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  • 86
    Qiagen il4i1
    Main Protein Clusters Resulting from String Network Analysis. STRING network analysis was performed for significantly differentially abundant proteins ( n = 154) considering only highest confidence interactions (interaction score 0.9, text mining as a source of interaction was disabled). The analysis resulted in two main protein clusters, one of them representing proteins related to oxidative phosphorylation (blue circles). The second cluster (green circles) contained proteins related to oxidation-reduction processes, one of them the most strikingly upregulated protein <t>IL4I1</t> (highlighted in red). Gene names are given next to the protein symbols, ratios of means are indicated next to the gene names, green arrows indicate higher protein abundance in the A. fumigatus infected group. Edges represent protein-protein interactions with the color code given next to the network.
    Il4i1, supplied by Qiagen, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/il4i1/product/Qiagen
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    il4i1 - by Bioz Stars, 2021-05
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    86
    Boston Scientific watchman laao
    Main Protein Clusters Resulting from String Network Analysis. STRING network analysis was performed for significantly differentially abundant proteins ( n = 154) considering only highest confidence interactions (interaction score 0.9, text mining as a source of interaction was disabled). The analysis resulted in two main protein clusters, one of them representing proteins related to oxidative phosphorylation (blue circles). The second cluster (green circles) contained proteins related to oxidation-reduction processes, one of them the most strikingly upregulated protein <t>IL4I1</t> (highlighted in red). Gene names are given next to the protein symbols, ratios of means are indicated next to the gene names, green arrows indicate higher protein abundance in the A. fumigatus infected group. Edges represent protein-protein interactions with the color code given next to the network.
    Watchman Laao, supplied by Boston Scientific, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/watchman laao/product/Boston Scientific
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    watchman laao - by Bioz Stars, 2021-05
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    96
    R&D Systems recombinant mouse il4i1
    <t>IL4I1</t> injection rescues remyelination impairment in Il4ra − / − mice. Quantification of ( A ) CC1 + Olig2 + cells, ( B ) SMI-32 signal intensity normalized to NF200, and ( C ) CD11b + iNOS + macrophages in Il4ra −/− and Il4ra −/− treated mice at 10 dpl. * P
    Recombinant Mouse Il4i1, supplied by R&D Systems, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/recombinant mouse il4i1/product/R&D Systems
    Average 96 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    recombinant mouse il4i1 - by Bioz Stars, 2021-05
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    86
    Abbott Laboratories amplatzer amulet laao device
    <t>IL4I1</t> injection rescues remyelination impairment in Il4ra − / − mice. Quantification of ( A ) CC1 + Olig2 + cells, ( B ) SMI-32 signal intensity normalized to NF200, and ( C ) CD11b + iNOS + macrophages in Il4ra −/− and Il4ra −/− treated mice at 10 dpl. * P
    Amplatzer Amulet Laao Device, supplied by Abbott Laboratories, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/amplatzer amulet laao device/product/Abbott Laboratories
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    amplatzer amulet laao device - by Bioz Stars, 2021-05
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    N/A
    Target species mouse Gene Silencers generally consist of pools of three to five target specific 19 25 nucleotide sequences in length For independent verification of LAAO gene silencing results individual
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    N/A
    Target species mouse Gene Silencers generally consist of pools of three to five target specific 19 25 nucleotide sequences in length For independent verification of LAAO gene silencing results individual
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    N/A
    Target species mouse CRISPR Cas9 KO Plasmids consists of LAAO specific 20 nt guide RNA sequences derived from the GeCKO v2 library For CRISPR gene knockout gRNA sequences direct the
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    Target species mouse CRISPR Cas9 KO Plasmids consists of LAAO specific 20 nt guide RNA sequences derived from the GeCKO v2 library For CRISPR gene knockout gRNA sequences direct the
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    Image Search Results


    Main Protein Clusters Resulting from String Network Analysis. STRING network analysis was performed for significantly differentially abundant proteins ( n = 154) considering only highest confidence interactions (interaction score 0.9, text mining as a source of interaction was disabled). The analysis resulted in two main protein clusters, one of them representing proteins related to oxidative phosphorylation (blue circles). The second cluster (green circles) contained proteins related to oxidation-reduction processes, one of them the most strikingly upregulated protein IL4I1 (highlighted in red). Gene names are given next to the protein symbols, ratios of means are indicated next to the gene names, green arrows indicate higher protein abundance in the A. fumigatus infected group. Edges represent protein-protein interactions with the color code given next to the network.

