Alomone Labs
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Image Search Results

Journal:
Article Title: Brain-derived neurotrophic factor attenuates mouse cerebellar granule cell GABA A receptor-mediated responses via postsynaptic mechanisms
doi: 10.1113/jphysiol.2002.037846
Figure Lengend Snippet: A, peak amplitude of responses to GABA (20 μm) plotted as a function of time before, during and after exposure to 100 ng ml−1 BDNF. The continuous line denotes time and duration of BDNF application. GABA responses were monitored in normal bath solution without exposure to BDNF during recording (□), in normal bath solution with a 1 min exposure to BDNF (▪), and with added K252a (200 nm; ○), K252b (200 nm; •) and TrkB-IgG (5 μg ml−1; ▵). Each point represents data averaged from 8–10 cells. B, bar graph summarizing mean peak amplitude of GABA responses as a function of the conditions in A.
Article Snippet: Recombinant human BDNF was purchased from Peprotech Inc. (Rocky Hill, NJ, USA); TrkB-Fc protein was a gift from Regeneron (Tarrytown, NY, USA); K252a and
Techniques:

Journal:
Article Title: Brain-derived neurotrophic factor attenuates mouse cerebellar granule cell GABA A receptor-mediated responses via postsynaptic mechanisms
doi: 10.1113/jphysiol.2002.037846
Figure Lengend Snippet: Confocal images of cultured cerebellar granule cells before (A) and following a 60 min exposure to BDNF (100 ng ml−1) (B, C and D). The cultures were fixed and co-stained with antibodies against GABAA receptor β2/3 subunits (green fluorescence) and TrkB receptors (red fluorescence). GABAA receptor β2/3 subunit immunoreactivity, illustrated in A, is markedly reduced following exposure to BDNF (B). C and D, addition of K252a (200 nm) prevented the BDNF-induced reduction of GABAA receptor β2/3 subunit immunoreactivity (C), which persisted in the presence of K252b (200 nm; D). Scale bar (applies to all panels), 50 μm.
Article Snippet: Recombinant human BDNF was purchased from Peprotech Inc. (Rocky Hill, NJ, USA); TrkB-Fc protein was a gift from Regeneron (Tarrytown, NY, USA); K252a and
Techniques: Cell Culture, Staining, Fluorescence