P

    Journal: Oncogenesis

    Article Title: LGR5 regulates gastric adenocarcinoma cell proliferation and invasion via activating Wnt signaling pathway

    doi: 10.1038/s41389-018-0071-5

    Figure Lengend Snippet: Overexpression and knockdown of LGR5 have opposite effects on cell proliferation. a , c Gastric adenocarcinoma cells were plated 24 h after transfection with 1.5 × 10 3 cells/well in six-well plates for 14 days then tested for their ability of clonogenicity as described in Methods. The colonies (≥50 cells) were numbered. Representative images of colonies in plates stained with Giemsa. Images were taken using a Nikon 90i with a DXM 1200C camera. b , d Data are presented as values of mean ± SD from three independent measurements and the asterisk indicates statistical significance compared with the control (untransfected) parental cells. P -values were calculated with Student’s t test. ** P

    Article Snippet: The proteins were detected using following antibodies: LGR5 mouse monoclonal antibody (Origene, Rockville, MD, USA), β-actin monoclonal antibody (ZSGB-BIO, Beijing, China).

    Techniques: Over Expression, Transfection, Staining

    Effect of LGR5 on cell migration in gastric adenocarcinoma cell lines (SGC7901 and BGC823). a , c SGC7901 and BGC823 cells were transiently transfected with pGPU6/GFP/Neo-shRNA-LGR5, pGPU6/GFP/Neo-shRNA-NC, pReceiver-M45-LGR5, and pReceiver-M45-NC respectively. After 24 h transfection, the cells were grown to 80 % confluence at which time cells were removed with a 10 μl tip in the adherent monolayers. The cells were recovered for 48 h. Images were acquired at 0, 24, and 48 h following the recovery with Olympus digital camera, and analyzed by Image J to measure ability of the cells to repair the wound. b , d Cellular migration was quantified and represented graphically with results expressed as mean ± SD of three independent measurements. **P

    Journal: Oncogenesis

    Article Title: LGR5 regulates gastric adenocarcinoma cell proliferation and invasion via activating Wnt signaling pathway

    doi: 10.1038/s41389-018-0071-5

    Figure Lengend Snippet: Effect of LGR5 on cell migration in gastric adenocarcinoma cell lines (SGC7901 and BGC823). a , c SGC7901 and BGC823 cells were transiently transfected with pGPU6/GFP/Neo-shRNA-LGR5, pGPU6/GFP/Neo-shRNA-NC, pReceiver-M45-LGR5, and pReceiver-M45-NC respectively. After 24 h transfection, the cells were grown to 80 % confluence at which time cells were removed with a 10 μl tip in the adherent monolayers. The cells were recovered for 48 h. Images were acquired at 0, 24, and 48 h following the recovery with Olympus digital camera, and analyzed by Image J to measure ability of the cells to repair the wound. b , d Cellular migration was quantified and represented graphically with results expressed as mean ± SD of three independent measurements. **P

    Article Snippet: The proteins were detected using following antibodies: LGR5 mouse monoclonal antibody (Origene, Rockville, MD, USA), β-actin monoclonal antibody (ZSGB-BIO, Beijing, China).

    Techniques: Migration, Transfection, shRNA

    Effects of LGR5 modulation on invasion by transwell assay. a , c Cells were seeded in Transwell chambers (8 μm pore size) 24 h after transfection, samples were harvested after additional 24 h. Migrated cells were fixed and stained with Crystal Violet. Cells presented on the underside of the filters were counted by a light microscope (Olympus) at ×200 magnification. b , d Transmigration cells were counted for each of the indicated cells. The graph presented mean ± SD of three separate samples for each cell type; the parental cells were used as the control. Significance levels were determined by the Student’s t -test. **P

    Journal: Oncogenesis

    Article Title: LGR5 regulates gastric adenocarcinoma cell proliferation and invasion via activating Wnt signaling pathway

    doi: 10.1038/s41389-018-0071-5

    Figure Lengend Snippet: Effects of LGR5 modulation on invasion by transwell assay. a , c Cells were seeded in Transwell chambers (8 μm pore size) 24 h after transfection, samples were harvested after additional 24 h. Migrated cells were fixed and stained with Crystal Violet. Cells presented on the underside of the filters were counted by a light microscope (Olympus) at ×200 magnification. b , d Transmigration cells were counted for each of the indicated cells. The graph presented mean ± SD of three separate samples for each cell type; the parental cells were used as the control. Significance levels were determined by the Student’s t -test. **P

    Article Snippet: The proteins were detected using following antibodies: LGR5 mouse monoclonal antibody (Origene, Rockville, MD, USA), β-actin monoclonal antibody (ZSGB-BIO, Beijing, China).

    Techniques: Transwell Assay, Transfection, Staining, Light Microscopy, Transmigration Assay