    Journal: Molecular & Cellular Proteomics : MCP

    Article Title: Quantitative Analysis of Proteome Modulations in Alveolar Epithelial Type II Cells in Response to Pulmonary Aspergillus fumigatus Infection *

    doi: 10.1074/mcp.RA117.000072

    Figure Lengend Snippet: Main Protein Clusters Resulting from String Network Analysis. STRING network analysis was performed for significantly differentially abundant proteins ( n = 154) considering only highest confidence interactions (interaction score 0.9, text mining as a source of interaction was disabled). The analysis resulted in two main protein clusters, one of them representing proteins related to oxidative phosphorylation (blue circles). The second cluster (green circles) contained proteins related to oxidation-reduction processes, one of them the most strikingly upregulated protein IL4I1 (highlighted in red). Gene names are given next to the protein symbols, ratios of means are indicated next to the gene names, green arrows indicate higher protein abundance in the A. fumigatus infected group. Edges represent protein-protein interactions with the color code given next to the network.

    Article Snippet: Here, we show for the first time that IL4I1 is present in primary murine AEC II during A. fumigatus infection.

    Techniques: Infection

    IL4I1 injection rescues remyelination impairment in Il4ra − / − mice. Quantification of ( A ) CC1 + Olig2 + cells, ( B ) SMI-32 signal intensity normalized to NF200, and ( C ) CD11b + iNOS + macrophages in Il4ra −/− and Il4ra −/− treated mice at 10 dpl. * P

    Journal: Brain

    Article Title: IL4I1 augments CNS remyelination and axonal protection by modulating T cell driven inflammation

    doi: 10.1093/brain/aww254

    Figure Lengend Snippet: IL4I1 injection rescues remyelination impairment in Il4ra − / − mice. Quantification of ( A ) CC1 + Olig2 + cells, ( B ) SMI-32 signal intensity normalized to NF200, and ( C ) CD11b + iNOS + macrophages in Il4ra −/− and Il4ra −/− treated mice at 10 dpl. * P

    Article Snippet: For treated animals, 200 ng/ml of recombinant mouse IL4I1 (R & D Systems) was co-injected along with 1.0% lysolecithin into the ventral spinal cord.

    Techniques: Injection, Mouse Assay

    IL4I1 regulates remyelination and preserves axonal integrity. Quantification of ( A ) Nkx2.2 + Olig2 + and ( B ) CC1 + Olig2 + cells per mm 2 in lesions at 5, 10 and 20 dpl in wild-type (WT), Il4i1 − / − and IL4I1-treated mice. ( C ) Immunostaining of Olig2 (green), CC1 (red) and DAPI (blue) in lesions at 10 dpl. Lesions are characterized as the cluster of DAPI + nuclei in the spinal cord ventral funiculous. ( D ) Electron micrographs of lesion at 10 dpl show reduced remyelinated axons in Il4i1 − / − lesions, and increased remyelinated axons in IL4I1 treated lesions compared to wild-type. ( E ) G-ratio analysis of remyelinated axons and corresponding axonal diameter. ( F ) Scatter plot showing the overall g-ratio in the mouse groups. ( G ) Electron micrographs of lesions in Il4i1 − / − mice showing a dystrophic axon and an axon that has undergone Wallerian degeneration. ( H ) Immunostaining of SMI-32 (green), NF200 (red) and DAPI (blue) in spinal cord lesions at 10 dpl. Axonal dystrophy is detected by SMI-32 + NF200 + co-labelling. ( I ) Graph of relative SMI-32 signal intensity normalized to NF200. For cell counts and fluorescence intensity, n = 3–5 mice per group were used and n = 3 ×10 magnification images per mouse were analysed. Scale bar = 100 μm for immunofluorescence images. * P

    Journal: Brain

    Article Title: IL4I1 augments CNS remyelination and axonal protection by modulating T cell driven inflammation

    doi: 10.1093/brain/aww254

    Figure Lengend Snippet: IL4I1 regulates remyelination and preserves axonal integrity. Quantification of ( A ) Nkx2.2 + Olig2 + and ( B ) CC1 + Olig2 + cells per mm 2 in lesions at 5, 10 and 20 dpl in wild-type (WT), Il4i1 − / − and IL4I1-treated mice. ( C ) Immunostaining of Olig2 (green), CC1 (red) and DAPI (blue) in lesions at 10 dpl. Lesions are characterized as the cluster of DAPI + nuclei in the spinal cord ventral funiculous. ( D ) Electron micrographs of lesion at 10 dpl show reduced remyelinated axons in Il4i1 − / − lesions, and increased remyelinated axons in IL4I1 treated lesions compared to wild-type. ( E ) G-ratio analysis of remyelinated axons and corresponding axonal diameter. ( F ) Scatter plot showing the overall g-ratio in the mouse groups. ( G ) Electron micrographs of lesions in Il4i1 − / − mice showing a dystrophic axon and an axon that has undergone Wallerian degeneration. ( H ) Immunostaining of SMI-32 (green), NF200 (red) and DAPI (blue) in spinal cord lesions at 10 dpl. Axonal dystrophy is detected by SMI-32 + NF200 + co-labelling. ( I ) Graph of relative SMI-32 signal intensity normalized to NF200. For cell counts and fluorescence intensity, n = 3–5 mice per group were used and n = 3 ×10 magnification images per mouse were analysed. Scale bar = 100 μm for immunofluorescence images. * P

    Article Snippet: For treated animals, 200 ng/ml of recombinant mouse IL4I1 (R & D Systems) was co-injected along with 1.0% lysolecithin into the ventral spinal cord.

    Techniques: Mouse Assay, Immunostaining, Fluorescence, Immunofluorescence

    Il4i1 is upregulated in alternatively activated microglia and macrophages through IL-4 receptor signalling. qRT-PCR detection of Il4i1 in untreated, lipopolysaccharide (LPS)- and IL4-treated ( A ) microglia, ( B ) BV2 cells, and ( C ) RAW264.7 cell at 24 h after treatment ( n = 3 per group). ( D ) qRT-PCR for Il4i1 expression in unlesioned wild-type (WT), lesioned wild-type and lesioned II4ra −/− spinal cord at 10 dpl ( n = 3 per group). ( E ) In situ hybridization of Il4i1 in wild-type and II4ra −/− mice at 10 dpl. Spinal cord lesion is encircled. GM = grey matter, WM = white matter. Scale bar = 100 μm. Density of ( F ) oligodendrocyte precursor cells (PDGFRα + Olig2 + ) and ( G ) oligodendrocytes (CC1 + Olig2 + ) from MACS purified primary oligodendrocyte precursor cell cultures at 3 days in vitro after treatment with vehicle (1XPBS) or recombinant IL4I1 for 24 h ( n = 5 images per condition). All experimental results were replicated at least twice. ** P

    Journal: Brain

    Article Title: IL4I1 augments CNS remyelination and axonal protection by modulating T cell driven inflammation

    doi: 10.1093/brain/aww254

    Figure Lengend Snippet: Il4i1 is upregulated in alternatively activated microglia and macrophages through IL-4 receptor signalling. qRT-PCR detection of Il4i1 in untreated, lipopolysaccharide (LPS)- and IL4-treated ( A ) microglia, ( B ) BV2 cells, and ( C ) RAW264.7 cell at 24 h after treatment ( n = 3 per group). ( D ) qRT-PCR for Il4i1 expression in unlesioned wild-type (WT), lesioned wild-type and lesioned II4ra −/− spinal cord at 10 dpl ( n = 3 per group). ( E ) In situ hybridization of Il4i1 in wild-type and II4ra −/− mice at 10 dpl. Spinal cord lesion is encircled. GM = grey matter, WM = white matter. Scale bar = 100 μm. Density of ( F ) oligodendrocyte precursor cells (PDGFRα + Olig2 + ) and ( G ) oligodendrocytes (CC1 + Olig2 + ) from MACS purified primary oligodendrocyte precursor cell cultures at 3 days in vitro after treatment with vehicle (1XPBS) or recombinant IL4I1 for 24 h ( n = 5 images per condition). All experimental results were replicated at least twice. ** P

    Article Snippet: For treated animals, 200 ng/ml of recombinant mouse IL4I1 (R & D Systems) was co-injected along with 1.0% lysolecithin into the ventral spinal cord.

    Techniques: Quantitative RT-PCR, Expressing, In Situ Hybridization, Mouse Assay, Magnetic Cell Separation, Purification, In Vitro, Recombinant

    Il4i1 is expressed during remyelination in CNS lesions. ( A ) Graph showing the differential expression pattern of Il4i1 against Mmp12 , and Mbp at 5, 14 and 28 dpl. The values were obtained from a previously published rat remyelination transcriptome ( Huang et al. , 2011 ). ( B ) qRT-PCR detection of Il4i1 in lesioned mouse spinal cord at 3, 10 and 20 dpl compared to control non-lesioned tissue ( n = 3 per group). ( C ) In situ hybridization of Il4i1 in a non-lesioned and focally demyelinated mouse spinal cord sections at 10 dpl. Sense control staining shows faint labelling. GM = grey matter; WM = white matter. Scale bar = 100 μm. * P

    Journal: Brain

    Article Title: IL4I1 augments CNS remyelination and axonal protection by modulating T cell driven inflammation

    doi: 10.1093/brain/aww254

    Figure Lengend Snippet: Il4i1 is expressed during remyelination in CNS lesions. ( A ) Graph showing the differential expression pattern of Il4i1 against Mmp12 , and Mbp at 5, 14 and 28 dpl. The values were obtained from a previously published rat remyelination transcriptome ( Huang et al. , 2011 ). ( B ) qRT-PCR detection of Il4i1 in lesioned mouse spinal cord at 3, 10 and 20 dpl compared to control non-lesioned tissue ( n = 3 per group). ( C ) In situ hybridization of Il4i1 in a non-lesioned and focally demyelinated mouse spinal cord sections at 10 dpl. Sense control staining shows faint labelling. GM = grey matter; WM = white matter. Scale bar = 100 μm. * P

    Article Snippet: For treated animals, 200 ng/ml of recombinant mouse IL4I1 (R & D Systems) was co-injected along with 1.0% lysolecithin into the ventral spinal cord.

    Techniques: Expressing, Quantitative RT-PCR, In Situ Hybridization, Staining

    IL4I1 modulates IFN-γ and IL-17 expression in CNS lesions and splenocytes. ( A ) Normalized expressions of Ifng , Il17 and Il4 in wild-type (WT, n = 4) and IL4I1-treated ( n = 6) spinal cord lesions at 10 dpl by qRT-PCR. ( B ) Normalized expression of Ifng expression in splenocyte cultures treated with PMA+Ionomycin in the presence or absence of recombinant IL4I1 for 24 h ( n = 3 per group) followed by qRT-PCR. ( C ) Cell death assay in splenocyte cultures treated with PMA+Ionomycin with or without recombinant IL4I1 for 24 h, and assessed by LIVE/DEAD ® cell stain kit ( n = 8 per group).

    Journal: Brain

    Article Title: IL4I1 augments CNS remyelination and axonal protection by modulating T cell driven inflammation

    doi: 10.1093/brain/aww254

    Figure Lengend Snippet: IL4I1 modulates IFN-γ and IL-17 expression in CNS lesions and splenocytes. ( A ) Normalized expressions of Ifng , Il17 and Il4 in wild-type (WT, n = 4) and IL4I1-treated ( n = 6) spinal cord lesions at 10 dpl by qRT-PCR. ( B ) Normalized expression of Ifng expression in splenocyte cultures treated with PMA+Ionomycin in the presence or absence of recombinant IL4I1 for 24 h ( n = 3 per group) followed by qRT-PCR. ( C ) Cell death assay in splenocyte cultures treated with PMA+Ionomycin with or without recombinant IL4I1 for 24 h, and assessed by LIVE/DEAD ® cell stain kit ( n = 8 per group).

    Article Snippet: For treated animals, 200 ng/ml of recombinant mouse IL4I1 (R & D Systems) was co-injected along with 1.0% lysolecithin into the ventral spinal cord.

    Techniques: Expressing, Quantitative RT-PCR, Recombinant, Staining

    IL4I1 reverses clinical severity and preserves axons in mice with EAE. ( A ) Clinical scores of PBS-treated ( n = 8) and IL4I1-treated ( n = 10) mice for 28 days after EAE induction. Flow cytometry analysis of ( B ) spinal cord and ( C ) spleen from untreated and IL4I1 treated mice at 35 days after EAE induction showing the percentage and total number of gated T-bet + CD4 + , Rorγt + CD4 + and Gata3 + CD4 + cells (samples from n = 2 mice were combined for each group and analysed). ( D ) Immunostaining of SMI-32 (green), NF200 (red) and DAPI (blue) in spinal cord of untreated and IL4I1 treated mice with EAE at 35 days after EAE induction. Axonal dystrophy is detected by SMI-32 + NF200 + co-labelling. ( E ) SMI-32 signal intensity normalized to NF200 in untreated, and IL4I1-treated mice showing reduced axonal dystrophy in IL4I1 treated mice ( n = 3 per group). Scale bar = 100 μm. * P

    Journal: Brain

    Article Title: IL4I1 augments CNS remyelination and axonal protection by modulating T cell driven inflammation

    doi: 10.1093/brain/aww254

    Figure Lengend Snippet: IL4I1 reverses clinical severity and preserves axons in mice with EAE. ( A ) Clinical scores of PBS-treated ( n = 8) and IL4I1-treated ( n = 10) mice for 28 days after EAE induction. Flow cytometry analysis of ( B ) spinal cord and ( C ) spleen from untreated and IL4I1 treated mice at 35 days after EAE induction showing the percentage and total number of gated T-bet + CD4 + , Rorγt + CD4 + and Gata3 + CD4 + cells (samples from n = 2 mice were combined for each group and analysed). ( D ) Immunostaining of SMI-32 (green), NF200 (red) and DAPI (blue) in spinal cord of untreated and IL4I1 treated mice with EAE at 35 days after EAE induction. Axonal dystrophy is detected by SMI-32 + NF200 + co-labelling. ( E ) SMI-32 signal intensity normalized to NF200 in untreated, and IL4I1-treated mice showing reduced axonal dystrophy in IL4I1 treated mice ( n = 3 per group). Scale bar = 100 μm. * P

    Article Snippet: For treated animals, 200 ng/ml of recombinant mouse IL4I1 (R & D Systems) was co-injected along with 1.0% lysolecithin into the ventral spinal cord.

    Techniques: Mouse Assay, Flow Cytometry, Cytometry, Immunostaining

    IL4I1 modulates inflammation in CNS lesions. Quantification of macrophage subpopulations in lesions of wild-type, Il4i1 − / − and IL4I1 treated mice at 5, 10 and 20 dpl. ( A ) CD11b + iNOS + cell quantification. ( B ) Immunostaining of iNOS (green), CD11b (red) and DAPI (blue) at 10 dpl. ( C ) CD11b + Ym1 + cell quantification. ( D ) Immunostaining of Ym1 (green), CD11b (red) and DAPI (blue) at 10 dpl. ( E ) Quantification of the ratio of iNOS + /Ym1 + cells in lesions. ( F ) Flow cytometry analysis of iNOS + CD11b + cells in lesioned spinal cord of wild-type (WT) and IL4I1-treated mice at 10 dpl. For cell counts, n = 3–5 mice per group were used and n = 3 × 10 magnification images per mouse were analysed. Scale bar = 100 μm.

    Journal: Brain

    Article Title: IL4I1 augments CNS remyelination and axonal protection by modulating T cell driven inflammation

    doi: 10.1093/brain/aww254

    Figure Lengend Snippet: IL4I1 modulates inflammation in CNS lesions. Quantification of macrophage subpopulations in lesions of wild-type, Il4i1 − / − and IL4I1 treated mice at 5, 10 and 20 dpl. ( A ) CD11b + iNOS + cell quantification. ( B ) Immunostaining of iNOS (green), CD11b (red) and DAPI (blue) at 10 dpl. ( C ) CD11b + Ym1 + cell quantification. ( D ) Immunostaining of Ym1 (green), CD11b (red) and DAPI (blue) at 10 dpl. ( E ) Quantification of the ratio of iNOS + /Ym1 + cells in lesions. ( F ) Flow cytometry analysis of iNOS + CD11b + cells in lesioned spinal cord of wild-type (WT) and IL4I1-treated mice at 10 dpl. For cell counts, n = 3–5 mice per group were used and n = 3 × 10 magnification images per mouse were analysed. Scale bar = 100 μm.

    Article Snippet: For treated animals, 200 ng/ml of recombinant mouse IL4I1 (R & D Systems) was co-injected along with 1.0% lysolecithin into the ventral spinal cord.

    Techniques: Mouse Assay, Immunostaining, Flow Cytometry, Cytometry